FOTOCATÁLISE HETEROGÊNEA APLICADA NO TRATAMENTO DO CORANTE RODAMINA B – USO DE CATALISADOR DE TIO2 SOB RADIAÇÃO UV

Resumo: Os efluentes dos processos de tingimento na indústria têxtil apresentam uma complexa composição, incluindo principalmente corantes de diferente natureza química, portanto o tratamento destes tem como finalidades necessárias a remoção da cor e a degradação dos compostos orgânicos presentes. Dessa forma, este projeto teve por objetivo apresentar uma alternativa frente ao descarte de efluentes têxteis, usando como modelo o corante rodamina B, um corante resistente à fotodegradação natural. O projeto consistiu na construção de um reator fotocatalítico usando uma placa de vidro jateada e impregnada com catalisador de TiO2. Essa placa foi fixada com inclinação estabelecida. Além disso, foram utilizadas duas lâmpadas germicidas de radiação UV e potência de 15 W. Foram analisados a influência da concentração inicial, tempo de residência no reator e pH da solução para remoção de cor do corante. A descoloração da solução foi acompanhada pela medida da absorbância das amostras coletadas durante o ensaio. A eficiência do reator foi avaliada em relação à descoloração da solução. Os melhores resultados foram obtidos para uma solução de 7,80 mg L-1 a pH 5.0 foi tratada por 430 min alcançando um 97,84% de descoloração. AbstractThe effluents from the dyeing processes in the textile industry have a complex composition, including mainly dyes of different chemical nature, so the treatment of these has the necessary purposes of removing the color and the degradation of the organic compounds present. In this way, this project aimed to present an alternative to the disposal of textile effluents, using as a model the rhodamine B dye, a dye resistant to natural photodegradation. The project consisted in the construction of a photocatalytic reactor using a blasted glass plate and impregnated with TiO2 catalyst, It was fixed in a pre-established inclination, besides that, two germicide UV lamps at 15 W were employed. The influence of the initial concentration, residence time in the reactor and pH of the solution for color removal of the dye were analyzed. Solution discoloration was followed by absorbance measurement of the sample solution collected along the assay. The reactor efficiency was evaluated in relation to the discoloration of the sample solution. Best results were achieved when a solution of 7.80 mg L-1 at pH 5.0 was treated for 430 min reaching 97.84% of discoloration

Antagonistic activity of lactic acid bacteria isolated from Minas artisanal cheeses against Brucella abortus

Este estudo teve como objetivo avaliar métodos de estudo in vitro da atividade antimicrobiana de bactérias lácticas contra Brucella abortus e avaliar o efeito antagônico das mesmas sobre a viabilidade deste patógeno. Um total de 18 amostras de bactérias lácteas (Lactobacillus plantarum, n = 11; Pediococcus acidilactici, n = 1; Lactobacillus rhamnosus, n = 4; e Lactobacillus brevis, n = 2), isoladas de exemplares de Queijo Minas Artesanal produzidos em três regiões (Canastra, Campos das Vertentes e Araxá) do estado de Minas Gerais, Brasil, foram testados quanto à sua atividade antimicrobiana contra B. abortus usando três métodos: spot-on-lawn, ensaio de difusão em poço e atividade antagonista de sobrenadante de cultura. Nenhuma das cepas testadas foi capaz de inibir B. abortus nos ensaios spot-on-lawm e de difusão em poço. Os sobrenadantes produzidos pelas bactérias lácteas apresentaram pH ácido, com intensidade dependente do crescimento bacteriano e da amostra, podendo inibir o crescimento de B. abortus. Em contraste, os sobrenadantes com pH neutralizado (pH 7,0) não inibiram o crescimento de B. abortus. Os resultados mostraram que a melhor técnica para estudar o antagonismo in vitro de bactérias lácteas contra B. abortus foi a atividade antagonista de sobrenadante de cultura. O crescimento de B. abortus pode ter sido inibido pela produção de ácido.This study aimed to evaluate methods for studying the in vitro antimicrobial activity of lactic acid bacteria (LAB) against Brucella abortus and to evaluate the antagonistic effect of LAB on the viability of this pathogen. A total of 18 LAB strains (Lactobacillus plantarum, n = 11; Pediococcus acidilactici, n = 1; Lactobacillus rhamnosus, n = 4; and Lactobacillus brevis, n = 2), isolated from Minas artisanal cheeses produced in three regions (Canastra, Campos das Vertentes, and Araxá) of Minas Gerais State, Brazil, were tested for their antimicrobial activity against B. abortus using three methods: spot-on-lawn, agar well diffusion assay, and antagonistic activity of the culture supernatants. None of the tested LAB strains could inhibit B. abortus in the spot-on-lawn and agar-well diffusion assays. The supernatants produced by LAB had an acidic pH, with intensity depending on bacterial growth and strain, and could inhibit the growth of B. abortus. In contrast, pH-neutralized (pH 7.0) LAB supernatants did not suppress the growth of B. abortus. The results showed that the best technique to study the in vitro antagonism of LAB against B. abortus was the antagonistic activity of culture supernatants. The growth of B. abortus may have been inhibited by acid production

Antagonistic activity of lactic acid bacteria isolated from Minas artisanal cheeses against Brucella abortus

This study aimed to evaluate methods for studying the in vitro antimicrobial activity of lactic acid bacteria (LAB) against Brucella abortus and to evaluate the antagonistic effect of LAB on the viability of this pathogen. A total of 18 LAB strains (Lactobacillus plantarum, n = 11; Pediococcus acidilactici, n = 1; Lactobacillus rhamnosus, n = 4; and Lactobacillus brevis, n = 2), isolated from Minas artisanal cheeses produced in three regions (Canastra, Campos das Vertentes, and Araxá) of Minas Gerais State, Brazil, were tested for their antimicrobial activity against B. abortus using three methods: spot-on-lawn, agar well diffusion assay, and antagonistic activity of the culture supernatants. None of the tested LAB strains could inhibit B. abortus in the spot-on-lawn and agar-well diffusion assays. The supernatants produced by LAB had an acidic pH, with intensity depending on bacterial growth and strain, and could inhibit the growth of B. abortus. In contrast, pH-neutralized (pH 7.0) LAB supernatants did not suppress the growth of B. abortus. The results showed that the best technique to study the in vitro antagonism of LAB against B. abortus was the antagonistic activity of culture supernatants. The growth of B. abortus may have been inhibited by acid production

Overview of Ceramic Minerals Applications in Dentistry and New Perspectives: An Integrative Review

Abstract: Modern dentistry is constantly searching for new materials that meet specific clinical requirements. Among these materials, ceramics stand out for their superior properties, such as biocompatibility, and are widely used in areas such as prosthetics, dentistry, implantology, and other related specialties. The biocompatibility of ceramic materials is one of the main reasons for their numerous applications in clinical practice, providing significant benefits for the recovery and maintenance of oral health. Objective: The present study aimed to carry out a comprehensive survey of scientific productions related to the applications of ceramic materials in dentistry. Materials and Methods: The research was conducted as an integrative literature review, covering five electronic databases to ensure broad and detailed coverage of relevant studies. Results: Initially, 1329 study records were found. After applying the inclusion and exclusion criteria, 13 studies were selected for analysis. These studies addressed several facets of the clinical applications of ceramic materials, highlighting their advantages and limitations. Conclusion: Despite the significant potential of ceramic materials in the area of ​​dental health, the analysis revealed a deficit in the global literature on the approach to this specific topic. There is a clear need for further research to explore in more depth the applications of these materials, their physical and chemical characteristics, and their interactions with biological tissues, in order to optimize their use in clinical practice.Modern dentistry is constantly searching for new materials that meet specific clinical requirements. Among these materials, ceramics stand out for their superior properties, such as biocompatibility, and are widely used in areas such as prosthetics, dentistry, implantology, and other related specialties. The biocompatibility of ceramic materials is one of the main reasons for their numerous applications in clinical practice, providing significant benefits for the recovery and maintenance of oral health. Objective: The present study aimed to carry out a comprehensive survey of scientific productions related to the applications of ceramic materials in dentistry. Materials and Methods: The research was conducted as an integrative literature review, covering five electronic databases to ensure broad and detailed coverage of relevant studies. Results: Initially, 1329 study records were found. After applying the inclusion and exclusion criteria, 13 studies were selected for analysis. These studies addressed several facets of the clinical applications of ceramic materials, highlighting their advantages and limitations. Conclusion: Despite the significant potential of ceramic materials in the area of ​​dental health, the analysis revealed a deficit in the global literature on the approach to this specific topic. There is a clear need for further research to explore in more depth the applications of these materials, their physical and chemical characteristics, and their interactions with biological tissues, in order to optimize their use in clinical practice

Rationale, study design, and analysis plan of the Alveolar Recruitment for ARDS Trial (ART): Study protocol for a randomized controlled trial

Background: Acute respiratory distress syndrome (ARDS) is associated with high in-hospital mortality. Alveolar recruitment followed by ventilation at optimal titrated PEEP may reduce ventilator-induced lung injury and improve oxygenation in patients with ARDS, but the effects on mortality and other clinical outcomes remain unknown. This article reports the rationale, study design, and analysis plan of the Alveolar Recruitment for ARDS Trial (ART). Methods/Design: ART is a pragmatic, multicenter, randomized (concealed), controlled trial, which aims to determine if maximum stepwise alveolar recruitment associated with PEEP titration is able to increase 28-day survival in patients with ARDS compared to conventional treatment (ARDSNet strategy). We will enroll adult patients with ARDS of less than 72 h duration. The intervention group will receive an alveolar recruitment maneuver, with stepwise increases of PEEP achieving 45 cmH(2)O and peak pressure of 60 cmH2O, followed by ventilation with optimal PEEP titrated according to the static compliance of the respiratory system. In the control group, mechanical ventilation will follow a conventional protocol (ARDSNet). In both groups, we will use controlled volume mode with low tidal volumes (4 to 6 mL/kg of predicted body weight) and targeting plateau pressure <= 30 cmH2O. The primary outcome is 28-day survival, and the secondary outcomes are: length of ICU stay; length of hospital stay; pneumothorax requiring chest tube during first 7 days; barotrauma during first 7 days; mechanical ventilation-free days from days 1 to 28; ICU, in-hospital, and 6-month survival. ART is an event-guided trial planned to last until 520 events (deaths within 28 days) are observed. These events allow detection of a hazard ratio of 0.75, with 90% power and two-tailed type I error of 5%. All analysis will follow the intention-to-treat principle. Discussion: If the ART strategy with maximum recruitment and PEEP titration improves 28-day survival, this will represent a notable advance to the care of ARDS patients. Conversely, if the ART strategy is similar or inferior to the current evidence-based strategy (ARDSNet), this should also change current practice as many institutions routinely employ recruitment maneuvers and set PEEP levels according to some titration method.Hospital do Coracao (HCor) as part of the Program 'Hospitais de Excelencia a Servico do SUS (PROADI-SUS)'Brazilian Ministry of Healt

Immunoglobulin, glucocorticoid, or combination therapy for multisystem inflammatory syndrome in children: a propensity-weighted cohort study.

BACKGROUND: Multisystem inflammatory syndrome in children (MIS-C), a hyperinflammatory condition associated with SARS-CoV-2 infection, has emerged as a serious illness in children worldwide. Immunoglobulin or glucocorticoids, or both, are currently recommended treatments. METHODS: The Best Available Treatment Study evaluated immunomodulatory treatments for MIS-C in an international observational cohort. Analysis of the first 614 patients was previously reported. In this propensity-weighted cohort study, clinical and outcome data from children with suspected or proven MIS-C were collected onto a web-based Research Electronic Data Capture database. After excluding neonates and incomplete or duplicate records, inverse probability weighting was used to compare primary treatments with intravenous immunoglobulin, intravenous immunoglobulin plus glucocorticoids, or glucocorticoids alone, using intravenous immunoglobulin as the reference treatment. Primary outcomes were a composite of inotropic or ventilator support from the second day after treatment initiation, or death, and time to improvement on an ordinal clinical severity scale. Secondary outcomes included treatment escalation, clinical deterioration, fever, and coronary artery aneurysm occurrence and resolution. This study is registered with the ISRCTN registry, ISRCTN69546370. FINDINGS: We enrolled 2101 children (aged 0 months to 19 years) with clinically diagnosed MIS-C from 39 countries between June 14, 2020, and April 25, 2022, and, following exclusions, 2009 patients were included for analysis (median age 8·0 years [IQR 4·2-11·4], 1191 [59·3%] male and 818 [40·7%] female, and 825 [41·1%] White). 680 (33·8%) patients received primary treatment with intravenous immunoglobulin, 698 (34·7%) with intravenous immunoglobulin plus glucocorticoids, 487 (24·2%) with glucocorticoids alone; 59 (2·9%) patients received other combinations, including biologicals, and 85 (4·2%) patients received no immunomodulators. There were no significant differences between treatments for primary outcomes for the 1586 patients with complete baseline and outcome data that were considered for primary analysis. Adjusted odds ratios for ventilation, inotropic support, or death were 1·09 (95% CI 0·75-1·58; corrected p value=1·00) for intravenous immunoglobulin plus glucocorticoids and 0·93 (0·58-1·47; corrected p value=1·00) for glucocorticoids alone, versus intravenous immunoglobulin alone. Adjusted average hazard ratios for time to improvement were 1·04 (95% CI 0·91-1·20; corrected p value=1·00) for intravenous immunoglobulin plus glucocorticoids, and 0·84 (0·70-1·00; corrected p value=0·22) for glucocorticoids alone, versus intravenous immunoglobulin alone. Treatment escalation was less frequent for intravenous immunoglobulin plus glucocorticoids (OR 0·15 [95% CI 0·11-0·20]; p<0·0001) and glucocorticoids alone (0·68 [0·50-0·93]; p=0·014) versus intravenous immunoglobulin alone. Persistent fever (from day 2 onward) was less common with intravenous immunoglobulin plus glucocorticoids compared with either intravenous immunoglobulin alone (OR 0·50 [95% CI 0·38-0·67]; p<0·0001) or glucocorticoids alone (0·63 [0·45-0·88]; p=0·0058). Coronary artery aneurysm occurrence and resolution did not differ significantly between treatment groups. INTERPRETATION: Recovery rates, including occurrence and resolution of coronary artery aneurysms, were similar for primary treatment with intravenous immunoglobulin when compared to glucocorticoids or intravenous immunoglobulin plus glucocorticoids. Initial treatment with glucocorticoids appears to be a safe alternative to immunoglobulin or combined therapy, and might be advantageous in view of the cost and limited availability of intravenous immunoglobulin in many countries. FUNDING: Imperial College London, the European Union's Horizon 2020, Wellcome Trust, the Medical Research Foundation, UK National Institute for Health and Care Research, and National Institutes of Health

Archaea: potencial biotecnológico

Submitted by Alexandre Sousa ([email protected]) on 2015-11-09T16:49:10Z No. of bitstreams: 1 Biotec Cienc Desenv_30.pdf: 256940 bytes, checksum: 79b715b41243787328bc6b1c614a3e4a (MD5)Approved for entry into archive by Alexandre Sousa ([email protected]) on 2015-11-09T17:06:54Z (GMT) No. of bitstreams: 1 Biotec Cienc Desenv_30.pdf: 256940 bytes, checksum: 79b715b41243787328bc6b1c614a3e4a (MD5)Made available in DSpace on 2015-11-09T17:06:54Z (GMT). No. of bitstreams: 1 Biotec Cienc Desenv_30.pdf: 256940 bytes, checksum: 79b715b41243787328bc6b1c614a3e4a (MD5) Previous issue date: 2003Universidade Federal do Rio de Janeiro, Instituto de Ciências Biomédicas. Departamento de Bioquímica Médica. Rio de Janeiro, RJ, Brasil.Fundação Oswaldo Cruz. Instituto Nacional de Controle de Qualidade em Saúde. Departamento de Microbiologia. Rio de Janeiro, RJ, Brasil.Universidade Federal do Rio de Janeiro, Instituto de Ciências Biomédicas. Departamento de Bioquímica Médica. Rio de Janeiro, RJ, Brasil.Universidade Federal do Rio de Janeiro, Instituto de Biologia. Departamento de Biologia Marinha. Rio de Janeiro, RJ, Brasil.Universidade Federal do Rio de Janeiro, COPPE, Programa de Engenharia Química. Rio de Janeiro, RJ, Brasil.Universidade Federal do Rio de Janeiro, Instituto de Ciências Biomédicas. Departamento de Bioquímica Médica. Rio de Janeiro, RJ, Brasil.Universidade Federal do Rio de Janeiro, Instituto de Ciências Biomédicas. Departamento de Bioquímica Médica. Rio de Janeiro, RJ, Brasil

Explorando as aplicações biotecnológicas do domínio Archaea

Submitted by Alexandre Sousa ([email protected]) on 2015-12-16T10:28:15Z No. of bitstreams: 1 Braz_J_Microbiol_38_398-405.pdf: 488726 bytes, checksum: b81824bef49afc9dfa9158af9528d14d (MD5)Approved for entry into archive by Alexandre Sousa ([email protected]) on 2015-12-16T10:39:55Z (GMT) No. of bitstreams: 1 Braz_J_Microbiol_38_398-405.pdf: 488726 bytes, checksum: b81824bef49afc9dfa9158af9528d14d (MD5)Made available in DSpace on 2015-12-16T10:39:55Z (GMT). No. of bitstreams: 1 Braz_J_Microbiol_38_398-405.pdf: 488726 bytes, checksum: b81824bef49afc9dfa9158af9528d14d (MD5) Previous issue date: 2007Universidade Federal do Rio de Janeiro. Instituto de Bioquímica Médica. Rio de Janeiro, RJ, Brasil.Universidade Federal do Rio de Janeiro. Instituto de Química. Rio de Janeiro, RJ, Brasil.Fundação Oswaldo Cruz. Instituto Nacional de Controle de Qualidade em Saúde. Departamento de Microbiologia. Rio de Janeiro, RJ, Brasil.Universidade Federal do Rio de Janeiro. Instituto de Bioquímica Médica. Rio de Janeiro, RJ, Brasil.Universidade Federal do Rio de Janeiro. Instituto de Bioquímica Médica. Rio de Janeiro, RJ, Brasil.Universidade Federal do Rio de Janeiro. Instituto de Bioquímica Médica. Rio de Janeiro, RJ, Brasil.Universidade Federal do Rio de Janeiro. Instituto de Bioquímica Médica. Rio de Janeiro, RJ, Brasil.As arqueas representam uma considerável fração dos procariotos nos ecossistemas marinhos e terrestes, indicando que estes organismos devem possuir um grande impacto nos ciclos energéticos. A natureza extremofílica de muitas arqueas tem estimulado intensos esforços para compreender sua adaptação fisiológica a ambientes extremos. Suas propriedades incomus as tornam uma fonte valiosa no desenvolvimento de novos processos biotecnológicos e aplicações industriais como novos fármacos, cosméticos, suplementos nutricionais, sondas moleculares, enzimas e reagentes. Na presente mini-revisão, mostramos e discutimos algumas de suas características exclusivas correlacionando-as com seu potencial biotecnológico e aplicação industrial. Os tópicos são: características das arqueas, divisão filogenética, aplicações biotecnológicas, isolamento e cultivo de novos microrganismos, genoma e metagenoma.Archaea represent a considerable fraction of the prokaryotic world in marine and terrestrial ecosystems, indicating that organisms from this domain might have a large impact on global energy cycles. The extremophilic nature of many archaea has stimulated intense efforts to understand the physiological adaptations for living in extreme environments. Their unusual properties make them a potentially valuable resource in the development of novel biotechnological processes and industrial applications as new pharmaceuticals, cosmetics, nutritional supplements, molecular probes, enzymes, and fine chemicals. In the present mini-review, we show and discuss some exclusive characteristics of Archaea domain and the current knowledge about the biotechnological uses of the archaeal enzymes. The topics are: archaeal characteristics, phylogenetic division, biotechnological applications, isolation and cultivation of new microbes, achievements in genomics, and metagenomic

Molecular characterization of quinolone-resistant Neisseria gonorrhoeae isolates from Brazil

Submitted by Alexandre Sousa ([email protected]) on 2015-06-30T18:02:21Z No. of bitstreams: 1 J._Clin_Microbiol_49_4208-12.pdf: 288782 bytes, checksum: 0d176fd506305eeacc9f1d9e6a2e6b37 (MD5)Approved for entry into archive by Alexandre Sousa ([email protected]) on 2015-06-30T18:11:01Z (GMT) No. of bitstreams: 1 J._Clin_Microbiol_49_4208-12.pdf: 288782 bytes, checksum: 0d176fd506305eeacc9f1d9e6a2e6b37 (MD5)Approved for entry into archive by Alexandre Sousa ([email protected]) on 2015-06-30T18:34:02Z (GMT) No. of bitstreams: 1 J._Clin_Microbiol_49_4208-12.pdf: 288782 bytes, checksum: 0d176fd506305eeacc9f1d9e6a2e6b37 (MD5)Made available in DSpace on 2015-06-30T18:34:02Z (GMT). No. of bitstreams: 1 J._Clin_Microbiol_49_4208-12.pdf: 288782 bytes, checksum: 0d176fd506305eeacc9f1d9e6a2e6b37 (MD5) Previous issue date: 2011Universidade Federal do Rio de Janeiro. Instituto de Microbiologia Paulo de Góes. Departamento Microbiologia Médica. Rio de Janeiro, RJ, Brasil.Laboratório Sérgio Franco, Rio de Janeiro, RJ, Brasil.Laboratório Sérgio Franco, Rio de Janeiro, RJ, Brasil.Fundação Oswaldo Cruz. Instituto Nacional de Controle de Qualidade em Saúde. Departamento de Microbiologia. Rio de Janeiro, RJ, Brasil.Fundação Oswaldo Cruz. Instituto Nacional de Controle de Qualidade em Saúde. Departamento de Microbiologia. Rio de Janeiro, RJ, Brasil.Fundação Oswaldo Cruz. Instituto Nacional de Controle de Qualidade em Saúde. Departamento de Microbiologia. Rio de Janeiro, RJ, Brasil.Universidade Federal Fluminense. Instituto Biomédico. Niterói, RJ, Brasil.Universidade Federal do Rio de Janeiro. Instituto de Microbiologia Paulo de Góes. Departamento Microbiologia Médica. Rio de Janeiro, RJ, Brasil.Universidade Federal do Rio de Janeiro. Instituto de Microbiologia Paulo de Góes. Departamento Microbiologia Médica. Rio de Janeiro, RJ, Brasil.Universidade Federal do Rio de Janeiro. Instituto de Microbiologia Paulo de Góes. Departamento Microbiologia Médica. Rio de Janeiro, RJ, Brasil.Universidade Federal do Rio de Janeiro. Instituto de Microbiologia Paulo de Góes. Departamento Microbiologia Médica. Rio de Janeiro, RJ, Brasil.Despite the rapid spread of antibiotic resistance among gonococci worldwide, limited reports are available from Brazilian locations. In the present study, 25 quinolone-resistant Neisseria gonorrhoeae (QRNG) strains isolated in Rio de Janeiro, Brazil, were characterized by phenotypic and molecular methods, including analysis of mutations in the gyrA and parC genes. They represented 16.5% of the N. gonorrhoeae isolates obtained during a survey performed from 2006 to 2010. A trend for increasing resistance to ciprofloxacin was observed in the period investigated. The most prevalent pattern of mutation observed among QRNG isolates, Ser-91 to Phe and Asp-95 to Gly in gyrA and Ser-87 to Arg in parC, was detected in 40% of the isolates exhibiting MICs ranging from 4 to >32 mug/ml. Rare types of mutations were found in the gyrA gene (Gln-102 to His [12%] and Asp-95 to Tyr [4%]) and in the parC gene (Ser-88 to Thr [4%]). The genetic relationship of the QRNG isolates, evaluated by pulsed-field gel electrophoresis, suggested that the increase in the frequencies of the QRNG isolates in Rio de Janeiro, Brazil, may have arisen as a result of simultaneous spread of two clonal groups. The results also indicate that fluoroquinolones may no longer be used as first line antibiotics for the treatment of gonorrhea in Rio de Janeiro, and that programs for antimicrobial susceptibility surveillance of N. gonorrhoeae should also be implemented in other regions of Brazil

Aerobic physical training reduces severe asthma phenotype involving kinins pathway

Abstract: IntroductionAerobic physical training (APT) reduces eosinophilic airway inflammation, but its effects and mechanisms in severe asthma remain unknown.MethodsAn in vitro study employing key cells involved in the pathogenesis of severe asthma, such as freshly isolated human eosinophils, neutrophils, and bronchial epithelial cell lineage (BEAS-2B) and lung fibroblasts (MRC-5 cells), was conducted. Additionally, an in vivo study using male C57Bl/6 mice, including Control (Co; n = 10), Trained (Exe; n = 10), house dust mite (HDM; n = 10), and HDM + Trained (HDM + Exe; n = 10) groups, was carried out, with APT performed at moderate intensity, 5x/week, for 4 weeks.ResultsHDM and bradykinin, either alone or in combination, induced hyperactivation in human neutrophils, eosinophils, BEAS-2B, and MRC-5 cells. In contrast, IL-10, the primary anti-inflammatory molecule released during APT, inhibited these inflammatory effects, as evidenced by the suppression of numerous cytokines and reduced mRNA expression of the B1 receptor and ACE-2. The in vivo study demonstrated that APT decreased bronchoalveolar lavage levels of bradykinin, IL-1 beta, IL-4, IL-5, IL-17, IL-33, TNF-alpha, and IL-13, while increasing levels of IL-10, klotho, and IL-1RA. APT reduced the accumulation of polymorphonuclear cells, lymphocytes, and macrophages in the peribronchial space, as well as collagen fiber accumulation, epithelial thickness, and mucus accumulation. Furthermore, APT lowered the expression of the B1 receptor and ACE-2 in lung tissue and reduced bradykinin levels in the lung tissue homogenate compared to the HDM group. It also improved airway resistance, tissue resistance, and tissue damping. On a systemic level, APT reduced total leukocytes, eosinophils, neutrophils, basophils, lymphocytes, and monocytes in the blood, as well as plasma levels of IL-1 beta, IL-4, IL-5, IL-17, TNF-alpha, and IL-33, while elevating the levels of IL-10 and IL-1RA.ConclusionThese findings indicate that APT inhibits the severe asthma phenotype by targeting kinin signaling