Lettuce yield response to application of rhizobacteria and nitrogen to the growth substrate

Rhizobacteria of agronomic interest can promote plant growth, increase productivity, and reduce the demand for nitrogen (N) fertilizers by improving the N use efficiency in crops. The objective of this study was to assess the effects of rhizobacteria and nitrogen on the yield of lettuce plants (cultivar Vera). The experiment was carried out in a greenhouse, using a completely randomized design in a 2×2×2×2 factorial arrangement, with five repetitions. The factors consisted of single and combined applications of liquid inoculants (1 mL) based on Azospirillum brasilense, Bacillus subtilis, and Bradyrhizobium japonicum, as well as nitrogen (75 kg ha-1) in the growth substrate. The variables assessed were total and commercial shoot fresh and dry weights, root dry weight, total dry weight, and numbers of total and commercial leaves, and shoot nitrogen content. Lettuce yield components improved with nitrogen fertilizer application. The single application of rhizobacteria did not increase lettuce yield, as the plants had decreases in root weight in response to Bradyrhizobium japonicum and decreases in total and commercial shoot dry weights, root dry weight, and total dry weight when Bacillus subtilis was used. However, the combined application of Azospirillum brasilense, Bacillus subtilis, and nitrogen increased the lettuce number of commercial leaves and shoot nitrogen content when one or another microbial specie was associated with nitrogen fertilizer.Rhizobacteria of agronomic interest can promote plant growth, increase productivity, and reduce the demand for nitrogen (N) fertilizers by improving the N use efficiency in crops. The objective of this study was to assess the effects of rhizobacteria and nitrogen on the yield of lettuce plants (cultivar Vera). The experiment was carried out in a greenhouse, using a completely randomized design in a 2×2×2×2 factorial arrangement, with five repetitions. The factors consisted of single and combined applications of liquid inoculants (1 mL) based on Azospirillum brasilense, Bacillus subtilis, and Bradyrhizobium japonicum, as well as nitrogen (75 kg ha-1) in the growth substrate. The variables assessed were total and commercial shoot fresh and dry weights, root dry weight, total dry weight, and numbers of total and commercial leaves, and shoot nitrogen content. Lettuce yield components improved with nitrogen fertilizer application. The single application of rhizobacteria did not increase lettuce yield, as the plants had decreases in root weight in response to Bradyrhizobium japonicum and decreases in total and commercial shoot dry weights, root dry weight, and total dry weight when Bacillus subtilis was used. However, the combined application of Azospirillum brasilense, Bacillus subtilis, and nitrogen increased the lettuce number of commercial leaves and shoot nitrogen content when one or another microbial specie was associated with nitrogen fertilizer

ANÁLISE HISTOLÓGICA, HISTOQUÍMICA E ULTRAESTRUTURAL DA MUSCULATURA DORSAL E VENTRAL DO GUARU (Poecilia vivipara BLOCHI & SCHNEIDER, 1801)

Poecilia vivipara (guaru), peixe neotropical de pequeno porte, eurialino, tem sido usado como biomonitor. Analisou-se a musculatura dorsal e ventral do guaru, a fim de se padronizar metodologias para avaliação da estrutura normal deste tecido, com a perspectiva de que sejam usados em estudos de toxicidade. Para tanto, realizaram-se fixações químicas e físicas, que visaram procedimentos histológicos e histoquímicos de coloração e reação. Os métodos H.E. e TM evidenciaram a organização das FMEE, com feixes musculares epiaxial e hipaxial e conjuntivo associado. O AM marcou as FMEE e facilitou a observação dos sarcômeros com a presença de uma fina faixa corada metacromaticamente. Parte das FMEE foi PAS positiva e amilase reativa, indicando a distribuição de glicogênio entre as fibras. Na presença do SBB, as fibras foram reativas, principalmente na região dorsal, indicando a presença de lipídeos. Na SDH pH 10,5 detectou-se presença de células com citoplasma rico em enzimas mitocondriais. As FMEE coradas com AT apresentaram-se organizadas em faixas transversais alternadas, que correspondem ao padrão visualizado ao MET. O uso destas metodologias permitiu indicar a musculatura do guaru como um instrumento para estudos de variações ambientais, visto que sua estrutura normal é padrão nos teleósteos. PALAVRAS-CHAVES: Fibra muscular, morfologia, peixe neotropical

Experiência de uma consulta de cardio-oncologia num hospital universitário terciário em Portugal: estudo observacional

© 2022 Sociedade Portuguesa de Cardiologia. Published by Elsevier Espa ̃na, S.L.U. This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/)Introduction: Heart disease and cancer are the two leading causes of morbidity and mortality worldwide. Advances in cancer screening and management have led to longer survival and better quality of life. Despite this progress, many cancer patients experience cardiovascular complications during and after cancer treatment. This study describes the experience of a cardio-oncology program at tertiary academic hospital. Methods: In this retrospective observational study, cancer patients referred to the CHULN cardio-oncology consultation (COC) between January 2016 and December of 2019 were included. Data collected included: patient demographics, cancer type, reason for referral, cardiovascular risk factors, cardiac and oncologic treatments and clinical outcomes. Results: A total of 520 patients (mean age: 65 ± 14 years; 65% women) were referred to the COC. The main reasons for referral were suspected heart failure (26%), pre-high risk chemotherapy assessment (20%) and decreased LVEF (15%). Pre-existing cardiovascular risk factors were common (79%) and 309 (59%) were taking cardiac medications. The most common type of malignancy was breast cancer (216, 41%) followed by gastrointestinal (139, 27%). More than half received anthracycline-based regimens (303, 58%). Most patients (401; 77%) successfully completed cancer therapy. At the time of last data collection, the majority of patients were alive (430, 83%). Cardiac-related mortality was observed in 16%. Conclusions: The close collaboration between cardiology and oncology teams and timely cardiac monitoring was the key to the majority of patients to completing their prescribed cancer therapy.info:eu-repo/semantics/publishedVersio

DESEMPENHO COGNITIVO DE PRÉ-ESCOLARES COM BAIXA ESTATURA EM TRATAMENTO DE RECUPERAÇÃO NUTRICIONAL

RESUMO Objetivo: Determinar se o tratamento de crianças com baixa estatura, ofertado em um centro especializado, influencia seu desempenho cognitivo. Métodos: Foram analisados dois grupos de crianças advindas de famílias vulneráveis, um com crianças com baixa estatura em tratamento no Centro de Recuperação e Educação Nutricional (CREN) e outro de crianças eutróficas de uma creche municipal localizada na mesma região do CREN. No CREN, as crianças são tratadas em semi-internato (9 horas/dia, 5 dias/semana), recebendo suporte médico, nutricional e psicopedagógico. Todas foram submetidas ao Teste de Triagem do Desenvolvimento de Denver-II e avaliadas quanto ao índice de estatura-para-idade em 3 momentos distintos: no início do acompanhamento e após 6 e 12 meses. A classificação socioeconômica das crianças, de acordo com os Critérios de Classificação Econômica Brasil, foi feita no início do acompanhamento. Calcularam-se razões de prevalência para a análise transversal da linha de base, por meio de uma regressão de Poisson, e razões de prevalência agrupadas para a análise longitudinal, por meio de um modelo de estimativas de equações generalizadas, ambas ajustadas por idade, sexo e classe socioeconômica. Resultados: Ao todo, 74 crianças foram analisadas, 37 em cada grupo. Não houve diferenças de idade, sexo e classe socioeconômica entre os grupos. Na análise longitudinal, o grupo CREN apresentou melhor desempenho no domínio pessoal-social (razão de prevalência agrupada: 0,89; intervalo de confiança de 95% - IC95%: 0,82-0,95), sem diferenças significativas para os demais domínios. Conclusão: O tratamento ofertado pelo CREN melhorou satisfatoriamente as habilidades sociais das crianças tratadas, sem alterar os demais domínios

Patient-physician discordance in assessment of adherence to inhaled controller medication: a cross-sectional analysis of two cohorts

We aimed to compare patient's and physician's ratings of inhaled medication adherence and to identify predictors of patient-physician discordance.(SFRH/BPD/115169/2016) funded by Fundação para a Ciência e Tecnologia (FCT); ERDF (European Regional Development Fund) through the operations: POCI-01-0145-FEDER-029130 ('mINSPIRERS—mHealth to measure and improve adherence to medication in chronic obstructive respiratory diseases—generalisation and evaluation of gamification, peer support and advanced image processing technologies') cofunded by the COMPETE2020 (Programa Operacional Competitividade e Internacionalização), Portugal 2020 and by Portuguese Funds through FCT (Fundação para a Ciência e a Tecnologia).info:eu-repo/semantics/publishedVersio

Identification of clusters of asthma control: A preliminary analysis of the inspirers studies

This work was funded by ERDF (European Regional Development Fund) through the operations: POCI- -01-0145-FEDER-029130 (“mINSPIRERS—mHealth to measure and improve adherence to medication in chronic obstructive respiratory diseases - generalisation and evaluation of gamification, peer support and advanced image processing technologies”) co-funded by the COMPETE2020 (Programa Operacional Competitividade e Internacionalização), Portugal 2020 and by Portuguese Funds through FCT (Fundação para a Ciência e a Tecnologia).© 2020, Sociedade Portuguesa de Alergologia e Imunologia Clinica. All rights reserved. Aims: To identify distinct asthma control clusters based on Control of Allergic Rhinitis and Asthma Test (CARAT) and to compare patients’ characteristics among these clusters. Methods: Adults and adolescents (≥13 years) with persistent asthma were recruited at 29 Portuguese hospital outpatient clinics, in the context of two observational studies of the INSPIRERS project. Demographic and clinical characteristics, adherence to inhaled medication, beliefs about inhaled medication, anxiety and depression, quality of life, and asthma control (CARAT, >24 good control) were collected. Hierarchical cluster analysis was performed using CARAT total score (CARAT-T). Results: 410 patients (68% adults), with a median (percentile 25–percentile 75) age of 28 (16-46) years, were analysed. Three clusters were identified [mean CARAT-T (min-max)]: cluster 1 [27(24-30)], cluster 2 [19(14-23)] and cluster 3 [10(2-13)]. Patients in cluster 1 (34%) were characterised by better asthma control, better quality of life, higher inhaler adherence and use of a single inhaler. Patients in clusters 2 (50%) and 3 (16%) had uncontrolled asthma, lower inhaler adherence, more symptoms of anxiety and depression and more than half had at least one exacerbation in the previous year. Further-more, patients in cluster 3 were predominantly female, had more unscheduled medical visits and more anxiety symp-toms, perceived a higher necessity of their prescribed inhalers but also higher levels of concern about taking these inhalers. There were no differences in age, body mass index, lung function, smoking status, hospital admissions or specialist physician follow-up time among the three clusters. Conclusion: An unsupervised method based on CARAT--T, identified 3 clusters of patients with distinct, clinically meaningful characteristics. The cluster with better asthma control had a cut-off similar to the established in the validation study of CARAT and an additional cut-off seems to distinguish more severe disease. Further research is necessary to validate the asthma control clusters identified.publishersversionpublishe

MULTILINGUISMO DO ESTADO DO MARANHÃO: A DINÂMICA DA LIBRAS, LÍNGUA DE SINAIS KAAPOR E LÍNGUA PORTUGUESA NO DIALETO MARANHENSE

Santos et al. (2020) in their study observe that the Maranhense dialect is unique throughout the national territory, it has its linguistic vices, but it is still possible to flex verbs and use the pronouns in their correct form. With regard to Libras (Brazilian Sign Language), they describe that there is a diversification of signs in their own elaboration and transfer of emotions in all aspects, this is a characteristic of this State. About the Ka'apor tribe, what is known is that they appeared in the region bathed by the Xingu and Tocantins rivers, 300 years ago. The main objective of this work is to present the multilingualism present in the state of Maranhão, addressing dialects, accents, emergence, variations, among other aspects, and to materialize the linguistics of Maranhão and its importance in enhancing bilingualism and indigenous sign languages ​​within classrooms. class based on public policies for inclusive education, cultural and educational diversity. This work consists of a narrative and descriptive bibliographic investigation, which is carried out by consulting research databases such as the Scientific Electronic Library Online (SCIELO), the Education Resources Information Center (ERIC) and the academic Google. The study brought important reflections on the relevance of the languages ​​present in the state of Maranhão, such as the oral Portuguese language and the signed languages ​​of Libras and the Brazilian Kaapor Sign Language (LSKB), as well as characterizing the multifaceted linguistics present in the state and their relevance in valuing bilingualism and indigenous sign language.Santos et al. (2020) en su estudio señalan que el dialecto Maranhão es único en todo el país, tiene sus vicios lingüísticos, pero los verbos aún pueden ser flexionados y los pronombres en su forma correcta. Con respecto a libras (lengua de señas brasileña), describen que hay una diversificación de signos en su propia elaboración y transferencia de emociones en todos los aspectos, esto es característico de este Estado. Sobre la tribu Kaapor, lo que sí se sabe es que tuvieron su surgimiento en la región bañada por los ríos Xingu y Tocantins, hace 300 años. El objetivo principal de este trabajo es presentar el multilingüismo presente en el estado de Maranhão, abordando dialectos, acentos, emergencias, variaciones, entre otros aspectos y realizando la lingüística de Maranhão y su importancia en la valoración del bilingüismo y las lenguas de signos indígenas dentro de las aulas basadas en las políticas públicas de educación inclusiva, diversidad cultural y educativa. Este trabajo consiste en una investigación bibliográfica narrativa y descriptiva, que se realiza a través de la consulta en bases de datos de investigación como la Biblioteca Científica Electrónica en Línea (SCIELO), el Centro de Información de Recursos Educativos (ERIC) y el académico Google. El estudio trajo importantes reflexiones sobre la relevancia de las lenguas presentes en el estado de Maranhão, como la lengua oral portuguesa y las lenguas de señas de Libras y la Lengua de Señas Ka'apor Brasileira (LSKB), así como caracterizó los multifacéticos lingüísticos presentes.Santos e colaboradores (2020) em seu estudo observam que o dialeto maranhense é único em todo território nacional, possui seus vícios linguísticos, porém ainda se consegue flexionar verbos e empregar os pronomes em sua forma correta. No que se refere a Libras (Língua Brasileira de Sinais), descrevem que acontece uma diversificação de sinais na sua elaboração própria e repasse de emoções em todos os aspectos, isso é característica própria deste Estado. Acerca da tribo Kaapor, o que se sabe é que tiveram seu surgimento na região banhada pelos rios Xingu e Tocantins, há 300 anos. O objetivo principal deste trabalho é apresentar o multilinguismo presente no estado do Maranhão, abordando dialetos, sotaques, surgimento, variações, entre outros aspectos e concretizar a linguística do Maranhão e sua importância em valorizar o bilinguismo e as línguas de sinais indígenas dentro de salas de aula tendo como base as políticas públicas da educação inclusiva, diversidade cultural e educacional. Este trabalho consiste em uma investigação bibliográfica narrativa e descritiva, sendo esta realizada através de consulta em bases de dados de pesquisa como a Scientific Electronic Library Online (SCIELO), o Education Resources Information Center (ERIC) e o Google acadêmico. O estudo trouxe reflexões importantes sobre a relevância das línguas presentes no estado do Maranhão como a Língua Portuguesa oral e as línguas sinalizadas de Libras e a Língua de Sinais Ka’apor Brasileira (LSKB), bem como também caracterizou as multifacetas linguísticas presentes no Estado e sua relevância em valorizar o bilinguismo e a língua de sinais indígena.Santos e colaboradores (2020) em seu estudo observam que o dialeto maranhense é único em todo território nacional, possui seus vícios linguísticos, porém ainda se consegue flexionar verbos e empregar os pronomes em sua forma correta. No que se refere a Libras (Língua Brasileira de Sinais), descrevem que acontece uma diversificação de sinais na sua elaboração própria e repasse de emoções em todos os aspectos, isso é característica própria deste Estado. Acerca da tribo Kaapor, o que se sabe é que tiveram seu surgimento na região banhada pelos rios Xingu e Tocantins, há 300 anos. O objetivo principal deste trabalho é apresentar o multilinguismo presente no estado do Maranhão, abordando dialetos, sotaques, surgimento, variações, entre outros aspectos e concretizar a linguística do Maranhão e sua importância em valorizar o bilinguismo e as línguas de sinais indígenas dentro de salas de aula tendo como base as políticas públicas da educação inclusiva, diversidade cultural e educacional. Este trabalho consiste em uma investigação bibliográfica narrativa e descritiva, sendo esta realizada através de consulta em bases de dados de pesquisa como a Scientific Electronic Library Online (SCIELO), o Education Resources Information Center (ERIC) e o Google acadêmico. O estudo trouxe reflexões importantes sobre a relevância das línguas presentes no estado do Maranhão como a Língua Portuguesa oral e as línguas sinalizadas de Libras e a Língua de Sinais Ka’apor Brasileira (LSKB), bem como também caracterizou as multifacetas linguísticas presentes no Estado e sua relevância em valorizar o bilinguismo e a língua de sinais indígena

Pervasive gaps in Amazonian ecological research

Biodiversity loss is one of the main challenges of our time,1,2 and attempts to address it require a clear un derstanding of how ecological communities respond to environmental change across time and space.3,4 While the increasing availability of global databases on ecological communities has advanced our knowledge of biodiversity sensitivity to environmental changes,5–7 vast areas of the tropics remain understudied.8–11 In the American tropics, Amazonia stands out as the world’s most diverse rainforest and the primary source of Neotropical biodiversity,12 but it remains among the least known forests in America and is often underrepre sented in biodiversity databases.13–15 To worsen this situation, human-induced modifications16,17 may elim inate pieces of the Amazon’s biodiversity puzzle before we can use them to understand how ecological com munities are responding. To increase generalization and applicability of biodiversity knowledge,18,19 it is thus crucial to reduce biases in ecological research, particularly in regions projected to face the most pronounced environmental changes. We integrate ecological community metadata of 7,694 sampling sites for multiple or ganism groups in a machine learning model framework to map the research probability across the Brazilian Amazonia, while identifying the region’s vulnerability to environmental change. 15%–18% of the most ne glected areas in ecological research are expected to experience severe climate or land use changes by 2050. This means that unless we take immediate action, we will not be able to establish their current status, much less monitor how it is changing and what is being lostinfo:eu-repo/semantics/publishedVersio