True and false DRM memories: differences detected with an implicit task

Memory is prone to illusions. When people are presented with lists of words associated with a non-presented critical lure, they produce a high level of false recognitions (false memories) for non-presented related stimuli indistinguishable, at the explicit level, from presented words (DRM paradigm). We assessed whether true and false DRM memories can be distinguished at the implicit level by using the autobiographical IAT (aIAT), a novel method based on indirect measures that permits to detect true autobiographical events encoded in the respondent's mind/brain. In our experiment, after a DRM task participants performed two aIATs: the first aimed at testing implicit memory for presented words (true-memories aIAT) and the second aimed at evaluating implicit memory for critical lures (false-memories aIAT). Specifically, the two aIATs assessed the association of presented words and critical lures with the logical dimension "true." Results showed that the aIAT detected a greater association of presented words than critical lures with the logical dimension "true."This result indicates that although true and false DRM memories are indistinguishable at the explicit level a different association of the true and false DRM memories with the logical dimension "true" can be detected at the implicit level, and suggests that the aIAT may be a sensitive instrument to detect differences between true and false DRM memories. © 2012 Marini, Agosta, Mazzoni, Dalla Barba and Sartori

DAily time use, Physical Activity, quality of care and interpersonal relationships in patients with Schizophrenia spectrum disorders (DiAPASon): an Italian multicentre study

Background: Schizophrenia spectrum disorders (SSD) are ranked among the leading causes of disabilities worldwide. Many people with SSD spend most of their daily time being inactive, and this is related to the severity of negative symptoms. Here, we present the 3-year DiAPAson project aimed at (1) evaluating the daily time use among patients with SSD living in Residential Facilities (RFs) compared to outpatients with SSD and to the general population (Study 1); (2) evaluating the quality of staff-patient relationships, its association with specific patient outcomes and the quality of care provided in RFs (Study 2); and (3) assessing daily activity patterns in residential patients, outpatients with SSD and healthy controls using real-time methodologies (Study 3). Methods: Study 1 will include 300 patients with SSD living in RFs and 300 outpatients; data obtained in these clinical populations will be compared with normative data obtained by the National Institute of Statistics (ISTAT) in the national survey on daily time use. Time use assessments will consist of daily diaries asking participants to retrospectively report time spent in different activities. In Study 2, a series of questionnaires will be administered to 300 residential patients (recruited for Study 1) to evaluate the quality of care and staff-patient relationships, level of well-being and burnout of RFs' staff, and quality of RFs using a European standardized questionnaire (QuIRC-SA). In Study 3, the daily time use will be evaluated in a subgroup of 50 residential patients, 50 outpatients and 50 healthy controls using the Experience Sampling Method approach (participants will complete a brief questionnaire -about time use, mood and perceived energy- on a smartphone 8 times a day for 1 week) to compare retrospective and real-time reports. Moreover, their level of physical activity, sleep patterns, and energy expenditure will be monitored through a multi-sensor device. Discussion: This project is highly innovative because it combines different types of assessments (i.e., retrospective and real-time reports; multi-sensor monitoring) to trace an accurate picture of daily time use and levels of physical activity that will help identify the best therapeutic options promoting daily activities and physical exercise in patients with SSD. Trial registration: ISRCTN registry ID ISRCTN21141466

Prolonged Neuromodulation of Cortical Networks Following Low-Frequency rTMS and Its Potential for Clinical Interventions

Non-invasive brain stimulation safely induces persistent large-scale neural modulation in functionally connected brain circuits. Interruption models of repetitive transcranial magnetic stimulation (rTMS) capitalize on the acute impact of brain stimulation, which decays over minutes. However, rTMS also induces longer-lasting impact on cortical functions, evident by the use of multi-session rTMS in clinical population for therapeutic purposes. Defining the persistent cortical dynamics induced by rTMS is complicated by the complex balance of excitation and inhibition among functionally connected networks. Nonetheless, it is these neuronal dynamic responses that are essential for the development of new neuromodulatory protocols for translational applications. We will review evidence of prolonged changes of cortical response, tens of minutes following one session of low frequency rTMS over the cortex. We will focus on the different methods which resulted in prolonged behavioral and brain changes, such as the combination of brain stimulation techniques, and individually tailored stimulation protocols. We will also highlight studies which apply these methods in multi-session stimulation practices to extend stimulation impact into weeks and months. Our data and others’ indicate that delayed cortical dynamics may persist much longer than previously thought and have potential as an extended temporal window during which cortical plasticity may be enhanced

The Origin of Word-related Motor Activity

Conceptual processing of verbs consistently recruits the left posterior middle temporal gyrus (lpMTG). The left precentral motor cortex also responds to verbs, with higher activity for action than nonaction verbs. The early timing of this effect has suggested that motor features of words' meaning are accessed directly, bypassing access to conceptual representations in lpMTG. An alternative hypothesis is that the retrieval of conceptual representations in lpMTG is necessary to drive more specific, motor-related representations in the precentral gyrus. To test these hypotheses, we first showed that repetitive transcranial magnetic stimulation (rTMS) applied to the verb-preferring lpMTG site selectively impoverished the semantic processing of verbs. In a second experiment, rTMS perturbation of lpMTG, relative to no stimulation (no-rTMS), eliminated the action–nonaction verb distinction in motor activity, as indexed by motor-evoked potentials induced in peripheral muscles with single-pulse TMS over the left primary motor cortex. rTMS pertubation of an occipital control site, relative to no-rTMS, did not affect the action–nonaction verb distinction in motor activity, but the verb contrast did not differ reliably from the lpMTG effect. The results show that lpMTG carries core semantic information necessary to drive the activation of specific (motor) features in the precentral gyrus

Highly specific memory B cells generation after the 2nd dose of BNT162b2 vaccine compensate for the decline of serum antibodies and absence of mucosal IgA

Specific memory B cells and antibodies are a reliable read-out of vaccine efficacy. We analysed these biomarkers after one and two doses of BNT162b2 vaccine. The second dose significantly increases the level of highly specific memory B cells and antibodies. Two months after the second dose, specific antibody levels decline, but highly specific memory B cells continue to increase, thus predicting a sustained protection from COVID-19. We show that although mucosal IgA is not induced by the vaccination, memory B cells migrate in response to inflammation and secrete IgA at mucosal sites. We show that the first vaccine dose may lead to an insufficient number of highly specific memory B cells and low concentration of serum antibodies, thus leaving vaccinees without the immune robustness needed to ensure viral elimination and herd immunity. We also clarify that the reduction of serum antibodies does not diminish the force and duration of the immune protection induced by vaccination. The vaccine does not induce sterilizing immunity. Infection after vaccination may be caused by the lack of local preventive immunity because of the absence of mucosal IgA

Quality of residential facilities in Italy: satisfaction and quality of life of residents with schizophrenia spectrum~disorders

Background Recovery and human rights promotion for people with Schizophrenia Spectrum Disorders (SSDs) is fundamental to provide good care in Residential Facilities (RFs). However, there is a concern about rehabilitation ethos in RFs. This study aimed to investigate the care quality of Italian RFs, the quality of life (QoL) and care experience of residents with SSD. Methods Fourty-eight RFs were assessed using a quality assessment tool (QuIRC-SA) and 161 residents with SSD were enrolled. Seventeen RFs provided high intensity rehabilitation (SRP1), 15 medium intensity (SRP2), and 16 medium-low level support (SRP3). Staff-rated tools measured psychiatric symptoms and psychosocial functioning; user-rated tools assessed QoL and satisfaction with services. RFs comparisons were made using ANOVA and Chi-squared. Results Over two-thirds patients (41.5 y.o., SD 9.7) were male. Seventy-six were recruited from SRP1 services, 48 from SRP2, and 27 from SRP3. The lowest QuIRC-SA scoring was Recovery Based Practice (45.8%), and the highest was promotion of Human Rights (58.4%). SRP2 had the lowest QuIRC-SA ratings and SRP3 the highest. Residents had similar psychopathology (p = 0.140) and functioning (p = 0.537). SRP3 residents were more employed (18.9%) than SRP1 (7.9%) or SRP2 (2.2%) ones, and had less severe negative symptoms (p = 0.016) and better QoL (p = 0.020) than SRP2 residents. There were no differences in the RF therapeutic milieu and their satisfaction with care. Conclusions Residents of the lowest supported RFs in Italy had less severe negative symptoms, better QoL and more employment than others. The lowest ratings for Recovery Based Practice across all RFs suggest more work is needed to improve recovery

ENIGMA-anxiety working group : Rationale for and organization of large-scale neuroimaging studies of anxiety disorders

Altres ajuts: Anxiety Disorders Research Network European College of Neuropsychopharmacology; Claude Leon Postdoctoral Fellowship; Deutsche Forschungsgemeinschaft (DFG, German Research Foundation, 44541416-TRR58); EU7th Frame Work Marie Curie Actions International Staff Exchange Scheme grant 'European and South African Research Network in Anxiety Disorders' (EUSARNAD); Geestkracht programme of the Netherlands Organization for Health Research and Development (ZonMw, 10-000-1002); Intramural Research Training Award (IRTA) program within the National Institute of Mental Health under the Intramural Research Program (NIMH-IRP, MH002781); National Institute of Mental Health under the Intramural Research Program (NIMH-IRP, ZIA-MH-002782); SA Medical Research Council; U.S. National Institutes of Health grants (P01 AG026572, P01 AG055367, P41 EB015922, R01 AG060610, R56 AG058854, RF1 AG051710, U54 EB020403).Anxiety disorders are highly prevalent and disabling but seem particularly tractable to investigation with translational neuroscience methodologies. Neuroimaging has informed our understanding of the neurobiology of anxiety disorders, but research has been limited by small sample sizes and low statistical power, as well as heterogenous imaging methodology. The ENIGMA-Anxiety Working Group has brought together researchers from around the world, in a harmonized and coordinated effort to address these challenges and generate more robust and reproducible findings. This paper elaborates on the concepts and methods informing the work of the working group to date, and describes the initial approach of the four subgroups studying generalized anxiety disorder, panic disorder, social anxiety disorder, and specific phobia. At present, the ENIGMA-Anxiety database contains information about more than 100 unique samples, from 16 countries and 59 institutes. Future directions include examining additional imaging modalities, integrating imaging and genetic data, and collaborating with other ENIGMA working groups. The ENIGMA consortium creates synergy at the intersection of global mental health and clinical neuroscience, and the ENIGMA-Anxiety Working Group extends the promise of this approach to neuroimaging research on anxiety disorders

The autobiographical IAT: a new technique for memory detection

The autobiographical IAT (aIAT) is a new technique of memory detection that can be used to identify which of two autobiographical events is true. The technique is based on a classification task. Participants have to classify items in four different categories using only two motor responses. The underlying assumption is that the condition in which two associated concepts require the same motor response (congruent block) reaction times will be faster than the condition where the two associated concepts require two different motor responses (incongruent block). The practical applications of this technique to the forensic field are straightforward. Six validation studies have been run (Chapter 2). In all the six experiments the true autobiographical event has been identified on the basis of the pattern of reaction times (RTs) in fact the congruent block show faster RTs than the incongruent block. It has also been shown that coached participants can successfully fake the aIAT, but faking can be detected on the basis of a specific pattern of reaction times (Chapter 3). The accuracy and validity of the aIAT has been evaluated further and I showed that to enhance the validity of the instrument is necessary to be cautious in using sentences to describe autobiographical events (Chapter 4). Finally, it has been shown that the aIAT can be applied to the identification of intentions, other than autobiographical events (Chapter 5). The application of the aIAT to the intention detection has been investigated also with Event Related Potentials (ERPs). Results showed a reduced Late Positive Component (LPC) for the incongruent block in respect to the congruent one. The LPC has been shown to be related to the cognitive control indicating here a stronger cognitive control during the incongruent than the congruent block. In sum, the aIAT has been shown to be a reliable method that can be used to identify an autobiographical event or a future intention.Obiettivo della ricerca è stato quello di validare un nuovo strumento, l’Autobiographical Implicit Association Test (aIAT), basato su misure indirette, il cui scopo è verficare la veridicità di un evento autobiografico. L’aIAT, stabilisce l’associazione fra la descrizione verbale di un evento (e un contro-evento) e la dimensione logica vero-falso. Compito del soggetto è quello di classificare delle frasi, che vengono presentate al centro di uno schermo, il più velocemente possibile. Ai partecipanti vengono presentati in ordine casuale item relativi a quattro concetti, due concetti target (evento-contro evento) e la dimensione logica (vero-falso); il compito dei partecipanti è quello di classificare gli item mediante due tasti; le risposte possibili del soggetto sono solamente due, in modo tale che i quattro concetti vengano associati a coppie. L’assunto di base prevede che i partecipanti siano più veloci nel compito di classificazione quando i concetti associati richiedono la medesima risposta (compito congruente). Al contrario, quando i due concetti associati richiedono risposte differenti, i tempi di reazione saranno relativamente più lenti (compito incongruente). Tale procedura è stata validata mediante una serie di esperimenti (Capitolo 2) il cui scopo era discriminare: -quale fra due carte è stata scelta da un partecipante, aIAT carte. -fra due gruppi di partecipanti, coloro che hanno commesso un crimine da partecipanti che hanno letto un articolo di giornale, aIAT mock crime -fra soggetti a cui è stata ritirata la patente per guida in stato d’ebbrezza e soggetti a cui non è mai stata ritirata la patente, aIAT guida in stato d’ebbrezza. I tempi di reazione delle situazioni congruenti sono significativamente più veloci dei tempi di reazione delle situazioni incongruenti. Studi successivi (Capitolo 3) hanno dimostrato come sia possibile utilizzare delle contromisure al test, ma queste stesse contromisure possono essere facilmente rintracciabili sulla base del pattern di tempi di reazione. Un ulteriore milgioramento del test è stato effettuaato attraverso alcuni studi (Capitolo 4) che hanno dimostrato che l’utilizzo di frasi ed etichette nella forma negativa riduca l’accuratezza del test. Infine l’aIAT è stato applicato allo studio delle intenzioni (Capitolo 5) e si è dimostrato in grado di individuare non solo gli eventi autobiografici accaduti in passato, ma anche le intenzioni future. Lo studio delle intenzioni è stato effettuato anche mediante la tecnica dei potenziali evocati che ha identificato una diversa componente tardiva (late positive component, LPC) nel blocco congruente ed incongruente, in particolare si è trovata una LPC ridotta nel blocco incongruente rispetto a quello congruente. La riduzione della LPC è stato associato in letteratura al controllo cognitivo, indicando quindi la necessità di un maggiore controllo mentre il partecipante svolge il blocco incongruente

The autobiographical IAT: a review

The autobiographical Implicit Association Test (aIAT; Sartori et al., 2008) is a variant of the Implicit Association Test (IAT; Greenwald et al., 1998) that is used to establish whether an autobiographical memory is encoded in the respondent's mind/brain. More specifically, with the aIAT, it is possible to evaluate which one of two autobiographical events is true. The method consists of a computerized categorization task. The aIAT includes stimuli belonging to four categories, two of them are logical categories and are represented by sentences that are always true (e.g., I am in front of a computer) or always false (e.g., I am climbing a mountain) for the respondent; two other categories are represented by alternative versions of an autobiographical event (e.g., I went to Paris for Christmas, or I went to New York for Christmas), only one of which is true. The true autobiographical event is identified because, in a combined block, it gives rise to faster reaction times when it shares the same motor response with true sentences. Here, we reviewed all the validation experiments and found more than 90% accuracy in detecting the true memory. We show that agreement in identifying the true autobiographical memory of the same aIAT repeated twice is, on average, more than 90%, and we report a technique for estimating accuracy associated with a single classification based on the D-IAT value, which may be used in single subject's investigations. We show that the aIAT might be used to identify also true intentions and reasons and conclude with a series of guidelines for building an effective aIAT