Nanocrystallization of the Cd3Al2Ge3O12 Garnet in Glasses of the CdO-TeO2-GeO2 System

In this study, a series of glasses of the system xCdO-10TeO2-(90−x)GeO2 were fabricated, varying the modifier oxide content from 10 to 80 wt%. According to XRD analysis, partial crystallization occurred for the glass 60CdO.10TeO2.30GeO2 presenting the formation of GeO2 and CdTeO3; the 70CdO.10TeO2.20GeO2 glass shows sharp diffraction peaks corresponding to the Cd3Al2Ge3O12 garnet crystalline phase. Transmission electron microscopy showed that the garnet crystals have sizes below 20 nm. At the highest concentration of CdO (80 wt%), a transparent orange glass can be obtained, and this sample can be identified as an inverted glass where CdO participates as a network former. The optical band gap of the glasses decreases as CdO content increases from 3.91 to 3.0 eV. In general, all glasses show a typical broad emission when excited with UV light (325 nm); chromatic coordinates were calculated and pointed out the presence of emissions in the white, green, and yellow regions. In summary, the obtained glasses are a promising material for IR technologies, nonlinear optics, and design of solid state lighting devices

A proteomics approach to decipher the molecular nature of planarian stem cells

Background In recent years, planaria have emerged as an important model system for research into stem cells and regeneration. Attention is focused on their unique stem cells, the neoblasts, which can differentiate into any cell type present in the adult organism. Sequencing of the Schmidtea mediterranea genome and some expressed sequence tag projects have generated extensive data on the genetic profile of these cells. However, little information is available on their protein dynamics. Results We developed a proteomic strategy to identify neoblast-specific proteins. Here we describe the method and discuss the results in comparison to the genomic high-throughput analyses carried out in planaria and to proteomic studies using other stem cell systems. We also show functional data for some of the candidate genes selected in our proteomic approach. Conclusions We have developed an accurate and reliable mass-spectra-based proteomics approach to complement previous genomic studies and to further achieve a more accurate understanding and description of the molecular and cellular processes related to the neoblasts

Transcriptome Analysis Reveals Strain-Specific and Conserved Stemness Genes in Schmidtea mediterranea

The planarian Schmidtea mediterranea is a powerful model organism for studying stem cell biology due to its extraordinary regenerative ability mediated by neoblasts, a population of adult somatic stem cells. Elucidation of the S. mediterranea transcriptome and the dynamics of transcript expression will increase our understanding of the gene regulatory programs that regulate stem cell function and differentiation. Here, we have used RNA-Seq to characterize the S. mediterranea transcriptome in sexual and asexual animals and in purified neoblast and differentiated cell populations. Our analysis identified many uncharacterized genes, transcripts, and alternatively spliced isoforms that are differentially expressed in a strain or cell type-specific manner. Transcriptome profiling of purified neoblasts and differentiated cells identified neoblast-enriched transcripts, many of which likely play important roles in regeneration and stem cell function. Strikingly, many of the neoblast-enriched genes are orthologs of genes whose expression is enriched in human embryonic stem cells, suggesting that a core set of genes that regulate stem cell function are conserved across metazoan species

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Hedgehog signaling is critical for vertebrate central nervous system (CNS) development, but its role in CNS biology in other organisms is poorly characterized. In the planarian Schmidtea mediterranea, hedgehog (hh) is expressed in medial cephalic ganglia neurons, suggesting a possible role in CNS maintenance or regeneration. We performed RNA sequencing of planarian brain tissue following RNAi of hh and patched (ptc), which encodes the Hh receptor. Two misregulated genes, intermediate filament-1 (if-1) and calamari (cali), were expressed in a previously unidentified non-neural CNS cell type. These cells expressed orthologs of astrocyte-associated genes involved in neurotransmitter uptake and metabolism, and extended processes enveloping regions of high synapse concentration. We propose that these cells are planarian glia. Planarian glia were distributed broadly, but only expressed if-1 and cali in the neuropil near hh[superscript +] neurons. Planarian glia and their regulation by Hedgehog signaling present a novel tractable system for dissection of glia biology.National Institutes of Health (U.S.) (NIH (R01GM080639))Howard Hughes Medical Institute (Investigator

Genome-Wide Analyses Reveal a Role for Peptide Hormones in Planarian Germline Development

Genomic/peptidomic analyses of the planarian Schmidtea mediterranea identifies >200 neuropeptides and uncovers a conserved neuropeptide required for proper maturation and maintenance of the reproductive system

Sistema de actividades para la implementación de la perspectiva de género en el programa de Historia de Cuba. (Original)

The present work has as objective the elaboration of a system of activities that is sustained in the General Theory of the Systems and forms part of the institutional research project that was developed in the University of Granma between the years 2016 and 2017 called "The familiar education and gender, its expression in local development ". The proposed system is structured in two subsystems, the first referred to the period of 1867-1878 and the second subsystem includes activities related to other women who are not in the locality, through it must achieve an assimilation of knowledge of the different events, historical facts and figures of the colonial period of the History of Cuba with emphasis on the role of women, so it offers prospective graduates alternatives for the teaching-learning process of the subject.El presente trabajo tiene como objetivo la elaboración de un sistema de actividades que se sustenta en la Teoría General de los Sistemas y forma parte del proyecto de investigación institucional que se desarrolló en la Universidad de Granma entre los años 2016 y 2017 denominado “La educación familiar y el género, su expresión en el desarrollo local”. El sistema propuesto se estructura en dos subsistemas,el primero referido al período de 1867-1878  y en el segundo subsistema se incluyen actividades relacionadas con otras mujeres destacadas que no son de la localidad, a través del mismo se debe lograr una asimilación de los conocimientos de los diferentes acontecimientos, hechos históricos y figuras de la etapa colonial de la Historia de Cuba con énfasis en el papel de la mujer, por lo que ofrece a los futuros egresados alternativas para el proceso de enseñanza-aprendizaje de la asignatura

Contribuciones laborales de una empresa con giro electrónico

Seminario: Diagnóstico financiero de la empres