623 research outputs found
Effect of silicic acid and other silicon compounds on fungal growth in oligotrophic and nutrient-rich media
Mycelium grew from a spore-mycelial inoculum of Aspergillus oryzae added to ultra-pure water (upw) containing silicon compounds, but did not grow in upw alone. Growth of other fungi also occurred in upw only when silicon compounds were added. Increased growth of A. oryzae, and other fungi, also followed the addition of silicic acid and other silicon compounds to Czapek Dox. Aspergillus oryzae solubilized silicon compounds in both upw and nutrient-rich media. Although interactions between microorganisms and silicon have been generally neglected, the results show that silicon compounds can increase fungal growth under both oligotrophic and nutrient-rich conditions
Diagnosis of vertebral fractures in children: is a simplified algorithm-based qualitative technique reliable?
Background Identification of osteoporotic vertebral fractures
allows treatment opportunity reducing future risk. There is no
agreed standardised method for diagnosing paediatric vertebral
fractures.
Objective To evaluate the precision of a modified adult
algorithm-based qualitative (ABQ) technique, applicable to
children with primary or secondary osteoporosis.
Materials and methods Three radiologists independently
assessed lateral spine radiographs of 50 children with
suspected reduction in bone mineral density using a modified
ABQ scoring system and following simplification to include
only clinically relevant parameters, a simplified ABQ score. A
final consensus of all observers using simplified ABQ was
performed as a reference standard for fracture characterisation.
Kappa was calculated for interobserver agreement of the
components of both scoring systems and intraobserver agreement
of simplified ABQ based on a second read of 29 randomly
selected images.
Results Interobserver Kappa for modified ABQ scoring for
fracture detection, severity and shape ranged from 0.34 to
0.49 Kappa for abnormal endplate and position assessment
was 0.27 to 0.38. Inter- and intraobserver Kappa for simplified
ABQ scoring for fracture detection and grade ranged from
0.37 to 0.46 and 0.45 to 0.56, respectively. Inter- and
intraobserver Kappa for affected endplate ranged from 0.31
to 0.41 and 0.45 to 0.51, respectively. Subjectively, observersâ
felt simplified ABQ was easier and less time-consuming.
Conclusion Observer reliability of modified and simplified
ABQ was similar, with slight to moderate agreement for fracture
detection and grade/severity. Due to subjective preference
for simplified ABQ, we suggest its use as a semi-objective
measure of diagnosing paediatric vertebral fracture
Diagnostic accuracy of DXA compared to conventional spine radiographs for the detection of vertebral fractures in children
Objectives
In children, radiography is performed to diagnose vertebral fractures and dual energy x-ray absorptiometry (DXA) to
assess bone density. In adults, DXA assesses both. We aimed to establish whether
DXA can replace spine radiographs in assessment of paediatric vertebral fractures.
Methods
Prospectively, lateral spine radiographs and lateral spine DXA of 250 children
performed on the same day were independently scored by three radiologists using
the simplified algorithm based qualitative technique and blinded to results of the
other modality. Consensus radiograph read and second read of 100 random images
were performed. Diagnostic accuracy, inter/intraobserver and intermodality
agreements, patient/carer experience and radiation dose were assessed.
Results
Average sensitivity and specificity (95% confidence interval) in diagnosing one or
more vertebral fractures requiring treatment was 70% (58%-82%) and 97% (94%-
100%) respectively for DXA and 74% (55%-93%) and 96% (95%-98%) for
radiographs. Fleissâ kappa for interobserver and average kappa for intraobserver
reliability were 0.371 and 0.631 respectively for DXA and 0.418 and 0.621 for
radiographs. Average effective dose was 41.9”Sv for DXA and 232.7”Sv for
radiographs. Image quality was similar.
Conclusion
Given comparable image quality and non-inferior diagnostic accuracy, lateral spine
DXA should replace conventional radiographs for assessment of vertebral fractures
in children
Role of lepton flavor violating (LFV) muon decay in Seesaw model and LSND
The aim of the work is to study LFV in a newly proposed Seesaw model of
neutrino mass and to see whether it could explain LSND excess. The motivation
of this Seesaw model was that there was no new physics beyond the TeV scale. By
studying \mu \to 3e in this model, it is shown that the upper bound on the
branching ratio requires Higgs mass m_{h} of a new scalar doublet with lepton
number L=-1 needed in the model has to be about 9 TeV. The predicted branching
ratio for \mu \to e\nu_{l}\bar{\nu}_{l} is too small to explain the LSND. PACS:
11.30.Hv, 14.60.PqComment: 05 pages, three figures, the version to appear in PR
Development and characterization of a microfluidic model of the tumour microenvironment
The physical microenvironment of tumours is characterized by heterotypic cell interactions and physiological gradients of nutrients, waste products and oxygen. This tumour microenvironment has a major impact on the biology of cancer cells and their response to chemotherapeutic agents. Despite this, most in vitro cancer research still relies primarily on cells grown in 2D and in isolation in nutrient- and oxygen-rich conditions. Here, a microfluidic device is presented that is easy to use and enables modelling and study of the tumour microenvironment in real-time. The versatility of this microfluidic platform allows for different aspects of the microenvironment to be monitored and dissected. This is exemplified here by real-time profiling of oxygen and glucose concentrations inside the device as well as effects on cell proliferation and growth, ROS generation and apoptosis. Heterotypic cell interactions were also studied. The device provides a live âwindowâ into the microenvironment and could be used to study cancer cells for which it is difficult to generate tumour spheroids. Another major application of the device is the study of effects of the microenvironment on cellular drug responses. Some data is presented for this indicating the deviceâs potential to enable more physiological in vitro drug screening
Predictive Value of Ov16 Antibody Prevalence in Different Subpopulations for Elimination of African Onchocerciasis
The World Health Organization currently recommends assessing elimination of onchocerciasis by testing whether Ov16 antibody prevalence in children aged 0-9 years is below 0.1%. However, the certainty of evidence for this recommendation is considered to be low. We used the established ONCHOSIM model to investigate the predictive value of different Ov16-antibody prevalence thresholds in various age groups for elimination of onchocerciasis in a variety of endemic settings and for various mass drug administration scenarios. According to our simulations, the predictive value of Ov16 antibody prevalence for elimination depends highly on the precontrol epidemiologic situation, history of mass drug administration, the age group that is sampled, and the chosen Ov16-antibody prevalence threshold. The Ov16 antibody prevalence in children aged 5-14 years performs best in predicting elimination. Appropriate threshold values for this age group start at 2.0% for very highly endemic areas; for lower-endemic areas, even higher threshold values are safe to use. Guidelines can be improved by sampling school-aged children, which also is operationally more feasible than targeting children under age 10 years. The use of higher threshold values allows sampling of substantially fewer children. Further improvement can be achieved by taking a differentiated sampling approach based on precontrol endemicity
Aquatic food security:insights into challenges and solutions from an analysis of interactions between fisheries, aquaculture, food safety, human health, fish and human welfare, economy and environment
Fisheries and aquaculture production, imports, exports and equitability of distribution determine the supply of aquatic food to people. Aquatic food security is achieved when a food supply is sufficient, safe, sustainable, shockproof and sound: sufficient, to meet needs and preferences of people; safe, to provide nutritional benefit while posing minimal health risks; sustainable, to provide food now and for future generations; shock-proof, to provide resilience to shocks in production systems and supply chains; and sound, to meet legal and ethical standards for welfare of animals, people and environment. Here, we present an integrated assessment of these elements of the aquatic food system in the United Kingdom, a system linked to dynamic global networks of producers, processors and markets. Our assessment addresses sufficiency of supply from aquaculture, fisheries and trade; safety of supply given biological, chemical and radiation hazards; social, economic and environmental sustainability of production systems and supply chains; system resilience to social, economic and environmental shocks; welfare of fish, people and environment; and the authenticity of food. Conventionally, these aspects of the food system are not assessed collectively, so information supporting our assessment is widely dispersed. Our assessment reveals trade-offs and challenges in the food system that are easily overlooked in sectoral analyses of fisheries, aquaculture, health, medicine, human and fish welfare, safety and environment. We highlight potential benefits of an integrated, systematic and ongoing process to assess security of the aquatic food system and to predict impacts of social, economic and environmental change on food supply and demand
The differential effects of concurrent planning practice elements on reunification and adoption
Objective: The child welfare practice of concurrent planning attempts to shorten children\u27s stays in foster care. There is very little quantitative research on concurrent planning\u27s effects. This study examines the influence of concurrent planning practice elements (reunification prognosis, concurrent plan, full disclosure, and discussion of voluntary relinquishment) on reunification and adoption. Method: Using a sample of 885 children, an observational design, and statistical controls, children who received concurrent planning elements were compared to those who did not. Results: Findings show discussion of voluntary relinquishment to be positively associated with adoption and full disclosure to be negatively associated with reunification. Conclusions: Concurrent planning\u27s benefits may require more intensive services to be fully realized. Care should be taken to ensure activities achieve their intended effects
Low-mass pre--main-sequence stars in the Magellanic Clouds
[Abridged] The stellar Initial Mass Function (IMF) suggests that sub-solar
stars form in very large numbers. Most attractive places for catching low-mass
star formation in the act are young stellar clusters and associations, still
(half-)embedded in star-forming regions. The low-mass stars in such regions are
still in their pre--main-sequence (PMS) evolutionary phase. The peculiar nature
of these objects and the contamination of their samples by the evolved
populations of the Galactic disk impose demanding observational techniques for
the detection of complete numbers of PMS stars in the Milky Way. The Magellanic
Clouds, the companion galaxies to our own, demonstrate an exceptional star
formation activity. The low extinction and stellar field contamination in
star-forming regions of these galaxies imply a more efficient detection of
low-mass PMS stars than in the Milky Way, but their distance from us make the
application of special detection techniques unfeasible. Nonetheless, imaging
with the Hubble Space Telescope yield the discovery of solar and sub-solar PMS
stars in the Magellanic Clouds from photometry alone. Unprecedented numbers of
such objects are identified as the low-mass stellar content of their
star-forming regions, changing completely our picture of young stellar systems
outside the Milky Way, and extending the extragalactic stellar IMF below the
persisting threshold of a few solar masses. This review presents the recent
developments in the investigation of PMS stars in the Magellanic Clouds, with
special focus on the limitations by single-epoch photometry that can only be
circumvented by the detailed study of the observable behavior of these stars in
the color-magnitude diagram. The achieved characterization of the low-mass PMS
stars in the Magellanic Clouds allowed thus a more comprehensive understanding
of the star formation process in our neighboring galaxies.Comment: Review paper, 26 pages (in LaTeX style for Springer journals), 4
figures. Accepted for publication in Space Science Review
Jupiter's temperature structure: a reassessment of the voyager radio occultation results
Stars and planetary system
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