Climate change adaptation and its implementation at national and international levels

Climate change adaptation is the second arm of climate change action and seeks to ensure that all countries tackle the impacts of climate change by addressing both social and geographical vulnerabilities. The article discusses adaptation and its application at national and international levels. It also examines adaptation to climate change as a developmental challenge and how the principles of sustainable development can aid climate change action. The article analyses relevant aspects of national and international instruments relating to climate change adaptation and spotlights the commitment of Nigeria and South Africa to climate change adaptation. The article finds that integrating sustainable development into governance helps adaptation and increases resilience against the impacts of climate change. The article also emphasizes the necessity of good governance, particularly protection of human rights, climate finance and effective land use governance, to ensure climate change adaptation. The article argues that climate change adaptation should be viewed more as a developmental, rather than an environmental, challenge. It further asserts that coordinated action, especially in relation to land use governance and sustainable development is required to ensure sustainable climate action. This is particularly so given that citizens of many developing countries, including Nigeria and South Africa, depend on land and its resources  for social and economic development. Keywords: Adaptation; Climate Finance; Good Governance; Nigeria; South Africa

An outline and critical assessment of the role of planning laws in the regulatory framework of climate change adaptation in South Africa and Nigeria

Climate change affects the natural and built environment, including all forms of development. The risks of climate change include severe alterations to the normal functioning of communities, including adverse effects on humans, infrastructure, land use, the built and natural environment. Due to these occurring and projected adverse effects, there is the need to develop resilience within communities, especially in areas of particular vulnerability. The point of departure of this research is that planning laws have a role to play in the adapting to, and building of resilience against, climate change. As such the work argues for an integration of climate change considerations into planning, environmental and related laws. The relevant laws will be examined to consider the extent to which they are suited to aid the adaptation process. The research entails a comparative approach through the analysis of planning laws in Nigeria and South Africa. It also involves qualitative empirical research into the effectiveness of planning laws as a means of adaptation to climate change. Planning law in Nigeria is very procedural and is limited to physical planning and ordering of the built environment. South Africa, on the other hand, has integrated sustainable development considerations into her planning legislation and it now encompasses spatial planning, integrated development plans, land use management and others. In both countries, however, there seems to be no express integration of climate change into planning laws, especially at the national level of government. The research also examines the legal aspects of climate change adaptation strategies in both countries. It found that in Nigeria in particular, there is no provision for inter-governmental cooperation to facilitate physical planning or climate adaptation action. In the case of South Africa, there is a well-structured intergovernmental collaboration regarding planning and land use management, which gives some consideration to climate change adaptation. However, the structure for cooperation is still being developed as seen in the draft National Adaptation Strategy and the proposed Climate Change Act. There is a need to reevaluate the relevant laws in both countries, with special consideration to the role of planning in adaptation to the adverse impacts of climate change on the natural and built environment

Recording long-term potentiation of synaptic transmission by three-dimensional multi-electrode arrays

BACKGROUND: Multi-electrode arrays (MEAs) have become popular tools for recording spontaneous and evoked electrical activity of excitable tissues. The majority of previous studies of synaptic transmission in brain slices employed MEAs with planar electrodes that had limited ability to detect signals coming from deeper, healthier layers of the slice. To overcome this limitation, we used three-dimensional (3D) MEAs with tip-shaped electrodes to probe plasticity of field excitatory synaptic potentials (fEPSPs) in the CA1 area of hippocampal slices of 129S5/SvEvBrd and C57BL/6J-Tyr(C-Brd )mice. RESULTS: Using 3D MEAs, we were able to record larger fEPSPs compared to signals measured by planar MEAs. Several stimulation protocols were used to induce long-term potentiation (LTP) of synaptic responses in the CA1 area recorded following excitation of Schäffer collateral/commissural fibres. Either two trains of high frequency tetanic stimulation or three trains of theta-burst stimulation caused a persistent, pathway specific enhancement of fEPSPs that remained significantly elevated for at least 60 min. A third LTP induction protocol that comprised 150 pulses delivered at 5 Hz, evoked moderate LTP if excitation strength was increased to 1.5× of the baseline stimulus. In all cases, we observed a clear spatial plasticity gradient with maximum LTP levels detected in proximal apical dendrites of pyramidal neurones. No significant differences in the manifestation of LTP were observed between 129S5/SvEvBrd and C57BL/6J-Tyr(C-Brd )mice with the three protocols used. All forms of plasticity were sensitive to inhibition of N-methyl-D-aspartate (NMDA) receptors. CONCLUSION: Principal features of LTP (magnitude, pathway specificity, NMDA receptor dependence) recorded in the hippocampal slices using MEAs were very similar to those seen in conventional glass electrode experiments. Advantages of using MEAs are the ability to record from different regions of the slice and the ease of conducting several experiments on a multiplexed platform which could be useful for efficient screening of novel transgenic mice

Reciprocal Effects on Neurocognitive and Metabolic Phenotypes in Mouse Models of 16p11.2 Deletion and Duplication Syndromes.

The 16p11.2 600 kb BP4-BP5 deletion and duplication syndromes have been associated with developmental delay; autism spectrum disorders; and reciprocal effects on the body mass index, head circumference and brain volumes. Here, we explored these relationships using novel engineered mouse models carrying a deletion (Del/+) or a duplication (Dup/+) of the Sult1a1-Spn region homologous to the human 16p11.2 BP4-BP5 locus. On a C57BL/6N inbred genetic background, Del/+ mice exhibited reduced weight and impaired adipogenesis, hyperactivity, repetitive behaviors, and recognition memory deficits. In contrast, Dup/+ mice showed largely opposite phenotypes. On a F1 C57BL/6N × C3B hybrid genetic background, we also observed alterations in social interaction in the Del/+ and the Dup/+ animals, with other robust phenotypes affecting recognition memory and weight. To explore the dosage effect of the 16p11.2 genes on metabolism, Del/+ and Dup/+ models were challenged with high fat and high sugar diet, which revealed opposite energy imbalance. Transcriptomic analysis revealed that the majority of the genes located in the Sult1a1-Spn region were sensitive to dosage with a major effect on several pathways associated with neurocognitive and metabolic phenotypes. Whereas the behavioral consequence of the 16p11 region genetic dosage was similar in mice and humans with activity and memory alterations, the metabolic defects were opposite: adult Del/+ mice are lean in comparison to the human obese phenotype and the Dup/+ mice are overweight in comparison to the human underweight phenotype. Together, these data indicate that the dosage imbalance at the 16p11.2 locus perturbs the expression of modifiers outside the CNV that can modulate the penetrance, expressivity and direction of effects in both humans and mice

In vivo monitoring of neuronal loss in traumatic brain injury: a microdialysis study

Traumatic brain injury causes diffuse axonal injury and loss of cortical neurons. These features are well recognized histologically, but their in vivo monitoring remains challenging. In vivo cortical microdialysis samples the extracellular fluid adjacent to neurons and axons. Here, we describe a novel neuronal proteolytic pathway and demonstrate the exclusive neuro-axonal expression of Pavlov’s enterokinase. Enterokinase is membrane bound and cleaves the neurofilament heavy chain at positions 476 and 986. Using a 100 kDa microdialysis cut-off membrane the two proteolytic breakdown products, extracellular fluid neurofilament heavy chains NfH476−986 and NfH476−1026, can be quantified with a relative recovery of 20%. In a prospective clinical in vivo study, we included 10 patients with traumatic brain injury with a median Glasgow Coma Score of 9, providing 640 cortical extracellular fluid samples for longitudinal data analysis. Following high-velocity impact traumatic brain injury, microdialysate extracellular fluid neurofilament heavy chain levels were significantly higher (6.18 ± 2.94 ng/ml) and detectable for longer (>4 days) compared with traumatic brain injury secondary to falls (0.84 ± 1.77 ng/ml, <2 days). During the initial 16 h following traumatic brain injury, strong correlations were found between extracellular fluid neurofilament heavy chain levels and physiological parameters (systemic blood pressure, anaerobic cerebral metabolism, excessive brain tissue oxygenation, elevated brain temperature). Finally, extracellular fluid neurofilament heavy chain levels were of prognostic value, predicting mortality with an odds ratio of 7.68 (confidence interval 2.15–27.46, P = 0.001). In conclusion, this study describes the discovery of Pavlov’s enterokinase in the human brain, a novel neuronal proteolytic pathway that gives rise to specific protein biomarkers (NfH476−986 and NfH476−1026) applicable to in vivo monitoring of diffuse axonal injury and neuronal loss in traumatic brain injury

Evolution of GluN2A/B cytoplasmic domains diversified vertebrate synaptic plasticity and behavior

Two genome duplications early in the vertebrate lineage expanded gene families, including GluN2 subunits of the NMDA receptor. Diversification between the four mammalian GluN2 proteins occurred primarily at their intracellular C−terminal domains (CTDs). To identify shared ancestral functions and diversified subunit−specific functions, we exchanged the exons encoding the GluN2A (also known as Grin2a) and GluN2B (also known as Grin2b) CTDs in two knock−in mice and analyzed the mice's biochemistry, synaptic physiology, and multiple learned and innate behaviors. The eight behaviors were genetically separated into four groups, including one group comprising three types of learning linked to conserved GluN2A/B regions. In contrast, the remaining five behaviors exhibited subunit−specific regulation. GluN2A/B CTD diversification conferred differential binding to cytoplasmic MAGUK proteins and differential forms of long−term potentiation. These data indicate that vertebrate behavior and synaptic signaling acquired increased complexity from the duplication and diversification of ancestral GluN2 gene

Mouse models of 17q21.31 microdeletion and microduplication syndromes highlight the importance of Kansl1 for cognition

Koolen-de Vries syndrome (KdVS) is a multi-system disorder characterized by intellectual disability, friendly behavior, and congenital malformations. The syndrome is caused either by microdeletions in the 17q21.31 chromosomal region or by variants in the KANSL1 gene. The reciprocal 17q21.31 microduplication syndrome is associated with psychomotor delay, and reduced social interaction. To investigate the pathophysiology of 17q21.31 microdeletion and microduplication syndromes, we generated three mouse models: 1) the deletion (Del/+); or 2) the reciprocal duplication (Dup/+) of the 17q21.31 syntenic region; and 3) a heterozygous Kansl1 (Kans1+/-) model. We found altered weight, general activity, social behaviors, object recognition, and fear conditioning memory associated with craniofacial and brain structural changes observed in both Del/+ and Dup/+ animals. By investigating hippocampus function, we showed synaptic transmission defects in Del/+ and Dup/+ mice. Mutant mice with a heterozygous loss-of-function mutation in Kansl1 displayed similar behavioral and anatomical phenotypes compared to Del/+ mice with the exception of sociability phenotypes. Genes controlling chromatin organization, synaptic transmission and neurogenesis were upregulated in the hippocampus of Del/+ and Kansl1+/- animals. Our results demonstrate the implication of KANSL1 in the manifestation of KdVS phenotypes and extend substantially our knowledge about biological processes affected by these mutations. Clear differences in social behavior and gene expression profiles between Del/+ and Kansl1+/- mice suggested potential roles of other genes affected by the 17q21.31 deletion. Together, these novel mouse models provide new genetic tools valuable for the development of therapeutic approaches.PMC553161

Novel Doubly Salient Stator Slot Permanent Magnet Electrical Machines

It is well known that the torque density in electrical machines is limited by magnetic saturation and thermal constraints on the winding insulation and permanent magnets (PMs). In particular, doubly salient electrically-excited machines with the armature and DC windings on the stator, for example, the variable-flux machine (VFM), have (a) limited stator slot area for both windings, (b) severe magnetic saturation due to the DC excitation, and (c) negligible reluctance torque, and may exhibit high copper loss and high magnetic saturation. A novel method to alleviate magnetic saturation in the stator of VFMs is proposed. It is achieved by pre-magnetizing the stator core using PMs placed in the stator slots. The PM effects and performance improvements are analyzed by the finite element method based on the frozen permeability (FP) method. The developed novel VFM with PMs is a hybrid excited stator slot PM (HSSPM) machine. The basic operation principle and the electromagnetic performance with different stator and rotor pole combinations having double (all pole wound concentrated windings) and single layer (alternate pole wound concentrated windings) windings are investigated. HSSPM machines have improved electromagnetic performance and also retain the good flux regulation capability of VFMs. They can also be operated without the DC excitation, i.e. only with the armature and PM excitation. Thus, a new machine configuration – stator slot PM machine (SSPM) is further developed and investigated in terms of the influence of stator/rotor pole combinations and double and single layer windings. The newly developed SSPM machine also has the potential for fail-safe capability in the event of drive faults, and in addition, the requirements on the DC inverter and controller can be eliminated. The finite element predicted electromagnetic performances of HSSPM and SSPM machines are experimentally validated. Finally, the PM- and iron losses in HSSPM and SSPM machines having different stator/rotor pole combinations with single and double layer windings and their demagnetization ratio are analyzed. The influence of leading design parameters on their machine losses and efficiency is further investigated and their electromagnetic performance compared

Sustainable housing through urban renewal in contemporary Lagos

A research report submitted to the faculty of engineering and the built environment, University of Witwatersrand, in fulfilment of the requirements for the degree of Master of built environment.Lagos, like most cities in developing countries, is experiencing a continuous decline in its built environment and urban fabric. This decline in the city, especially regarding human settlements implies an insufficiency of houses and overburdening of already limited infrastructure giving rise to urban sprawl, slum proliferation and informal settlements. These challenges have led to the formulation of housing and urban renewal policies and strategies towards revitalising the city. This research aims to explore how urban renewal can be used to achieve sustainable housing in line with the sustainable development goal for cities. The research assesses existing policies and legislative frameworks that govern urban renewal and implementation within the state. Also, this research examines the role of the government in providing houses for the populace, especially, the urban poor. The research adopts a qualitative approach to address the research questions. With the use of semi-structured interviews, the researcher collected data through a purposeful sampling of participants in the field of urban renewal and housing development in Lagos state. A case of Isale Gangan Towers and Gardens by Lagos State Urban Renewal Agency was examined as an intervention involving urban renewal and housing provision.This research gives an insight into the level of government’s responsiveness to renewing slums and provision of housing. The research reveals that the task of housing delivery in Lagos is enormous and government cannot address the challenges alone and equally renew slums and informal settlements, hence, the need for solutions that are co-produced. The research also shows that the city of Lagos, like many cities in developing countries, partially caters for the urban poor, and a consequence of government’s inaction in this regard is the proliferation of slums and informal settlements. Finally, that housing provision can be complemented with urban renewal of slums to achieve sustainable housing.GR201