Administración de cuentas por pagar de la empresa Fabrirex SAC, Ancon-2014

La investigación que se ha titulado “LA ADMINISTRACION DE CUENTAS POR PAGAR DE LA EMPRESA FABRIREX SAC, ANCON-2014”, ha dado respuesta al problema ¿Cuál es el nivel de la administración de cuentas por pagar de la empresa FABRIREX SAC., Ancon-2014?, teniendo como objetivo general Determinar el nivel de la Administración de Cuentas por Pagar de la empresa FABRIREX SAC., Ancon-2014. La metodología empleada para la elaboración de la tesis, estuvo relacionada al método deductivo-inductivo. El diseño de la investigación ha sido no experimental. La muestra estuvo representada por 25 trabajadores de la empresa FABRIREX SAC. .La técnicas de investigación utilizada fue la encuesta para recoger la información y el instrumento el cuestionario. Entre los resultados más importantes obtenidos con la prueba estadística de Alfa de cronbach tenemos lo siguiente, el valor de 0.831; lo cual indica que tiene una tendencia al nivel medianamente eficiente

Alanine Represses γ-Aminobutyric Acid Utilization and Induces Alanine Transaminase Required for Mitochondrial Function in Saccharomyces cerevisiae

The γ-aminobutyric acid (GABA) shunt constitutes a conserved metabolic route generating nicotinamide adenine dinucleotide phosphate (NADPH) and regulating stress response in most organisms. Here we show that in the presence of GABA, Saccharomyces cerevisiae produces glutamate and alanine through the irreversible action of Uga1 transaminase. Alanine induces expression of alanine transaminase (ALT1) gene. In an alt1Δ mutant grown on GABA, alanine accumulation leads to repression of the GAD1, UGA1, and UGA2 genes, involved in the GABA shunt, which could result in growth impairment. Induced ALT1 expression and negative modulation of the GABA shunt by alanine constitute a novel regulatory circuit controlling both alanine biosynthesis and catabolism. Consistent with this, the GABA shunt and the production of NADPH are repressed in a wild-type strain grown in alanine, as compared to those detected in the wild-type strain grown on GABA. We also show that heat shock induces alanine biosynthesis and ALT1, UGA1, UGA2, and GAD1 gene expression, whereas an uga1Δ mutant shows heat sensitivity and reduced NADPH pools, as compared with those observed in the wild-type strain. Additionally, an alt1Δ mutant shows an unexpected alanine-independent phenotype, displaying null expression of mitochondrial COX2, COX3, and ATP6 genes and a notable decrease in mitochondrial/nuclear DNA ratio, as compared to a wild-type strain, which results in a petite phenotype. Our results uncover a new negative role of alanine in stress defense, repressing the transcription of the GABA shunt genes, and support a novel Alt1 moonlighting function related to the maintenance of mitochondrial DNA integrity and mitochondrial gene expression.Peer Reviewe

High-precision U-Pb ages in the early Tithonian to early Berriasian and implications for the numerical age of the Jurassic-Cretaceous boundary

The numerical age of the Jurassic-Cretaceous boundary has been controversial and difficult to determine. In this study, we present high-precision U-Pb geochronological data around the Jurassic-Cretaceous boundary in two distinct sections from different sedimentary basins: the Las Loicas, Neuquén Basin, Argentina, and the Mazatepec, Sierra Madre Oriental, Mexico. These two sections contain primary and secondary fossiliferous markers for the boundary as well as interbedded volcanic ash horizons, allowing researchers to obtain new radioisotopic dates in the late Tithonian and early Berriasian. We also present the first age determinations in the early Tithonian and tentatively propose a minimum duration for the stage as a cross-check for our ages in the early Berriasian. Given our radioisotopic ages in the early Tithonian to early Berriasian, we discuss implications for the numerical age of the boundary.Fil: Lena, Luis. Universidad de Ginebra; SuizaFil: López Martínez, Rafael. Universidad Nacional Autónoma de México; MéxicoFil: Lescano, Marina Aurora. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Ciudad Universitaria. Instituto de Estudios Andinos "Don Pablo Groeber". Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Instituto de Estudios Andinos "Don Pablo Groeber"; ArgentinaFil: Aguirre-Urreta, Maria Beatriz. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Ciudad Universitaria. Instituto de Estudios Andinos "Don Pablo Groeber". Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Instituto de Estudios Andinos "Don Pablo Groeber"; ArgentinaFil: Concheyro, Graciela. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Ciudad Universitaria. Instituto de Estudios Andinos "Don Pablo Groeber". Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Instituto de Estudios Andinos "Don Pablo Groeber"; ArgentinaFil: Vennari, Verónica Vanesa. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Ciudad Universitaria. Instituto de Estudios Andinos "Don Pablo Groeber". Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Instituto de Estudios Andinos "Don Pablo Groeber"; ArgentinaFil: Naipauer, Maximiliano. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Ciudad Universitaria. Instituto de Estudios Andinos "Don Pablo Groeber". Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Instituto de Estudios Andinos "Don Pablo Groeber"; ArgentinaFil: Samankassou, Elias. Universidad de Ginebra; SuizaFil: Pimentel, Márcio. Universidade do Brasília; BrasilFil: Ramos, Victor Alberto. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Ciudad Universitaria. Instituto de Estudios Andinos "Don Pablo Groeber". Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Instituto de Estudios Andinos "Don Pablo Groeber"; ArgentinaFil: Schaltegger, Urs. Universidad de Ginebra; Suiz

Vinyl sulfone silica: application of an open preactivated support to the study of transnitrosylation of plant proteins by S-nitrosoglutathione

Background S-nitrosylaton is implicated in the regulation of numerous signaling pathways with a diversity of regulatory roles. The high lability of the S-NO bond makes the study of proteins regulated by S-nitrosylation/denitrosylation a challenging task and most studies have focused on already S-nitrosylated proteins. We hypothesize that: i) S-nitrosoglutathione (GSNO) transnitrosylation is a feasible mechanism to account for the physiological S-nitrosylation of rather electropositive sulfur atoms from proteins, ii) affinity chromatography is a suitable approach to isolate proteins that are prone to undergo S-transnitrosylation and iii) vinyl sulfone silica is a suitable chromatographic bead.Results The combination of vinyl sulfone silica with GSNO yielded an affinity resin that withstood high ionic strength without shrinking or deforming and that it was suitable to isolate potential GSNO transnitrosylation target candidates. Fractions eluted at 1500 mM NaCl resulted in a symmetrical peak for both, protein and S-nitrosothiols, supporting the idea of transnitrosylation by GSNO as a selective process that involves strong and specific interactions with the target protein. Proteomic analysis led to the identification of 22 physiological significant enzymes that differ with the tissue analyzed, being regulatory proteins the most abundant group in hypocotyls. The identification of chloroplastidic FBPase, proteasome, GTP-binding protein, heat shock Hsp70, syntaxin, catalase I, thioredoxin peroxidase and cytochrome P450 that have already been reported as S-nitrosylated by other techniques can be considered as internal positive controls that validate our experimental approach. An additional validation was provided by the prediction of the S-nitrosylation sites in 19 of the GSNO transnitrosylation target candidates.Conclusions Vinyl sulfone silica is an open immobilization support that can be turned ad hoc and in a straightforward manner into an affinity resin. Its potential in omic sciences was successfully put to test in the context of the analysis of post-translational modification by S-nitrosylation with two different tissues: mature pea leaves and embryogenic sunflower hypocotyls. The identified proteins reveal an intriguing overlap among S-nitrosylation and both tyrosine nitration and thioredoxin regulation. Chloroplastidic FBPase is a paradigm of such overlap of post-translational modifications since it is reversible modified by thioredoxin and S-nitrosylation and irreversibly by tyrosine nitration. Our results suggest a complex interrelation among different modulation mechanisms mediated by NO-derived molecules.Financial Support was provided by Dirección General de Investigacion Cientıfica y Técnica (DGICYT) (CTQ2008-01754), Junta de Andalucía (P07-FQM-02899), Universidad de Jaén campus de Excelencia Internacional Agroalimentario ceiA3 and by ERDF-cofinanced grants from Ministry of Science and Innovation (BIO2012-33904) and Junta de Andalucía (research groups BIO286 and BIO192). We also acknowledge support of the publication fee by the CSIC Open Access Publication Support Initiative through its Unit of Information Resources for Research (URICI)

Dual regulation of cytosolic ascorbate peroxidase (APX) by tyrosine nitration and S-nitrosylation

JBM acknowledges a PhD fellowship (F.P.U.) from the Ministry of Science and Innovation. This work was supported by an ERDF-co-financed grant from the Ministry of Science and Innovation (BIO2009-12003-C02-01, BIO2009-12003-C02-02, and BIO2012-33904) and Junta de Andalucia (group BIO286 and BIO192), Spain. LC/MS/MS analyses were carried out at the Laboratorio de Proteomica LP-CSIC/UAB, a member of the ProteoRed network. Technical and human support provided by CICT of Universidad de Jaen (UJA, MINECO, Junta de Andalucia, FEDER) is gratefully acknowledged. We acknowledge Mr Carmelo Ruiz-Torres for his excellent technical support.Post-translational modifications (PTMs) mediated by nitric oxide (NO)-derived molecules have become a new area of research, as they can modulate the function of target proteins. Proteomic data have shown that ascorbate peroxidase (APX) is one of the potential targets of PTMs mediated by NO-derived molecules. Using recombinant pea cytosolic APX, the impact of peroxynitrite (ONOO–) and S-nitrosoglutathione (GSNO), which are known to mediate protein nitration and S-nitrosylation processes, respectively, was analysed. While peroxynitrite inhibits APX activity, GSNO enhances its enzymatic activity. Mass spectrometric analysis of the nitrated APX enabled the determination that Tyr5 and Tyr235 were exclusively nitrated to 3-nitrotyrosine by peroxynitrite. Residue Cys32 was identified by the biotin switch method as S-nitrosylated. The location of these residues on the structure of pea APX reveals that Tyr235 is found at the bottom of the pocket where the haem group is enclosed, whereas Cys32 is at the ascorbate binding site. Pea plants grown under saline (150mM NaCl) stress showed an enhancement of both APX activity and S-nitrosylated APX, as well as an increase of H2O2, NO, and S-nitrosothiol (SNO) content that can justify the induction of the APX activity. The results provide new insight into the molecular mechanism of the regulation of APX which can be both inactivated by irreversible nitration and activated by reversible S-nitrosylation.Spanish GovernmentERDF from the Ministry of Science and Innovation BIO2009-12003-C02-01 BIO2009-12003-C02-02 BIO2012-33904Junta de Andalucia BIO286 BIO192CICT of Universidad de Jaen (UJA, MINECO, Junta de Andalucia, FEDER

Identification of germline cancer predisposition variants in pediatric sarcoma patients from somatic tumor testing

Genetic predisposition is an important risk factor for cancer in children and adolescents but detailed associations of individual genetic mutations to childhood cancer are still under intense investigation. Among pediatric cancers, sarcomas can arise in the setting of cancer predisposition syndromes. The association of sarcomas with these syndromes is often missed, due to the rarity and heterogeneity of sarcomas and the limited search of cancer genetic syndromes. This study included 43 pediatric and young adult patients with different sarcoma subtypes. Tumor profiling was undertaken using the Oncomine Childhood Cancer Research Assay (Thermo Fisher Scientific). Sequencing results were reviewed for potential germline alterations in clinically relevant genes associated with cancer predisposition syndromes. Jongmans´ criteria were taken into consideration for the patient selection. Fifteen patients were selected as having potential pathogenic germline variants due to tumor sequencing that identified variants in the following genes: CDKN2A, NF1, NF2, RB1, SMARCA4, SMARCB1 and TP53. The variants found in NF1 and CDKN2A in two different patients were detected in the germline, confirming the diagnosis of a cancer predisposition syndrome. We have shown that the results of somatic testing can be used to identify those at risk of an underlying cancer predisposition syndrome.This work was funded by Research Projects from Navarra Government (Ref. 54/2018), the Jesús de Gangoiti Barrera Foundation (FJGB18/004 and FJGB19/001), Asociación Pablo Ugarte APU (APU-osteosarcoma), La Cuadri del Hospi (BC/A/17/008), EITB Media AND BIOEF, SAU (BIO20/CI/015/BCB and BIO20/CI/011/BCB), Basque Government (2021111030) and Fundación La Caixa with Niños Contra el Cáncer. P.A.-P. is supported by a Basque Government fellowship (PRE_2021_2_0048)

Comparación electromiográfica de músculos suprahioideos, masetero y orbicular de los labios en niños y niñas de 1 a 5 meses de edad, alimentados con lactancia materna o biberón

ABSTRACT: A non probabilistic sample of 20 children between 1 and 5 months of age was selected, 10 children were breast fed and 10 bottle fed to which electromyography with surface electrodes was performed on the supra hyoid muscles, masseter and orbicularis oris. For each muscle, 20 action potentials were selected, which meant a total of 200 action potentials per muscle in each group. It was found that the highest muscle activity in both groups was in the supra hyoids, followed by the orbicularis oris and the last the masseter. There were statically significant differences between the activity of the supra hyoids and orbicularis oris, but not with the masseter. The highest activity of the supra hyoid muscles was found in the breast fed group, and the highest activity of the orbicularis oris was in the bottle fed group.RESUMEN: Se tomó una muestra no probabilística de 20 niños de 1 a 5 meses de edad, 10 niños alimentados con lactancia materna y 10 niños alimentados con biberón, a los cuales se les realizó electromiografías con electrodos de superficie en los músculos suprahioideos, masetero y orbicular de los labios. Para cada músculo se seleccionaron 20 potenciales de acción, lo que implicó un total de 200 potenciales de acción por músculo en cada grupo. Se encontró que la mayor actividad muscular en ambos grupos fue en los suprahioideos, seguida por la del orbicular de los labios y por último la del masetero. Hubo diferencias estadísticamente significativas entre ambos grupos en la actividad de los suprahioideos y orbicular de los labios, mas no en el masetero. La mayor actividad de los músculos suprahioideos fue en el grupo de niños alimentados con lactancia materna y la mayor actividad del músculo orbicular de los labios fue en el grupo de niños alimentados con biberón

Contribución del anillamiento al conocimiento y conservación de las aves en España: pasado, presente y futuro

El anillamiento científico de aves es una técnica de estudio con más de un siglo de historia que, probablemente, ha contribuido como ninguna otra metodología al conocimiento de la biología de este grupo faunístico. A pesar del desarrollo de nuevas tecnologías, el marcaje individual de aves mediante anillamiento sigue siendo una técnica plenamente vigente y necesaria. Aunque la evidencia científica sobre los beneficios de la aplicación del anillamiento en la Ornitología moderna es abrumadora, hoy vivimos un proceso de creciente desinformación que cuestiona el anillamiento de aves y su utilidad. Este dosier se ha elaborado con el fin de ofrecer una visión actualizada de la utilidad del anillamiento científico de aves en España. Ha sido elaborado por un nutrido grupo de expertos asociados a universidades y centros de investigación que abarcan buena parte de las áreas del conocimiento implicadas en el estudio y conservación de las aves. El dosier se divide en cuatro grandes apartados. (1) En primer lugar se hace una introducción sobre el anillamiento como metodología y se resumen las grandes cifras del anillamiento en España donde, hasta la fecha, se han anillado algo más de 10.000.000 de aves y se han registrado 700.000 recuperaciones (en la actualidad se anillan unas 380.000 aves y se obtienen unas 30.000 recuperaciones anualmente). (2) En un segundo bloque se resume la aplicación del anillamiento en diferentes aproximaciones al estudio científico de las aves, que van apoyadas por numerosas referencias bibliográficas sobre trabajos llevados a cabo en España. Gracias al anillamiento se han podido abordar múltiples estudios sobre movimientos y migraciones, reproducción, demografía, enfermedades, morfología, muda e identificación y taxonomía. (3) Un tercer bloque se centra en la utilidad del anillamiento más allá de la investigación básica. Es el caso de la conservación, la gestión de especies cinegéticas y el estudio de los impactos del cambio global sobre las aves, por un lado, y la educación ambiental, formación y ciencia ciudadana, por otro. (4) Finalmente, se abordan algunas ideas sobre los retos actuales y perspectivas de futuro del anillamiento en España

Promoção de práticas alimentares saudáveis em pré-escolares de uma instituição educacional dacidade de Medellín

Food practices in schoolchildren show a high prevalence of sugar consumption. The objective of the study was to describe the prevalence of cavities, nutritional habits in preschoolers and first and to promote healthy eating practices at the school Ciudadela Nuevo Occidente. Materials and methods: A descriptive study was carried out with the participation of 61 boys and girls of the preschool, first and second grade, 80 parents or representatives and a non-participant observation was made to schoolchildren, school store and innkeepers, to explore the habits of food consumption in the Institution. Finally, a playful educational strategy was implemented that promoted healthy eating practices. A descriptive analysis of the information collected, and a bivariate analysis were carried out to compare the findings within the study period. The development study during the period 2017-II and 2019-II. Results: 60% of the respondents belonged to a level 1 socioeconomic stratum, 61% of parents had complete and incomplete secondary schooling. 58.8% have a daily consumption of sweets and soft drinks. The frequency of caries was 47.5% and white spots was 49.1%. Cariogenic foods are in the environment of children. 18 educational activities were carried out with 160 preschool and first school children and their teachers. Conclusions: practices such as high sugar intake, poor brushing, not packing children’s toothbrush for school,are risk factors that expose schoolchildren to cavities.Las prácticas alimentarias en escolares, muestran una alta prevalencia del consumo azucares. El objetivo del estudio fue describir la prevalencia de caries, los hábitos nutricionales en niños de preescolar y primero y fomentar prácticas alimentarias saludables en la Institución educativa Ciudadela Nuevo Occidente. Materiales y métodos: estudio descriptivo, con la participación de 61 niños y niñas del grado de preescolar y primero, 80 padres de familia o representantes y se realizó una observación no participante a los escolares, tienda escolar y venteros, para explorar los hábitos de consumo de alimentos en la Institución. Se realizó un análisis descriptivo de la información recolectada y un análisis bivariado para comparación de los hallazgos dentro del periodo de estudio. El estudio de desarrollo durante el periodo 2017-II y 2019-II. Resultados: el 60% de los encuestados pertenecía a un estrato socioeconómico nivel 1, el 61% de padres tenían escolaridad de secundaria completa e incompleta. 58,8% tiene un consumo diario de dulces y gaseosas. La frecuencia de caries fue del 47,5% y mancha blanca fue del 49,1%. Los alimentos cariogénicos están en el entorno de los niños. Se realizaron 18 actividades educativas con 160 niños de preescolar y primero y sus profesoras. Conclusiones: prácticas como el alto consumo de azúcar, un cepillado deficiente, no empacar a los niños el cepillo de dientes para el colegio, son factores de riesgo que exponen a los escolares a la aparición de la caries.As práticas alimentares em escolares mostram alta prevalência de consumo de açúcar. O objetivo do estudo foi descrever a prevalência de cáries, hábitos nutricionais em crianças pré-escolares e da primeira série e promover práticas alimentares saudáveis na instituição de ensino Ciudadela Nuevo Oeste. Materiais e métodos: estudo descritivo, com a participação de 61 meninos e meninas da pré-escola e primeira série, 80 pais ou representantes e foi realizada observação não participante dos escolares, loja escolar e lojistas, para explorar hábitos de consumo de alimentos. na Instituição. Foi realizada análise descritiva das informações coletadas e análise bivariada para comparação dos achados no período do estudo. O estudo de desenvolvimento durante o período 2017-II e 2019-II. Resultados: 60% dos entrevistados pertenciam a um estrato socioeconômico de nível 1, 61% dos pais tinham ensino médio completo ou incompleto. 58,8% consomem doces e refrigerantes diariamente. A frequência de cárie foi de 47,5% e de mancha branca foi de 49,1%. Os alimentos cariogênicos estão no ambiente infantil. Foram realizadas 18 atividades educativas com 160 crianças da pré-escola e da primeira série e seus professores. Conclusões: práticas como alto consumo de açúcar, má escovação e não levar as escovas de dente das crianças para a escola são fatores de risco que expõem os escolares ao aparecimento de cáries.