Hepatic Cytochromes P450 Enzyme Activities of UAE Coastal Rabbit Safi Fish Siganus canaliculatus

The cytochrome P450 dependent metabolism of benzo(a)pyrene and other xenobiotics has been investigated in liver microsomes prepared from a local marine Safi fish Siganus canaliculatus caught from the Arabian Gulf of United Arab Emirates. The Safi fish was found to have a well developed liver microsomal monooxygenase system consisting of cytochrome P450, cytochrome b5 and NADPH-cytochrome c reductase. The fish microsomal enzyme system was able to metabolise benzo(a)pyrene, 7-ethoxycoumarin and 7- ethoxyresorufin. Male fish were found to exhibit a higher monooxygenase activity than female fish. Treatment of fish with β-naphthoflavone was found to induce (2-4 fold) the activities of aryl hydrocarbon hydroxylase, ethoxycoumarin O-deethylase and ethoxyresorufin O-deethylase. In vitro addition of α-naphthoflavone, a specific inhibitor of β-naphthoflavone inducible cytochrome P450s activities was found to inhibit the enzyme activities. These findings suggest the presence of various forms of cytochrome P450 in Safi fish liver. Western immunoblot analysis of liver microsomes from β-naphthoflavone treated fish using an antibody to rat liver cytochrome P450 1A1 (CYP1A1) suggested the presence of an inducible cytochrome P450 enzyme which was homologous to the mammalian CYP1A1 and was comparable with that of rat liver enzyme. Our results suggest that liver microsomes from the Safi fish have multiple forms of cytochrome P450 with a specific β-naphtho-flavone inducible CYP1A 1 homologous protein that can metabolise a variety of carcinogenic and non-carcinogenic substrates. Our results also indicate that like rats and mice and some other marine fish, Safi fish could also be used as a sensitive tool for the monitoring of the Arabian Gulf region pollution, especially with polycyclic hydrocarbons of the coastal water

Early Literacy Instruction and Intervention

The purpose of this paper is to describe the efficacy of early literacy interventions and to discuss possible roles for volunteer tutors in helping prevent reading difficulties within the Response to Intervention process. First, we describe a landmark study that evaluated the impact of primary classroom instruction on reducing the proportion of students at risk for reading failure, and a more recent series of studies exploring the effects of individualizing classroom reading instruction based on students’ initial skills. Second, we review studies of more intensive early intervention to demonstrate how these interventions substantially reduce the proportion of students at risk. Third, we examine effective tutoring models that utilize volunteers. Finally, we discuss the potential role of community tutors in supporting primary classroom instruction and secondary interventions

RESPONSE TO INTERVENTION

The purpose of this interdisciplinary paper is to describe Response to Intervention (RTI), or multi-tier systems of support, for early literacy to improve reading outcomes for students with or at risk for reading disability. First we review the current US policy on RTI for the purpose of early literacy intervention and for identification of reading disabilities. We situate this within recent efforts in developing countries supported by the World Bank and the Early Grade Reading Assessments. Then, we highlight a large experimental study we conducted with first graders and provide findings from a third grade follow up. We conclude with implications for research, practice, and policy

RESPONSE TO INTERVENTION

The purpose of this interdisciplinary paper is to describe Response to Intervention (RTI), or multi-tier systems of support, for early literacy to improve reading outcomes for students with or at risk for reading disability. First we review the current US policy on RTI for the purpose of early literacy intervention and for identification of reading disabilities. We situate this within recent efforts in developing countries supported by the World Bank and the Early Grade Reading Assessments. Then, we highlight a large experimental study we conducted with first graders and provide findings from a third grade follow up. We conclude with implications for research, practice, and policy

Evidence-Based Reading Instruction for Individuals With Autism Spectrum Disorders

Legislation mandates that all children, including children with autism spectrum disorders (ASD), be taught to read in ways that are consistent with reading research and target the five components of evidence-based reading instruction: phonemic awareness, phonics, reading fluency, vocabulary, and comprehension strategies. This review synthesized the literature on reading instruction for children with ASD that encompassed one or more of the five components of reading. The review included 11 studies with 61 participants ages 4 to 17 years. Results indicated that children with ASD can benefit from reading instruction consistent with reading research. Research in this area is still preliminary, and more research is needed to guide practice. Possible directions for future research are provided

Socioeconomic and gender group differences in early literacy skills: a multiple-group confirmatory factor analysis approach

Socioeconomic status and gender are important demographic variables that strongly relate to academic achievement. This study examined the early literacy skills differences between 4 sociodemographic groups, namely, boys ineligible for free or reduced-price lunch (FRL), girls ineligible for FRL, boys eligible for FRL, and girls eligible for FRL. Data on kindergarteners (N = 462) were analysed using multiplegroup confirmatory factory analysis. Early literacy skill differences between boys and girls are more nuanced than previously reported; subsidy status and gender interact. Both boys and girls from high-poverty households performed significantly lower than the girls from low-poverty households in alphabet knowledge, phonological awareness, and spelling. There were gender gaps, with a female advantage, among children from high-poverty households in alphabet knowledge and spelling and among children from low-poverty households in alphabet knowledge. These results highlight the importance of employing methodologically sound techniques to ascertain group differences in componential early literacy skills

Student Behavior Ratings and Response to Tier 1 Reading Intervention: Which Students Do Not Benefit?

Core reading instruction and interventions have differential effects based on student characteristics such as cognitive ability and pre-intervention skill level. Evidence for differential effect based on affective characteristics is scant and ambiguous; however, students with problem behavior are more often non-responsive to core reading instruction and intensive reading interventions. In this study, we estimated the range of students\u27 behavior ratings in which a core reading instruction intervention was effective using a data set including 3,024 students in K-3. Data came from seven independent studies evaluating the individualized Student Instruction (ISI) Tier 1 reading intervention and were pooled using integrative data analysis. We estimated Johnson-Neyman intervals of student behavior ratings that showed a treatment effect both at the within and between classroom level. ISI was effective in improving reading scores (b = 0.51, p = .020, d = 0.08). However, students with very low or very high behavior ratings did not benefit from the approaches (range of behavior rating factor scores: -0.95-2.87). At the classroom level, students in classrooms with a higher average of problem behaviors did not benefit from ISI (average classroom behavior rating factor score: 0.05-4.25). Results suggest differentiating instruction alone is not enough for students with behavior problems to grow in reading ability

Home Literacy Environments of Young Children With Down Syndrome Findings From a Web-Based Survey

Early home literacy experiences, including parent-child book reading, account for a significant amount of children\u27s later reading achievement. Yet there is a very limited research base about the home literacy environments and experiences of children with cognitive disabilities. The purpose of this study is to describe findings from a Web-based survey of home literacy environments of young children with Down syndrome. Respondents (n = 107) were mostly mothers; a majority were well educated. Findings suggest that respondents gave literacy a higher priority than reported in prior research on children with disabilities. More than 70% of respondents had 50 or more children\u27s books and also had literacy materials including flash cards, magnetic letters, and educational videos or computer games. Most parents read to their children and used these literacy materials 10 to 30 min per day. Respondents reported that their children had reached many important early literacy milestones, and they also described having relatively ambitious lifelong literacy goals for their children. Important implications for research and practice are discussed

Direct impact of mindset on reading-based outcomes in upper elementary students with reading difficulties

Mindset involves an array of beliefs related to the malleability of certain attributes, including intelligence. One’s growth mindset comprises a variety of cognitive and motivational factors (e.g., learning goals, effort beliefs) and the perception that skill attainment and outcome in any domain are tethered to personal effort. A fixed mindset encompasses goals, beliefs, and perceptions that skill level is not malleable or impacted by effort. Structural equation models tested investigated direct and indirect pathways of mindset and motivational variables (i.e., learning goals, effort beliefs) with reading outcomes (i.e., word reading, fluency, reading comprehension) in a diverse sample of fourth grade students (n = 408) with reading difficulties. Results revealed a direct impact of fixed mindset (−0.21 to −0.36) and effort beliefs (0.18–0.31) on all standardized, reading-based outcomes. There were no significant mediating pathways. Results are juxtaposed to other research in this area, and practical implications are discussed. Growth mindset appears a robust predictor of positive academic outcomes, and it may be beneficial to facilitate growth mindset in classrooms