Proof-of-Concept Study of Multifunctional Hybrid Nanoparticle System Combined with NIR Laser Irradiation for the Treatment of Melanoma

Supplementary Materials - available online at https://www.mdpi.com/article/10.3390/biom11040511/s1The global impact of cancer emphasizes the importance of developing innovative, effective and minimally invasive therapies. In the context of superficial cancers, the development of a multifunctional nanoparticle-based system and its in vitro and in vivo safety and efficacy characterization are, herein, proposed as a proof-of-concept. This multifunctional system consists of gold nanoparticles coated with hyaluronic and oleic acids, and functionalized with epidermal growth factor for greater specificity towards cutaneous melanoma cells. This nanoparticle system is activated by a near-infrared laser. The characterization of this nanoparticle system included several phases, with in vitro assays being firstly performed to assess the safety of gold nanoparticles without laser irradiation. Then, hairless immunocompromised mice were selected for a xenograft model upon inoculation of A375 human melanoma cells. Treatment with near-infrared laser irradiation for five minutes combined with in situ administration of the nanoparticles showed a tumor volume reduction of approximately 80% and, in some cases, led to the formation of several necrotic foci, observed histologically. No significant skin erythema at the irradiation zone was verified, nor other harmful effects on the excised organs. In conclusion, these assays suggest that this system is safe and shows promising results for the treatment of superficial melanoma.The authors would like to thank to Fundação para a Ciência e Tecnologia (FCT) for the essential financial support under the project’s references PTDC/BBB-BMC/0611/2012, UIDB/00645/2020, UIDB/04138/2020 and UIDP/04138/2020 as well as for the PhD fellowships SFRH/BD/148044/2019 and SFRH/BD/147306/2019.info:eu-repo/semantics/publishedVersio

A Study of Sawara Marathon at Chiba Normal School

<p>FEV₁: forced expiratory volume in the first second; BMI: body mass index; WC: waist circumference; FM: fat mass; FFM fat free mass. Adjusted by height, weight and skin color current asset index (quintiles), current achieved schooling, smoking status, wheezing in the last year, physical activity and corticoids use in the last three months, birth weight and maternal smoking during pregnancy. P-values for A–D plots: Wald’s test for heterogeneity; p-values for E—H plots: Wald’s test for linear tendency. First quintile: lowest values; fifth quintile: highest values (anthropometric and body composition variables)</p

クリストファー・マーロウ, ジョージ・チャプマン『ヒーローとリアンダー』注解(VI)

<p>Association between FVC (L) and anthropometric variables and body composition, females, at 18 and 30 years.</p

Pathophysiology of major depression by clinical stages

The comprehension of the pathophysiology of the major depressive disorder (MDD) is essential to the strengthening of precision psychiatry. In order to determine the relationship between the pathophysiology of the MDD and its clinical progression, analyzed by severity of the depressive symptoms and sleep quality, we conducted a study assessing different peripheral molecular biomarkers, including the levels of plasma C-reactive protein (CRP), serum mature brain-derived neurotrophic factor (mBDNF), serum cortisol (SC), and salivary cortisol awakening response (CAR), of patients with MDD (n = 58) and a control group of healthy volunteers (n = 62). Patients with the first episode of MDD (n = 30) had significantly higher levels of CAR and SC than controls (n = 32) and similar levels of mBDNF of controls. Patients with treatment-resistant depression (TRD, n = 28) presented significantly lower levels of SC and CAR, and higher levels of mBDNF and CRP than controls (n = 30). An increased severity of depressive symptoms and worse sleep quality were correlated with levels low of SC and CAR, and with high levels of mBDNF. These results point out a strong relationship between the stages clinical of MDD and changes in a range of relevant biological markers. This can assist in the development of precision psychiatry and future research on the biological tests for depression

Uma capitania dos novos tempos: economia, sociedade e política na São Paulo restaurada (1765-1822)

O artigo reflete sobre a trajetória da Capitania de São Paulo, a partir de 1750, apontando sua transformação, de fronteira e "boca do sertão", para território estratégico da conquista e defesa das partes meridionais e área economicamente integrada aos circuitos mercantis atlânticos.In this article, we reflect upon the history of the Captaincy of São Paulo as from 1750, drawing attention to its transformation from frontier land and "door to the backcountry" into a territory of strategic value for the purposes of conquest and defense of the southern regions, and economically integrated into the Atlantic trade routes

O Serviço de documentação textual e iconografia do Museu Paulista

The essay compares the curatorship's works realized during the decade of 1990 by the actual Department of Textual and Iconographical Documentation of Museu Paulista, responsible for the MP Fund / Permanent File (Fundo MP/Arquivo Permanente), hundreds of collections and textual funds and 50.000 iconography pieces, great part of which are gathered in photographic collections. It shows how the documentation work extrapolates the limits of SVDHICO in order to integrate itself with the group activities of the museum and with other research groups. It also points towards new work methodologies which allow to perform the curatorship in an integrated way with the interdisciplinary research and the culture diffusion.O artigo faz um balanço dos trabalhos de curadoria realizados durante a década de 1990 pelo atual Serviço de Documentação Textual e Iconografia do Museu Paulista, responsável pelo Fundo MP/Arquivo Permanente, centenas de coleções e fundos textuais e 50.000 peças de iconografia, grande parte delas reunidas em coleções fotográficas. Mostra como o trabalho de documentação extrapola os limites do SVDHICO para integrar-se com as atividades de conjunto do Museu e com outros grupos de pesquisa. Aponta também para novas metodologias de trabalho com imagens que permitem realizar a curadoria de forma integrada à pesquisa interdisciplinar e à difusão cultural

Detection of dengue virus serotype 3 by reverse transcription-polymerase chain reaction in Aedes aegypti (Diptera, Culicidae) captured in Manaus, Amazonas

The detection of dengue virus serotypes from Aedes aegypti in Manaus, state of Amazonas was carried out using the reverse transcription-polymerase chain reaction technique. Fourteen pools out 82 (17.1%) were positive for DENV3, providing a minimal infection rate of 2.1% of all analyzed infected female specimens of three different areas of the city