Income segregation in The Netherlands - trends and analysis

Dutch society does not accept high levels of income segregation. This tendency has repeatedly been revealed in public opinion surveys. Dutch government intervenes on different levels trying to mix low and high income groups both between cities and suburbs, as well as within city neighborhoods. The question is to what extent the assumptions on actual concentration and differentiation of different income groups hold true if compared to real figures. This paper publishes income data at 500 by 500 meter cells, showing significant spatial patterns of distribution and growth of low and high income groups. Remarkably, high income groups appear to be more segregated than low income groups. All Dutch central city areas have regained high income groups in the period 1995-2000, while the reverse happens in all other city neighborhoods. Despite those tendencies, large parts of the cities have a mixed composition of low, medium and high income groups.

Spatial Scenarios and the Lisbon Strategy

The European Union is changing significantly. It has recently been enlarged by 10 new Member States, decision-making about the European Constitution is going on, and it has set itself the target of becoming the most competitive economy in the world (Lisbon Strategy). The scenarios explore in an integrated way the territorial impacts of future changes in the economy, transport, rural development, environment, and other policy fields. They do not only concentrate on the territorial impacts of the trends and driving forces but also of the relevant EU Policies. The scenarios throw a light on the effectiveness of EU Policies and the synergies as well as conflicts they generate. By doing this they provide important insights in the conditions under which these trends, driving forces and policies will favour a balanced and polycentric territory and territorial cohesion within the enlarging European Union. These insights are relevant for recommendations on possible adjustments and/or changes of EU Policies.

Income segregation in The Netherlands - trends and analysis

Dutch society does not accept high levels of income segregation. This tendency has repeatedly been revealed in public opinion surveys. Dutch government intervenes on different levels trying to mix low and high income groups both between cities and suburbs, as well as within city neighborhoods. The question is to what extent the assumptions on actual concentration and differentiation of different income groups hold true if compared to real figures. This paper publishes income data at 500 by 500 meter cells, showing significant spatial patterns of distribution and growth of low and high income groups. Remarkably, high income groups appear to be more segregated than low income groups. All Dutch central city areas have regained high income groups in the period 1995-2000, while the reverse happens in all other city neighborhoods. Despite those tendencies, large parts of the cities have a mixed composition of low, medium and high income groups

Spatial Scenarios and the Lisbon Strategy

The European Union is changing significantly. It has recently been enlarged by 10 new Member States, decision-making about the European Constitution is going on, and it has set itself the target of becoming the most competitive economy in the world (Lisbon Strategy). The scenarios explore in an integrated way the territorial impacts of future changes in the economy, transport, rural development, environment, and other policy fields. They do not only concentrate on the territorial impacts of the trends and driving forces but also of the relevant EU Policies. The scenarios throw a light on the effectiveness of EU Policies and the synergies as well as conflicts they generate. By doing this they provide important insights in the conditions under which these trends, driving forces and policies will favour a balanced and polycentric territory and territorial cohesion within the enlarging European Union. These insights are relevant for recommendations on possible adjustments and/or changes of EU Policies

Genome-wide fitness analyses of the foodborne pathogen Campylobacter jejuni in in vitro and in vivo models.

Campylobacter is the most common cause of foodborne bacterial illness worldwide. Faecal contamination of meat, especially chicken, during processing represents a key route of transmission to humans. There is a lack of insight into the mechanisms driving C. jejuni growth and survival within hosts and the environment. Here, we report a detailed analysis of C. jejuni fitness across models reflecting stages in its life cycle. Transposon (Tn) gene-inactivation libraries were generated in three C. jejuni strains and the impact on fitness during chicken colonisation, survival in houseflies and under nutrient-rich and -poor conditions at 4 °C and infection of human gut epithelial cells was assessed by Tn-insertion site sequencing (Tn-seq). A total of 331 homologous gene clusters were essential for fitness during in vitro growth in three C. jejuni strains, revealing that a large part of its genome is dedicated to growth. We report novel C. jejuni factors essential throughout its life cycle. Importantly, we identified genes that fulfil important roles across multiple conditions. Our comprehensive screens showed which flagella elements are essential for growth and which are vital to the interaction with host organisms. Future efforts should focus on how to exploit this knowledge to effectively control infections caused by C. jejuni.This work was funded by Biotechnology and Biological Sciences Research Council (http://www.bbsrc.ac.uk) grant BB/K004514/1. D.P.W. was funded by a Wellcome Trust (https://wellcome.ac.uk) Infection and Immunity PhD rotation studentship. The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript

The Bifidobacterium dentium Bd1 Genome Sequence Reflects Its Genetic Adaptation to the Human Oral Cavity

Bifidobacteria, one of the relatively dominant components of the human intestinal microbiota, are considered one of the key groups of beneficial intestinal bacteria (probiotic bacteria). However, in addition to health-promoting taxa, the genus Bifidobacterium also includes Bifidobacterium dentium, an opportunistic cariogenic pathogen. The genetic basis for the ability of B. dentium to survive in the oral cavity and contribute to caries development is not understood. The genome of B. dentium Bd1, a strain isolated from dental caries, was sequenced to completion to uncover a single circular 2,636,368 base pair chromosome with 2,143 predicted open reading frames. Annotation of the genome sequence revealed multiple ways in which B. dentium has adapted to the oral environment through specialized nutrient acquisition, defences against antimicrobials, and gene products that increase fitness and competitiveness within the oral niche. B. dentium Bd1 was shown to metabolize a wide variety of carbohydrates, consistent with genome-based predictions, while colonization and persistence factors implicated in tissue adhesion, acid tolerance, and the metabolism of human saliva-derived compounds were also identified. Global transcriptome analysis demonstrated that many of the genes encoding these predicted traits are highly expressed under relevant physiological conditions. This is the first report to identify, through various genomic approaches, specific genetic adaptations of a Bifidobacterium taxon, Bifidobacterium dentium Bd1, to a lifestyle as a cariogenic microorganism in the oral cavity. In silico analysis and comparative genomic hybridization experiments clearly reveal a high level of genome conservation among various B. dentium strains. The data indicate that the genome of this opportunistic cariogen has evolved through a very limited number of horizontal gene acquisition events, highlighting the narrow boundaries that separate commensals from opportunistic pathogens

The extent of increase in first calving age as a result of implementing various sexed semen breeding strategies

A deterministic simulation was conducted to assess the effects of sexed semen utilization strategies on age at first calving (AFC). Four different strategies were implemented on dairy heifers: continuous use of conventional semen only (CC), continuous use of sexed semen only (SS), utilization of sexed semen for both the first and second services with conventional semen afterwards (S2), and utilization of sexed semen for the first service with conventional semen afterwards (S1). Results indicated that continuous utilization of sexed semen led to the greatest AFC; however at high conception rates, strategies displayed negligible differences on AFC. Increases in estrus detection rate had the greatest effects on decreasing AFC of the SS scenarios. Negative effect of sexed semen on AFC increased when the effect of low estrus detection rate was combined with low conception rate of sexed semen. Results indicated that in the case of access to sexed semen conception rate, prediction of AFC is possible by quadratic polynomial or exponential equations, depending to the applied breeding strategy. Simultaneous utilization of sexed and conventional semen in a herd did not make a substantial change in AFC when a low percentage of sexed semen was employed. Increasing the contribution of different sexed semen strategies led to higher AFC variation, especially for the SS strategy. AFC of strategies that utilize sexed semen is highly dependent on the conception rate, estrus detection rate and the contribution of sex sorted semen in the total number of inseminations of the heifer her

Genetic requirements for Moraxella catarrhalis growth under iron-limiting conditions

Iron sequestration by the human host is a first line defence against respiratory pathogens like Moraxella catarrhalis, which consequently experiences a period of iron starvation during colonization. We determined the genetic requirements for M. catarrhalis BBH18 growth during iron starvation using the high-throughput genome-wide screening technology genomic array footprinting (GAF). By subjecting a large random transposon mutant library to growth under iron-limiting conditions, mutants of the MCR_0996-rhlB-yggW operon, rnd, and MCR_0457 were negatively selected. Growth experiments using directed mutants confirmed the GAF phenotypes with ΔyggW (putative haem-shuttling protein) and ΔMCR_0457 (hypothetical protein) most severely attenuated during iron starvation, phenotypes which were restored upon genetic complementation of the deleted genes. Deletion of yggW resulted in similar attenuated phenotypes in three additional strains. Transcriptional profiles of ΔyggW and ΔMCR_0457 were highly altered with 393 and 192 differentially expressed genes respectively. In all five mutants, expression of nitrate reductase genes was increased and of nitrite reductase decreased, suggesting an impaired aerobic respiration. Alteration of iron metabolism may affect nasopharyngeal colonization as adherence of all mutants to respiratory tract epithelial cells was attenuated. In conclusion, we elucidated the genetic requirements for M. catarrhalis growth during iron starvation and characterized the roles of the identified genes in bacterial growth and host interaction