Layer-by-layer coated silicone-based soft contact lens hydrogel for diclofenac sustained release

Abstract in proceedings of the Fourth International Congress of CiiEM: Health, Well-Being and Ageing in the 21st Century, held at Egas Moniz’ University Campus in Monte de Caparica, Almada, from 3–5 June 2019.This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.info:eu-repo/semantics/publishedVersio

Fractal analysis of growing cities and its relationship with health centre

During recent years, many research group interested in the study of organizational networks from different fields, e.g. studies of behavior of cities growth following the fractal theory. Considering the fractal analysis as a strong tools to analyse the relation between grow of cities and its relation with health center distribution. The propose of this paper is to develop a methodology based on a fractal analysis framework of Chilean public health networks, the population growth of cities and the location of health centers. To produce empirical approach for the analysis of the locations of health centers and its effects. On the other hand the health has been related to other sciences, a clear example is the physics and the mathematics, where we found many studies related with the geometry to understood the nature of the multiple natural shapes, its developed was called fractal nature or mathematic fractal model, this approach consider the different scales. For this reason, we present in this paper some preliminary ideas and some initial results of the analysis of the complex and fractal behavior of urban expansion, and its relation to the location of public health centers through simple counters algorithms and spectral methods using ImaCal software. The study health patterns will become a useful tool for understanding human flow and behavior of health center, where using a traditional measurement as equipment has serious problems and limitations.Peer ReviewedPostprint (published version

NO2-controlled cargo delivery from gated silica mesoporous nanoparticles

[EN] Cargo delivery from mesoporous silica nanoparticles loaded with sulforhodamine B and capped with a difluoroboron-dipyrromethene (BODIPY) derivative was triggered by a NO2-induced oxidative process.The authors thank the financial support from the Spanish Government (project MAT2015-64139-C4-R) and the Generalitat Valenciana (project GVA/2014/13).Juarez, LA.; Costero, AM.; Parra Alvarez, M.; Gaviña, P.; Gil Grau, S.; Martínez-Máñez, R.; Sancenón Galarza, F. (2017). NO2-controlled cargo delivery from gated silica mesoporous nanoparticles. Chemical Communications. 53(3):585-588. https://doi.org/10.1039/c6cc08885fS585588533ARIGA, K., VINU, A., HILL, J., & MORI, T. (2007). Coordination chemistry and supramolecular chemistry in mesoporous nanospace. Coordination Chemistry Reviews, 251(21-24), 2562-2591. doi:10.1016/j.ccr.2007.02.024Katz, E., & Willner, I. (2004). Integrated Nanoparticle-Biomolecule Hybrid Systems: Synthesis, Properties, and Applications. 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ChemistryOpen, 4(4), 418-437. doi:10.1002/open.201500053Casasús, R., Aznar, E., Marcos, M. D., Martínez-Máñez, R., Sancenón, F., Soto, J., & Amorós, P. (2006). New Methods for Anion Recognition and Signaling Using Nanoscopic Gatelike Scaffoldings. Angewandte Chemie International Edition, 45(40), 6661-6664. doi:10.1002/anie.200602045Coll, C., Casasús, R., Aznar, E., Marcos, M. D., Martínez-Máñez, R., Sancenón, F., … Amorós, P. (2007). Nanoscopic hybrid systems with a polarity-controlled gate-like scaffolding for the colorimetric signalling of long-chain carboxylates. Chem. Commun., (19), 1957-1959. doi:10.1039/b617703dÖzalp, V. C., & Schäfer, T. (2011). Aptamer-Based Switchable Nanovalves for Stimuli-Responsive Drug Delivery. Chemistry - A European Journal, 17(36), 9893-9896. doi:10.1002/chem.201101403Climent, E., Marcos, M. D., Martínez-Máñez, R., Sancenón, F., Soto, J., Rurack, K., & Amorós, P. (2009). The Determination of Methylmercury in Real Samples Using Organically Capped Mesoporous Inorganic Materials Capable of Signal Amplification. Angewandte Chemie International Edition, 48(45), 8519-8522. doi:10.1002/anie.200904243Wen, Y., Xu, L., Li, C., Du, H., Chen, L., Su, B., … Song, Y. (2012). DNA-based intelligent logic controlled release systems. Chemical Communications, 48(67), 8410. doi:10.1039/c2cc34501cZhang, Z., Balogh, D., Wang, F., & Willner, I. (2013). Smart Mesoporous SiO2 Nanoparticles for the DNAzyme-Induced Multiplexed Release of Substrates. Journal of the American Chemical Society, 135(5), 1934-1940. doi:10.1021/ja311385yZhou, Y., Tan, L.-L., Li, Q.-L., Qiu, X.-L., Qi, A.-D., Tao, Y., & Yang, Y.-W. (2014). Acetylcholine-Triggered Cargo Release from Supramolecular Nanovalves Based on Different Macrocyclic Receptors. Chemistry - A European Journal, 20(11), 2998-3004. doi:10.1002/chem.201304864Chen, M., Huang, C., He, C., Zhu, W., Xu, Y., & Lu, Y. (2012). A glucose-responsive controlled release system using glucose oxidase-gated mesoporous silica nanocontainers. Chemical Communications, 48(76), 9522. doi:10.1039/c2cc34290aCliment, E., Bernardos, A., Martínez-Máñez, R., Maquieira, A., Marcos, M. D., Pastor-Navarro, N., … Amorós, P. (2009). Controlled Delivery Systems Using Antibody-Capped Mesoporous Nanocontainers. Journal of the American Chemical Society, 131(39), 14075-14080. doi:10.1021/ja904456dYang, S., Li, N., Liu, Z., Sha, W., Chen, D., Xu, Q., & Lu, J. (2014). Amphiphilic copolymer coated upconversion nanoparticles for near-infrared light-triggered dual anticancer treatment. Nanoscale, 6(24), 14903-14910. doi:10.1039/c4nr05305bSchloßbauer, A., Sauer, A. M., Cauda, V., Schmidt, A., Engelke, H., Rothbauer, U., … Bein, T. (2012). Cascaded Photoinduced Drug Delivery to Cells from Multifunctional Core-Shell Mesoporous Silica. Advanced Healthcare Materials, 1(3), 316-320. doi:10.1002/adhm.201100033Mackowiak, S. A., Schmidt, A., Weiss, V., Argyo, C., von Schirnding, C., Bein, T., & Bräuchle, C. (2013). Targeted Drug Delivery in Cancer Cells with Red-Light Photoactivated Mesoporous Silica Nanoparticles. Nano Letters, 13(6), 2576-2583. doi:10.1021/nl400681fSilveira, G. Q., Vargas, M. D., & Ronconi, C. M. (2011). Nanoreservoir operated by ferrocenyl linker oxidation with molecular oxygen. Journal of Materials Chemistry, 21(16), 6034. doi:10.1039/c0jm03738aColl, C., Bernardos, A., Martínez-Máñez, R., & Sancenón, F. (2012). Gated Silica Mesoporous Supports for Controlled Release and Signaling Applications. Accounts of Chemical Research, 46(2), 339-349. doi:10.1021/ar3001469El Sayed, S., Milani, M., Milanese, C., Licchelli, M., Martínez-Máñez, R., & Sancenón, F. (2016). Anions as Triggers in Controlled Release Protocols from Mesoporous Silica Nanoparticles Functionalized with Macrocyclic Copper(II) Complexes. 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(2016). 3-Formyl-BODIPY Phenylhydrazone as a Chromo-Fluorogenic Probe for Selective Detection of NO2(g). Chemistry - A European Journal, 22(25), 8448-8451. doi:10.1002/chem.20160092

A New Highly Selective Chromogenic and Fluorogenic Chemosensor for Copper (II)

[EN] A new fluorogenic and chromogenic probe (L) for the selective, sensitive and naked-eye detection of Cu2+ is reported. Complexation constant, complex stoichiometry and quantum chemical (DFT) calculation for Cu2+ complex has been determined. Also, detection limits and the selectivity in front of other divalent and trivalent cations have been evaluated.This research was supported by the Agencia Nacional de Ciencia y Tecnologia (ANCyT) of Argentina-PICT 2014 No. 1587 and by the Universidad Nacional del Litoral, Santa Fe, Argentina.Quindt, MI.; Gutiérrez, LG.; Kneeteman, MN.; Mancini, P.; Parra Alvarez, M.; Gil Grau, S.; Costero, AM. (2018). A New Highly Selective Chromogenic and Fluorogenic Chemosensor for Copper (II). Letters in Organic Chemistry. 15(8):659-664. https://doi.org/10.2174/1570178615666180102155804S65966415

Electron Microscopy Structural Insights into CPAP Oligomeric Behavior:A Plausible Assembly Process of a Supramolecular Scaffold of the Centrosome

Centrosomal P4.1-associated protein (CPAP) is a cell cycle regulated protein fundamental for centrosome assembly and centriole elongation. In humans, the region between residues 897–1338 of CPAP mediates interactions with other proteins and includes a homodimerization domain. CPAP mutations cause primary autosomal recessive microcephaly and Seckel syndrome. Despite of the biological/clinical relevance of CPAP, its mechanistic behavior remains unclear and its C-terminus (the G-box/TCP domain) is the only part whose structure has been solved. This situation is perhaps due in part to the challenges that represent obtaining the protein in a soluble, homogeneous state for structural studies. Our work constitutes a systematic structural analysis on multiple oligomers of HsCPAP897−1338, using single-particle electron microscopy (EM) of negatively stained (NS) samples. Based on image classification into clearly different regular 3D maps (putatively corresponding to dimers and tetramers) and direct observation of individual images representing other complexes of HsCPAP897−1338 (i.e., putative flexible monomers and higher-order multimers), we report a dynamic oligomeric behavior of this protein, where different homo-oligomers coexist in variable proportions. We propose that dimerization of the putative homodimer forms a putative tetramer which could be the structural unit for the scaffold that either tethers the pericentriolar material to centrioles or promotes procentriole elongation. A coarse fitting of atomic models into the NS 3D maps at resolutions around 20 Å is performed only to complement our experimental data, allowing us to hypothesize on the oligomeric composition of the different complexes. In this way, the current EM work represents an initial step toward the structural characterization of different oligomers of CPAP, suggesting further insights to understand how this protein works, contributing to the elucidation of control mechanisms for centriole biogenesis.This work was supported by the Comunidad de Madrid through grant CAM (S2010/BMD- 2305) and the Spanish Ministry of Economy and Competitiveness through Grants AIC-A-2011-0638 and BIO2013-44647-R.Peer reviewedPeer Reviewe

Effect of red beet and betaine modulating oxidation and bioactivity of rainbow trout

[EN] The present study compares a control diet to four experimental diets, in which two red beet (14% and 28%) and two betaine levels (0.9% and 1.63%) were incorporated in rainbow trout diets according to a factorial design. The effects of the inclusion of different red beet and betaine concentrations on fatty acid (FA) profile, lipid peroxidation, and antioxidant activity on rainbow trout fillets were investigated. Although no significant differences were observed with the control group, results indicated that red beet and betaine improved fish fillet FA profile, producing an increase in polyunsaturated FAs, mainly docosahexaenoic acid. Higher red beet and betaine concentrations increased flavonoid and phenolic content in the diets; however, no effects were observed for the antioxidant properties of rainbow trout fillets.This work was carried out with funding from INIA and ITACyL, and cofounded by FEDER funds. Julia Pinedo has been granted with the FPI-INIA grant number 21 (call 2012, BOE-2012-13337).Pinedo-Gil, J.; Tomas-Vidal, A.; Rico-Barges, D.; Tiwari B.K.; Alvarez-García C; Jover Cerda, M.; Sanz-Calvo MA... (2019). Effect of red beet and betaine modulating oxidation and bioactivity of rainbow trout. Journal of Aquatic Food Product Technology. 38-48. https://doi.org/10.1080/10498850.2018.1560381S384

Chromogenic Chemodosimeter Based on Capped Silica Particles to Detect Spermine and Spermidine

[EN] A new hybrid organic-inorganic material for sensing spermine (Spm) and spermidine (Spd) has been prepared and characterized. The material is based on MCM-41 particles functionalized with an N-hydroxysuccinimide derivative and loaded with Rhodamine 6G. The cargo is kept inside the porous material due to the formation of a double layer of organic matter. The inner layer is covalently bound to the silica particles, while the external layer is formed through hydrogen and hydrophobic interactions. The limits of detection determined by fluorimetric titration are 27 mu M and 45 mu M for Spm and Spd, respectively. The sensor remains silent in the presence of other biologically important amines and is able to detect Spm and Spd in both aqueous solution and cells.This research was funded by Spanish Government (RTI2018-100910-B-C42 and RTI2018100910-B-C44 (MCUI/AEI/FEDER, UE) and grant GRISOLIAP/2019/023.Barros, M.; López-Carrasco, A.; Amorós, P.; Gil Grau, S.; Gaviña, P.; Parra Alvarez, M.; El Haskouri, J.... (2021). Chromogenic Chemodosimeter Based on Capped Silica Particles to Detect Spermine and Spermidine. Nanomaterials. 11(3):1-12. https://doi.org/10.3390/nano1103081811211

Sex differences in the aging pattern of renin–angiotensin system serum peptidases

Background: Serum peptidases, such as angiotensin-converting enzyme (ACE), angiotensin-converting enzyme-2 (ACE2), neutral endopeptidase (NEP), aminopeptidase N (APN), and aminopeptidase A (APA), are important elements of the renin-angiotensin system (RAS). Dysregulation of these enzymes has been associated with hypertension and cardiovascular risk. In the present study, serum activities of RAS peptidases were analyzed to evaluate the existence of sexual differences, with a possible different pattern in pre- and post-andropausal/post-menopausal participants. Methods: One hundred and eighteen healthy men and women between 41 and 70 years of age (58 women and 60 men) were recruited to participate in the study. Serum RAS-regulating enzymes were measured by spectrofluorimetry. Enzymatic activity was recorded as units of enzyme per milliliter of serum (U/mL). Results: Significantly lower serum APA activity was observed in men with respect to women; no sex differences were detected for ACE, ACE2, NEP, or APN. Significantly lower APA and ACE serum activity were observed in older men compared to older women. In contrast, younger (<55 years) men had significantly higher values of NEP serum activity than younger women. Significantly lower ACE serum activity was detected in older men compared to younger men. In women, significantly higher ACE2 serum activity was observed in older women compared to younger women. Conclusions: These results suggest a differential effect of aging on the activity of RAS enzymes in men and women, especially with respect to the breakpoint of andropausia/menopausia, on the critical serum enzymatic activities of the RAS, which could correlate with sexual differences in cardiovascular risk.This work was supported by a grant from the Basque Government (IT8-11/13), the University of the Basque Country UPV/EHU (UFI 11/44), and the Gangoiti-Barrera Foundation

Obesity Parameters, Physical Activity, And Physical Fitness are Correlated With Serum Dipeptidyl Peptidase IV Activity In A Healthy Population

Objective: To determine whether obesity, physical fitness, and physical activity parameters are associated with the enzymatic activity of serum dipeptidyl peptidase IV (sDPPIV) in a sample of healthy women and men. Design and methods: We have correlated parameters of obesity, physical fitness, and physical activity with sDPPIV activity in 374 healthy subjects (age: 60.7 +/- 6.9 years, body mass index: 26.1 +/- 4.1 kg/m(2)). Enzymatic activity was analyzed using spectrofluorimetry, body composition was assessed by impedanciometry, physical fitness data were obtained using the Senior Fitness Test, and physical activity data were collected by accelerometer. Pearson' s partial correlation analysis was applied to determine the relationship between DPPIV activity and the rest of parameters and significantly correlated variables were introduced into linear regression models to predict DPPIV. Results: Serum DPPIV activity was negatively associated with obesity parameters such as body mass (r = -0.112), body mass index (BMI) (r = -0.147), waist circumference (r = -0.164), waist-to-hip ratio (-0.104), and percentage of fat mass (r = -0.185). Serum DPPIV activity was positively associated with cardiovascular fitness (r = 0.138), total amount of physical activity (r = 0.153), and time spent doing light exercise (r = 0.184). Regression models revealed sex differences in enzyme activity with overall activity higher in women than in men (beta = 0.437, p < 0.001). Further, percent fat mass was an independent negative predictor of DPPIV activity (beta = -0.184, p = 0.001). Serum DPPIV activity was positively predicted based on the amount of time spent doing light physical activity (beta = 0.167, p = 0.001). Conclusion: Our results demonstrate that sDPPIV activity is positively associated with healthier parameters regarding fatness, fitness and physical activity.This work was supported by the Basque Government (GIC12/173: IT811-13) and the University of the Basque Country (UPV/EHU: PPG17/40)

История изучения имен прилагательных в крымскотатарском языке

Целью данной статьи является рассмотрение степени изученности имени прилагательного в крымскотатарском языке как самостоятельной части речи в лингвистических трудах XIX – XX вв., а также анализ основных грамматических категорий имени прилагательного