Antioxidant activity in selected tomato genotypes cultivated in conventional and organic culture systems

The present study is a compilation of results obtained at the Vegetable Research and Development Station Bacau regarding the influence of the culture system on the quantitative and qualitative yield of tomatoes. The present study provides comparative information regarding yield achievements of tomato genotypes cultivated in two different culture systems (conventional and organic), in order to highlight the suitability of the cultivation system. The best yield results were obtained at a density of 30,000 plants per hectare in both culture systems. All studied genotypes resulted in quantitatively superior yield in the conventional system as opposed to the organic system. Another purpose of this study was to determine the difference in antioxidant activity of tomato genotypes cultivated in the ecological and the conventional culture systems. The results indicate the suitability of the tomato to organic cultivation, highlighting the potential of the tomato species to be utilized as a significant source of natural antioxidants, and also the influence of cultivation systems on the accumulation of antioxidant compounds.Keywords: Lycopersicon esculentum, polyphenols, flavones, yieldAfrican Journal of Biotechnology Vol. 12(20), pp. 2884-289

Effect of AgNO3 on androgenesis of Brassica oleracea L. anthers cultivated in vitro

The present article is a synthesis study accomplished at Vegetable Research and Development Station Bacau regarding the implication of silver nitrate (AgNO3) in modulating the morphogenetic reaction of white cabbage anthers cultivated in vitro. According to literature, silver nitrate is a strong inhibitor of ethylene action. Embryo formation, shoot generation and rooting of plantlets are key points on which relies the entire concept of in vitro tissue culture. Silver ions, in the form of nitrate play an important role in promoting the somatic embryogenesis and organogenesis, which led its wide spread use in different plant tissue culture in vitro. Therefore, our researches focused on testing different concentrations of AgNO3 (5, 10, 15, 25, 30, 50 and 60 μM) added to a pre-tested medium formula– Murashige and Skoog (MS), 1962 basal medium supplemented with benzylaminopurine (BAP)- 8.9 μM and naphthylacetic acid (NAA) 2.7 μM. The biological material is represented by unopened flower buds containing anthers with microspores at late uninucleate to binucleate stage. In the culture condition investigated in this study, the data obtained promotes the utilization of AgNO3 in a concentration of 50 μM for the successfully induction and sustaining of regenerative processes of white cabbage anthers cultivated in vitro.Keywords: Buds, embryogenesis, ethylene, organogenesis, regeneratio

Studiul în faza de răsad a unor soiuri de tomate cultivate în agricultură ecologică

The study was performed in Vegetable Research-Development Station Bacau - Romania, during 2015 – 2016 in order to evaluate the tomato varieties resistance at soil-borne diseases attack in seedling stage. The following tomatoes cultivars were cultivated in protected area - variants V2 – Ghittia, V3 – Monymaker, V4 – Ruxandra, V6 - Inima de bou, V8 – Bobalna, V10 – LMV, V12 – TFC had a degree attack (GA%) below 1.5 %. Other tomatoes cultivars V1 - Brandywine black, V2 - Brandywine black real, V3 - Brandywine black red, V5 – Delicios, V6 - Delicios de Podis, V8 - Indigo ‘Sun’, V9 – Omar’s Lebanese, V10 - Pantene Romanesco, V11 – Thesaloniki, V14 – Vilma were tolerant at the soil borne diseases

Studiul combaterii biologice a tripsului comun la ardei

The trials of tripsattack and ecological control of pests were performed at Vegetable Research and Development Station Bacau –Romania, during 2016 -2017. The dynamic of trips attack in pepperand effectiveness of Amblyseius swirskii At.-H. (Arachnida, Mesostigmata, Phytoseiidae) releases in control of onion tripsat pepper collection of cultivars in tunnelswasstudied. The trial of A. swirskii in tripscontrol was performed at the followingrelease rates: V1 –500,000 mites/ha; V2 –700,000 mites/ha; V3 –900,000 mites/ha; V4. 1 million mites/ha; V5 -Control.On observed that the reducing trips degree attack by release of A. swirskii at pepper is effective in August -September usingthe release rates between 700,000 ex /ha -1,000,000 ex/ ha

Simultaneous Assessment of mTORC1, JAK/STAT, and NLRP3 Inflammasome Activation Pathways in Patients with Sarcoidosis

The unknown etiology of sarcoidosis, along with the variability in organ involvement and disease course, complicates the effective treatment of this disease. Based on recent studies, the cellular inflammatory pathways involved in granuloma formation are of interest regarding possible new treatment options, such as the mechanistic (formerly mammalian) target of rapamycin complex 1 (mTORC1) pathway, the Janus kinase/signal transducers and activators of transcription (JAK/STAT) pathway, and the nucleotide-binding domain, leucine-rich-containing family, pyrin domain-containing-3 (NLRP3) inflammasome pathway. The aim of this study was to explore the potential coexpression of these three inflammatory pathways in patients with sarcoidosis and see whether possible differences were related to disease outcome. The tissue of 60 patients with sarcoidosis was used to determine the activity of these three signaling pathways using immunohistochemistry. The activation of NLRP3 was present in 85% of all patients, and the activation of mTORC1 and JAK/STAT was present in 49% and 50% of patients, respectively. Furthermore, the presence of NLRP3 activation at diagnosis was associated with a chronic disease course of sarcoidosis. Our finding of different new conceptual inflammatory tissue phenotypes in sarcoidosis could possibly guide future treatment studies using the available inhibitors of either NLRP3, JAK-STAT, and mTORC1 inhibitors in a more personalized medicine approach

Soluble Immune Complexes Shift the TLR-Induced Cytokine Production of Distinct Polarized Human Macrophage Subsets towards IL-10

Contains fulltext : 109563.pdf (publisher's version ) (Open Access)BACKGROUND: Costimulation of murine macrophages with immune complexes (ICs) and TLR ligands leads to alternative activation. Studies on human myeloid cells, however, indicate that ICs induce an increased pro-inflammatory cytokine production. This study aimed to clarify the effect of ICs on the pro- versus anti-inflammatory profile of human polarized macrophages. MATERIALS AND METHODS: Monocytes isolated from peripheral blood of healthy donors were polarized for four days with IFN-gamma, IL-4, IL-10, GM-CSF, M-CSF, or LPS, in the presence or absence of heat aggregated gamma-globulins (HAGGs). Phenotypic polarization markers were measured by flow cytometry. Polarized macrophages were stimulated with HAGGs or immobilized IgG alone or in combination with TLR ligands. TNF, IL-6, IL-10, IL-12, and IL-23 were measured by Luminex and/or RT-qPCR. RESULTS: HAGGs did not modulate the phenotypic polarization and the cytokine production of macrophages. However, HAGGs significantly altered the TLR-induced cytokine production of all polarized macrophage subsets, with the exception of MPhi(IL-4). In particular, HAGGs consistently enhanced the TLR-induced IL-10 production in both classically and alternatively polarized macrophages (M1 and M2). The effect of HAGGs on TNF and IL-6 production was less pronounced and depended on the polarization status, while IL-23p19 and IL-12p35 expression was not affected. In contrast with HAGGs, immobilized IgG induced a strong upregulation of not only IL-10, but also TNF and IL-6. CONCLUSION: HAGGs alone do not alter the phenotype and cytokine production of in vitro polarized human macrophages. In combination with TLR-ligands, however, HAGGs but not immobilized IgG shift the cytokine production of distinct macrophage subsets toward IL-10

Non-conventional forms of HLA-B27 are expressed in spondyloarthritis joints and gut tissue

AbstractObjectivesHuman leukocyte antigen (HLA)-B27 (B27) is the strongest genetic factor associated with development of Ankylosing Spondylitis and other spondyloarthropathies (SpA), yet the role it plays in disease pathogenesis remains unclear. We investigated the expression of potentially pathogenic non-conventional heavy chain forms (NC) of B27 in synovial and intestinal tissues obtained from SpA patients. We also determined the presence of NC-B27 in joints, lymphoid and gastrointestinal tissue from B27 transgenic (TG1) rats with M.tuberculosis-induced SpA.MethodsExpression of NC-B27 in human SpA joints and gut and in (21-3 × 283-2)F1 HLA-B27/Huβ2m rat tissue was determined by immunohistochemistry, flow cytometry and confocal microscopy analysis using HC10 and HD6 antibodies.ResultsBoth HC10- and HD6-reactive HLA molecules were present in synovial tissue from SpA patients. Both NC-B27 and KIR3DL2, a ligand for NC-B27, were expressed in inflamed terminal ileal tissues in patients with early SpA. Infiltrating cells in inflamed joint tissues isolated from B27 TG1 rats expressed high levels of NC-B27. NC-B27 were also expressed in joint-resident cells from ankle and tail joints of B27 TG1 rats prior to clinical arthritis. The expression of NC-B27 on B27 TG1 rat CD11b/c+, CD8α+, cells from spleens and LNs increased with animal age and disease progression.ConclusionsNon-conventional HLA class 1 molecules are expressed on resident and infiltrating cells in both synovial and GI tissues in human SpA. NC-B27 expression in joints and lymphoid tissues from B27 TG1 rats prior to the onset of arthritis is consistent with the hypothesis that they play a pathogenic role in SpA