13 research outputs found
A New Architecture for DNAâTemplated Synthesis in Which Abasic Sites Protect Reactants from Degradation
The synthesis of artificial sequenceâdefined polymers that match and extend the functionality of proteins is an important goal in materials science. One way of achieving this is to program a sequence of chemical reactions between precursor building blocks by means of attached oligonucleotide adapters. However, hydrolysis of the reactive building blocks has so far limited the length and yield of product that can be obtained using DNAâtemplated reactions. Here, we report an architecture for DNAâtemplated synthesis in which reactants are tethered at internal abasic sites on opposite strands of a DNA duplex. We show that an abasic site within a DNA duplex can protect a nearby thioester from degradation, significantly increasing the yield of a DNAâtemplated reaction. This protective effect has the potential to overcome the challenges associated with programmable, sequenceâcontrolled synthesis of long nonânatural polymers by extending the lifetime of the reactive building blocks
A New Architecture for DNAâTemplated Synthesis in Which Abasic Sites Protect Reactants from Degradation
The synthesis of artificial sequenceâdefined polymers that match and extend the functionality of proteins is an important goal in materials science. One way of achieving this is to program a sequence of chemical reactions between precursor building blocks by means of attached oligonucleotide adapters. However, hydrolysis of the reactive building blocks has so far limited the length and yield of product that can be obtained using DNAâtemplated reactions. Here, we report an architecture for DNAâtemplated synthesis in which reactants are tethered at internal abasic sites on opposite strands of a DNA duplex. We show that an abasic site within a DNA duplex can protect a nearby thioester from degradation, significantly increasing the yield of a DNAâtemplated reaction. This protective effect has the potential to overcome the challenges associated with programmable, sequenceâcontrolled synthesis of long nonânatural polymers by extending the lifetime of the reactive building blocks
Towards Customary Legal Empowerment
Rule of Law and Development: Formation, Implementation and Improvement of Law and Governance in Developing Countrie