The development of structural adhesive systems suitable for use with liquid oxygen Annual summary report, 1 Jul. 1964 - 30 Jun. 1965

Preparation and testing of adhesive polyurethanes, polycarbonates, and other highy halogenated polymers for liquid oxygen compatibilit

Resultados de la actividad de los Servicios de Información Telefónica sobre Teratógenos (SITTE y SITE) durante el año 2004 y análisis del nivel cultural de la población usuaria

Resultados de otras actividades del ECEMCWe present a summary of the activity of the two teratology information services: SITTE (for health professional) and SITE (for the general population), during 2004. A total of 6.407 calls were received, 1.456 of them were made by health professional and the remaining 4.951 by the general population. Drugs were, one more year, the most common question in either services, specially drugs affecting CNS. We also analyzed the cultural level of the women that used the SITE during the last ten years. The results show that most of the users of the service belong to the higher cultural levels, measured by the level of their educational studies. We conclude that is necessary to consider new strategies particularly aimed to reach to those women with low level of education (or low cultural level).N

La vacuna de la rubéola y el embarazo

TeratologíaRubella is a viral infection that when it occurs during pregnancy, especially during the first trimester, could cause congenital rubella embryophaty (CRE) with devastating consequences for the developing embryo and fetus. CRE includes serious birth defects such as: cataracts, deafness and heart disease, among others. However the frequency of CRE has decreased dramatically since the general use of rubella vaccine. The immigrant population in Spain constitutes a group of concern for rubella infection among other infectious diseases due to the lack of previous vaccination in many individuals. Thus, a vaccination programme towards immigrants, specially all young women should be considered. Pregnant women should not be vaccinated, and women who are not pregnant should avoid conceiving for at least 28 days following vaccination. However if a vaccination occurs during pregnancy, the potential risk is only theoretical and a tranquilizer counseling should be performed together with a high resolution ecography.N

Analysis of natural variants of the hepatitis C virus internal ribosome entry site reveals that primary sequence plays a key role in cap-independent translation

The HCV internal ribosome entry site (IRES) spans a region of ∼340 nt that encompasses most of the 5′ untranslated region (5′UTR) of the viral mRNA and the first 24–40 nt of the core-coding region. To investigate the implication of altering the primary sequence of the 5′UTR on IRES activity, naturally occurring variants of the 5′UTR were isolated from clinical samples and analyzed. The impact of the identified mutations on translation was evaluated in the context of RLuc/FLuc bicistronic RNAs. Results show that depending on their location within the RNA structure, these naturally occurring mutations cause a range of effects on IRES activity. However, mutations within subdomain IIId hinder HCV IRES-mediated translation. In an attempt to explain these data, the dynamic behavior of the subdomain IIId was analyzed by means of molecular dynamics (MD) simulations. Despite the loss of function, MD simulations predicted that mutant G266A/G268U possesses a structure similar to the wt-RNA. This prediction was validated by analyzing the secondary structure of the isolated IIId RNAs by circular dichroism spectroscopy in the presence or absence of Mg2+ ions. These data strongly suggest that the primary sequence of subdomain IIId plays a key role in HCV IRES-mediated translation

Molecular Basis of Ligand Dissociation in β-Adrenergic Receptors

The important and diverse biological functions of β-adrenergic receptors (βARs) have promoted the search for compounds to stimulate or inhibit their activity. In this regard, unraveling the molecular basis of ligand binding/unbinding events is essential to understand the pharmacological properties of these G protein-coupled receptors. In this study, we use the steered molecular dynamics simulation method to describe, in atomic detail, the unbinding process of two inverse agonists, which have been recently co-crystallized with β1 and β2ARs subtypes, along four different channels. Our results indicate that this type of compounds likely accesses the orthosteric binding site of βARs from the extracellular water environment. Importantly, reconstruction of forces and energies from the simulations of the dissociation process suggests, for the first time, the presence of secondary binding sites located in the extracellular loops 2 and 3 and transmembrane helix 7, where ligands are transiently retained by electrostatic and Van der Waals interactions. Comparison of the residues that form these new transient allosteric binding sites in both βARs subtypes reveals the importance of non-conserved electrostatic interactions as well as conserved aromatic contacts in the early steps of the binding process

Identification of NPR1-dependent and independent genes early induced by salicylic acid treatment in Arabidopsis

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Anales de Edafología y Agrobiología Tomo 22 Número 11-12

Mikromorphometrische untersuchungeli ·an hohlraumen im boden, van W. Kubiena, W. Beckmann und E. Geyger.-- Adsorción de amoníaco en los geles Si02-Mg0. Calores de adsorción, por Teófilo Fernández Alvarez.-- Los minerales de la arcilla en suelos de la provinc:ia de Salamanca, III, por J. L. Martín Vivaldi, M. Sánchez Camazano y F. Lucena Conde.-- Color en sedimentos, por J. Galván, C. Alvarez, J. Alonso y J. Catalán.-- Disolución parcial de sílice orgánica en suelos, por Josefina Benayas Casares.-- Clases de fósforo inorgánico cambiable isotópicamente en los suelos calizos, por P. de Arambarri.-- Los suelos de la cuenca media del río Tormes, por T. Martín Patiño, J. T. Alonso Pascual y F. Lucena Conde.-- Estudio térmico diferencial y termogravimétrico de sustancias húmicas, por J. A. Acle de Cáceres y M. Sánchez Camazano.-- Composición química de· la flor del limonero Verna. II. Fósforo, por A. Ortuño Martínez, F. Costa Y agüe y S. Llorente Franco.-- Composición química de la flor del limonero Verna. III. Potasio, por A. Ortuño Martínez, F. Costa Yagüe y S. Llorente Franco.-- Notas. Curso Internacional de Edafología y Biología Vegetal.-- Conferencias del Prof. Butenandt·y visitas a Centros del Consejo.-- Decenio Hidrológico Internacional.-- Nombramiento de Director de la Estación Experimental de León.-- Actividad de los Centros.-- Concesión de la Medalla de Oro de la Sociedad Nacional para la Protección de la Naturaleza.-- Curso de Tecnología CerámicaPeer reviewe