High Tunnel Design, Site Development, and Construction

High tunnels are low technology plant growing structures that provide opportunity for season extension, severe weather protection, and modified environments to increase productivity and visual quality of harvested plant products. This publication describes high tunnel components, site selection, development, covering materials, installation, and operation. Experienced specialty plant growers can increase the quality of their products and the duration of their marketing of high- value crops through the use of high tunnels. High tunnels give growers the opportunity to plant earlier and include more sequential planting dates. This can result in early- season, high- dollar returns and the ability to offer their products for longer periods. High tunnels also provide environmental protection, which helps reduce blemishes and discoloration, improving visual appeal. For successful high tunnel crop production, decisions and actions before planting the first crop include choosing a structural design to meet specific needs, identifying the best site for locating the structure, initial soil preparation, and the method of construction

The Biggest Grower - A youth gardening competition for growing specialty crops and urban farmers

Youth today have tendencies for unhealthy lifestyles, being sedentary, consuming high fat diets low in fruits and vegetables, all contributing to child obesity, type 2 diabetes, hypertension, and precedence for life-long health concerns. School lunch programs provide opportunity for youth to consume balanced diets but does not make a significant change in lifestyle. Research has identified that youth participating in gardening at home were positively impacted with making lasting healthy choices by improved knowledge and garden connection. For rural and economically disadvantaged urban households, poor diet is directly attributed to the inaccessibility of fresh produce, affordability and understanding of preparation of various specialty crops. Through a grant from the NE Specialty Crop Block Funding Program, The Biggest Grower Competition (BGC) was developed as a summer extension education program for youth in 8th through 12th grade. Project goals included; provide educational materials on gardening, benefits of consuming fresh edibles, and entrepreneurship opportunities; to have participants grow their own crops, collect productivity data, record method of distribution; and, measure behavioral changes in consumption of specialty crops for individual and family. Programs were completed at participants individually during the period of June 1 – August 12 in each of 2020 and 2021. Each developed their own 8 m2 garden (or container collection) and participated in 10 weekly asynchronous virtual educational modules (1 hour per week) and routine synchronous virtual meetings with their Garden Mentor. Participants were invited to come to the University of Nebraska-Lincoln campus (2021) on week 4 for a one-day, no cost program with engagement activities, garden tours, exploration of agriculture technology, and to network with agriculture specialists. Incentives for participation included receiving a multi-action gardening tool, educational book, assorted seeds, colorful logo stickers, and an official contestant t-shirt. Records were maintained of harvest poundage and distribution method. The top eight producers received gift cards in varying amounts as an incentive for ongoing participation and complete submission of data. In 2020, there were 44 participants in which 57% were new to gardening and, for 2021, 17 participants in which 25% were new gardeners. Yearly individual garden production averages were 21.55 kg and 23.13 kg respectively with a total of 1,118.56 kg of fresh produce grown. Through a post-experience survey, daily produce consumption rate increased from 12.5% to 36.4%, 72% of the participants tried a new vegetable/fruit recipe, 91% indicated produce grown was consumed by family, and 18% was donated to food banks

Evaluating the Impact of Grafting on Local Tomato Production in Nebraska

Grafting has been successfully used in vegetable production for tomatoes, peppers, eggplants, cucumbers, and watermelon. Besides its usefulness for managing soil-borne diseases, grafting can improve nutrient uptake and yield. However, few studies have assessed the effects of grafting and soil fertility management on yield of open field-grown tomatoes in the Midwest. Therefore, the objective of this two-year research was to better document the effects of grafting heirloom and hybrid tomato cultivar onto hybrid tomato rootstocks on tomato yield and quality. The field experiments were located at the University of Nebraska Lincoln - East Campus in Lincoln, West Central Research and Extension Center in North Platte, and a farm trial at Perkarek’s Vegetable Farm near Dwight, Nebraska. Two determinant fresh market tomatoes, ‘Nebraska Wedding’ and ‘BHN-589’, were grafted onto two rootstocks, ‘Estamino’ and ‘Maxifort,’ with the non-grafted determinant market tomatoes as a control. During the second year of this study, fertilizer treatment was introduced with two N rates (0% and 100%). At the end of the growing season, ripe tomatoes were harvested on a weekly basis, and yield was determined by weighing all tomatoes from the five plants in each experimental unit. Overall, there was no consistent improvement in total yield for any of the grafting treatments, and the estimated total mean yield of BHN-589 was at least 50% more than Nebraska Wedding. Moreover, there was no interaction effect between grafting and fertilizing treatment within each location. Results from this study suggest the need for more assessment on the effect of tomato grafting under different environmental conditions

Evaluation of Soilless Media Sensors for Managing Winter-time Greenhouse Strawberry Production using a CapMat System

It is important for a greenhouse strawberry grower to know that their capillary mat (CapMat™) fertigation system is working correctly and that plants are receiving the correct amounts of water and fertilizer. Pots with soilless mix are not expected to hold more than 70% water on a volumetric basis. Pots with less than 40% water content continuously are not supplied enough water and nutrients to the plants. Typically, pots located near the manifold distribution system get a little more water than those at the other locations, but water use will really vary according to the factors listed above as well as environmental parameters, but should not vary more than 20%. Fertigation is based on the drip tape distribution system, the media density of individual pots, and the spatial energy distribution within the greenhouse. To understand how these factors interact, pot moisture, media temperature, and electrical conductivity were spot checked with a relatively new commercial sensor and also monitored continuously along with greenhouse temperature, humidity, and photosynthetically active radiation (PAR) using a data logger system. We found that the variance in pot medium moisture and fertilizer was as expected as were fluctuations in air and mix temperatures. Calibrated commercial electrical conductivity and soil moisture sensors for measuring pot moisture and/or electric conductivity were reliable. Having this data may be a key to determining why plants in the UNL greenhouse produced more marketable fruit than plants in the commercial house

Strawberry cultivars vary in productivity, sugars and phytonutrient content when grown in a greenhouse during the winter

In many areas of the US, fresh locally grown berries are not available during the winter. With this in mind, a research study comprised of three experiments was conducted focused on cultivar selection for berry yield, number, sweetness and phytonutrient content. Using a capillary mat system with under bench heating within a double-layer polyethylene greenhouse, strawberries were grown in the Great Plains Region of the US during the winter. During experiment 1, 12 cultivars were grown; berries were weighed, counted and analyzed for sugars and phytonutrients. “Albion” plants produced a high number/mass of berries, had relatively high sugar content but a lower level of phytonutrients when compared to other cultivars. Sugar and phytonutrients concentrations overlapped across cultivars and thus, one cultivar could not be statistically singled out as best. As all cultivars flowered and fruited, two additional 8-month-long experiments were conducted. It took only 7 weeks from potting of dormant crowns for most cultivars to produce fruit. Certain cultivars fruited more successfully during certain months than others, but this was not associated with response time. For example,” “Albion”, “Chandler”, “Darselect”, “Evie-2” and “Seascape” plants consistently produced fruit October to early January while “AC Wendy”, “Cavendish”, “Honeoye” and “Strawberry Festival” plants mainly produced berries in March/April. Summed over experiment 2, “Albion”, “ Cavendish”, “Chandler”, “ Evie-2”, “Portola” and “Seascape” plants produced the greatest mass of berries. “AC Wendy” and “Darselect” berries contained some of the highest levels of sugars while berries from “Chandler”, “Darselect”, “Evie-2”, “Seascape” and “Strawberry Festival” had some of the highest phytonutrient values. In the third experiment, of the 8 selected cultivars, “Evie-2”, “Evie−2+” and “Portola” plants had the highest total yield and average berry mass/plant. “Seascape” and” Chandler” plants were second in total production. Glucose, fructose and sucrose levels varied across cultivars with “Chandler” and “Seascape” berries possessing the lowest level of total sugars. Phytonutrient values varied among cultivars with some having better flavonoids (“Seascape”), phenols (“Seascape” and “Chandler”) and ant oxidant capacity (“Seascape”, “Evie-2” and “Cavendish”). Measurement of soluble solids concentration varied by week among the cultivars with “Seascape”, “Seascape+”, and “Albion” berries possessing higher levels than other cultivars such as “Cavendish”. Overall, under these winter greenhouse conditions using capillary mat fertigation and an under-bench heat delivery system, strawberries were successfully produced for the off-season market

Comparison of Winter Strawberry Production in a Commercial Heated High Tunnel versus a University Greenhouse

For the past 4 years, the University of Nebraska strawberry team has worked to develop low cost, sustainable methods for farmers and growers to produce strawberries in a double polyethylene greenhouse during the winter. This past year, this growing system was adapted to become a commercial grower’s heated high tunnel for the winter/spring of 2013-14. The idea was to scale up to a farm-size demonstration and compare it to the university greenhouse production system with a goal to expand marketing opportunities for strawberries into the winter season

The electronic medication complete communication (EMC2) study: Rationale and methods for a randomized controlled trial of a strategy to promote medication safety in ambulatory care

AbstractBackgroundAdverse drug events (ADEs) affect millions of patients annually and place a significant burden on the healthcare system. The Food and Drug Administration (FDA) has developed patient safety information for high-risk medications that pose serious public health concerns. However, there are currently few assurances that patients receive this information or are able to identify or respond correctly to ADEs.ObjectiveTo evaluate the effectiveness of the Electronic Medication Complete Communication (EMC2) Strategy to promote safe medication use and reporting of ADEs in comparison to usual care.MethodsThe automated EMC2 Strategy consists of: 1) provider alerts to counsel patients on medication risks, 2) the delivery of patient-friendly medication information via the electronic health record, and 3) an automated telephone assessment to identify potential medication concerns or ADEs. The study will take place in two community health centers in Chicago, IL. Adult, English or Spanish-speaking patients (N=1200) who have been prescribed a high-risk medication will be enrolled and randomized to the intervention arm or usual care based upon practice location. The primary outcomes of the study are medication knowledge, proper medication use, and reporting of ADEs; these will be measured at baseline, 4weeks, and three months. Intervention fidelity as well as barriers and costs of implementation will be evaluated.ConclusionsThe EMC2 Strategy automates a patient-friendly risk communication and surveillance process to promote safe medication use while minimizing clinic burden. This trial seeks to evaluate the effectiveness and feasibility of this strategy in comparison to usual care

New Perspectives on Transforming States' Health and Human Services: Practical Commentaries on the First Year of the Work Support Strategies Initiative

Millions of low-income working families in America today are struggling to make ends meet. While working hard, often in low-wage jobs, many of these families are living close to the edge of hardship and have little or no resources to fall back on in case of emergencies. Public benefit programs can make a huge difference in the well-being of these working families, providing help with food, child care, and health insurance expenses. These programs help families address immediate needs and weather short-term crises, such as repairing a car needed to get to work or dealing with an unexpected health problem. They can make it possible for families to hold onto their jobs in these emergencies, stabilizing employment and keeping families from falling further into poverty. Yet many families that are eligible for public benefit programs do not participate. Although the recession and its aftermath led to unprecedented increases in receipt of nutrition assistance through the federal Supplemental Nutrition Assistance Program (SNAP), the latest data (from 2010) show that only 65 percent of the eligible working poor are participating. Similarly, of all children eligible for public health insurance coverage through Medicaid or the Children's Health Insurance Program, only 86 percent are participating. The participation rate for public health insurance for parents is only 66 percent. And, these participation rates vary widely across states.The Work Support Strategies, or WSS, Initiative is motivated by the value public benefit programs can provide to working families and the belief that the states and localities administering these programs can improve how eligible families access and retain these benefits. In the first year of the demonstration, nine states took on the challenge of streamlining, integrating, and improving the provision of work support benefits through their SNAP, Medicaid, and child care programs (and, in some states, additional programs such as heating assistance and cash welfare). While most states hope their efforts will also reduce burden on caseworkers and administrative costs in these systems, all are motivated to improve the lives of the families they serve

Identification of a Putative Salmonella enterica Serotype Typhimurium Host Range Factor with Homology to IpaH and YopM by Signature-Tagged Mutagenesis

The genetic basis for the host adaptation of Salmonella serotypes is currently unknown. We have explored a new strategy to identify Salmonella enterica serotype Typhimurium (S. typhimurium) genes involved in host adaptation, by comparing the virulence of 260 randomly generated signature-tagged mutants during the oral infection of mice and calves. This screen identified four mutants, which were defective for colonization of only one of the two host species tested. One mutant, which only displayed a colonization defect during the infection of mice, was further characterized. During competitive infection experiments performed with the S. typhimurium wild type, the mutant was defective for colonization of murine Peyer's patches but colonized bovine Peyer's patches at the wild-type level. No difference in virulence between wild type and mutant was observed when calves were infected orally with 10(10) CFU/animal. In contrast, the mutant possessed a sixfold increase in 50% lethal morbidity dose when mice were infected orally. The transposon in this mutant was inserted in a 2.9-kb pathogenicity islet, which is located between uvrB and yphK on the S. typhimurium chromosome. This pathogenicity islet contained a single gene, termed slrP, with homology to ipaH of Shigella flexneri and yopM of Yersinia pestis. These data show that comparative screening of signature-tagged mutants in two animal species can be used for scanning the S. typhimurium genome for genes involved in host adaptation

MYC regulates ribosome biogenesis and mitochondrial gene expression programs through its interaction with host cell factor-1.

The oncoprotein transcription factor MYC is a major driver of malignancy and a highly validated but challenging target for the development of anticancer therapies. Novel strategies to inhibit MYC may come from understanding the co-factors it uses to drive pro-tumorigenic gene expression programs, providing their role in MYC activity is understood. Here we interrogate how one MYC co-factor, host cell factor (HCF)-1, contributes to MYC activity in a human Burkitt lymphoma setting. We identify genes connected to mitochondrial function and ribosome biogenesis as direct MYC/HCF-1 targets and demonstrate how modulation of the MYC-HCF-1 interaction influences cell growth, metabolite profiles, global gene expression patterns, and tumor growth in vivo. This work defines HCF-1 as a critical MYC co-factor, places the MYC-HCF-1 interaction in biological context, and highlights HCF-1 as a focal point for development of novel anti-MYC therapies