5 research outputs found
Genetic Inactivation of European Sea Bass (Dicentrarchus labrax L.) Eggs Using UV-Irradiation: Observations and Perspectives
International audienceAndrogenesis is a form of uniparental reproduction leading to progenies inheriting only the paternal set of chromosomes. Ithas been achieved with variable success in a number of freshwater species and can be attained by artificial fertilization ofgenetically inactivated eggs following exposure to gamma (c), X-ray or UV irradiation (haploid androgenesis) and byrestoration of diploidy by suppression of mitosis using a pressure or thermal shock. The conditions for the geneticinactivation of the maternal genome in the European sea bass (Dicentrarchus labraxL.) were explored using differentcombinations of UV irradiation levels and durations. UV treatments significantly affected embryo survival and generated awide range of developmental abnormalities. Despite the wide range of UV doses tested (from 7.2 to 720 mJ.cm22), only onedose (60 mJ.cm22.min21with 1 min irradiation) resulted in a small percentage (14%) of haploid larvae at hatching in theinitial trials as verified by flow cytometry. Microsatellite marker analyses of three further batches of larvae produced by usingthis UV treatment showed a majority of larvae with variable levels of paternal and maternal contributions and only one larvadisplaying pure paternal inheritance. The results are discussed also in the context of an assessment of the UV-absorbancecharacteristics of egg extracts in this species that revealed the presence of gadusol, a compound structurally related tomycosporine-like amino acids (MAAs) with known UV-screening properties