Toward the Development of Load Transfer Efficiency Evaluation of Rigid Pavements by a Rolling Wheel Deflectometer

The jointed rigid pavement is currently evaluated by the Falling weight deflectometer which is rather slow for the testing of the jointed pavements. Continuous nondestructive evaluation of rigid pavements with a rolling wheel deflectometer can be used to measure the load transfer and is investigated. Load transfer is an important indicator of the rigid pavement's condition and this is the primary factor which is studied. Continuous data from experimental measurements across a joint allows for the determination of not only the load transfer efficiency provided parameters characterizing the pavement is known. A three-dimensional semi-analytical model was implemented for simulating the pavement response near a joint and used for interpretation and verification of the experimental data. Results show that this development is promising for the use of a rolling wheel deflectometer for rapid evaluation of joints

Evaluation of Load transfer in rigid pavements by Rolling wheel deflectometer and Falling weight deflectometer

Rigid pavements have widespread use, e.g, in motorways and airports, due to their excellent properties such as high bearing capacity and long lifetime. However, when rigid pavements fail it is often due to bad load transfer efficiency (LTE) at its joints. Traditional methods of measuring LTE can be time consuming. Here, we study the possibility of measuring LTE using a moving load with the aim of achieving higher productivity. An experiment simulating Rolling Weight Deflectometer (RWD) measurements on a joint was carried out to gain understanding and confidence that can guide the analysis of real RWD data. Continuous data from measurements across a joint allows for determination of not only the LTE but also additional parameters characterizing the pavement and the joint. A semi-analytical model was implemented for simulating the pavement response next to a joint and used for interpretation and verification of the experimental data. The results show promise for the use of moving loads for rapid evaluation of joints

Homology Modeling and Molecular Docking for the Science Curriculum

DockoMatic 2.0 is a powerful open source software program (downloadable from sourceforge.net) that allows users to utilize a readily accessible computational tool to explore biomolecules and their interactions. This manuscript describes a practical tutorial for use in the undergraduate curriculum that introduces students to macromolecular structure creation, ligand binding calculations, and visualization of docking results. A student procedure is provided that illustrates the use of DockoMatic to create a homology model for the amino propeptide region (223 amino acids with two disulfide bonds) of collagen α1 (XI), followed by molecular docking of the commercial drug Arixtra® to the homology model of α1 (XI), and finally, analysis of the results of the docking experiment. The activities and Supporting Information described are intended to educate students in the use of computational tools to create and investigate homology models for other systems of interest and to train students to perform and analyze molecular docking studies. The tutorial also serves as a foundation for investigators seeking to explore the viability of using computational biochemistry to study their receptor–ligand binding motifs

A Coastal Retracking System for Satellite Radar Altimeter Waveforms: Application to ERS-2 Around Australia

Satellite radar altimeter-derived sea surface heights (SSH) are in error in coastal regions due, in part, to the complex nature of echoes returned from rapidly varying land and sea surfaces. This paper presents improved altimeter-derived SSH results in Australian coastal regions using the waveform retracking technique, which reprocesses the waveform data through a “coastal retracking system”. The system, based upon a systematic analysis of satellite radar altimeter waveforms around Australia, improves SSH data from several retrackers depending on the waveforms' characteristics. Central to the system is the use of two techniques: the least squares fitting and the threshold retracking algorithms. To overcome the problem of fading noise, the fitting algorithm has been developed to include a weighted iterative scheme. The retrackers include five fitting models and the threshold method with varying threshold levels. A waveform classification procedure has also been developed, which enables the waveforms to be sorted and then retracked by an appropriate retracker. Two cycles of 20-Hz waveform data from ERS-2 have been reprocessed using this system to obtain the improved SSH estimates. Using the AUSGeoid98 gravimetric geoid model as a quasi-independent reference, the system improves SSH estimates from beyond ∼22 km to beyond ∼5 km from the coastline

The HyVac4 Subunit Vaccine Efficiently Boosts BCG-Primed Anti-Mycobacterial Protective Immunity

BACKGROUND: The current vaccine against tuberculosis (TB), BCG, has failed to control TB worldwide and the protective efficacy is moreover limited to 10-15 years. A vaccine that could efficiently boost a BCG-induced immune response and thus prolong protective immunity would therefore have a significant impact on the global TB-burden. METHODS/FINDINGS: In the present study we show that the fusion protein HyVac4 (H4), consisting of the mycobacterial antigens Ag85B and TB10.4, given in the adjuvant IC31® or DDA/MPL effectively boosted and prolonged immunity induced by BCG, leading to improved protection against infection with virulent M. tuberculosis (M.tb). Increased protection correlated with an increased percentage of TB10.4 specific IFNγ/TNFα/IL-2 or TNFα/IL-2 producing CD4 T cells at the site of infection. Moreover, this vaccine strategy did not compromise the use of ESAT-6 as an accurate correlate of disease development/vaccine efficacy. Indeed both CD4 and CD8 ESAT-6 specific T cells showed significant correlation with bacterial levels. CONCLUSIONS/SIGNIFICANCE: H4-IC31® can efficiently boost BCG-primed immunity leading to an increased protective anti-M.tb immune response dominated by IFNγ/TNFα/IL-2 or TNFα/IL2 producing CD4 T cells. H4 in the CD4 T cell inducing adjuvant IC31® is presently in clinical trials

Hippocampal involvement in nonpathological déjà vu: Subfield vulnerability rather than temporal lobe epilepsy equivalent

Introduction Morphological correlates of nonpathological déjà vu (DV) have been identified recently within the human brain. Significantly reduced gray matter volume (GMV) within a set of cortical and subcortical regions reported in subjects experiencing DV seems to mirror the distribution of GMV reduction in mesial temporal lobe epilepsy (MTLE) patients but vary in terms of the hippocampus. Another condition associated with hippocampal GMV reduction and DV alike disturbance in memory processing is schizophrenia (SCH). Here, we tested the hypothesis that hippocampal involvement in nonpathological DV resembles more closely the pattern of GMV decrease observed in MTLE compared with that occurring in SCH. Methods Using automated segmentation of the MRI data we compared the medians of GMV within 12 specific hippocampal subfields in healthy individuals that do (DV+; N = 87) and do not report déjà vu experience (DV−; N = 26), and patients with MTLE (N = 47) and SCH (N = 29). By Pearson correlation, we then evaluated the similarity of MTLE and SCH groups to DV+ group with respect to spatial distribution of GMV deviation from DV− group. Results Significant GMV decrease was found in MTLE group in most of the subfields. There were just trends in the hippocampal GMV decrease found in DV+ or SCH groups. Concerning the spatial distribution of GMV decrease, we revealed statistically significant correlation for the left hippocampus for SCH vs DV+. Otherwise there was no statistically significant correlation. Conclusions Our findings reveal structural features of hippocampal involvement in nonpathological DV, MTLE, and SCH. Despite our expectations, the pattern of GMV reduction in the DV+ relative to the DV− group does not resemble the pattern observed in MTLE any more than that observed in SCH. The highly similar patterns of the three clinical groups rather suggest an increased vulnerability of certain hippocampal subfields; namely, Cornu Ammonis (CA)4, CA3, dentate gyrus granular cell layer (GC‐DG), hippocampal–amygdaloid transition area (HATA) and subiculum

Uptake of health services for common mental disorders by first-generation Turkish and Moroccan migrants in the Netherlands

Abstract Background Migration and ethnic minority status have been associated with higher occurrence of common mental disorders (CMD), while mental health care utilisation by non-Western migrants has been reported to be low compared to the general population in Western host countries. Still, the evidence-base for this is poor. This study evaluates uptake of mental health services for CMD and psychological distress among first-generation non-Western migrants in Amsterdam, the Netherlands. Methods A population-based survey. First generation non-Western migrants and ethnic Dutch respondents (N = 580) participated in structured interviews in their own languages. The interview included the Composite International Diagnostic Interview (CIDI) and the Kessler psychological distress scale (K10). Uptake of services was measured by self-report. Data were analysed using weighting techniques and multivariate logistic regression. Results Of subjects with a CMD during six months preceding the interview, 50.9% reported care for mental problems in that period; 35.0% contacted specialised services. In relation to CMD, ethnic groups were equally likely to access specialised mental health services. In relation to psychological distress, however, Moroccan migrants reported less uptake of primary care services (OR = 0.37; 95% CI = 0.15 to 0.88). Conclusion About half of the ethnic Dutch, Turkish and Moroccan population in Amsterdam with CMD contact mental health services. Since the primary purpose of specialised mental health services is to treat "cases", this study provides strong indications for equal access to specialised care for these ethnic groups. The purpose of primary care services is however to treat psychological distress, so that access appears to be lower among Moroccan migrants

Light dependence of Zostera marina annual growth dynamics in estuaries subject to different degrees of eutrophication

Author Posting. © The Authors, 2005. This is the author's version of the work. It is posted here by permission of Elsevier B. V. for personal use, not for redistribution. The definitive version was published in Aquatic Botany 84 (2006): 17-25, doi:10.1016/j.aquabot.2005.05.014.In temperate, shallow systems with clear waters the temporal dynamic of eelgrass (Zostera marina) growth is closely associated with the seasonality of irradiance at the water's surface. It has been recently suggested that increasing eutrophication, via light attenuation by increased algal growth, may disrupt the close temporal association between eelgrass growth and surface irradiance often found in pristine sites. Here, we test this hypothesis by examining the coupling between eelgrass growth dynamics and surface irradiance over an annual cycle in four shallow estuaries of the Waquoit Bay system (Massachusetts, USA) that have similar physical characteristics, but are subject to different land-derived nitrogen loading rates and the intensity of eutrophication sustained. Contrary to our hypothesis, the results show that, in general, most measures of eelgrass demographics were positively correlated with surface irradiance in all four estuaries. Out of the 45 regression models adjusted between irradiance and demographic variables (density, plastochrone intervals, and above- or below-ground biomass, growth, and production, on both a per shoot and areal basis), only 9 of them were non-significant, and only 6 of those corresponded to the eutrophic estuaries. Most notably, we found a lack of correlation between shoot density and irradiance in the eutrophic estuaries, in contrast to the strong coupling exhibited in estuaries receiving the lowest nitrogen loads. Experimental evidence from previous work has demonstrated severe light limitation and other deleterious impacts imposed by macroalgal canopies on newly recruiting shoots in the eutrophic estuaries, likely contributing to the lack of correlation between shoot density and irradiance at the water's surface. Because the range in eutrophication encompassed by this comparison includes the range of conditions at which eelgrass can survive, the relatively consistent temporal coupling between surface irradiance and most eelgrass demographic variables found here may also be a feature of other shallow temperate systems undergoing increasing eutrophication, and indicates a measure of plant recruitment (density) to be one of the first parameters to become uncoupled from light reaching the water's surface.This research was supported by an Environmental Protection Agency STAR Fellowship for Graduate Environmental Study (U-915335-01-0) and a National Estuarine Research Reserve Graduate Research Fellowship from the National Oceanic and Atmospheric Administration (award number NA77OR0228) awarded to JH. We thank the Quebec-Labrador Foundation Atlantic Center for the Environment’s Sounds Conservancy Program and the Boston University Ablon/Bay committee for research funds

Investigating Homology between Proteins using Energetic Profiles

Accumulated experimental observations demonstrate that protein stability is often preserved upon conservative point mutation. In contrast, less is known about the effects of large sequence or structure changes on the stability of a particular fold. Almost completely unknown is the degree to which stability of different regions of a protein is generally preserved throughout evolution. In this work, these questions are addressed through thermodynamic analysis of a large representative sample of protein fold space based on remote, yet accepted, homology. More than 3,000 proteins were computationally analyzed using the structural-thermodynamic algorithm COREX/BEST. Estimated position-specific stability (i.e., local Gibbs free energy of folding) and its component enthalpy and entropy were quantitatively compared between all proteins in the sample according to all-vs.-all pairwise structural alignment. It was discovered that the local stabilities of homologous pairs were significantly more correlated than those of non-homologous pairs, indicating that local stability was indeed generally conserved throughout evolution. However, the position-specific enthalpy and entropy underlying stability were less correlated, suggesting that the overall regional stability of a protein was more important than the thermodynamic mechanism utilized to achieve that stability. Finally, two different types of statistically exceptional evolutionary structure-thermodynamic relationships were noted. First, many homologous proteins contained regions of similar thermodynamics despite localized structure change, suggesting a thermodynamic mechanism enabling evolutionary fold change. Second, some homologous proteins with extremely similar structures nonetheless exhibited different local stabilities, a phenomenon previously observed experimentally in this laboratory. These two observations, in conjunction with the principal conclusion that homologous proteins generally conserved local stability, may provide guidance for a future thermodynamically informed classification of protein homology