Upregulation of tRNA-Ser-AGA-2-1 promotes malignant behavior in normal bronchial cells

Serine tRNAs (tRNASer) are frequently overexpressed in tumors and associated with poor prognosis and increased risk of recurrence in breast cancer. Impairment of tRNA biogenesis and abundance also impacts proteome homeostasis, and activates protein quality control systems. Herein, we aimed at testing whether increasing tRNASer abundance could foster tumor establishment through activation of the UPR. In order to do so, firstly we confirmed that the expression of tRNA-Ser-AGA-2-1 [hereafter tRNASer(AGA)] was upregulated by 1.79-fold in Stage I NSCLC tumors when compared to normal adjacent tissue. To study the impact of tRNASer(AGA) in early stage tumorigenesis, we induced its upregulation in a non-tumoral bronchial cell line, BEAS-2B. Upregulation of this tRNA increased cellular proliferation and protein synthesis rate, driven by eIF2α dephosphorylation and ATF4 activation downstream of PERK signaling. Futhermore, tRNASer(AGA) enhanced transformation potential in vitro, and promoted the establishment of slow growing tumors with aggressive features in nude mice. Our work highlights the importance of studying tRNA deregulation on early stage tumorigenesis, as they may be potential malignancy and aggressiveness biomarkers.publishe

Project Hand in Hand

The project “Parents and Teachers Working Hand in Hand : Training Programme for Parents and Teachers of Pupils with Attention Deficit Hyperactivity Disorder (ADHD)” was prepared and co-ordinated by Ankara Provincial Directorate for National Education under the European Union Education and Youth Programme, Lifelong Learning Programme within the framework of Grundtvig Learning Partnership. At the planning stage of the project, as the co-ordinating institution, we wanted to prepare a project for pupils with ADHD because we know that, across the world, almost 5% of students suffer from this disorder and they encounter difficulties in their academic life, in their community and in their social relationships. Both parents and teachers encounter difficulties while they are supporting pupils with ADHD in their school lives. As the second biggest local education authority in Turkey, we decided to prepare this project for parents and teachers of pupils with ADHD to make everyone’s life easier. We shared our thoughts with different people and institutions from different countries and we realised that it is not only a problem in Turkey but also for other countries. As a result, we developed project partnerships with various educational institutions, universities and non-governmental organisations (NGOs) from different countries. The project development stage was completed after contributions from all partners. Initially we had started with 8 partners but after approval for the project and the agreement of National Agencies of each partner countries, we implemented the project with 6 partners starting from 1st October 2007 to 31st July 2009

# 96. Recobrimento de recessão associada a Festão de McCall através da técnica VISTA

Introdução: A recessão gengival é uma das mais comuns manifestações de doença periodontal e para além de aumentar a sensibilidade dentária, e a incidência de cáries radiculares também provoca discrepâncias na margem gengival o que se traduz num problema estético cada vez mais valorizado. Apesar das muitas técnicas atualmente aceites para o recobrimentos das recessões o enxerto de tecido conjuntivo parece ser a mais abrangente, com mais sucesso e previsibilidade principalmente no que diz respeito a zonas estéticas pois para além de aumentar a espessura de tecido gengival consegue uma cor muito semelhante à da região receptora o que muitas vezes não se consegue com o enxerto gengival livre. Com os avanços efectuados na técnica VISTA consegue‐se um recobrimento sem danos da papila ou do tecido do sulco o que se traduz em resultados mais previsíveis e com pós‐operatórios e resultados estéticos melhorados. Caso clínico: B.S, sexo feminino, 22 anos, fumador, IP inicial 25.8%; diagnosticada com gengivite leve com uma recessão de 2 mm associada a festão de McCall no dente 22 que esteticamente preocupava a paciente. Procedeu‐se à fase higiénica e avaliou‐se a possível etiologia da recessão concluindo‐se que provavelmente se deveria ao facto do trajeto de lateralidade ser feito quase exclusivamente naquele dente, procedeu‐se à eliminação da interferência e avaliou‐se a evolução durante aproximadamente 1 ano. Uma vez que a recessão não desapareceu e que a paciente se apresentava descontente com a estética do seu sorriso planeou‐se a cirurgia periodontal recorrendo a enxerto conjuntivo do tecido do palato segundo a técnica VISTA com uma única incisão localizada no fundo do vestíbulo e sutura suspensa. Repetiu‐se a fase higiénica 24 h antes da cirurgia, a sutura foi removida após 8 dias e repetiu‐se o controlo 8 dias após a remoção da sutura, e 3 meses após a intervenção. Discussão e conclusões: As recessões gengivais são muito comuns na população adulta e idosa contudo também surgem em pacientes jovens e estes apresentam maiores preocupações com as implicações estéticas destas lesões e exigem soluções menos invasivas e com maior previsibilidade. A paciente foi extremamente colaborante, o pós‐operatório foi favorável e os resultados foram muito bons logo ao primeiro controlo, tendo a paciente ficado satisfeita com o resultado final no controlo a 3 meses.info:eu-repo/semantics/publishedVersio

HIPERPLASIA GENGIVAL INDUZIDA POR MEDICAMENTO: RELATO DE CASO

Drug-induced gingival hyperplasia is common in patients undergoing treatment with medications based on phenytoin, nifedipine and cyclosporine-A, due to the fibrotic changes that these drugs cause in the gingival tissues. Therefore, this condition requires more careful hygiene from the patient, and in some situations corrective surgery is necessary to remove hyperplastic gingival tissue, so that the patient has adequate oral health and a more harmonious smile. The objective of the present study is to report a clinical case of corrective surgery by gingivectomy in a patient with drug-treated gingival hyperplasia. Male patient, 14 years old, complained of gingival enlargement. After a detailed anamnesis, he reported the use of phenytoin-based medications, and as a result, it was decided to perform a gingivectomy to remove hyperplastic tissue. After 90 days, excellent healing was achieved and the patient was then released to begin orthodontic treatment. Surgical success was achieved through a correct diagnosis and correct choice of technique.A hiperplasia gengival medicamentosa é comum em pacientes que fazem tratamento com medicamentos à base de fenitoína, nifedipina e ciclosporina-A, devido a alteração fibrótica que essas drogas causam nos tecidos gengivais. Com isso, essa condição exige do paciente uma higienização mais criteriosa, e em algumas situações se faz necessário uma cirurgia corretiva para remoção de tecido gengival hiperplásico, para que o paciente tenha uma saúde bucal adequada e um sorriso mais harmônico. O objetivo do presente estudo é relatar um caso clínico de cirurgia corretiva por gengivectomia em um paciente com hiperplasia gengival medicamentosa. Paciente sexo masculino, 14 anos de idade, queixava-se aumento gengival. Após uma anamnese detalhada, o mesmo relatou sobre o uso de medicamentos à base de fenitoína, e com isso foi optado pela realização de uma gengivectomia para remoção de tecido hiperplásico. Com 90 dias, obteve-se uma excelente cicatrização e o paciente foi liberado então para dar início ao seu tratamento ortodôntico. O sucesso cirúrgico foi obtido por meio de um correto diagnóstico e escolha correta da técnica

Ovarian Torsion in a Primipara Female Dog in Brazil

Background: Ovarian torsion is a condition which the ovary and the ovarian pedicle twist around the ovary suspensory ligament. No report regarding this condition was reported. The aim of the report was to describe a case of unilateral ovarian torsion in a 2-year-old primipara Golden Retriever dog.Case: A 2-year-old female primipara Golden Retriever dog weighting was referred to the Veterinary Hospital, for purulent vaginal discharge evaluation. The dog was presenting anorexia, weight loss, and intermittent diarrhea and vomiting. A cesarean section was performed nine months before her presentation and the oestrus cycle was recorded six months after the cesarean section. Discomfort was observed during the palpation of the abdomen and purulent vaginal was observed. Vaginal swab was performed and followed by cytological examination. The ultrasonographic examination was consistent with pyometra. The ovariohysterectomy was performed and were observed torsion of the left ovary. The histopathological examination of the left ovary and uterus were consistent with ovary necrosis and pyometra. Ten days after surgery the vaginal discharge had resolved and the sutures were removed. Six months postoperatively the dog revealed no further abnormalities. Grossly, the left ovary had firm consistency, dark red surface and 8 cm diameter. The histopathology examination findings were consistent with a diagnosis of diffuse necrosis of left ovary due to ovarian torsion, and uterinesuppurative inflammatory process - pyometra.Discussion: Ovarian torsion is uncommon in small animals, but can be observed high incidence in pregnant female dogs than in non-pregnant ones. The 360º an asynchronous ovarian torsion described in the present case is also an unusual condition in primipara female dogs due to the smaller stretching of the ovary suspensory ligament. The ovarian torsionis considerate an emergency condition due to acute abdominal pain. The mild abdominal discomfort observed during the physical examination was associated to pyometra or to ovarian torsion. The histopathological findings as hemorrhage, edema and necrosis were associated to compromised arterial circulation and ovarian torsion in late stage. The size of the pregnant uterus and/or the pyometra has contributed to ovary torsion. The dog of the present report had pyometra and previouscesarean sections; and these conditions may have contributed to ovarian torsion. The condition was incidentally found during the ovariohysterectomy, and the ovarian torsion diagnosis was determinate through histopathological examination. Computed tomography and magnetic resonance image could be used to diagnose, furthermore, they were not conducted due to the high cost. The leukocytosis and red blood cells Rouleaux was associated with ovarian necrosis and pyometra.Ovariohysterectomy was the treatment of choice to pyometra and ovarian torsion, and the surgery was performed without ovarian torsion reversion to minimize the reperfusion lesions. Ovarian torsion is a rare event in dogs, and it was clinically diagnosed during the surgery. To the best of the authors’ knowledge, this is the first report of ovarian torsion associate withpyometra in primipara female dogs in Brazil.Keywords: dog, obstetric, ovary, pyometra complex, endometrium

PERSPECTIVAS DIDÁTICAS ACERCA DA TEORIA DA RELATIVIDADE

A Teoria da Relatividade é fundamental para o entendimento do mundo contemporâneo e suas tecnologias, por conta disso, se faz fortemente presente nas produções culturais da sociedade. A proposta didática de tratar da Teoria da Relatividade no Ensino Médio utilizando produtos culturais surgiu da necessidade de aproximar o conteúdo dos discentes. O presente trabalho visa relatar a metodologia de pesquisa utilizada no projeto, os resultados parciais obtidos, além da discussãoacerca deles. Como resultado, temos um acervo de tais materiais e um estudo sobre a presença da Teoria da Relatividade nos Institutos Federais do Sul do Brasil

Encefalite secundária à infecção do SARS-CoV-2

Introduction: All the methodological processes that involve the discussion about the neurological complications of Covid-19, and in particular encephalitis, indicate that although the clinical characteristics most observed in patients infected with SARS-CoV-2 are associated with the respiratory system, systemic and systemic changes can also occur in these patients. Methodology: This is an integrative literature review study carried out in the VHL, PMC and IPTODATE, which are online databases of the virtual health library. The following scientific descriptors were used to capture the sample: “Encephaliti”, “viral Encephalophaty” and Covid-19”. The study sample consisted of 54 articles, of which only 19 articles published between the dates of September 2020 and August 2021 comprised the study sample after applying the eligibility criteria. National and international articles available in full were included. Results: The results presented were of great significance regarding the learning of theoretical and practical concepts in the discipline about the occurrence of neurological impacts of Covid-19. Discussion: The study of the subject allowed to observe the neurological complications of covid infection 19, mainly regarding encephalitis and the mechanisms in which Sars-CoV-2 has to reach the central nervous system, its epidemiology, clinical manifestations and treatment. Conclusion: Therefore, it was shown how Sars-CoV-2 virus infection can result in encephalitis, in addition to presenting important data such as its main signs and symptoms, its prevalence and treatment. However, there is still little literature that addresses these repercussions of Covid-19, mainly related to encephalitis. Therefore, more in-depth approaches and research are needed in order to better understand the mechanisms related to the entry of the virus into the central nervous system and how it generates such complications.  Introdução: Todos os processos metodológicos que envolvem a discussão sobre as complicações neurológicas da Covid-19, e em especial a encefalite, indicam que por mais que as características clínicas mais observadas em pacientes infectados pelo SARS-CoV-2 estejam associadas ao sistema respiratório, também podem ocorrer alterações sistêmicas e neurológicas nesses pacientes. Metodologia: Trata-se de um estudo de revisão integrativa da literatura realizado na BVS, PMC e UPTODATE, sendo elas bases de dados online da biblioteca virtual de saúde. Foram utilizados os seguintes descritores científicos para a captação da amostra: “Encephalitis”, “Viral encephalopathy” e “Covid-19”. A amostragem do estudo foi composta por 54 artigos, cujo qual apenas 19 artigos publicados entre as datas de setembro de 2020 a agosto de 2021 compuseram a amostra do estudo após aplicação dos critérios de elegibilidade. Foram incluídos artigos nacionais e internacionais disponíveis na íntegra. Resultados: Os resultados apresentados foram de grande significância com relação ao aprendizado dos conceitos teóricos e práticos na disciplina sobre a ocorrência dos impactos neurológicos da Covid-19. Discussão: O estudo do tema permitiu que fosse observado as complicações neurológicas da infecção do Covid-19, principalmente a respeito da encefalite e os mecanismos em que o Sars-CoV-2 dispõe para atingir o sistema nervoso central, sua epidemiologia, manifestações clínicas e o tratamento. Conclusão: Diante disso, foi mostrado como a infecção pelo vírus Sars-CoV-2 pode resultar na encefalite, além de apresentar dados importantes como os seus principais sinais e sintomas, sua prevalência e tratamento. Entretanto, ainda há pouca literatura que aborda essas repercussões da Covid-19, principalmente relacionado a encefalite. Por isso, se faz necessário abordagens e pesquisas mais aprofundadas a fim de compreender de forma mais explanada os mecanismos relacionados à entrada do vírus no sistema nervoso central e como gera tais complicações. &nbsp