Effect of the Photoexcitation Wavelength and Polarization on the Generated Heat by a Nd-Doped Microspinner at the Microscale

Thermal control at small scales is critical for studying temperature-dependent biological systems and microfluidic processes. Concerning this, optical trapping provides a contactless method to remotely study microsized heating sources. This work introduces a birefringent luminescent microparticle of NaLuF4:Nd3+ as a local heater in a liquid system. When optically trapped with a circularly polarized laser beam, the microparticle rotates and heating is induced through multiphonon relaxation of the Nd3+ ions. The temperature increment in the surrounding medium is investigated, reaching a maximum heating of ≈5 °C within a 30 μm radius around the static particle under 51 mW laser excitation at 790 nm. Surprisingly, this study reveals that the particle’s rotation minimally affects the temperature distribution, contrary to the intuitive expectation of liquid stirring. The influence of the microparticle rotation on the reduction of heating transfer is analyzed. Numerical simulations confirm that the thermal distribution remains consistent regardless of spinning. Instead, the orientation-dependence of the luminescence process emerges as a key factor responsible for the reduction in heating. The anisotropy in particle absorption and the lag between the orientation of the particle and the laser polarization angle contribute to this effect. Therefore, caution must be exercised when employing spinning polarization-dependent luminescent particles for microscale thermal analysis using rotation dynamics.Projects CNS2022-135495, PID2023-151078OB-I00 and TED2021-129937B-I00 funded by MCIN/AEI/10.13039/501100011033 and by the “European Union NextGenerationEU/PRTR”Spanish Ministerio de Universidades, through the FPU program (FPU19/04803)Consejería de Universidad, Investigación e Innovación de la Junta de Andalucía and by FEDER “Una manera de hacer Europa” (P18-FR-3583

Dislipidemias in Patients with Cardiopathy Isquemica

The dislipidemias are a risk factor well recognized of the cardiovascular diseases and constitute a problem of public health. A descriptive study in 150 patient elders of 30 years with diagnosis of Izquemic Cardiopathyes accomplished itself for the sake of identifying dislipidemias in patients of high cardiovascular risk that they helped the high-technology General Medical Center state James Mariño Aragua, at the Republic Bolivariana of Venezuela, that you constituted the sign of study from October 2011 to October 2012. They used quantitative and qualitative variables like weight, age, sex, pathological personal background, risk factors cardiovascular associates, seric levels of total cholesterol, triglycerides, HDL cholesterol, LDL cholesterol VLDL cholesterol. 63 percent of patients with dislipidemias were detected, being hypercholesterolemia the more alteration frequently found. The ages understood between 41 and 60 years evidenced the bigger frequency

REMOCIÓN DE CONTAMINANTES EN AGUAS RESIDUALES MEDIANTE HUMEDALES BIOINGENIERILES DOMICILIARIOS CON DIFERENTE TIPO DE SUSTRATO Y SEMBRADOS CON Strelitzia Reginae

El tratamiento de aguas residuales es una necesidad global y de gran interés, ya que la demanda de agua es cada vez mayor, además hay un volumen creciente de residuos biológicos y químicos que son arrojados directamente a los cuerpos de agua, lo que lleva a optar por una alternativa sustentable que remueva contaminantes del agua sin un control de operación complejo. El presente estudio tuvo como objetivo proponer y validar el uso de humedales construidos (HC) domiciliarios sembrados con ave de paraíso (Strelitzia reginae) para tratar aguas residuales. La etapa experimental se realizó en la localidad de Pastorías, Actopan (Veracruz) y consistió en 8 mesocosmos: 2 HC sembrados con Strelitzia reginae en piedra poroso de río como sustrato, 2 HC con la misma especie, pero sembrados en tezontle (piedra volcánica) y 2 HC solo con piedras, más 2 HC solo con tezontle, como controles. Las unidades operaron de manera continua durante 6 meses (marzo-agosto).                           La vegetación ornamental se adaptó a las condiciones de HC, en ambos sustratos el crecimiento fue similar. Los sustratos utilizados funcionaron similarmente en las remociones de los contaminantes, mejores resultados de remoción fueron observadas en presencia de la planta ornamental, en los mesocosmos con presencia de vegetación, tanto para sólidos totales (SST), amonio (N-NH4), fosfatos (P-PO4) y sulfatos (SO4) las remociones fueron hasta de 37%, 45%, 63% y 80%, respectivamente. Los HC con plantas ornamentales pueden realizar un tratamiento eficiente en las aguas residuales domésticas, Cabe mencionar que los sustratos son de bajo costo y fácil obtención. Palabras clave: Aguas residuals; Contaminantes; Humedales Construidos; Ornamentales; Remociones; Sustratos Locale

Light-to-Heat Conversion of Optically Trapped Hot Brownian Particles

Anisotropic hybrid nanostructures stand out as promising therapeutic agents in photothermal conversion-based treatments. Accordingly, understanding local heat generation mediated by light-to-heat conversion of absorbing multicomponent nanoparticles at the single-particle level has forthwith become a subject of broad and current interest. Nonetheless, evaluating reliable temperature profiles around a single trapped nanoparticle is challenging from all of the experimental, computational, and fundamental viewpoints. Committed to filling this gap, the heat generation of an anisotropic hybrid nanostructure is explored by means of two different experimental approaches from which the local temperature is measured in a direct or indirect way, all in the context of hot Brownian motion theory. The results were compared with analytical results supported by the numerical computation of the wavelength-dependent absorption efficiencies in the discrete dipole approximation for scattering calculations, which has been extended to inhomogeneous nanostructures. Overall, we provide a consistent and comprehensive view of the heat generation in optical traps of highly absorbing particles from the viewpoint of the hot Brownian motion theory.Grants PID2022-136919NA-C33, PID2019-106211RB-I00 (NANONERV), PID2021-127427NB-I00, PID2020-118448RBC21, PID2019-105195RA-I00, funded by the Ministerio de Ciencia e Innovación of Spain MCIN/AEI/10.13039/501100011033Grant CNS2022-135495, and TED2021-129937B-I00, funded by MCIN/AEI/10.13039/501100011033“European Union NextGenerationEU/PRTR” from the Ministerio de Economía, Industria y Competitividad of Spain (grant CTQ2017-86655-R) and from FEDER/Consejería de Transformación Económica, Industria, Conocimiento y Universidades of Andalucía (grants P18-FR-3583 and P20_00727/PAIDI2020)HORIZON-MSCA-2021-PF-01 grant agreement ID: 101065163Spanish Ministerio de Universidades, through the FPU program (FPU19/04803)Consejería de Salud y Familias (Junta de Andalucía) for his senior postdoctoral grant (RH-0040-2021

First findings and molecular data of phlebotomus mascittii (diptera: Psychodidae) in the cantabrian cornice (northern spain)

Phlebotomus (Transphlebotomus) mascittii Grassi, 1908 (Diptera: Psychodidae) has been found in several European countries. In Spain, sporadic records were reported in the early ''80s in Catalonia (Northeast Spain), and it was never detected again. Recent entomological surveys carried out between 2004 and 2020 revealed the presence of several specimens of P. mascittii in Spain. The species identification was confirmed by both morphological and molecular analyses. The analyzed specimens belonged to the haplotype (COI_2) defined by one polymorphic site compared to other European specimens. Phlebotomus mascittii was found in low population densities in rural areas associated with livestock farms and in an urban cemetery during the summer season. This study provides the first records of this species in various localities along the Cantabrian cornice (Northern Spain) and represents its westernmost observation in the Palearctic region. The implications of the finding of this uncommon species are discussed at different levels, with emphasis on its suspected role in the transmission of leishmaniosis. © The Author(s) 2021

Association of STAT4 with rheumatoid arthritis:A replication study in three European populations

OBJECTIVE: This study was undertaken to investigate the previously reported association of the STAT4 polymorphism rs7574865 with rheumatoid arthritis (RA) in 3 different European populations from Spain, Sweden, and The Netherlands, comprising a total of 2,072 patients and 2,474 controls. METHODS: Three different cohorts were included in the study: 923 RA patients and 1,296 healthy controls from Spain, 273 RA patients and 285 healthy controls from Sweden, and 876 RA patients and 893 healthy controls from The Netherlands. DNA from patients and controls was obtained from peripheral blood. Samples were genotyped for the STAT4 single-nucleotide polymorphism rs7574865 using a TaqMan 5'-allele discrimination assay. The chi-square test was performed to compare allele and genotype distributions. Odds ratios (ORs) and 95% confidence intervals (95% CIs) were calculated. RESULTS: We observed a significantly increased frequency of the minor T allele in RA patients compared with healthy controls in the Spanish population (24.8% versus 20.8%; P = 0.001, OR 1.26 [95% CI 1.09-1.45]). This association was confirmed in both the Swedish population (P = 0.03, OR 1.35 [95% CI 1.03-1.77]) and the Dutch population (P = 0.03, OR 1.45 [95% CI 1.21-1.73]). The overall P value for all 3 populations was 9.79 x 10(-6) (OR 1.25 [95% CI 1.13-1.37]). No association between rs7574865 and the presence of rheumatoid factor or anti-cyclic citrullinated peptide autoantibodies was observed. A meta-analysis of all published STAT4 associations revealed an OR of 1.25 (95% CI 1.19-1.33) (P = 1 x 10(-5)). CONCLUSION: Our findings indicate an association between the STAT4 polymorphism rs7574865 and RA in 3 different populations, from Spain, Sweden, and The Netherlands, thereby confirming previous data

Inventario preliminar y uso de mamíferos silvestres no voladores en la vereda Camarón, Montes de María (Bolívar-Colombia)

During the months of July and August 2015 and March 2016, field visits to the Camarón village were carried out, as part of the project “Towards the Knowledge and Study of the Wild Fauna in the Vereda Camarón and the Municipality of Santo Domingo de Meza, Montes de María (Bolívar). ”Direct traces were carried out in 5 linear transects of 1 km, two repetitions in each one, in order to obtain a preliminary list of mammal species in the area. Likewise, 140 semi-structured interviews were conducted with the community to determine the uses that communities give to these species. Interviews were preferably conducted with hunters and mammalian field guides were used (Emmons & Feer 1999, Aranda 2012) as well as cards with drawings and photographs of mammals present in the Colombian Caribbean (RaceroCasarrubia & González-Maya 2014).Durante los meses de julio y agosto de 2015 y marzo de 2016, se realizaron visitas de campo a la vereda Camarón, como parte del proyecto “Hacia el Conocimiento y Estudio de la Fauna Silvestre en la Vereda Camarón y el Municipio Santo Domingo de Meza, Montes de María (Bolívar).” Se realizaron rastreos directos en 5 transectos lineales de 1 km, dos repeticiones en cada uno, con el fin de obtener una lista preliminar de especies de mamíferos de la zona. Asimismo, se realizaron 140 entrevistas semiestructuradas a la comunidad para determinar los usos que las comunidades dan a estas especies. Las entrevistas se realizaron preferiblemente a cazadores y se utilizaron guías de campo de mamíferos (Emmons & Feer 1999, Aranda 2012) así como fichas con dibujos y fotografías de los mamíferos presentes en el Caribe colombiano (RaceroCasarrubia & González-Maya 2014).&nbsp

Combined Area of Left and Right Atria May Outperform Atrial Volumes as a Predictor of Recurrences after Ablation in Patients with Persistent Atrial Fibrillation—A Pilot Study

Background and Objectives: Left atrial (LA) remodelling and dilatation predicts atrial fibrillation (AF) recurrences after catheter ablation. However, whether right atrial (RA) remodelling and dilatation predicts AF recurrences after ablation has not been fully evaluated. Materials and Methods: This is an observational study of 85 consecutive patients (aged 57 ± 9 years; 70 [82%] men) who underwent cardiac magnetic resonance before first catheter ablation for AF (40 [47.1%] persistent AF). Four-chamber cine-sequence was selected to measure LA and RA area, and ventricular end-systolic image phase to obtain atrial 3D volumes. The effect of different variables on event-free survival was investigated using the Cox proportional hazards model. Results: In patients with persistent AF, combined LA and RA area indexed to body surface area (AILA + RA) predicted AF recurrences (HR = 1.08, 95% CI 1.00-1.17, p = 0.048). An AILA + RA cut-off value of 26.7 cm2/m2 had 72% sensitivity and 73% specificity for predicting recurrences in patients with persistent AF. In this group, 65% of patients with AILA + RA > 26.7 cm2/m2 experienced AF recurrence within 2 years of follow-up (median follow-up 11 months), compared to 25% of patients with AILA + RA ≤ 26.7 cm2/m2 (HR 4.28, 95% CI 1.50-12.22; p = 0.007). Indices of LA and RA dilatation did not predict AF recurrences in patients with paroxysmal AF. Atrial 3D volumes did not predict AF recurrences after ablation. Conclusions: In this pilot study, the simple measurement of AILA + RA may predict recurrences after ablation of persistent AF, and may outperform measurements of atrial volumes. In paroxysmal AF, atrial dilatation did not predict recurrences. Further studies on the role of RA and LA remodelling are needed

Genome-wide meta-analysis reveals shared new loci in systemic seropositive rheumatic diseases

Objective: Immune-mediated inflammatory diseases (IMIDs) are heterogeneous and complex conditions with overlapping clinical symptoms and elevated familial aggregation, which suggests the existence of a shared genetic component. In order to identify this genetic background in a systematic fashion, we performed the first cross-disease genome-wide meta-analysis in systemic seropositive rheumatic diseases, namely, systemic sclerosis, systemic lupus erythematosus, rheumatoid arthritis and idiopathic inflammatory myopathies. Methods: We meta-analysed ~6.5million single nucleotide polymorphisms in 11 678 cases and 19 704 non-affected controls of European descent populations. The functional roles of the associated variants were interrogated using publicly available databases. Results: Our analysis revealed five shared genome-wide significant independent loci that had not been previously associated with these diseases: NAB1, KPNA4-ARL14, DGQK, LIMK1 and PRR12. All of these loci are related with immune processes such as interferon and epidermal growth factor signalling, response to methotrexate, cytoskeleton dynamics and coagulation cascade. Remarkably, several of the associated loci are known key players in autoimmunity, which supports the validity of our results. All the associated variants showed significant functional enrichment in DNase hypersensitivity sites, chromatin states and histone marks in relevant immune cells, including shared expression quantitative trait loci. Additionally, our results were significantly enriched in drugs that are being tested for the treatment of the diseases under study. Conclusions: We have identified shared new risk loci with functional value across diseases and pinpoint new potential candidate loci that could be further investigated. Our results highlight the potential of drug repositioning among related systemic seropositive rheumatic IMIDs