MUSICOTERAPIA: DA PRESERVAÇÃO À RECUPERAÇÃO DA SAÚDE

A musicoterapia e a utilizacao da musica em um contexto clinico, educacional e social para prevencao e apoio a problemas de saude mental, promovendo qualidade de vida e bem estar. O emprego de sons e melodias tem o intuito de aliviar algum sintoma e tratar doenças, podendo ser empregada em adultos, crianças e idosos. Portanto, o objetivo deste projeto é proporcionar, aos internos da Santa Casa de Misericordia e do Asilo Sao Vicente de Paulo de Jacarezinho/Paraná, entretenimento, distração, socialização e melhora do bem- estar, prevenindo e aliviando sintomas relacionados as doencas, contribuindo para uma melhor qualidade de vida. São realizadas sessões de duas horas de musicoterapia com instrumento musical e canto, uma vez por semana, em cada local, além de reuniões semanais para preparação das sessões. Acredita-se que o projeto de musicoterapia tem alcançado os seus objetivos, principalmente no que tange a melhora do cognitivo e qualidade de vida dos internos e desenvolvimento de um espírito humanista nos universitários. Esse fato pode ser observado pelos depoimentos de responsáveis pelas instituições, internos e acompanhantes, além da observação da efetiva participa

Pyrosequencing-based analysis reveals a novel capsular gene cluster in a KPC-producing Klebsiella pneumoniae clinical isolate identified in Brazil

Background: An important virulence factor of Klebsiella pneumoniae is the production of capsular polysaccharide (CPS), a thick mucus layer that allows for evasion of the host's defense and creates a barrier against antibacterial peptides. CPS production is driven mostly by the expression of genes located in a locus called cps, and the resulting structure is used to distinguish between different serotypes (K types). in this study, we report the unique genetic organization of the cps cluster from K. pneumoniae Kp13, a clinical isolate recovered during a large outbreak of nosocomial infections that occurred in a Brazilian teaching hospital.Results: A pyrosequencing-based approach showed that the cps region of Kp13 (cps(Kp13)) is 26.4 kbp in length and contains genes common, although not universal, to other strains, such as the rm/BADC operon that codes for L-rhamnose synthesis. cpsKp13 also presents some unique features, like the inversion of the wzy gene and a unique repertoire of glycosyltransferases. in silico comparison of cps(Kp13) RFLP pattern with 102 previously published cps PCR-RFLP patterns showed that cpsKp13 is distinct from the C patterns of all other K serotypes. Furthermore, in vitro serotyping showed only a weak reaction with capsular types K9 and K34. We confirm that K9 cps shares common genes with cps(Kp13) such as the rm/BADC operon, but lacks features like uge and Kp13-specific glycosyltransferases, while K34 capsules contain three of the five sugars that potentially form the Kp13 CPS.Conclusions: We report the first description of a cps cluster from a Brazilian clinical isolate of a KPC-producing K. pneumoniae. the gathered data including K-serotyping support that Kp13's K-antigen belongs to a novel capsular serotype. the CPS of Kp13 probably includes L-rhamnose and D-galacturonate in its structure, among other residues. Because genes involved in L-rhamnose biosynthesis are absent in humans, this pathway may represent potential targets for the development of antimicrobial agents. Studying the capsular serotypes of clinical isolates is of great importance for further development of vaccines and/or novel therapeutic agents. the distribution of K-types among multidrug-resistant isolates is unknown, but our findings may encourage scientists to perform K-antigen typing of KPC-producing strains worldwide.LNCC, Rio de Janeiro, BrazilUniv Fed Rio de Janeiro, Inst Microbiol Paulo de Goes, Rio de Janeiro, BrazilUniv Estadual Londrina, Dept Patol Clin Anal Clin & Toxicol, Londrina, BrazilUniversidade Federal de São Paulo, Lab ALERTA, Div Doencas Infecciosas, São Paulo, BrazilUniversidade Federal de São Paulo, Lab ALERTA, Div Doencas Infecciosas, São Paulo, BrazilWeb of Scienc

Mecanismos Disciplinadores da Gestão de Custos Interorganizacionais e Economia dos Custos de Transação: um ensaio teórico

This article aimed to verify whether the disciplining mechanisms present in the conceptual structure of the Interorganizational Cost Management (ICM) are related to the governance mechanisms established through the Transaction Cost Economics (TCE), as tools to safeguard contractual relationships. The research was developed through a theoretical essay, based on bibliographic research. Its main contribution to the literature is the identification of relationships between consolidated TCE mechanisms, which aims to verify the way the transactions occur and the recent ICM mechanisms, aimed at optimizing the return of the value chain beyond the limits of the company through cost management. In conclusion, it was verified that, despite the recent literature on ICM, the disciplining mechanisms used by this Strategic Cost Management artifact are in line with the governance mechanisms used by the TCE, in relation and synergy with the different mechanisms analyzed.El presente artículo buscó verificar si los mecanismos disciplinarios, presentes en la estructura conceptual de la Gestión de Costos Interorganizacionales (GCI), están relacionados a los mecanismos de gobernanza previstos por la Economía de los Costos de Transacción (ECT), en la condición de instrumentos para salvaguardar relaciones contractuales. El trabajo fue desarrollado por medio de un ensayo teórico, con base en investigación bibliográfica, teniendo como contribución principal para la literatura, la identificación de la existencia de relacionamientos entre mecanismos ya consolidados de la ECT, la cual busca verificar la forma como ocurren las transacciones, y los mecanismos recientes de la GCI, que busca, por medio de la gestión de costos, la optimización del retorno total de la cadena de valor más allá de los límites de la propia empresa. Como conclusión del trabajo, se constató que, aun siendo reciente la literatura acerca de la GCI, los mecanismos disciplinarios utilizados por este artefacto de la Gestión Estratégica de Costos convergen hacia los mecanismos de gobernanza utilizados por la ECT, al presentar relación y sinergia en diferentes mecanismos analizados.O presente artigo buscou verificar se os mecanismos disciplinadores presentes na estrutura conceitual da Gestão de Custos Interorganizacionais (GCI) estão relacionados aos mecanismos de governança previstos pela Economia dos Custos de Transação (ECT), enquanto instrumentos para salvaguardar relações contratuais. O trabalho foi desenvolvido por meio de um ensaio teórico, com base em pesquisa bibliográfica, tendo como contribuição principal para a literatura, a identificação da existência de relacionamentos entre mecanismos já consolidados da ECT, a qual busca verificar a forma como ocorrem as transações, e os mecanismos recentes da GCI, que busca por meio do gerenciamento de custos, a otimização do retorno total da cadeia de valor além dos limites da própria empresa. Como conclusão do trabalho constatou-se que, mesmo sendo recente a literatura acerca da GCI, os mecanismos disciplinadores utilizados por este artefato da Gestão Estratégica de Custos vão ao encontro dos mecanismos de governança utilizados pela ECT, ao apresentarem relação e sinergia em diversos dos mecanismos analisados

Origins of the Xylella fastidiosa Prophage-Like Regions and Their Impact in Genome Differentiation

Xylella fastidiosa is a Gram negative plant pathogen causing many economically important diseases, and analyses of completely sequenced X. fastidiosa genome strains allowed the identification of many prophage-like elements and possibly phage remnants, accounting for up to 15% of the genome composition. To better evaluate the recent evolution of the X. fastidiosa chromosome backbone among distinct pathovars, the number and location of prophage-like regions on two finished genomes (9a5c and Temecula1), and in two candidate molecules (Ann1 and Dixon) were assessed. Based on comparative best bidirectional hit analyses, the majority (51%) of the predicted genes in the X. fastidiosa prophage-like regions are related to structural phage genes belonging to the Siphoviridae family. Electron micrograph reveals the existence of putative viral particles with similar morphology to lambda phages in the bacterial cell in planta. Moreover, analysis of microarray data indicates that 9a5c strain cultivated under stress conditions presents enhanced expression of phage anti-repressor genes, suggesting switches from lysogenic to lytic cycle of phages under stress-induced situations. Furthermore, virulence-associated proteins and toxins are found within these prophage-like elements, thus suggesting an important role in host adaptation. Finally, clustering analyses of phage integrase genes based on multiple alignment patterns reveal they group in five lineages, all possessing a tyrosine recombinase catalytic domain, and phylogenetically close to other integrases found in phages that are genetic mosaics and able to perform generalized and specialized transduction. Integration sites and tRNA association is also evidenced. In summary, we present comparative and experimental evidence supporting the association and contribution of phage activity on the differentiation of Xylella genomes

Effects of a multidisciplinary program on autonomic modulation in overweight or obese children and adolescents

Purpose: this study evaluated the effects of a multidisciplinary program on autonomic modulation in overweight or obese children and adolescents. Methods: fi fteen individuals with 10,93±2,28 years were submitted to autonomic evaluation using heart rate variability before and after a program based on physical exercises for three months, three times a week, 60 minutes per session and psychological and nutritional counseling. Data was analyzed using the paired t-test or Wilcoxon test and the level of signifi cance was set at P < 0.05. Results: multidisciplinary program was able to promote benefi ts in autonomic modulation observed thought temporal (SDNN: 39,96 ± 10,33 x 49,44 ± 12,31; p = 0,019; rMSSD: 28,97 ± 11,50 x 37,26 ± 11,17; p = 0,018), spectral (LFnu: 64,49 ± 12,21 x 56,74 ± 11,18; p = 0,014; HFnu: 35,50 ± 12,21 x 43,25 ± 11,18; p = 0,014) and geometrical (SD1: 20,51 ± 8,13 x 26,36 ± 7,90; p = 0,018 SD2: 52,31 ± 13,04 x 64,58 ± 16,33; p = 0,031) indices in overweigh and obese children and adolescents. Also, after the intervention there was a reduction on resting heart rate (88,53 ± 9,24 x 83,09 ± 7,93; p = 0,023) and there were changes in the classifi cation of overweigh 26,67% x 46,66% and obesity 73,33% x 53,33%, although not significant changes in body mass index. Conclusions: exercise program together with nutritional and psychological counseling is able to promote benefi ts in autonomic modulation in children and adolescents who are overweight or obese

The Genome of Anopheles darlingi, the main neotropical Malaria vector

Anopheles darlingi is the principal neotropical malaria vector, responsible for more than a million cases of malaria per year on the American continent. Anopheles darlingi diverged from the African and Asian malaria vectors ∼100 million years ago (mya) and successfully adapted to the New World environment. Here we present an annotated reference A. darlingi genome, sequenced from a wild population of males and females collected in the Brazilian Amazon. A total of 10 481 predicted protein-coding genes were annotated, 72% of which have their closest counterpart in Anopheles gambiae and 21% have highest similarity with other mosquito species. In spite of a long period of divergent evolution, conserved gene synteny was observed between A. darlingi and A. gambiae. More than 10 million single nucleotide polymorphisms and short indels with potential use as genetic markers were identified. Transposable elements correspond to 2.3% of the A. darlingi genome. Genes associated with hematophagy, immunity and insecticide resistance, directly involved in vector–human and vector–parasite interactions, were identified and discussed. This study represents the first effort to sequence the genome of a neotropical malaria vector, and opens a new window through which we can contemplate the evolutionary history of anopheline mosquitoes. It also provides valuable information that may lead to novel strategies to reduce malaria transmission on the South American continent. The A. darlingi genome is accessible a