317 research outputs found
Recommended from our members
Game of Tenure: the role of “hidden” citations on researchers’ ranking in Ecology
Field ecologists and macroecologists often compete for the same grants and academic positions, with the former producing primary data that the latter generally use for model parameterization. Primary data are usually cited only in the supplementary materials, thereby not counting formally as citations, creating a system where field ecologists are routinely under-acknowledged and possibly disadvantaged in the race for funding and positions. Here, we explored how the performance of authors producing novel ecological data would change if all the citations to their work would be accounted for by bibliometric indicators. We collected the track record of >2300 authors from Google Scholar and citation data from 600 papers published in 40 ecology journals, including field-based, conservation, general ecology, and macroecology studies. Then we parameterized a simulation that mimics the current publishing system for ecologists and assessed author rankings based on number of citations, H-Index, Impact Factor, and number of publications under a scenario where supplementary citations count. We found weak evidence for field ecologists being lower ranked than macroecologists or general ecologists, with publication rate being the main predictor of author performance. Current ranking dynamics were largely unaffected by supplementary citations as they are 10 times less than the number of main text citations. This is further exacerbated by the common practice of citing datasets assembled by previous research or data papers instead of the original articles. While accounting for supplementary citations does not appear to offer a solution, researcher performance evaluations should include criteria that better capture authors’ contribution of new, publicly available data. This could encourage field ecologists to collect and store new data in a systematic manner, thereby mitigating the data patchiness and bias in macroecology studies, and further accelerating the advancement of ecology and related areas of biogeography
A species-level trait dataset of bats in Europe and beyond
Knowledge of species’ functional traits is essential for understanding biodiversity patterns,
predicting the impacts of global environmental changes, and assessing the efficiency of
conservation measures. Bats are major components of mammalian diversity and occupy a
variety of ecological niches and geographic distributions. However, an extensive compilation
of their functional traits and ecological attributes is still missing. Here we present EuroBaTrait
1.0, the most comprehensive and up-to-date trait dataset covering 47 European bat
species. The dataset includes data on 118 traits including genetic composition, physiology,
morphology, acoustic signature, climatic associations, foraging habitat, roost type, diet,
spatial behaviour, life history, pathogens, phenology, and distribution. We compiled the
bat trait data obtained from three main sources: (i) a systematic literature and dataset
search, (ii) unpublished data from European bat experts, and (iii) observations from largescale
monitoring programs. EuroBaTrait is designed to provide an important data source for
comparative and trait-based analyses at the species or community level. The dataset also
exposes knowledge gaps in species, geographic and trait coverage, highlighting priorities for
future data collection.EU Framework Horizon 2020COST Action
CA18107 ‘Climate change and bats: from science to conservation – ClimBats’ (https://climbats.eu/)The Région Bretagne (SAD grant number 19041)Leverhulme Trust (grant
number: ECF-2020-571)Bulgarian National Science Fund (CP-06-COST/15
from 16.12.2020)Natural Environment Research Council Independent Research Fellowship
(NE/M018660/1
Hay testigos: El Golpe
En Hay testigos: El Golpe se hace un recorrido por todo el proceso evolutivo que ha sufrido El Golpe hasta convertirse en lo que es hoy día, una narración audiovisual multimodal publicada a través del perfil en Instagram @haytestigos. Este proyecto no cuenta solo una historia; detalla un trabajo que comenzó hace varios meses y que consta de un proceso de creación de los personajes, redacción de la historia y adaptación de ésta al formato audiovisual, empleando técnicas narrativas propias de este formato; una fase de grabación de la locución de la narración y grabación y montaje de los vídeos que la acompañan; una etapa de diseño del perfil de Instagram y de creación de su contenido (planificación del estilo, creación de la tipografía y sesión de fotografía y posterior edición); una campaña promocional llevada a cabo a través de esta red social y en un lugar físico: la Facultad de Comunicación de Sevilla; una fase de interacción con los seguidores a través de un juego; y, paralelamente, un proceso de investigación periodística de una de las ideas que se cuenta en la historia: el mundo del Sugar Dating.Universidad de Sevilla. Grado en Periodism
Global patterns and predictors of avian population density
Agencia de Innovación y Desarrollo
de Andalucía, Grant/Award Number:
EMERGIA20_00252; H2020 Marie
Skłodowska- Curie Actions, Grant/
Award Number: 891052; Ministero
dell'Istruzione, dell'Università e della
Ricerca, Grant/Award Number: Rita Levi
Montalcini programAim: How population density varies across animal species in the context of environ-
mental gradients, and associated migratory strategies, remains poorly understood.
The recent influx of avian trait data and population density estimates allows these
patterns to be described and explored in unprecedented detail. This study aims to
identify the main macroecological drivers of population density in birds.
Location: Global.
Time period: 1970–2021.
Major taxa studied: Birds (Aves).
Methods: We collated a dataset of 5072 local population density estimates for 1853
species and modelled population density as a function of trait and environmental
predictors in a Bayesian framework accounting for phylogenetic and spatial autocor-
relation. We explored the influence of body mass, diet, primary lifestyle, mating sys-
tem, nesting behaviour, territoriality, and migratory behaviour on population density,
accounting for a range of environmental variables, including preferred habitat type,
primary productivity, precipitation and temperature. Based on this empirical baseline,
we then predicted the mean population density for 9089 species of birds and esti-
mated global geographic patterns of bird population density.
Results: Population density was lower in species with larger body mass and higher
trophic levels, and also declined in territorial species, migratory species, brood para-
sites and species inhabiting resource-poor habitat types (e.g., deserts). Conversely,
population density increased in cooperative breeders. Environmental drivers were
most influential for migratory birds, with precipitation and temperature both associ-
ated with higher population density. Overall, bird population densities were higher at
lower latitudes.
Main conclusions: Our results support previous findings on the role of body mass,
diet and environmental gradients, but also reveal novel species-specific drivers of
avian densities related to reproduction, migration and resource- holding behaviour.
Substantial fine-scale variation remains unexplained. We provide a global dataset of
population density predictions for use in macroecological analyses and conservation
assessments.Agencia de Innovacion y Desarrollo de Andalucia
EMERGIA20_00252H2020 Marie Sklodowska-Curie Actions
891052Ministry of Education, Universities and Research (MIUR
Comparative Analysis of Muscle Transcriptome between Pig Genotypes Identifies Genes and Regulatory Mechanisms Associated to Growth, Fatness and Metabolism.
Iberian ham production includes both purebred (IB) and Duroc-crossbred (IBxDU) Iberian pigs, which show important differences in meat quality and production traits, such as muscle growth and fatness. This experiment was conducted to investigate gene expression differences, transcriptional regulation and genetic polymorphisms that could be associated with the observed phenotypic differences between IB and IBxDU pigs. Nine IB and 10 IBxDU pigs were slaughtered at birth. Morphometric measures and blood samples were obtained and samples from Biceps femoris muscle were employed for compositional and transcriptome analysis by RNA-Seq technology. Phenotypic differences were evident at this early age, including greater body size and weight in IBxDU and greater Biceps femoris intramuscular fat and plasma cholesterol content in IB newborns. We detected 149 differentially expressed genes between IB and IBxDU neonates (p < 0.01 and Fold-Change > 1. 5). Several were related to adipose and muscle tissues development (DLK1, FGF21 or UBC). The functional interpretation of the transcriptomic differences revealed enrichment of functions and pathways related to lipid metabolism in IB and to cellular and muscle growth in IBxDU pigs. Protein catabolism, cholesterol biosynthesis and immune system were functions enriched in both genotypes. We identified transcription factors potentially affecting the observed gene expression differences. Some of them have known functions on adipogenesis (CEBPA, EGRs), lipid metabolism (PPARGC1B) and myogenesis (FOXOs, MEF2D, MYOD1), which suggest a key role in the meat quality differences existing between IB and IBxDU hams. We also identified several polymorphisms showing differential segregation between IB and IBxDU pigs. Among them, non-synonymous variants were detected in several transcription factors as PPARGC1B and TRIM63 genes, which could be associated to altered gene function. Taken together, these results provide information about candidate genes, metabolic pathways and genetic polymorphisms potentially involved in phenotypic differences between IB and IBxDU pigs associated to meat quality and production traits
Self-sufficient flow-biocatalysis by coimmobilization of pyridoxal 5′-phosphate and ω-transaminases onto porous carriers
We expanded the application of self-sufficient heterogeneous biocatalysts containing coimmobilized w-transaminases and pyridoxal 5´-phosphate (PLP) to efficiently operate packed-bed reactors in continuous flow. Using a w-transaminase from Halomonas elongata co-immobilized with PLP onto porous methacrylate-based carriers coated with polyethyleneimine, we operated a packed-bed reactor continuously for up to 50 column volumes at 1.45 mL x min-1 in the enantioselective deamination of model amines (α-methylbenzyl amine), yielding > 90% conversion in all cycles without exogenous addition of cofactor. In this work, we expanded the concept of self-sufficient heterogeneous biocatalysts to other w-transaminases such as the ones from Chromobacterium violaceum and Pseudomonas fluorescens. We found that enzymes with lower affinities towards PLP present lower operational stabilities in flow, even when coimmobilizing PLP. Furthermore, w-transaminases co-immobilized with PLP were successfully implemented for the continuous synthesis of amines and the sustainable metrics were assessed. These results give some clues to exploit PLP-dependent w-transaminases under industrially relevant continuous operations in a more cost-effective and environmentally friendly process
Selective Immobilization of Fluorescent Proteins for the Fabrication of Photoactive Materials
The immobilization of fluorescent proteins is a key technology enabling to fabricate a new generation of photoactive materials with potential technological applications. Herein we have exploited superfolder green (sGFP) and red (RFP) fluorescent proteins expressed with different polypeptide tags. We fused these fluorescent proteins to His-tags to immobilize them on graphene 3D hydrogels, and Cys-tags to immobilize them on porous microparticles activated with either epoxy or disulfide groups and with Lys-tags to immobilize them on upconverting nanoparticles functionalized with carboxylic groups. Genetically programming sGFP and RFP with Cys-tag and His-tag, respectively, allowed tuning the protein spatial organization either across the porous structure of two microbeads with different functional groups (agarose-based materials activated with metal chelates and epoxy-methacrylate materials) or across the surface of a single microbead functionalized with both metal-chelates and disulfide groups. By using different polypeptide tags, we can control the attachment chemistry but also the localization of the fluorescent proteins across the material surfaces. The resulting photoactive material formed by His-RFP immobilized on graphene hydrogels has been tested as pH indicator to measure pH changes in the alkaline region, although the immobilized fluorescent protein exhibited a narrower dynamic range to measure pH than the soluble fluorescent protein. Likewise, the immobilization of Lys-sGFP on alginate-coated upconverting nanoparticles enabled the infrared excitation of the fluorescent protein to be used as a green light emitter. These novel photoactive biomaterials open new avenues for innovative technological developments towards the fabrication of biosensors and photonic devices
Metal substrate catalysis in the confined space for platinum drug delivery
[EN] Catalysis-based approaches for the activation of anticancer agents hold considerable promise. These principally rely on the use of metal catalysts capable of deprotecting inactive precursors of organic drugs or transforming key biomolecules available in the cellular environment. Nevertheless, the efficiency of most of the schemes described so far is rather low, limiting the benefits of catalytic amplification as strategy for controlling the therapeutic effects of anticancer compounds. In the work presented here, we show that flavin reactivity within a hydrogel matrix provides a viable solution for the efficient catalytic activation and delivery of cisplatin, a worldwide clinically-approved inorganic chemotherapy agent. This is achieved by ionically adsorbing a flavin catalyst and a Pt(iv) prodrug as substrate into porous amino-functionalized agarose beads. The hydrogel chassis supplies high local concentrations of electron donating groups/molecules in the surrounding of the catalyst, ultimately boosting substrate conversion rates (TOF >200 min(-1)) and enabling controlled liberation of the drug by light or chemical stimuli. Overall, this approach can afford platforms for the efficient delivery of platinum drugs as demonstrated herein by using a transdermal diffusion model simulating the human skin.We acknowledge financial support from the Spanish State Research Agency (grants CTQ2016-80844-R, PID2019-109111RBI00, RTI2018-094398-B-I00, BIO2014-61838-EXP) and the Basque Government (Eusko Jaurlaritza, grant PIBA_2021_1_0034). S. V. L. thanks the Mexican Council of Science and Technology (CONACyT) for the postdoctoral fellowship she received (ref. CVU-267390). C. S. C. thanks Gipuzkoa Foru Aldundia (Gipuzkoa Fellows program; grant number 2019-FELL-000018-01/62/2019) for.nancial support. L. S. thanks the Spanish MultiMetDrugs network (RED2018-102471-T) for fruitful discussion. FLG thanks the Spanish Biocatalysis network (RED2018-102403T) and the European Research Council (ERC-Co-2018 818089). This work was performed under the Maria de Maeztu and Severo Ochoa Centres of Excellence Programme run by the Spanish State Research Agency, Grant No. MDM-2017-0720 (CIC biomaGUNE) and CEX2018-000867-S (DIPC)
Immunohistochemical expression of aromatase cyp19a1a and cyp19a1b in the ovary and brain of zebrafish (Danio rerio) exposed to different concentrations of bisphenol A
Bisphenol A (BPA) is used to produce plastic and plastic derived products in multitude of daily utensils, being one of the industrial compounds most widely used. This endocrine disrupting chemical (EDCs) is a well-known environmental pollutant released into the aquatic environment from industrial wastewater, sewage sludge or landfill leachate. Aromatases are considered potential targets of EDCs with characteristics that make them suitable biomarkers of exposure to their effects. The main objective of our study was to evaluate the expression of cyp19a aromatase as a toxicological endpoint after BPA exposure through the identification and assessment of alterations of the main cells responsible for cyp19a1a and cyp19a1b expression in the zebrafish ovary and brain using different concentrations of BPA in water. Immunohistochemistry was used to analyze the expression of these enzymes in female zebrafish exposed and not exposed to different concentrations of BPA (1, 10, 100 and 1000 μg / L) in water (n = 6/group) for 14 days. The results obtained in this study showed that the cyp19a aromatase system, involved in the synthesis of steroid compounds, is specially located in distinct oocyte stages in the ovary (cyp19a1a) and in radial glial cells of the brain (cyp19a1b). An overexpression of these aromatases was observed after BPA exposure in zebrafish, peaking from a concentration of 10 µg/L and showing to be good biomarkers of exposure to identify the early effects of low BPA concentrations. To our knowledge, this study is the first to localize and quantify the expression of cyp19a1a and cyp19a1b in the cells of brain and ovary after fish exposure to different BPA concentrations in water
- …