PIGMENTED BOWEN’S DISEASE MIMICKING MELANOMA CLINICALLY AND DERMOSCOPICALLY

Uma variedade de lesões cutâneas pode simular melanoma, tais como nevos melanocíticos, lentigos, ceratose seborreica, nevo azul, carcinoma basocelular pigmentado e dermatofibromas. Relata-se um caso clínico raro de uma paciente do sexo feminino que apresentou lesão em coxa esquerda clínica e dermatoscopicamente compatível com melanoma. A paciente foi submetida à exérese da lesão e o histopatológico diagnosticou doença de Bowen pigmentada. O objetivo é alertar que a doença de Bowen pigmentada, uma forma rara de carcinoma espinocelular in situ, também deve ser lembrada como diagnóstico diferencial de melanoma maligno.A variety of cutaneous lesions can mimic melanoma, such as melanocytic nevi, lentigines, seborrheic keratosis, blue nevi, pigmented basal cell carcinomas and dermatofibromas. This report describes a rare clinical case of a female patient who presented a lesion on the left thigh, which was clinically and dermoscopically compatible with melanoma. The patient underwent excision of the lesion, and histopathology confirmed a diagnosis of pigmented Bowen’s disease. The purpose of this report is to draw attention to the fact that pigmented Bowen’s disease, a rare form of squamous cell carcinoma in situ, should also be considered as a differential diagnosis of malignant melanoma

Comparação entre as técnicas de esfregaço linear e citocentrifugação para avaliação citológica do lavado broncoalveolar equino

The bronchoalveolar lavage (BAL) is a sensitive method to diagnose diseases of the distal portion of the lower respiratory tract and has been broadly used by numerous researchers. Cytocentrifugation is the choice cytological preparation technique, but demands specific and costly equipment. Therefore, the present paper intends to verify the applicability of the linear smear technique to evaluate BAL samples. For this, BAL samples of 30 equines were used and the cytological preparations were done by cytocentrifugation and linear smear techniques. All glass microscope slides were fixed and stained with Giemsa for the differential cell count. Regarding the effect of the preparation technique on differential counts, no significant difference in any cell type was found. The linear smear is a reliable alternative and can be recommended as a substitution to cytocentrifugation.O lavado broncoalveolar (LBA) é um método sensível para diagnosticar doenças do trato respiratório posterior e vem sendo utilizado por diversos esquisadores. A citocentrifugação, técnica de escolha para processar amostras citológicas de LBA, exige equipamentos específicos e caros. Por isso, este trabalho verificou a aplicabilidade da técnica de esfregaço linear para avaliação citológica do LBA. Foram utilizadas amostras de LBA de 30 equinos adultos. As preparações citológicas foram realizadas tanto por citocentrifugação quanto por esfregaço linear. Todas as lâminas foram fixadas e coradas com Giemsa para realização da contagem celular diferencial. Não foram encontradas alterações morfológicas significativas e nem diferenças estatísticas entre nenhum dos tipos celulares processados pelos dois métodos, o que permite afirmar que o método de esfregaço linear é uma alternativa segura para avaliação morfológica celular do LBA de equinos, podendo ser utilizado no lugar da citocentrifugação quando esta não estiver disponível

Evaluation of bronchoalveolar fluid of horses with inflammatory airway disease

Objetivou-se caracterizar o perfil citológico broncoalveoar de equinos de policiamento portadores assintomáticos de Doença Inflamatória de Vias Aéreas (DIVA). Utilizaram-se 17 equinos adultos, machos e fêmeas, com idade entre 11 e 24 anos. Os animais que constituíram o grupo controle (oito) apresentaram-se normais ao exame físico, à endoscopia e à mensuração da diferença máxima de pressão intrapleural. No lavado broncoalveolar a contagem deveria ser de até 4%, 0,7%, 3,3% para neutrófilos, eosinófilos e células epiteliais e entre 17,0 e 56,7%, 29 e 75,7%, 0,7 e 13,7% para linfócitos, macrófagos e mastócitos, respectivamente. O grupo considerado doente (nove animais) apresentou achados compatíveis com afecções do trato respiratório posterior, pelo menos à endoscopia, além de uma contagem de neutrófilos maior que 5% e menor que 25%. O quadro assintomático de doença inflamatória das vias aéreas caracterizou-se por infiltrado neutrofílico, discreta redução no número de macrófagos, aumento no número de macrófagos espumosos, discreto infiltrado eosinofílico e aumento marcante no número de células epiteliais. Em face do caráter assintomático dessa enfermidade nos equinos de policiamento, a utilização do LBA como método de diagnóstico e acompanhamento é fundamental, pois permite reconhecer, tratar e determinar uma utilização mais racional desses animais, de modo a melhorar a qualidade de vida e prolongar a utilização dos mesmos em serviço.The aim of this study was to characterize the cytology profile of bronchoalveolar lavage from police horses with asymptomatic Inflammatory Airway Disease (IAD). Seventeen adult horses, males and females, with ages ranging from 11 to 24 years old were utilized. The control group (eight horses) presented vital signs within the physiologic range, normal pulmonary percussion and auscultation, no abnormalities at respiratory endoscopy and at the measurement of the maximal intra-pleural pressure difference. The cell count of bronchoalveolar lavage (BAL) should be up to 4%, 0.7%, 3.3% to neutrophil, eosinophil and epithelial cell, and ranging of 17.0 to 56.7%, 29 to 75.7%, 0.7 to 13.7% to lymphocytes, macrophages and mast cell, respectively. The diseased group (nine animals) presented findings compatible with lower airway disease, at least during endoscopy and showed a neutrophil counts above 5% and below 25%. Asymptomatic IAD was characterized by neutrophilic infiltrate, discrete reduction in macrophage count with increased number of foamy macrophages and discrete eosinophilic infiltrate and marked increase in the number of epithelial cells. Due to the asymptomatic character of IAD in police horses, BAL is the preferred diagnostic and follow-up method since it allows recognition of the disease, evaluation of treatment and helps the implementation of a graded and rational work regime that aims at improving welfare of those animals as well as extending their usage as patrolling horses

Antibacterial and antioxidant activities of Derriobtusone A isolated from Lonchocarpus obtusus

This study evaluated the effect of derriobtusone A, a flavonoid isolated from Lonchocarpus obtusus, on two important pathogenic bacteria, Staphylococcus aureus and Escherichia coli, as well as its antioxidant activity and toxicity. Planktonic growth assays were performed, and the inhibition of biofilm formation was evaluated. In addition, antioxidant activity was assessed by DPPH radical scavenging assay, ferrous ion chelating assay, ferric-reducing antioxidant power assay, and β-carotene bleaching assay. Toxicity was evaluated by the brine shrimp lethality test. Results showed that derriobtusone A completely inhibited the planktonic growth of S. aureus at 250 and 500 μg/mL; however, it did not have the same activity on E. coli. Derriobtusone A reduced the biomass and colony-forming unit (cfu) of S. aureus biofilm at concentrations of 250 and 500 μg/mL. In various concentrations, it reduced the biofilm biomass of E. coli, and, in all concentrations, it weakly reduced the cfu. Derriobtusone A showed highly efficient antioxidant ability in scavenging DPPH radical and inhibiting β-carotene oxidation. The compound showed no lethality to Artemia sp. nauplii. In conclusion, derriobtusone A may be an effective molecule against S. aureus and its biofilm, as well as a potential antioxidant compound with no toxicity.This study was supported by CAPES (Brazil) through the BEX NT 2052/11NT3 Project and by IBB-CEB and FCT (Portugal) and European Community Fund FEDER, through Program COMPETE, in the ambit of Project PTDC/SAU-ESA/646091/2006/FCOMP-01-0124-FEDER-007480. Otilia Deusdenia Loiola Pessoa, Benildo Sousa Cavada, and Edson Holanda Teixeira are Senior Fellows of CNPq. Mr. David Martin helped with the English editing of the paper

Pervasive gaps in Amazonian ecological research

Biodiversity loss is one of the main challenges of our time,1,2 and attempts to address it require a clear un derstanding of how ecological communities respond to environmental change across time and space.3,4 While the increasing availability of global databases on ecological communities has advanced our knowledge of biodiversity sensitivity to environmental changes,5–7 vast areas of the tropics remain understudied.8–11 In the American tropics, Amazonia stands out as the world’s most diverse rainforest and the primary source of Neotropical biodiversity,12 but it remains among the least known forests in America and is often underrepre sented in biodiversity databases.13–15 To worsen this situation, human-induced modifications16,17 may elim inate pieces of the Amazon’s biodiversity puzzle before we can use them to understand how ecological com munities are responding. To increase generalization and applicability of biodiversity knowledge,18,19 it is thus crucial to reduce biases in ecological research, particularly in regions projected to face the most pronounced environmental changes. We integrate ecological community metadata of 7,694 sampling sites for multiple or ganism groups in a machine learning model framework to map the research probability across the Brazilian Amazonia, while identifying the region’s vulnerability to environmental change. 15%–18% of the most ne glected areas in ecological research are expected to experience severe climate or land use changes by 2050. This means that unless we take immediate action, we will not be able to establish their current status, much less monitor how it is changing and what is being lostinfo:eu-repo/semantics/publishedVersio

Updated cardiovascular prevention guideline of the Brazilian Society of Cardiology: 2019

Sem informação113478788

Pervasive gaps in Amazonian ecological research

Biodiversity loss is one of the main challenges of our time,1,2 and attempts to address it require a clear understanding of how ecological communities respond to environmental change across time and space.3,4 While the increasing availability of global databases on ecological communities has advanced our knowledge of biodiversity sensitivity to environmental changes,5,6,7 vast areas of the tropics remain understudied.8,9,10,11 In the American tropics, Amazonia stands out as the world's most diverse rainforest and the primary source of Neotropical biodiversity,12 but it remains among the least known forests in America and is often underrepresented in biodiversity databases.13,14,15 To worsen this situation, human-induced modifications16,17 may eliminate pieces of the Amazon's biodiversity puzzle before we can use them to understand how ecological communities are responding. To increase generalization and applicability of biodiversity knowledge,18,19 it is thus crucial to reduce biases in ecological research, particularly in regions projected to face the most pronounced environmental changes. We integrate ecological community metadata of 7,694 sampling sites for multiple organism groups in a machine learning model framework to map the research probability across the Brazilian Amazonia, while identifying the region's vulnerability to environmental change. 15%–18% of the most neglected areas in ecological research are expected to experience severe climate or land use changes by 2050. This means that unless we take immediate action, we will not be able to establish their current status, much less monitor how it is changing and what is being lost