Developing Allometric Equations for Teak Plantations Located in the Coastal Region of Ecuador from Terrestrial Laser Scanning Data

Traditional studies aimed at developing allometric models to estimate dry above-ground biomass (AGB) and other tree-level variables, such as tree stem commercial volume (TSCV) or tree stem volume (TSV), usually involves cutting down the trees. Although this method has low uncertainty, it is quite costly and inefficient since it requires a very time-consuming field work. In order to assist in data collection and processing, remote sensing is allowing the application of non-destructive sampling methods such as that based on terrestrial laser scanning (TLS). In this work, TLS-derived point clouds were used to digitally reconstruct the tree stem of a set of teak trees (Tectona grandis Linn. F.) from 58 circular reference plots of 18 m radius belonging to three different plantations located in the Coastal Region of Ecuador. After manually selecting the appropriate trees from the entire sample, semi-automatic data processing was performed to provide measurements of TSCV and TSV, together with estimates of AGB values at tree level. These observed values were used to develop allometric models, based on diameter at breast height (DBH), total tree height (h), or the metric DBH2 × h, by applying a robust regression method to remove likely outliers. Results showed that the developed allometric models performed reasonably well, especially those based on the metric DBH2 × h, providing low bias estimates and relative RMSE values of 21.60% and 16.41% for TSCV and TSV, respectively. Allometric models only based on tree height were derived from replacing DBH by h in the expression DBH2 x h, according to adjusted expressions depending on DBH classes (ranges of DBH). This finding can facilitate the obtaining of variables such as AGB (carbon stock) and commercial volume of wood over teak plantations in the Coastal Region of Ecuador from only knowing the tree height, constituting a promising method to address large-scale teak plantations monitoring from the canopy height models derived from digital aerial stereophotogrammetry

One step protocol and green synthesis of 2-N,N-dimethylamino-3-alkyl(aryl)-2-oxido-1- hydro-2-benzo[1,3,2]diazaphosphinine-4-one derivatives

ABSTRACT. 2-N,N-dimethylamino-2-oxido-3-alkyl(aryl)-1-hydro-2-benzo[1,3,2]diazaphosphinin-4-one (2) derivatives  are synthesized by one pot reaction by reacting equimolar quantities of anthranilic acid, primary amine, and N,N-dimethylphosphoramic dichloride under reflux of ethanol with good yields. The structure of the compounds were established on the basis of their infrared, nuclear magnetic resonance spectral data (1H NMR, 13C NMR and 31P NMR), mass spectrometry, and elemental analysis.   KEY WORDS: Anthranilic acid, N,N-Dimethylphosphoramic dichloride, Diazaphosphinine Bull. Chem. Soc. Ethiop. 2022, 36(4), 859-864.                                                               DOI: https://dx.doi.org/10.4314/bcse.v36i4.11                                                   &nbsp

Expeditive synthesis of trithiotriazine-cored glycoclusters and inhibition of <i>Pseudomonas aeruginosa</i> biofilm formation

International audienceReadily accessible, low-valency glycoclusters based on a triazine core bearing D-galactose and L-fucose epitopes are able to inhibit biofilm formation by Pseudomonas aeruginosa. These multivalent ligands are simple to synthesize, are highly soluble, and can be either homofunctional or heterofunctional. The galactose-decorated cluster shows good affinity for Pseudomonas aeruginosa lectin lecA. They are convenient biological probes for investigating the roles of lecA and lecB in biofilm formation

Epimutations in both the TESK2 and MMACHC promoters in the Epi-cblC inherited disorder of intracellular metabolism of vitamin B12

Background: epi-cblC is a recently discovered inherited disorder of intracellular vitamin B12 metabolism associating hematological, neurological, and cardiometabolic outcomes. It is produced by an epimutation at the promoter common to CCDC163P and MMACHC, which results from an aberrant antisense transcription due to splicing mutations in the antisense PRDX1 gene neighboring MMACHC. We studied whether the aberrant transcription produced a second epimutation by encompassing the CpG island of the TESK2 gene neighboring CCDC163P. Methods: We unraveled the methylome architecture of the CCDC163P-MMACHC CpG island (CpG:33) and the TESK2 CpG island (CpG:51) of 17 epi-cblC cases. We performed an integrative analysis of the DNA methylome profiling, transcriptome reconstruction of RNA-sequencing (RNA-seq), chromatin immunoprecipitation sequencing (ChIP-Seq) of histone H3, and transcription expression of MMACHC and TESK2. Results: The PRDX1 splice mutations and activation of numerous cryptic splice sites produced antisense readthrough transcripts encompassing the bidirectional MMACHC/CCDC163P promoter and the TESK2 promoter, resulting in the silencing of both the MMACHC and TESK2 genes through the deposition of SETD2-dependent H3K36me3 marks and the generation of epimutations in the CpG islands of the two promoters. Conclusions: The antisense readthrough transcription of the mutated PRDX1 produces an epigenetic silencing of MMACHC and TESK2. We propose using the term 'epi-digenism' to define this epigenetic disorder that affects two genes. Epi-cblC is an entity that differs from cblC. Indeed, the PRDX1 and TESK2 altered expressions are observed in epi-cblC but not in cblC, suggesting further evaluating the potential consequences on cancer risk and spermatogenesis. Keywords: Epi-cblC; MMACHC; Methylmalonic aciduria and homocystinuria, cblC type; Promoter hypermethylation; Secondary epimutation; TESK2

Updated Big Bang Nucleosynthesis confronted to WMAP observations and to the Abundance of Light Elements

We improve Standard Big Bang Nucleosynthesis (SBBN) calculations taking into account new nuclear physics analyses (Descouvemont et al. 2003). Using a Monte-Carlo technique, we calculate the abundances of light nuclei versus the baryon to photon ratio.The results concerning omegab are compared to relevant astrophysical and cosmological observations. Consistency between WMAP, SBBN results and D/H data strengthens the deduced baryon density and has interesting consequences on cosmic chemical evolution. A significant discrepancy between the calculated Li-7 deduced from WMAP and the Spite plateau is clearly revealed. To explain this discrepancy three possibilities are invoked : uncertainties on the Li abundance, surface alteration of Li in the course of stellar evolution or poor knowledge of the reaction rates related to Be-7 destruction. In particular, the possible role of the up to now neglected Be-7(d,p)2He-4 and Be-7(d,alpha)Li5 reactions is considered. The impressive advances in CMB observations provide a strong motivation for more efforts in experimental nuclear physics and high quality spectroscopy to keep BBN in pace.Comment: accepted in ApJ, 22 pages, 5 figure

Cross-sections for nuclide production in 56Fe target irradiated by 300, 500,750, 1000, 1500, and 2600 MeV protons compared with data on hydrogen target irradiation by 300, 500, 750, 1000, and 1500 MeV/nucleon 56Fe ions

Cross-sections for radioactive nuclide production in 56Fe(p,x) reactions at 300, 500, 750, 1000, 1500, and 2600 MeV were measured using the ITEP U-10 proton accelerator. In total, 221 independent and cumulative yields of products of half-lives from 6.6 min to 312 days have been obtained via the direct-spectrometry method. The measured data have been compared with the experimental data obtained elsewhere by the direct and inverse kinematics methods and with calculations by 15 codes, namely: MCNPX (INCL, CEM2k, BERTINI, ISABEL), LAHET (BERTINI, ISABEL), CEM03 (.01, .G1, .S1), LAQGSM03 (.01, .G1, >.S1), CASCADE-2004, LAHETO, and BRIEFF. Most of our data are in a good agreement with the inverse kinematics results and disprove the results of some earlier activation measurements that were quite different from the inverse kinematics measurements. The most significant calculation-to-experiment differences are observed in the yields of the A<30 light nuclei, indicating that further improvements in nuclear reaction models are needed, and pointing out as well to a necessity of more complete measurements of such reactions.Comment: 53 pages, 9 figures, 6 tables, only pdf file, submitted to Phys. Rev.

Hepatoblast and mesenchymal cell-specific gene-expression in fetal rat liver and in cultured fetal rat liver cells

The aim of this study was to determine whether passaged rat fetal liver cells are functional hepatoblasts. Hepatocyte/hepatoblast- and liver myofibroblast-gene-expressions were studied in adult and fetal rat liver tissues as well as in primary and passaged cultures of isolated rat fetal liver cells at both the mRNA and protein level. Desmin- and Alpha-Smooth Muscle Actin (SMA)-positive cells were located in the walls of liver vessels, whereas Desmin-positive/SMA-negative cells were distributed within the liver parenchyma. Primary cultures contained Prox1-positive hepatoblasts, Desmin/SMA-positive myofibroblasts and only a few Desmin-positive/SMA-negative cells. Albumin and alpha-fetoprotein (AFP) could be detected in the primary cultures and to a lesser extent after the first passage. The number of Desmin-positive/SMA-negative cells decreased with successive passage, such that after the second passage, only Desmin/SMA-positive cells could be detected. SMA-gene-expression increased during the passages, suggesting that myofibroblasts become the major cell population of fetal liver cell cultures over time. This observation needs to be taken into account, should passaged fetal liver cells be used for liver cell transplantation. Moreover it contradicts the concept of epithelial-mesenchymal transformation and suggests rather that selective overgrowth of mesenchymal cells occurs in culture

The ocean sampling day consortium.

Ocean Sampling Day was initiated by the EU-funded Micro B3 (Marine Microbial Biodiversity, Bioinformatics, Biotechnology) project to obtain a snapshot of the marine microbial biodiversity and function of the world's oceans. It is a simultaneous global mega-sequencing campaign aiming to generate the largest standardized microbial data set in a single day. This will be achievable only through the coordinated efforts of an Ocean Sampling Day Consortium, supportive partnerships and networks between sites. This commentary outlines the establishment, function and aims of the Consortium and describes our vision for a sustainable study of marine microbial communities and their embedded functional traits