Relatório de estágio profissionalizante em medicina comunitária

Mestrado Integrado em MedicinaMaster Degree in Medicin

‘A true feeling of authentic portuguese golf'? The discursive construction of the Algarve region in portuguese golf websites

In an increasingly competitive global marketplace, the need for golf destinations to differentiate themselves from competitors has become more critical than ever. This paper raises questions about the promotional strategies employed by the golf sector in the Algarve, focusing on internet communication strategies, since this medium has become the biggest driving force towards the commoditisation of all aspects of the tourism experience. By offering a complementary perspective to the field of (critical) tourism studies, and drawing on a qualitative, multi-modal discourse analysis, this work-in-progress looks at the particular ways that representations and images presented on the Algarve golf websites constitute and frame identities (of people and places) and socio-spatial relationships. This paper analyses a corpus of 45 texts collected from official websites of the 40 Algarve golf courses and from five entities which promote the Algarve as a golf destination, along with the golf images that are displayed alongside them. Findings point to salient discursive and visual representations of a global setting enjoyed by the global elite. Whereas the courses‟ positioning in relation to their regional competitors draws on similar discursive strategies which reflect those used in tourism advertising discourses in general – e.g. reiteration of explicit comparisons, superlatives and hyperbolic statements -, representations of local emplacedness are not salient; in some cases local place seems to have been almost intentionally suppressed

Analysis of a vegetable oil performance in a milling process by MQL lubrication

In this work, we carried out a comparison between the dry machining of an aluminum block with conventional cutting oil and a block with vegetable oil. The two oils had different flow rates. Using the Taguchi method, it was possible to determine the matrices for optimizing the best parameters for each group of tests. Then, we studied the utility of using vegetable oil as a cutting lubricant. We found that the vegetable oil studied in this work had good properties in terms of reducing cutting temperatures but was less effective than conventional cutting oil in reducing the surface roughness of the machined part. Tribological tests were carried out to understand the influence of the selected lubricants in reducing friction and wear. After the sliding experiments, which were performed without lubrication in the presence of the same lubricants that were used in the machining tests and in the presence of distilled water, we concluded that vegetable oil has satisfactory lubricating properties that are similar to those of the conventional cutting fluid, indicating a potential for consideration as an effective alternative to the conventional cutting fluid, with economic, environmental, and health advantages.Financial support was provided by Portugal’s national funding FCT/MCTES (PIDDAC) to Centro de Investigação de Montanha (CIMO) (UIDB/00690/2020 and UIDP/00690/2020) and SusTEC (LA/P/0007/2020). Additionally, this work was partially supported by Portuguese FCT, under the reference projects UIDB/04077/2020, UIDB/00532/2020 and UIDB/04436/2020. This research was also partially funded by EXPL2021CIMO_01. Inês Afonso acknowledges the financial support of CIMO through EXPL2021CIMO_01.info:eu-repo/semantics/publishedVersio

Characterization of PlyPl23, an endolysin from a Paenibacillus larvae bacteriophage

EMBO Conference on Viruses of Microbes III: Structure and Function - from Molecules to Communities (Programme and Abstract Book)Bees pollination presents a great impact in a wide range of terrestrial ecosystems. The estimated world value for the contribution of pollinators, especially bees, to the production of crops used directly for human food was estimated in 153 billion. Yet, beekeeping is facing many challenges, as changes in agricultural practices, extensive use of pesticides and the presence of pathogens. One of the most widespread and the most destructive of the honeybee brood diseases is the American foulbrood (AFB), caused by Paenibacillus larvae, a Gram-positive bacterium wherein the spore is the infectious form. In most situations P. larvae is highly virulent and when contracted will kill a complete colony. This represents an economic threat to commercial beekeepers and apiculture worldwide. The European regulation limits the presence of antibiotics in honey, excluding its use for therapy. The antibacterial features of (bacterio)phages and endolysins were considered for the development of a therapeutic product for this disease. A P. larvae phage, phiIBB_Pl23, belonging to the Siphoviridae family was isolated and its genome has been sequenced and analyzed. Annotation of the phiIBB_Pl23 genome identified gp21 as an endolysin protein, designated as PlyPl23, which was further characterized and is presented herein. In silico analysis revealed that PlyPl23, is a single domain globular protein, which is a rare feature of Gram-positive endolysins. The catalytic domain was identified as an N-acetylmuramoyl-L-alanine amidase (pfam Amidase_2 family) with a molecular weight of 25.8 kDa and isoelectric point of 5.74. Activity tests were performed at 37oC, which is similar to the hive temperature (about 34-35ºC) and revealed that this is a broad-spectrum endolysin for P. larvae with an optimal pH for reactivity between 3 and 5. This matches the pH levels of honey, nectar, pollen, and royal jelly (pH 3 to 4). The enzyme also displays a high antimicrobial activity between 5 and 7 when supplemented with NaCl, matching also the intestinal pH of bee larvae (pH 6.8) and adult bees (pH 5.6 to 6.3). Furthermore, tests performed with honeybee larvae juice revealed a higher bacterial decrease reduction, which may be a good indicator for the in vivo effectiveness of the endolysin. The present work describes, to our knowledge, the first characterization of an endolysin from a P. larvae phage. Moreover, characterization of endolysin PlyPl23 showed that it presents high potential in the development of a commercial product to control the problematic AFB.info:eu-repo/semantics/publishedVersio

Right ventricular lead in cardiac resynchronization therapy : what is the most electrically favorable stimulation site?

© The European Society of Cardiology 2018. All rights reserved.Introduction: The most recent studies in cardiac resynchronization therapy (CRT) have been focused on attempts to improve response rate, such as the determination of the optimal placement of the left ventricular (LV) lead. The position of the LV lead guided by the site of latest electrical delay (ED), seems to be a promising strategy. The same strategy may be useful in assessing the ideal positioning of right ventricular (RV) lead in CRT. Purpose: To determine the most favorable position of the RV lead (septal versus apical) in CRT, by measuring the ED of the LV poles during septal or apical RV pacing in patients with triple-site CRT (TRIV). Methods: A single-center prospective study of consecutive patients (Oct. 2014 to Oct. 2017) submited to CRT device implantation (Quadra Allure MPTM, which allows post-implantation ED measurement) in TRIV mode - with a quadripolar lead in the LV, a RV lead positioned at the septum and a RV lead at the apex. In the follow-up, the lead position was confirmed and determined by fluoroscopic evaluation (anterior, lateral and postero-lateral in the short axis and basal, mid and apical on the long axis). The ED was measured at the 4-pole LV lead, during apical and septal RV pacing and the relation between the ED and lead position was evaluated. Results: Twenty two patients were included: 82% male, median age 78 years; 50% implanted CRT with defibrillator; 27% had ischemic heart disease and 73% non-ischemic etiology; all patients had permanent atrial fibrillation and the QRS duration was of 176±29ms. The position of the LE lead poles was classified as lateral in 44% and postero-lateral in 56%, basal in 28%, medial in 39% and apical in 34%. We evaluated 160 ED. The mean ED between the apical RV lead and the LV poles was significantly higher than the delay between septal RV lead and the LV poles [161±33ms vs 75±45ms (p<0.001)]. This difference remained significant in LV poles located in a lateral position (76±62 vs. 166±36ms; p<0.001) or in a postero-lateral position (74±30 vs. 157±31ms; p<0.001); the same ocurred for LV poles in a basal (99±62 vs. 179±24ms, p<0.001), mid (72±38 vs. 167±31ms, p<0.001) and apical location (57±24 vs. 140±32ms, p<0.001). Conclusion: The LV lead position is an important determinant of the success of CRT, but is conditioned by anatomical and technical characteristics. The optimization of CRT may then depend on the positioning of RV lead. This study demonstrated that the apical (vs. septal) RV lead positioning presents higher ED in relation to LV lead, regardless of the latter position.info:eu-repo/semantics/publishedVersio

Analysis of a vegetable oil performance in a milling process by MQL Lubrication

In this work, we carried out a comparison between the dry machining of an aluminum block with conventional cutting oil and a block with vegetable oil. The two oils had different flow rates. Using the Taguchi method, it was possible to determine the matrices for optimizing the best parameters for each group of tests. Then, we studied the utility of using vegetable oil as a cutting lubricant. We found that the vegetable oil studied in this work had good properties in terms of reducing cutting temperatures but was less effective than conventional cutting oil in reducing the surface roughness of the machined part. Tribological tests were carried out to understand the influence of the selected lubricants in reducing friction and wear. After the sliding experiments, which were performed without lubrication in the presence of the same lubricants that were used in the machining tests and in the presence of distilled water, we concluded that vegetable oil has satisfactory lubricating properties that are similar to those of the conventional cutting fluid, indicating a potential for consideration as an effective alternative to the conventional cutting fluid, with economic, environmental, and health advantages.Financial support was provided by Portugal’s national funding FCT/MCTES (PIDDAC) to Centro de Investigação de Montanha (CIMO) (UIDB/00690/2020 and UIDP/00690/2020) and SusTEC (LA/P/0007/2020). Additionally, this work was partially supported by Portuguese FCT, under the reference projects UIDB/04077/2020, UIDB/00532/2020 and UIDB/04436/2020. This research was also partially funded by EXPL2021CIMO_01. Inês Afonso acknowledges the financial support of CIMO through EXPL2021CIMO_01

DEMOCRACIA, NEOLIBERALISMO E POLÍTICAS PÚBLICAS NO VELHO E NOVO MUNDO: desafios para o século XXI

Este texto ressalta a natureza de classe, os aspectos políticos e ideológicos típicos de algumas democracias européias e latinoamericanas e os desafios dos sujeitos sociais em luta contra os processos estruturais do capitalismo, geradores de exploração e desigualdades sociais. Destacamos em Portugal o desenvolvimento das políticas públicas, sobretudo após a revolução democrática de Abril de 1974 até o momento atual, dando particular relevo à Educação. Na América Latina, trazemos as lutas sociais do proletariado em confronto com as ambigüidades da democracia burguesa. No Brasil, discutiremos as relações entre os movimentos sociais, as ONGs, as políticas públicas e os rebatimentos na democracia

Determination of minimum inhibitory concentrations of several plants extracts against different bacterial pathogens

In the context of COVID 19 the use of face masks has been recommended as a preventive measure against the spread of SARS CoV 2 1 Despite their health benefits, usage of single use masks represents a threat to the environment as they are manufactured fromlong lasting plastic materials Reusable fabric masks are an alternative to reduce the risk of pollution and the amount of plastic wastage 2. The application of antimicrobial agents in the tissues used to produce masks would be an additional hurdle on the preventionof other respiratory infections and secondary bacterial infections that occur from touching contaminated masks In this context, impregnation of the fabrics with plant extracts is an attractive approach since they are potentially safe and free of adverse side effectsand powerful antimicrobials. The objective of this work was to study several plant extracts to select the most efficient against pathogenic microorganisms.info:eu-repo/semantics/publishedVersio

Evaluation of minimum inhibitory concentrations of plants extracts against several bacterial pathogens

info:eu-repo/semantics/publishedVersio