Effect of Cytotoxicity of Pegylated Liposomal Recombinant Human Erythropoietin-alfa on Neuroblastoma Cell Line SHSY5Y

Purpose: To evaluate the cytotoxic effect of pegylated liposomal Recombinant Human Erythropoietinalfa (rHuEPO) nanoparticles synthesized by reverse phase evaporation technique on SH-SY5Y cell line.Methods: To prepare the nanoparticles of the drug, rHuEPO, PEG3000, cholesterol andphosphatidylcholine were dissolved in buffer phosphate. The characteristics of the synthesized nanoparticles were determined by a zetasizer. Encapsulation efficiency, drug loading efficiency and drug release pattern were evaluated spectrophotometrically. The cytotoxicity effect of pegylated nanoliposomal rHuEPO was evaluated on SH-SY5Y cell line by 3-(4,5-dimethylthiazol-2-yl)-2,5- diphenyltetrazolium bromide (MTT) assay.Results: Size and zeta potential of pegylated nanoliposomal drug and blank pegylated nanoliposomes were 571 ± 6.8 nm, -16.5 mV; 280 ± 4.71 nm and -27.1 mV, respectively. Encapsulation and drug loading efficiency were 89 ± 3.9 % and 0.8 ± 0.012 %, respectively. Drug release data indicate that 17 % of rHuEPO was released from pegylated liposomal nanoparticles over a period of 30 h. The difference in cytotoxicity between the free drug (IC50 = 110.1 ± 3.1 μg/ml) and nanodrug (IC50 = 87.2 ± 2.3 μg/ml) was statistically significant (p ˂ 0.05).Conclusion: This study shows that pegylated nanoliposomal rHuEPO has a powerful cytotoxic effect on SH-SY5Y cell line and is therefore a suitable alternative to the standard therapy, rHuEPO, for the chemotherapy of neuroblastoma.Keywords: Recombinant Human Erythropoietin-alfa, Cytotoxicity, Drug delivery, Liposome, Reverse phase evaporation, SH-SY5Y cell lin

The Effect of Stevia Rebaudiana on Serum Omentin and Visfatin Level in STZ-Induced Diabetic Rats

Recently the role of adipocytokines in relationship to incidence of diabetes has been demonstrated. One of the medicinal plants that are used in the treatment of diabetes is stevia. This study investigates the effect of stevia on serum omentin and visfatin levels as novel adipocytokines in diabetic induced rats to find potential mechanisms for the anti hyperglycemic effect of stevia. Forty male wistar rats weighing 180–250 g were induced with diabetes by intraperitoneal injection of streptozotocin (STZ). The animals were divided into 5 groups of 8. Rats in group 1 (non-diabetic control) and group 2 (diabetic control) were treated with distilled water, and the rats in the treated groups, group 3 (T250), group 4 (T500), and group 5 (T750) were treated with stevia, gavaged every day at 9 a.m. in doses of 250, 500, and 750 mg/kg, respectively. At the end of the study significant reductions in fasting blood sugar (FBS), the homeostasis model assessment insulin resistance (HOMA-IR), triglyceride (TG), alkaline phosphatase (ALP), and Omentin level were found in groups 3 and 4 in comparison with group 2. Pancreatic histopathology slides demonstrated that stevia extract did not induce any increase in the number of β-cells. The conclusion is that prescription of stevia in the doses of 250 and 500 mg/kg/d decreases the omentin level indirectly via activating insulin sensitivity and lowering blood glucose in STZ-induced diabetic rats. KEYWORDS. Diabetes, omentin, stevia, visfati

Species identification of Middle Eastern blowflies (Diptera: Calliphoridae) of forensic importance.

The lack of reliable tools for species identification of necrophagous blowflies of the Middle East is a serious obstacle to the development of forensic entomology in the majority of countries of this region. Adding to the complexity of diagnosing the regional fauna is that species representing three different zoogeographical elements exist in sympatry. In response to this situation, a high-quality key to the adults of all species of forensically relevant blowflies of the Middle East has been prepared. Thanks to the modern technique of image-stack stereomicroscopy and high-quality entomological materials, this new key can be easily applied by investigators inexperienced in the taxonomy of blowflies. The major technical problems relating to the species identification of necrophagous blowflies of the Middle East are also discussed

Dragonfly‐inspired wing design enabled by machine learning and Maxwell's reciprocal diagrams

Number theory, Algebra and Geometr

Effect of a nanocomposite containing ostrich eggshell on calvarium healing in the rabbit: a pathologic study

ΔΕΝ ΔΙΑΤΙΘΕΤΑΙ ΠΕΡΙΛΗΨΗThe aim of the current study was to determine effect of a nanocomposite containing ostrich eggshell (NCOE) on the calvarium healing in the rabbit. Fresh ostrich eggshell was ground (300-500 μm), treated in phosphate-containing solutions and sterilized by gamma irradiation. Fifteen New Zealand white adult male rabbits were used. Four full-thickness skull defects were created in the calvarium. The first defect kept unfilled (control). The second defect was filled with autograft bone. The third defect was filled using NCOE. The fourth defect was filled with mixture of the autograft+NCOE bone. At 30, 60 and 90 days after surgery animals were euthanized and tissue specimens were collected and stained with hematoxylin eosin and trichrome staining method. Microsections were examined toassess the extent and intensity of inflammation, calvarium formation status and foreign body reaction. According to the results, filling defect significantly increased in NCOE-treated rabbits compared to the control group at 30 and 60 days post-surgery (P<0.05). There a statistically significant difference between experimental groups compared to the control group at 30 and 60 days post-surgery (P<0.05) while no statistically significant differences were observed among autograft, NCOE, autograft+NCOE (P>0.05). Also, absorb material significantly decreased in NCOE and autograft+NCOE groups compared to the control group at 60 days post-surgery (P<0.05). The filling defect significantly increased in autograft, NCOE and NCOE+autograft groups compared to the control group at 90 days post-surgery (P<0.05). There was no significant difference on inflammation and absorb material among the groups at 90 days post-surgery (P>0.05). These results suggested NCOE+autograft has improved the rate of calvarium healing in rabbits

Effects of water temperature on filtration rate of micro-algae Isochrysis affines galbana by black lip pearl oyster spat (Pinctada margaritifera)

This study was conducted to compare the effects of varying water temperatures on filtration rate of micro-algae Isochrysis affines galbana by Pinctada margaritfera spat on microalgae. To determine the optimum temperature and maximum filtration rate, 10 pearl oyster. margaritfera spats with mean total and total hinge length 32.67 ±2.76 and 31.57 ±2.90 respectively were stocked in 70 liter rectangular glass tanks supplied with filtered seawater. The spats were exposed to seven treatments of temperature including 18.5 ±0.21°C, 20.5 ±0.21°C, 23 ±0.1°C, 24.5 ±0.31°C, 26.5 ±0.21°C, 27.5 ±0.15°C and 28.5 ±0.21°C with 3 replicates. The results of filtration on I. affines galbana showed that the maximum filtration of spats on microalgae cells and water volume were 181,324,595 cells and 3626 ml per hour and 635,031,212 cells and 12700 ml per two hours in 27.5 ±0.15°C, respectively. The minimum filtration of microalgae cells and water volume were 5,224,028 and 104ml per hour and 65,874,413 cells and 1317 ml per two hours in 18.50 ±021C, respectively. The results of this study demonstrated that the optimum temperature for maximum feeding of spats on I. affines galbana is 27.5 ±0.15°C

Density functional approach to study structural properties and Electric Field Gradients in rare earth materials

We investigated the effect of spin polarization on the structural properties and gradient of electric field (EFG) on Sn, In, and Cd impurity in RSn$_3$ (R=Sm, Eu, Gd) and RIn$_3$ (R=Tm, Yb, Lu) compounds. The calculations were performed self-consistently using the scalar-relativistic full potential linearized augmented plane wave method. The local density approximations (LDA) and generalized gradient approximation without spin polarization (GGA) and with spin polarization (GGA+SP) to density functional theory were applied. In addition to that we performed some calculations within open core treatment (GGA+open core). It is clearly seen that GGA+SP is successful in predicting the larger lattice parameter and the dramatic drop of EFG for R=(Eu, Yb) relative to other rare earth compounds. This is an indication that spin splitting generated by spin polarization without any modification, is capable of treating properly the highly correlated f electrons in these systems.Comment: 14 Pages, 3 Figures, 5 Table

Modeling and performance evaluation of the eICIC/ABS in H-CRAN

International audienceIn this paper, we propose mathematical models to evaluate the performance of the interference remediation technique eICIC/ABS (enhanced Inter-Cell Interference Coordination / Almost Blank Sub-frame) in the context of Heterogeneous Cloud based Radio Access Networks (H-CRAN) architecture and 5G networks. The objective is to propose a dynamic resource management tool to ease decisions on the activation/deactivation of micro-cells as well as on the distributions of subframes among macro and micro cells. First, we propose a Markov chain based model that fits the behavior of the considered scheme and allows the analysis of the cell throughput according to traffic load, radio conditions and the distribution of available resources among macro and micro cells. Then, we propose an approximation model with a closed form formula. The two models are validated and evaluated in terms of accuracy and computation time. Numerical results are compared to matlab simulations that reproduce realistic radio conditions. Results show that both models are accurate. However, the closed form approximation is less complex and provides faster results