Načini upravljanja procesom fermentacije u siliranim hranivima

The control of fermentation process in ensilaged livestock feed is based on the knowledge of biomasses. The important aspects are the suitability for ensilaging (the content of fermentative carbohydrates and buffer capacity of the biomass), providing of the correct level of dry matter and anaerobic environment, wilting, using the chemical additives such as organic acids and their salts, using of biologic additives (inoculants), adsorption of mycotoxins, etc. In purpose of making the process of ensilaging of grasses, legumes and grass-legume mixes, the silages are prepared as bales or silo-tubes.Kontrola procesa fementacije bazira se na poznavanju biomasa u pogledu pogodnosti za siliranje, odnosno obezbeđivanju povoljnog odnosa šećera i pufernog kapaciteta. Biomase višegodišnjih leguminoza i trava neophodno je provenjavati, odnosno povećati sadržaj suve materije u silomasi na najmanje 300-400 g kg-1. Za teško silirajuće biomase (višegodišnje i jednogodišnje leguminoze) koristiti ugljenohidratna hraniva (kukuruzna prekrupa, prekrupa ostalih žitarica, suvi rezanci šećerne repe, melasa, i dr.). Korišćenje hemijskih sredstava iz reda organskih kiselina i njihovih soli. Upotreba bioloških dodataka, inokulanata u smeši sa enzimima (amilaze, celulaze, hemicelulaze i dr.) obezbeđuje dobijanje dobre i stabilne silaže, kao i silaže veće hranljive vrednosti. Korišćenje inokulanata sa homo i heterotrofnim mikroorganizmima u cilju obezbeđivanja aerobne stabilnosti silaža, posebno lakosilirajućih biomasa (kukuruza, sirka, sudanske trave, italijanskog ljulja i dr.)

Trendovi u siliranju leguminoza

Modern trends in legumes ensilaging technology are based on the knowledge of biomass from the aspect of suitability for ensilaging, wilting, addition of carbohydrate feed, use of biological additives, etc. Today, the experiments are conducted, worldwide, with inoculates, which, in addition to homofermentative, also contain heterofermentative lactic acid bacteria. Products of such inoculants contribute to the increase of aerobic stability of silages so their implementation is good for all types of silage. In addition to the usage of those additions, modern technology of silage is based on the maximum mechanization of the ensilaging process, as well as preparing the silage in the form of roto-bales and silo tubes (most inexpensive way of conservation) as well as permanent facilities.Savremeni trendovi u tehnologiji siliranja leguminoza zasnivaju se na poznavanju biomasa sa aspekta pogodnosti za siliranje, provenjavanju, dodavanju ugljenohidratnih hraniva, upotrebi bioloških dodataka, i dr. Trenutno se u svetu eksperimentiše sa inokulantima, koji pored homofermentativnih, sadrže i heterofermentativne bakterije mlečne kiseline. Produkti ovakvih inokulanata doprinose povećanju aerobne stabilnosti silaža, pa su navedeni dodaci aktuelni za sve vrste silaža. Osim korišćenja navedenih dodataka, savremena tehnologija siliranja se bazira na maksimalnoj mehanizovanosti celokupnog procesa siliranja, kao i pripremanja silaže u formi roto-bala i silokobasica (najeftiniji vid konzervisanja) kao i stalnih objekata

Recharging process of commercial floating-gate MOS transistor in dosimetry application

We investigated the recharging process of commercial floating gate device (EPAD) during the six different dose rates and ten irradiation cycles with the highest dose rate. Dose rate dependence of the floating gate dosimeter was observed from 1 Gy/h to 26 Gy/h (H2O). There is no change of the dosimetric characteristic with a constant dose rate of 26 Gy/h for ten cycles. The absorbed dose does not affect the drift of the threshold voltage readings after the irradiation steps. The reprogramming characteristic is not degrading with the absorbed dose for the ten irradiation cycles, giving the promising potential in the application for dosimetric purposes

CORRELATION OF MORPHOLOGIC AND PRODUCTION TRAITS OF HONEY BEE COLONIES FROM SERBIA

Abstract: State of mutual or reciprocal relationship between traits is determined by the correlation coefficients. Correlation of morphological and production traits was determined in two generations of honey bee colonies. In the first generation, mother queen bees were studied, and in the second generation, their offspring. The study of one generation lasted two years. Morphological traits were determined in the beginning of the study and production traits were determined in the spring and autumn inspections of every year. In the first generation, the positive and mostly high correlation between morphological traits, and honey yield and hygienic behaviour was determined. Correlation of morphological traits, colony strength and food supplies per colony ranged from highly positive to slightly negative values. It was found that there was a high correlation between some production traits (colony strength in the spring and autumn inspections and honey yield per colony). In the analysis of offspring (second generation), most traits that were positively correlated in mother generation now, also, showed a similar relationship. The only difference, in contrast to mother generation, was that, for most traits, the intensity of interaction increased

Gis techology in regional recognition of the distribution pattern of multifloral honey: the chemical traits in Serbia

GIS is a computer-based system to input, store, manipulate, analyze and output spatially referenced data. There is a huge range application of GIS that generally sets out to fulfill: mapping, measurement, monitoring, modeling and management. In this study, GIS technology was used for the regional recognition of origin and distribution patterns of multifloral honey chemical traits in Serbia. This included organizing and analyzing the spatial and attributive data of 164 honey samples collected from different regions of Serbia during the harvesting season of 2009. Multifloral honey was characterized in regards to mineral composition, sugar content and basic physicochemical properties. The kriging method of Geostatistical Analyst was used for interpolation to predict values of a sampled variable over the whole territory of Serbi

Multiferroic heterostructure BaTiO3/ε-Fe2O3composite obtained by in situ reaction

Solid-state reaction between BaTiO3and Fe2O3was used to produce a multiferroic heterostructure composite. Commercial BaTiO3and Fe(NO3)3•9H2O were suspended in ethanol for 30 minutes in an ultrasound bath. The prepared mixture was thermally processed at 300°C for 6 h. Sintering at 1300 °C for 1 h resulted in a mixture of different phases, BaTiO3, BaFe12O19and Ba12Ti28Fe15O84, which were confirmed by x-ray powder diffraction. A dense microstructure with a small volume fraction of closed porosity was indicated by the scanning electron microscopy, while a homogeneous distribution of Fe ions over BaTiO3phase was visible from energy dispersive spectroscopy mapping. Doping of BaTiO3with Fe2O3resulted in formation of magnetic hexaferrite phases, as confirmed by dielectric measurements that showed a broadened maximum of the permittivity measured as a function of temperature

Effect of mineral-enriched diet and medicinal herbs on Fe, Mn, Zn, and Cu uptake in chicken

<p>Abstract</p> <p>Background</p> <p>The goal of our study was to evaluate the effects of different medicinal herbs rich in polyphenol (Lemon balm, Sage, St. John's wort and Small-flowered Willowherb) used as dietary supplements on bioaccumulation of some essential metals (Fe, Mn, Zn and Cu) in different chicken meats (liver, legs and breast).</p> <p>Results</p> <p>In different type of chicken meats (liver, legs and breast) from chickens fed with diets enriched in minerals and medicinal herbs, beneficial metals (Fe, Mn, Zn and Cu) were analysed by flame atomic absorption spectrometry. Fe is the predominant metal in liver and Zn is the predominant metal in legs and breast chicken meats. The addition of metal salts in the feed influences the accumulations of all metals in the liver, legs and breast chicken meat with specific difference to the type of metal and meat. The greatest influences were observed in legs meat for Fe and Mn. Under the influence of polyphenol-rich medicinal herbs, accumulation of metals in the liver, legs and breast chicken meat presents specific differences for each medicinal herb, to the control group that received a diet supplemented with metal salts only. Great influence on all metal accumulation factors was observed in diet enriched with sage, which had significantly positive effect for all type of chicken meats.</p> <p>Conclusions</p> <p>Under the influence of medicinal herbs rich in different type of polyphenol, accumulation of metals in the liver, legs and breast chicken meat presents significant differences from the group that received a diet supplemented only with metal salts. Each medicinal herb from diet had a specific influence on the accumulation of metals and generally moderate or poor correlations were observed between total phenols and accumulation of metals. This may be due to antagonism between metal ions and presence of other chelating agents (amino acids and protein) from feeding diets which can act as competitor for complexation of metals and influence accumulation of metals in chicken meat.</p> <p><b>Graphical abstract</b></p

Dichrostachys cinerea (L.) Wight et Arn (Mimosaceae) hydro-alcoholic extract action on the contractility of tracheal smooth muscle isolated from guinea-pig

<p>Abstract</p> <p>Background</p> <p><it>Dichrostachys cinerea </it>(L.) Wight et Arn. (Mimosaceae) is largely used in ethno-medically across Africa, and mainly employed for the treatment of asthma in Ivory Coast and Gabon. The paper analyses the relaxation induced by the methanolic extract of <it>D. cinerea </it>(Edici) in the guinea-pig trachea preparations (GPTPs). Purpose: This study aimed to bring out the scientific basis to the use of this plant leading to the validation of this phytomedicine.</p> <p>Method</p> <p>The aorta obtained from guinea-pigs was immediately placed in a Mac Ewen solution. Experiments were performed in preparations suspended between two L-shaped stainless steel hooks in a 10 ml organ bath containing Mac Ewen solution. The isometric contractile force of the aorta strips of guinea-pig were recorded by using a strain gauge. The different drugs were directly administered into the organ bath and the magnitude of GPTPs was evaluated.</p> <p>Results</p> <p>Phytochemical analysis of the methanolic extract of Dichrostachys <it>cinerea </it>(Edici) using chemical methods revealed the presence of flavenoids, tannins, sterols, triterpenes and polyphenols. Pharmacological studies performed in GPTPs show that of <it>Dichrostachys cinerea </it>(0.1 mg/ml - 2 mg/ml) evoked a broncho-constriction in GPTPs. Whereas, at concentration up to 2 mg/ml, Edici induced a significant dose-dependent relaxation in the GPTPs. KCl-, ACh- or histamine-evoked contractions of isolated trachea was significantly inhibited by increasing concentrations of Edici (3.5-10 mg/ml). Edici (10 mg/ml) as well as promethazine (0.25 mg/ml) significantly inhibited contractions induced by increasing concentrations of histamine (1×10^-7-1×10^-4mg/ml). In the presence of atropine at a concentration of 10^-6mg/ml, contractile response curve (CRC) evoked by ACh (1×10^-5-1×10^-2mg/ml) was significantly abolished in concentration-dependent manner. Edici did not significantly reduced ACh evoked contraction (10^-5-10^-2mg/ml).</p> <p>Conclusion</p> <p>These observations suggest that Edici could act through two mechanisms: firstly by activation of β-adrenergic or histaminergic receptors; and secondly muscarinic receptors may not be greatly involved, that justifying the use of the extract in traditional Medicine in Africa.</p

Akt-Induced Phosphorylation of N-CoR at Serine 1450 Contributes to Its Misfolded Conformational Dependent Loss (MCDL) in Acute Myeloid Leukemia of the M5 Subtype

10.1371/journal.pone.0070891PLoS ONE88-POLN

Mdm20 Stimulates PolyQ Aggregation via Inhibiting Autophagy Through Akt-Ser473 Phosphorylation

Mdm20 is an auxiliary subunit of the NatB complex, which includes Nat5, the catalytic subunit for protein N-terminal acetylation. The NatB complex catalyzes N-acetylation during de novo protein synthesis initiation; however, recent evidence from yeast suggests that NatB also affects post-translational modification of tropomyosin, which is involved in intracellular sorting of aggregated proteins. We hypothesized that an acetylation complex such as NatB may contribute to protein clearance and/or proteostasis in mammalian cells. Using a poly glutamine (polyQ) aggregation system, we examined whether the NatB complex or its components affect protein aggregation in rat primary cultured hippocampal neurons and HEK293 cells. The number of polyQ aggregates increased in Mdm20 over-expressing (OE) cells, but not in Nat5-OE cells. Conversely, in Mdm20 knockdown (KD) cells, but not in Nat5-KD cells, polyQ aggregation was significantly reduced. Although Mdm20 directly associates with Nat5, the overall cellular localization of the two proteins was slightly distinct, and Mdm20 apparently co-localized with the polyQ aggregates. Furthermore, in Mdm20-KD cells, a punctate appearance of LC3 was evident, suggesting the induction of autophagy. Consistent with this notion, phosphorylation of Akt, most notably at Ser473, was greatly reduced in Mdm20-KD cells. These results demonstrate that Mdm20, the so-called auxiliary subunit of the translation-coupled protein N-acetylation complex, contributes to protein clearance and/or aggregate formation by affecting the phosphorylation level of Akt indepenently from the function of Nat5