Detection of viable Mycobacterium ulcerans in clinical samples by a novel combined 16S rRNA reverse transcriptase/IS2404 real-time qPCR assay.

Detection of Viable <em>Mycobacterium ulcerans</em> in Clinical Samples by a Novel Combined 16S rRNA Reverse Transcriptase/IS<em>2404</em> Real-Time qPCR Assa

Engineering Characterisation of Aggregates from Some Selected Areas in Kumasi, Ghana

The increase in engineering projects translates to an increase in demand for construction materials, for example, aggregates which are a major component in concrete works. There are many quarries in the Kumasi area which produce aggregates for use in construction works. However, there is no readily available information on the geological and geotechnical properties of these aggregates for use by engineers during the planning, design and construction of projects. This project therefore sought to characterise the aggregate from selected areas (close to some major quarries) in Kumasi based on their geological and engineering properties for construction purposes. Results of the study indicate that Aggregates from sampling locations KP and CS passed the FI test with those from CS being the only ones to pass for EI, making them the best aggregates in terms of Flakiness and Elongation Indices. The aggregates from all the sampling locations passed for the Specific Gravity and Water Absorption tests with CS aggregates giving the best results indicating high strength and good rate of water absorption. Aggregate from sampling location NM gave the best result for the Aggregate Impact Value test indicating high resistance to sudden impacts and shocks. With the Ten Percent Fines Value and the Aggregate Crushing Value Tests, CS yet again produced the aggregates with the best results. The aggregate gave a very high result even under the wet/soaked condition when all the others were giving very low results. Aggregates from this sampling locations can withstand loading gradual compression better than the rest. Finally, the KP aggregates gave the best results for the Los Angeles Abrasion Value which suggests such materials to be the hardest and toughest to resist crushing, degradation and disintegration. Aggregates from the CS, however gave the second best results. From the study, it was concluded that the most suitable location to obtain good quality aggregates is around CS as its aggregates gave the best results in almost all the tests

RSM (Response Surface Methodology) Modelling of Inter-Electrodes Spacing Effects on Phosphate Removal

RSM modelling has been applied in this study to understand the effects of inter-electrodes on the performance of the electrochemical reactors in the removal of pollutants. RSM has been selected because it has the ability to predict the effects of more than one parameter on the targeted variable. Thus, the RSM has been used in this article to model the effects of inter-electrodes spaces (IES) (4 to 10 mm) and treatment time (TT) (5 – 55 min) on the ability of the electrocoagulation (EC) cells to remove phosphate from water. The results showed the best removal of phosphate was 92.5% at I-ES of 4 mm and TT of 50 min. High agreement was noticed between experimental and predicted removals (R 2 = 0.984)

Mycolactone Diffuses into the Peripheral Blood of Buruli Ulcer Patients - Implications for Diagnosis and Disease Monitoring.

BACKGROUND: Mycobacterium ulcerans, the causative agent of Buruli ulcer (BU), is unique among human pathogens in its capacity to produce a polyketide-derived macrolide called mycolactone, making this molecule an attractive candidate target for diagnosis and disease monitoring. Whether mycolactone diffuses from ulcerated lesions in clinically accessible samples and is modulated by antibiotic therapy remained to be established. METHODOLOGY/PRINCIPAL FINDING: Peripheral blood and ulcer exudates were sampled from patients at various stages of antibiotic therapy in Ghana and Ivory Coast. Total lipids were extracted from serum, white cell pellets and ulcer exudates with organic solvents. The presence of mycolactone in these extracts was then analyzed by a recently published, field-friendly method using thin layer chromatography and fluorescence detection. This approach did not allow us to detect mycolactone accurately, because of a high background due to co-extracted human lipids. We thus used a previously established approach based on high performance liquid chromatography coupled to mass spectrometry. By this means, we could identify structurally intact mycolactone in ulcer exudates and serum of patients, and evaluate the impact of antibiotic treatment on the concentration of mycolactone. CONCLUSIONS/SIGNIFICANCE: Our study provides the proof of concept that assays based on mycolactone detection in serum and ulcer exudates can form the basis of BU diagnostic tests. However, the identification of mycolactone required a technology that is not compatible with field conditions and point-of-care assays for mycolactone detection remain to be worked out. Notably, we found mycolactone in ulcer exudates harvested at the end of antibiotic therapy, suggesting that the toxin is eliminated by BU patients at a slow rate. Our results also indicated that mycolactone titres in the serum may reflect a positive response to antibiotics, a possibility that it will be interesting to examine further through longitudinal studies

Does a PBL-based medical curriculum predispose training in specific career paths? A systematic review of the literature

Background North American medical schools have used problem-based learning (PBL) structured medical education for more than 60 years. However, it has only recently been introduced in other medical schools outside of North America. Since its inception, there has been the debate on whether the PBL learning process predisposes students to select certain career paths. Objectives To review available evidence to determine the predisposition of specific career paths when undertaking a PBL-based medical curriculum. The career path trajectory was determined as measured by official Matching Programs, self-reported questionnaires and surveys, and formally defined career development milestones. Methods A systematic literature review was performed. PubMed, Medline, Cochrane and ERIC databases were analysed in addition to reference lists for appropriate inclusion. Results Eleven studies fitting the inclusion criteria were identified. The majority of studies showed that PBL did not predispose a student to a career in a specific speciality (n = 7 out of 11 studies, 64%). However, three studies reported a significantly increased number of PBL graduates working in primary care compared to those from a non-PBL curriculum. Conclusions PBL has been shown not to predispose medical students to a career in General Practice or any other speciality. Furthermore, a greater number of similar studies are required before a definitive conclusion can be made in the future

“It was touching”: Experiences and views of students in the June 3 flood and fire disaster relief response volunteerism in Accra, Ghana

Evidence from Africa on the motivations and experiences of student volunteers in community disaster relief response programs are rare. This study explores the experiences and views of the students who volunteered at the emergency mental health relief response site after the June 3, 2015, flood and fire disaster in Accra, and the implications for future professional response work in Ghana. Thematic analysis of 15 qualitative in-depth interviews showed that, overall, the student volunteers were both self-oriented and other-oriented. The students viewed work at the emergency response site as a touching experience and a call of duty. The response work provided the students with practical pathways to linking their clinical and community learning experiences. This study recommends that, rather than an ad hoc response team, the Ghana Psychological Association should consider setting up a standing disaster relief response network to provide mental health relief and recovery response in community emergency situations in Ghana