Phenology of aquatic insects in a protected wetland (Natura 2000 network) in northwestern Spain

The aim of this study was to gather new data about the life cycle phenologies of several species of aquatic insects in the "Gándaras de Budiño" (Galicia, NW Spain) protected wetland, included in the Natura 2000 network. During an annual cycle (2004-2005), three shallow lakes and four streams were sampled monthly using a semi-quantitative sampling method. The body lengths of the larvae and nymphs of thirteen species were measured, and their life cycles were analysed. All species had univoltine or semivoltine cycles. Additionally, a possible correlation between larval and nymphal lengths and water temper­ature was examined. We found a significant correlation for two species: the water beetle (Note rus laevis) and the dragonfly (Boyeria Irene). It is important to highlight the presence in the studied area of a species included in the Habitats Directive: the damselfly (Coenagrion mercuriale).El objetivo principal de este estudio fue aportar nuevos datos al conocimiento sobre los ciclos de vida de varias especies de insectos acuáticos en un humedal protegido, las Gándaras de Budiño (Galicia, NO España), incluido en la Red Natura 2000. Se muestrearon tres lagunas y cuatro arroyos mensualmente durante un año (2004-2005) mediante un método semicuanti­tativo. Se midieron las larvas y ninfas de trece especies, y se analizaron sus ciclos de vida. Todas las especies presentaron ciclos univoltinos o semivoltinos. También se examinó la posible correlación entre la longitud de las larvas y ninfas, y la temperatura del agua. Se encontró una correlación significativa para dos especies, el coleóptero acuático Noterus laevis, y la libélula (Boyeria irene). Es importante destacar la presencia de una especie incluida en la Directiva Hábitats, el caballito del diablo (Coenagrion mercuriale)

Comparación de la comunidad de coleópteros acuáticos (Adephaga y Polyphaga) en dos cuencas hidrográficas con distinto grado de acción antropogénica (Pontevedra, NO de España)

En este trabajo se estudia la fauna de coleópteros acuáticos (Adephaga y Polyphaga) de las cuencas hidrográficas de los ríos Lagares y Miñor (Pontevedra, NO de España). Se muestrearon 20 puntos en cuatro campañas estacionales (mayo 2001-enero 2002). Se estudiaron un total de 1597 individuos, identificándose 35 especies de las familias Haliplidae, Gyrinidae, Dytiscidae, Helophoridae, Hydrochidae, Hydrophilidae, Hydraenidae, Elmidae, Dryopidae y Scirtidae. Se citan por primera vez para la provincia de Pontevedra la familia Hydrochidae (Hydrochus angustatus) los géneros Esolus (Esolus parallelepipedus) y Megasternum (Megasternum concinnum) y las especies Helophorus flavipes, Elmis aenea y Elmis maugetii maugetii. La variación espacial y temporal de la riqueza, abundancia y diversidad han sido analizadas, así como la afinidad entre los puntos de muestreo, basada en el análisis de la composición faunística. Los resultados indican que las comunidades típicas de aguas no contaminadas quedan claramente separadas de las comunidades de aguas contaminadas, indicando este fenómeno la influencia negativa de la contaminación sobre la fauna.In this work we studied the fauna of aquatic Coleoptera (Adephaga and Polyphaga) of the hydrographic river basins of the Lagares and Miñor Rivers (Pontevedra, NW of Spain). Twenty sites were sampled in four seasonal campaigns between May of 2001 and January of 2002. A total of 1,597 specimens belonging to 35 species of the families Haliplidae, Gyrinidae, Dytiscidae, Helophoridae, Hydrochidae, Hydrophilidae, Hydraenidae, Elmidae, Dryopidae and Scirtidae were collected. The family Hydrochidae (Hydrochus angustatus), the genus Esolus (Esolus parallelepipedus), and Megasternum (Megasternum concinnum), and the species Helophorus flavipes, Elmis aenea, and Elmis maugetii maugetii are all new records for the province. Spatial and seasonal variations in richness, abundance and diversity were analysed, in addition the similarity between sites was also analysed based on the total fauna composition of each. The results indicate that the typical communities of non-polluted waters are separated from those of polluted waters, indicating the negative influence of the contamination on the fauna

Fear of death and its relationship to resilience in nursing students: A longitudinal study

.Aim Taking a corpus of nursing students enrolled in the 2017−2021 nursing degree, we aim to analyse how students' levels of resilience and fear of death evolve in the first three years of the degree and whether there are differences between students based on age and gender. In addition, we aim to describe the relationship between resilience and fear of deathS

Modelling the Biphasic Growth and Product Formation by Enterococcus faecium CECT 410 in Realkalized Fed-Batch Fermentations in Whey

The influence of initial pH on growth and nutrient (total sugars, nitrogen, and phosphorous) consumption by Enterococcus faecium CECT 410 was studied during batch cultures in whey. With these data, two realkalized fed-batch fermentations were developed using different feeding substrates. The shift from homolactic to mixed acid fermentation, the biphasic kinetics observed for cell growth and nitrogen consumption and the increase in the concentrations of biomass and products (lactic acid, acetic acid, ethanol, and butane-2,3-diol) were the most noteworthy observations of these cultures. Modelling the fed-batch growth of Ent. faecium with the Logistic and bi-Logistic models was not satisfactory. However, biomass production was best mathematically described with the use of a double Monod model, which was expressed in terms of biomass, product accumulation, and nitrogen utilization. Product formation was successfully modelled with a modified form of the Luedeking and Piret model developed in this study

Serum nuclear magnetic resonance metabolomics analysis of human metastatic colorectal cancer: Biomarkers and pathway analysis

Junta de Andalucía, Grant/Award Numbers: 102C2000004, UAL2020-AGR-B1781, P20_01041; Gobierno de España, Grant/Award Numbers: PDC2021– 121248-I00, PLEC2021–007774; Instituto de Salud Carlos III (ISCIII), Grant/Award Number: PI19/01478; CTS-107 and FQM-376 groupsWe describe the use of nuclear magnetic resonance metabolomics to analyze blood serum samples from healthy individuals (n = 26) and those with metastatic colorectal cancer (CRC; n = 57). The assessment, employing both linear and nonlinear multivari- ate data analysis techniques, revealed specific metabolite changes associated with metastatic CRC, including increased levels of lactate, glutamate, and pyruvate, and decreased levels of certain amino acids and total fatty acids. Biomarker ratios such as glutamate-to-glutamine and pyruvate-to-alanine were also found to be related to CRC. The study also found that glutamate was linked to progression-free survival and that both glutamate and 3-hydroxybutyrate were risk factors for metastatic CRC. Additionally, gas chromatography coupled to flame-ionization detection was utilized to analyze the fatty acid profile and pathway analysis was performed on the profiled metabolites to understand the metabolic processes involved in CRC. A correlation was also found between the presence of certain metabolites in the blood of CRC patients and certain clinical features.Junta de Andalucia 102C2000004, UAL2020-AGR-B1781, P20_01041Gobierno de España MCIN/AEI/10.13039/501100011033/Unión Europea “Next GenerationEU”/PRTR (PDC2021–121248-I00 and PLEC2021–007774)Instituto de Salud Carlos III (ISCIII) (PI19/01478) (FEDER)CTS-107FQM-37

Monitoring Coxiella burnetii Infection in Naturally Infected Dairy Sheep Flocks Throughout Four Lambing Seasons and Investigation of Viable Bacteria.

Progression of Coxiella burnetii infection in four naturally infected sheep flocks, and in their farm environment, was monitored throughout four lambing seasons. Flocks with an active infection were selected based on the presence of C. burnetii DNA in bulk-tank milk (BTM) and a high seroprevalence in yearlings during the previous milking period (Spring 2015). During four consecutive lambing seasons (2015/16-2018/19), samples were collected within 1 week after each lambing period from animals (vaginal swabs, milk and feces from ewes, and yearlings) and the environment (dust indoor sheep premises). BTM samples and aerosols (outdoors and indoors) were monthly collected between lambing and the end of milking. Real-time PCR analyses showed different trends in C. burnetii shedding in the flocks, with a general progressive decrease in bacterial shedding throughout the years, interrupted in three flocks by peaks of reinfection associated with specific management practices. A significant relationship was found between C. burnetii fecal shedding and the bacterial burden detected in dust, whereas shedding by vaginal route affected the detection of C. burnetii in indoor aerosols. Three genotypes were identified: SNP8 (three flocks, 52.9% of the samples), SNP1 (two flocks, 44.8% samples), and SNP5 (one flock, two environmental samples). Coxiella burnetii viability in dust measured by culture in Vero cells was demonstrated in two of the flocks, even during the fourth lambing season. The results showed that infection can remain active for over 5 years if effective control and biosafety measures are not correctly implemented.This work was funded by INIA—Spanish National Institute for Agricultural and Food Research and Technology (RTA2017-00055-C02-00), the European Regional Development Funds (ERDF), and the Basque Government. RÁ-A is beneficiary of a Ph.D. contract funded by INIA (FPI-2015-014). The funders had no role in the study design, data collection and interpretation, or the decision to submit the work for publication.S