Physiological and behavioural parameters of broiler chicks grown under different heating systems

Received: December 1st, 2022 ; Accepted: March 29th, 2023 ; Published: April 24th , 2023 ; Correspondence: [email protected] study aimed to compare the internal environment, physiological variables, and behavioural responses of chicks under two different heating systems. The experiment was performed in two commercial broiler houses located in Brazil, where 28,000 male Cobb chicks were housed during the first three weeks of life. One of the broiler houses was heated by an industrial indirect-fired biomass furnace (S1). In addition, another heating system consisting of two furnaces for indirect heating of the air using biomass (wood) as fuel, built by hand with bricks, mud, and dung on an iron structure (S2), was tested. Measurements of the dry bulb temperature (tdb), dew point temperature (tdp), and relative air humidity (RH) were performed. Subsequently, the temperature-humidity index (THI) was calculated. In addition, the physiological variables of the respiratory rate (RR) and cloacal temperature (tcloacal) were measured three times a day (8:00 am, 2:00 pm, and 6:00 pm) in four chicks. The behaviours were grouped by dendrograms, in which the similarity of these data was qualified. During the second and third weeks of life, the THI values were below the recommended range. The RR and tcloacal data were below the recommended comfort values, which may be an indicator that the chicks were subjected to cold conditions. Regarding their behaviour, the chicks exhibited calm, feeding, and sleepy behaviours most of the time. Problems in the heating system inside the broiler house could be observed, possibly affecting the chicks’ thermal comfort and welfare, which consequently can result in productive and economic losses

Differential pain-related behaviors and bone disease in immunocompetent mouse models of myeloma

Bone pain is a serious and debilitating symptom of multiple myeloma (MM) that impairs the quality of life of patients. The underlying mechanisms of the pain are unknown and understudied, and there is a need for immunocompetent preclinical models of myeloma‐induced bone pain. The aim of this study was to provide the first in‐depth behavioral characterization of an immunocompetent mouse model of MM presenting the clinical disease features: osteolytic bone disease and bone pain. We hypothesized that a widely used syngeneic model of MM, established by systemic inoculation of green fluorescent protein‐tagged myeloma cells (5TGM1‐GFP) in immunocompetent C57Bl/KaLwRijHsd (BKAL) mice, would present pain‐related behaviors. Disease phenotype was confirmed by splenomegaly, high serum paraprotein, and tumor infiltration in the bone marrow of the hind limbs; however, myeloma‐bearing mice did not present pain‐related behaviors or substantial bone disease. Thus, we investigated an alternative model in which 5TGM1‐GFP cells were directly inoculated into the intrafemoral medullary cavity. This localized myeloma model presented the hallmarks of the disease, including high serum paraprotein, tumor growth, and osteolytic bone lesions. Compared with control mice, myeloma‐bearing mice presented myeloma‐induced pain‐related behaviors, a phenotype that was reversed by systemic morphine treatment. Micro‐computed tomography analyses of the myeloma‐inoculated femurs showed bone disease in cortical and trabecular bone. Repeated systemic bisphosphonate treatment induced an amelioration of the nociceptive phenotype, but did not completely reverse it. Furthermore, intrafemorally injected mice presented a profound denervation of the myeloma‐bearing bones, a previously unknown feature of the disease. This study reports the intrafemoral inoculation of 5TGM1‐GFP cells as a robust immunocompetent model of myeloma‐induced bone pain, with consistent bone loss. Moreover, the data suggest that myeloma‐induced bone pain is caused by a combinatorial mechanism including osteolysis and bone marrow denervation. © 2019 The Authors. JBMR Plus published by Wiley Periodicals, Inc. on behalf of American Society for Bone and Mineral Research

Anatomical Features Of The Urethra And Urinary Bladder Catheterization In Female Mice And Rats. An Essential Translational Tool [características Anatômicas Da Cateterização Da Uretra E Bexiga De Camundongos E Ratos Fêmeas. Instrumento Essencial Na Pesquisa Pré Clínica]

PURPOSE: To present fundamental anatomical aspects and technical skills necessary to urethra and urinary bladder catheterization in female mice and rats. METHODS: Urethral and bladder catheterization has been widely utilized for carcinogenesis and cancer research and still remains very useful in several applications: from toxicological purposes as well as inflammatory and infectious conditions to functional aspects as bladder dynamics and vesicoureteral reflux, among many others. RESULTS: Animal models are in the center of translational research and those involving rodents are the most important nowadays due to several advantages including human reproducibility, easy handling and low cost. CONCLUSIONS: Although technical and anatomical pearls for rodent urethral and bladder access are presented as tackles to the advancement of lower urinary tract preclinical investigation in a broaden sight, restriction to female animals hampers the male microenvironment, demanding future advances.26SUPPL. 2106110Reis, L.O., Fávaro, W.J., Ferreira, U., Billis, A., Fazuoli, M.G., Cagnon, V.H., Evolution on experimental animal model for upper urothelium carcinogenesis (2010) World J Urol, 28, pp. 499-505Mulder, G.J., Scholtens, E., Meijer, D.K., Collection of metabolites in bile and urine from the rat (1981) Methods Enzymol, 77, pp. 21-30Krinke, G.J., (2000) The Laboratory Rat, , San Diego, CA: Academic PressPhillips, J.I., Davies, I., The comparative morphology of the bladder and urethra in young and old female C57BL/Icrfat mice (1980) Exp Geront, 15, pp. 551-562Andersson, K.E., Arner, A., Urinary bladder contraction and relaxation: Physiology and pathophysiology (2004) Physiol Rev, 84, pp. 935-986Reis, L.O., Pereira, T.C., Favaro, W.J., Cagnon, V.H., Lopes-Cendes, I., Ferreira, U., Experimental animal model and RNA interference: A promising association for bladder cancer research (2009) World J Urol, 27, pp. 353-361Marini, R.P., Esteves, M.I., Fox, J.G., A technique for catheterization of the urinary bladder in the ferret (1994) Lab Anim, 28, pp. 155-15

Evaluation of semantic segmentation methods for deforestation detection in the amazon

Deforestation is a wide-reaching problem, responsible for serious environmental issues, such as biodiversity loss and global climate change. Containing approximately ten percent of all biomass on the planet and home to one tenth of the known species, the Amazon biome has faced important deforestation pressure in the last decades. Devising efficient deforestation detection methods is, therefore, key to combat illegal deforestation and to aid in the conception of public policies directed to promote sustainable development in the Amazon. In this work, we implement and evaluate a deforestation detection approach which is based on a Fully Convolutional, Deep Learning (DL) model: the DeepLabv3+. We compare the results obtained with the devised approach to those obtained with previously proposed DL-based methods (Early Fusion and Siamese Convolutional Network) using Landsat OLI-8 images acquired at different dates, covering a region of the Amazon forest. In order to evaluate the sensitivity of the methods to the amount of training data, we also evaluate them using varying training sample set sizes. The results show that all tested variants of the proposed method significantly outperform the other DL-based methods in terms of overall accuracy and F1-score. The gains in performance were even more substantial when limited amounts of samples were used in training the evaluated methods. © 2020 International Archives of the Photogrammetry, Remote Sensing and Spatial Information Sciences - ISPRS Archives

Dynamic Scaling in Diluted Systems Phase Transitions: Deactivation trough Thermal Dilution

Activated scaling is confirmed to hold in transverse field induced phase transitions of randomly diluted Ising systems. Quantum Monte Carlo calculations have been made not just at the percolation threshold but well bellow and above it including the Griffiths-McCoy phase. A novel deactivation phenomena in the Griffiths-McCoy phase is observed using a thermal (in contrast to random) dilution of the system.Comment: 4 pages, 4 figures, RevTe

Дискусійні питання історії ЗУНР

У статті аналізуються питання, які все ще залишаються в історіографії Західноукраїнської Народної Республіки. Автор звертає увагу на контроверсійні точки зору щодо революційних подій, які відбулися 1 листопада 1918 р. у Львові, стосовно часу існування ЗУНР, Акту злуки УНР та ЗУНР, причин поразки Української Галицької армії тощо.The author of the article analyses the questions that are still disputable in modern Ukrainian historiography. The author attracts our attention to the controversial points of view concerning the type of revolutionary events which took place on the 1 st of November 1918 in Lviv, concerning the time of WUPR existence, concerning the Act of Unification of Western Ukrainian People’s Republic end Ukrainian People’s Republic , concerning the reasons of the defeat of Ukrainian army, etc

Universal Critical Behavior of Aperiodic Ferromagnetic Models

We investigate the effects of geometric fluctuations, associated with aperiodic exchange interactions, on the critical behavior of $q$-state ferromagnetic Potts models on generalized diamond hierarchical lattices. For layered exchange interactions according to some two-letter substitutional sequences, and irrelevant geometric fluctuations, the exact recursion relations in parameter space display a non-trivial diagonal fixed point that governs the universal critical behavior. For relevant fluctuations, this fixed point becomes fully unstable, and we show the apperance of a two-cycle which is associated with a novel critical behavior. We use scaling arguments to calculate the critical exponent $\alpha$ of the specific heat, which turns out to be different from the value for the uniform case. We check the scaling predictions by a direct numerical analysis of the singularity of the thermodynamic free-energy. The agreement between scaling and direct calculations is excellent for stronger singularities (large values of $q$). The critical exponents do not depend on the strengths of the exchange interactions.Comment: 4 pages, 1 figure (included), RevTeX, submitted to Phys. Rev. E as a Rapid Communicatio

Decomposition and nutrient release of leguminous plants in coffee agroforestry systems.

Leguminous plants used as green manure are an important nutrient source for coffee plantations, especially for soils with low nutrient levels. Field experiments were conducted in the Zona da Mata of Minas Gerais State, Brazil to evaluate the decomposition and nutrient release rates of four leguminous species used as green manures (Arachis pintoi, Calopogonium mucunoides, Stizolobium aterrimum and Stylosanthes guianensis) in a coffee agroforestry system under two different climate conditions. The initial N contents in plant residues varied from 25.7 to 37.0 g kg-1 and P from 2.4 to 3.0 g kg-1. The lignin/N, lignin/polyphenol and(lignin+polyphenol)/N ratios were low in all residues studied. Mass loss rates were highest in the first 15 days, when 25 % of the residues were decomposed. From 15 to 30 days, the decomposition rate decreased on both farms. On the farm in Pedra Dourada (PD), the decomposition constant k increased in the order C. mucunoides < S. aterrimum < S. guianensis < A. pintoi. On the farm in Araponga (ARA), there was no difference in the decomposition rate among leguminous plants. The N release rates varied from 0.0036 to 0.0096 d-1. Around 32 % of the total N content in the plant material was released in the first 15 days. In ARA, the N concentration in the S. aterrimum residues was always significantly higher than in the other residues. At the end of 360 days, the N released was 78 % in ARA and 89 % in PD of the initial content. Phosphorus was the most rapidly released nutrient (k values from 0.0165 to 0.0394 d-1). Residue decomposition and nutrient release did not correlate with initial residue chemistry and biochemistry, but differences in climatic conditions between the two study sites modified the decomposition rate constants

On the critical behavior of disordered quantum magnets: The relevance of rare regions

The effects of quenched disorder on the critical properties of itinerant quantum antiferromagnets and ferromagnets are considered. Particular attention is paid to locally ordered spatial regions that are formed in the presence of quenched disorder even when the bulk system is still in the paramagnetic phase. These rare regions or local moments are reflected in the existence of spatially inhomogeneous saddle points of the Landau-Ginzburg-Wilson functional. We derive an effective theory that takes into account small fluctuations around all of these saddle points. The resulting free energy functional contains a new term in addition to those obtained within the conventional perturbative approach, and it comprises what would be considered non-perturbative effects within the latter. A renormalization group analysis shows that in the case of antiferromagnets, the previously found critical fixed point is unstable with respect to this new term, and that no stable critical fixed point exists at one-loop order. This is contrasted with the case of itinerant ferromagnets, where we find that the previously found critical behavior is unaffected by the rare regions due to an effective long-ranged interaction between the order parameter fluctuations.Comment: 16 pp., REVTeX, epsf, 2 figs, final version as publishe

Quantum Griffiths effects and smeared phase transitions in metals: theory and experiment

In this paper, we review theoretical and experimental research on rare region effects at quantum phase transitions in disordered itinerant electron systems. After summarizing a few basic concepts about phase transitions in the presence of quenched randomness, we introduce the idea of rare regions and discuss their importance. We then analyze in detail the different phenomena that can arise at magnetic quantum phase transitions in disordered metals, including quantum Griffiths singularities, smeared phase transitions, and cluster-glass formation. For each scenario, we discuss the resulting phase diagram and summarize the behavior of various observables. We then review several recent experiments that provide examples of these rare region phenomena. We conclude by discussing limitations of current approaches and open questions.Comment: 31 pages, 7 eps figures included, v2: discussion of the dissipative Ising chain fixed, references added, v3: final version as publishe