World Cup 2014 – professional training program for brazilian hotels

This paper presents an experience of implementing professional training program for the Brazilian hospitality industry in the 12 cities that will be the headquarters of the World Cup in 2014. This project was developed in the context of the program “Welcoming Cup”, of the Brazilian Ministry of Tourism, whose objective is to enable the tourism industry to attain international standards of quality in tourism services

Evaluation of mechanism involved in the analgesic effect of 15d-PGJ2 mediated by activation of receptors activated by proliferated perissome-gamma in macrophages in TMJ rats

Orientador: Juliana Trindade Clemente NapimogaDissertação (mestrado) - Universidade Estadual de Campinas, Faculdade de Odontologia de PiracicabaResumo: A 15-deoxy-?12,14-prostaglandinaJ2 (15d-PGJ2), um ligante natural dos receptores ativados por proliferadores de peroxissomas (PPARs), tem sido considerado uma promissora estratégia terapêutica para doenças inflamatórias. Estudos anteriores sugerem que o potencial efeito antinociceptivo e anti-inflamatório da 15d-PGJ2 na dor inflamatória da articulação temporomandibular (ATM) está vinculado a ativação de macrófagos residentes no tecido periarituclar. Sendo assim, o objetivo deste estudo foi avaliar: (1) se a 15d-PGJ2, ao se ligar receptor PPAR-?, promove alteração fenotípica nos macrófagos residentes tipo M1 para M2 via sinalização da hemoxigenase tipo 1 (OH-1) promovendo ação resolutiva do processo inflamatório; e (2) se a ação analgésica promovida pela liberação de opióides endógenos pelo macrófagos ativados pela 15d-PGJ2 é mediado ativação da via neuronal L-Arginina/NO/cGMP/K+ATP através da proteinoquinase dependente da Ca+2/Calmodulina na ATM de ratos. Para este estudo foram utilizados ratos machos Wistar (± 150g, n=8 animais por grupo), provenientes do CEMIB, previamente aprovados pela Comissão de Ética no Uso de Animais (#3414-1). Análises através do método ELISA demonstraram que a administração intra-articular de 15d-PGJ2 (100 ng/ATM) aumenta a liberação do fator de transcrição ativados do PPAR-? (P<0.05: One-way ANOVA, Teste de Tukey). O tratamento dos animais com o recrutador de macrófagos Tioglicolato (1%/ATM/dia) aumentou significativamente a liberação do fator de transcrição ativados do PPAR-? em animais tratados com 15d-PGJ2. A análise da expressão da proteinoquinase dependente de Ca+2/calmodulina não demonstrou diferença entre os grupos testados (P<0.05: One-way ANOVA, Tukey test). Os cortes histológicos dos tecidos periarticulares tratados com imunohistoquimica demonstraram positividade para os marcadores de macrófagos M1, M2 e OH-1. A marcação do M2 e OH-1 foi mais evidente nos animais tratados com tioglicolato + 15d-PGJ2. Os resultados sugerem que a administração periférica da 15d-PGJ2 na ATM de ratos ativa os receptores PPAR? localizados nos macrófagos residentes, induzindo alteração fenotípica para macrófagos tipo M2, através da via da heme oxigenase 1, promovendo resolução do processo inflamatório. O efeito antinociceptivo da 15d-PGJ2 é independente da ativação da proteinoquinase dependente de Ca+2/calmodulinaAbstract: The 15-deoxy-?12,14-prostaglandin J2 (15d-PGJ2), a natural ligand of receptors activated by peroxisome proliferators (PPARs), has been considered a promising therapeutic strategy for inflammatory diseases. Previous studies suggest that the potential antinociceptive and anti-inflammatory effect of 15d-PGJ2 inflammatory pain in the temporomandibular joint (TMJ) is linked to activation of resident macrophages in periarituclar tissue. Thus, the aim of this study was to evaluate: (1) to 15d-PGJ2, to bind PPAR? receptor, promotes phenotypic change in the resident type M1 macrophages to M2 via signaling hemoxigenase type 1 (OH-1) promoting action termination of the inflammatory process; and (2) whether the analgesic effect promoted by the release of endogenous opioids by macrophages activated by 15d-PGJ2 is mediated activation of neuronal pathway L-Arginine / NO / cGMP / K+ATP by protein kinase dependent Ca+2/ Calmodulin in ATM mice. For this study were used male Wistar rats (± 150 g, n = 8 animals per group), from the CEMIB previously approved by the Ethics Committee on Animal Use (# 3414-1). Analysis by ELISA showed that intra-articular administration of 15d-PGJ2 (100 ng / ATM) increases the release of the activated transcription factor PPAR? (P <0.05: two-way ANOVA, Tukey Test). The treatment of the animals with the recruiter thioglycolate macrophages (1%/ATM / day) significantly increased the release of activated transcription factor PPAR? in animals treated with 15d-PGJ2. The analysis of the expression of protein kinase dependent Ca+2/ calmodulin showed no difference between the groups tested (P <0.05: two-way ANOVA, Tukey test). Histological sections of the periarticular tissues treated with immunohistochemistry showed positivity for markers of macrophages M1, M2 and OH-1. The mark M2 and OH-1 was evident in animals treated with thioglycollate + 15d-PGJ2. The results suggest that peripheral administration of 15d-PGJ2 in the active ATM rat PPAR? receptors located in the resident macrophages, inducing phenotypic change to M2 type macrophage, through the pathway of heme oxygenase 1, promoting resolution of the inflammatory process. The antinociceptive effect of 15d-PGJ2 is independent of the activation of protein kinase dependent Ca+2/ calmodulinMestradoFisiologia OralMestre em OdontologiaCNPQCAPE

Selenium Fertilization in Tropical Pastures

Brazil is one of the largest meat producers. Meat along with other animal products have been responsible for its larger contribution as source of selenium (Se) for human. However, Se deficiency remains a concern because researches have indicated that this nutrient is found in low levels in Brazilian diet. Cattle in Brazil are fed basically from pasture, but there are strong evidences that soils contain low availability of Se; consequently plants and animals incorporate low Se levels. Pastures, Se fertilized, bring benefits to nutrition and health of animal consequently to humans already known in some countries. In contrast, Se fertilization on tropical weathered soils and tropical forages is little known. However, Se management as fertilizer in tropical environments requires researches involving field experiments, especially with animals, for establishing of safe and effective Se recommendations as fertilizer due to the Se toxicity potential and complexity in system of soil-plant-animal-human

A manual method to obtain platelet rich plasma

OBJECTIVE:This study is to report a manual method to obtain platelet rich plasma (PRP).METHODS:For this study 61 ml of peripheral blood was obtained and submitted to centrifugation at 541g for 5 min. The centrifugation separates the blood into three components: red blood cells, buffy coat and platelet rich plasma. Blood and platelet rich plasma samples were sent to the Hospital's Laboratory and platelets and leukocytes were measured.RESULTS:A sample of 637 blood donors was evaluated. The platelet yield efficiency was 86.77% and the increase in platelet concentration factor was 2.89 times. The increase in leukocyte concentration factor was 1.97 times.CONCLUSION:The method described here produces leukocyte-rich and platelet-rich plasma with a high platelet and leukocyte increased factor.Level of Evidence IV, Controlled Laboratory Study.Hospital do Coração Knee InstituteUniversidade Federal de São Paulo (UNIFESP) Escola Paulista de Medicina Department of Orthopedics and TraumatologyUNIFESP, EPM, Department of Orthopedics and TraumatologySciEL

RvE1 Impacts the Gingival Inflammatory Infiltrate by Inhibiting the T Cell Response in Experimental Periodontitis

Periodontitis is a chronic inflammatory disease associated with the formation of dysbiotic plaque biofilms and characterized by the progressive destruction of the alveolar bone. The transition from health to disease is characterized by a shift in periodontal immune cell composition, from mostly innate (neutrophils) to adaptive (T lymphocytes) immune responses. Resolvin E1 (RvE1) is a specialized pro-resolution mediator (SPMs), produced in response to inflammation, to enhance its resolution. Previous studies have indicated the therapeutic potential of RvE1 in periodontal disease; however, the impact of RvE1 in the microbial-elicited osteoclastogenic immune response remains uncharacterized in vivo. In the present study, we studied the impact of RvE1 on the gingival inflammatory infiltrate formation during periodontitis in a mouse model. First, we characterized the temporal-dependent changes of the main immune cells infiltrating the gingiva by flow cytometry. Then, we evaluated the impact of early or delayed RvE1 administration on the gingival immune infiltration and cervical lymph nodes composition. We observed a consistent inhibitory outcome on T cells -particularly effector T cells- and a protective effect on regulatory T cells (Tregs). Our data further demonstrated the wide range of actions of RvE1, its preventive role in the establishment of the adaptive immune response during inflammation, and bone protective capacity.publishedVersio

15d-PGJ(2)-loaded solid lipid nanoparticles: physicochemical characterization and evaluation of pharmacological effects on inflammation

FAPESP - FUNDAÇÃO DE AMPARO À PESQUISA DO ESTADO DE SÃO PAULOCNPQ - CONSELHO NACIONAL DE DESENVOLVIMENTO CIENTÍFICO E TECNOLÓGICO15-deoxy-Delta(12,14)-prostaglandin J(2) (15d-PGJ(2)), a peroxisome proliferator-activated receptor-gamma (PPAR-gamma) agonist, has physiological properties including pronounced anti-inflammatory activity, though it binds strongly to serum albumin. The use of solid lipid nanoparticles (SLN) can improve therapeutic properties increasing drug efficiency and availability. 15d-PGJ(2)-SLN was therefore developed and investigated in terms of its immunomodulatory potential. 15d-PGJ(2)-SLN and unloaded SLN were physicochemically characterized and experiments in vivo were performed. Animals were pretreated with 15d-PGJ(2)-SLN at concentrations of 3, 10 or 30 mu g.kg(-1) before inflammatory stimulus with carrageenan (Cg), lipopolysaccharide (LPS) or mBSA (immune response). Interleukins (IL-1 beta, IL-10 and IL-17) levels were also evaluated in exudates. The 15d-PGJ(2)-SLN system showed good colloidal parameters and encapsulation efficiency of 96%. The results showed that the formulation was stable for up to 120 days with low hemolytic effects. The 15d-PGJ(2)-SLN formulation was able to reduce neutrophil migration in three inflammation models tested using low concentrations of 15d-PGJ(2). Additionally, 15d-PGJ(2)-SLN increased IL-10 levels and reduced IL-1 beta as well as IL-17 in peritoneal fluid. The new 15d-PGJ(2)-SLN formulation highlights perspectives of a potent anti-inflammatory system using low concentrations of 15d-PGJ(2).15-deoxy-Delta(12,14)-prostaglandin J(2) (15d-PGJ(2)), a peroxisome proliferator-activated receptor-gamma (PPAR-gamma) agonist, has physiological properties including pronounced anti-inflammatory activity, though it binds strongly to serum albumin. The use of solid lipid nanoparticles (SLN) can improve therapeutic properties increasing drug efficiency and availability. 15d-PGJ(2)-SLN was therefore developed and investigated in terms of its immunomodulatory potential. 15d-PGJ(2)-SLN and unloaded SLN were physicochemically characterized and experiments in vivo were performed. Animals were pretreated with 15d-PGJ(2)-SLN at concentrations of 3, 10 or 30 mu g.kg(-1) before inflammatory stimulus with carrageenan (Cg), lipopolysaccharide (LPS) or mBSA (immune response). Interleukins (IL-1 beta, IL-10 and IL-17) levels were also evaluated in exudates. The 15d-PGJ(2)-SLN system showed good colloidal parameters and encapsulation efficiency of 96%. The results showed that the formulation was stable for up to 120 days with low hemolytic effects. The 15d-PGJ(2)-SLN formulation was able to reduce neutrophil migration in three inflammation models tested using low concentrations of 15d-PGJ(2). Additionally, 15d-PGJ(2)-SLN increased IL-10 levels and reduced IL-1 beta as well as IL-17 in peritoneal fluid. The new 15d-PGJ(2)-SLN formulation highlights perspectives of a potent anti-inflammatory system using low concentrations of 15d-PGJ(2)118e0161796FAPESP - FUNDAÇÃO DE AMPARO À PESQUISA DO ESTADO DE SÃO PAULOCNPQ - CONSELHO NACIONAL DE DESENVOLVIMENTO CIENTÍFICO E TECNOLÓGICOFAPESP - FUNDAÇÃO DE AMPARO À PESQUISA DO ESTADO DE SÃO PAULOCNPQ - CONSELHO NACIONAL DE DESENVOLVIMENTO CIENTÍFICO E TECNOLÓGICO2014/11016-8303555/2013-