Genomics and epidemiology for gastric adenocarcinomas (GE4GAC): a Brazilian initiative to study gastric cancer

Abstract Gastric cancer (GC) is the fifth most common type of cancer worldwide with high incidences in Asia, Central, and South American countries. This patchy distribution means that GC studies are neglected by large research centers from developed countries. The need for further understanding of this complex disease, including the local importance of epidemiological factors and the rich ancestral admixture found in Brazil, stimulated the implementation of the GE4GAC project. GE4GAC aims to embrace epidemiological, clinical, molecular and microbiological data from Brazilian controls and patients with malignant and pre-malignant gastric disease. In this letter, we summarize the main goals of the project, including subject and sample accrual and current findings

Rationale, study design, and analysis plan of the Alveolar Recruitment for ARDS Trial (ART): Study protocol for a randomized controlled trial

Background: Acute respiratory distress syndrome (ARDS) is associated with high in-hospital mortality. Alveolar recruitment followed by ventilation at optimal titrated PEEP may reduce ventilator-induced lung injury and improve oxygenation in patients with ARDS, but the effects on mortality and other clinical outcomes remain unknown. This article reports the rationale, study design, and analysis plan of the Alveolar Recruitment for ARDS Trial (ART). Methods/Design: ART is a pragmatic, multicenter, randomized (concealed), controlled trial, which aims to determine if maximum stepwise alveolar recruitment associated with PEEP titration is able to increase 28-day survival in patients with ARDS compared to conventional treatment (ARDSNet strategy). We will enroll adult patients with ARDS of less than 72 h duration. The intervention group will receive an alveolar recruitment maneuver, with stepwise increases of PEEP achieving 45 cmH(2)O and peak pressure of 60 cmH2O, followed by ventilation with optimal PEEP titrated according to the static compliance of the respiratory system. In the control group, mechanical ventilation will follow a conventional protocol (ARDSNet). In both groups, we will use controlled volume mode with low tidal volumes (4 to 6 mL/kg of predicted body weight) and targeting plateau pressure <= 30 cmH2O. The primary outcome is 28-day survival, and the secondary outcomes are: length of ICU stay; length of hospital stay; pneumothorax requiring chest tube during first 7 days; barotrauma during first 7 days; mechanical ventilation-free days from days 1 to 28; ICU, in-hospital, and 6-month survival. ART is an event-guided trial planned to last until 520 events (deaths within 28 days) are observed. These events allow detection of a hazard ratio of 0.75, with 90% power and two-tailed type I error of 5%. All analysis will follow the intention-to-treat principle. Discussion: If the ART strategy with maximum recruitment and PEEP titration improves 28-day survival, this will represent a notable advance to the care of ARDS patients. Conversely, if the ART strategy is similar or inferior to the current evidence-based strategy (ARDSNet), this should also change current practice as many institutions routinely employ recruitment maneuvers and set PEEP levels according to some titration method.Hospital do Coracao (HCor) as part of the Program 'Hospitais de Excelencia a Servico do SUS (PROADI-SUS)'Brazilian Ministry of Healt

Document management: analysis of collection of architectural maps

Este artigo é um estudo de caso do processo de digitalização das plantas de arquitetura do Centro de Artes e Comunicação da Universidade Federal de Pernambuco, realizado em parceria com o Memorial Denis Bernardes. Objetivando identificar a ocorrência da Gestão Eletrônica de Documentos - GED neste procedimento, tendo em vista a importância da garantia da integridade deste acervo e da possibilidade de sua consulta. A pesquisa foi realizada por meio de levantamento bibliográfico e observação in loco de todo o processo, que deu-se por meio da digitalização dos documentos, utilizando equipamento apropriado. Como resultado ficaram explicitadas as fragilidades do processo realizado ante os requisitos para adequada elaboração da GED, uma vez que o trabalho elaborado não contempla todas as etapas da Gestão Eletrônica de Documentos. Conclui-se a necessidade de adoção da GED, afim de salvaguardar a memória histórica e arquitetônica da Universidade Federal de Pernambuco ante seu importante papel social.SimThis article is a case study of the digitization process of the architecture plants of the Arts and Communication Center of the Federal University of Pernambuco, in partnership with the Denis Bernardes Memorial. Intending to identify the occurrence of Electronic Document Management - GED in this procedure, considering the importance of ensuring the integrity of this collection and the possibility of its consultation. The research was carried out by means of a bibliographical survey and in loco observation of the whole process, which was done through the digitization of the documents, using appropriate equipment. As a result, the weaknesses of the process carried out before the requirements for the proper elaboration of the GED were explained, since the work elaborated does not contemplate all the stages of Electronic Document Management. The need to adopt the GED was concluded in order to safeguard the historical and architectural memory of the Federal University of Pernambuco in view of its important social role.method. As a result, the weaknesses of the process carried out before the requirements for the proper elaboration of the EDM were made explicit

Towards a Blockchain-Based Secure Electronic Medical Record for Healthcare Applications

Electronic medical records (EMRs) are highly sensitive information shared among peers to keep up-to-date patient history. Providing security, privacy, and availability to these sensitive data is a challenge because, typically, after data publication the patient loses control over them. In this paper, we propose a blockchain-based approach to secure EMR for healthcare applications, where access control is patient-centric. Our proposal keeps encrypted EMRs in the blockchain, and the patient shares the decryption key only with healthcare professionals in which he/she trusts. Blockchain allows untrusted node, in a distributed peer-to-peer network to correctly and verifiably interact with each other, without any reliable intermediary. We investigate the scalability of our approach through simulations. Results show that it scales well since increasing the number of nodes in the network implies a linear increase in the size of the stored chain. Results also reveal that the time for inserting a new EMR in the blockchain remains low even when the number of nodes in the network increases

Concentrações de ANA e BAP na micropropagação de abacaxizeiro L. Merrill (Ananas comosus) e no cultivo hidropônico das plântulas obtidas in vitro The effect of ANA and BAP concentrations on the micropropagation and hydroponic cultures of pineapple

O efeito de diferentes concentrações de ANA e BAP na micropropagação do abacaxizeiro, bem como no cultivo em hidroponia das plântulas obtidas in vitro, foram estudados em brotos de abacaxizeiro da variedade Pérola, inoculados em meio de cultura básico MS, suplementado com os fitorreguladores BAP e ANA em diferentes concentrações. Caracteres morfológicos quantitativos relacionados ao crescimento dos brotos e das plântulas de abacaxizeiro foram avaliados respectivamente durante os cultivos in vitro e em hidroponia e mostraram que, o tratamento T1 (BAP = 1,0 mg L-1e ANA = 0,5 mg L-1) proporcionou a maior taxa média de regeneração de brotos e conseqüentemente uma maior produção de matéria fresca. Entretanto, a altura dos brotos e a formação de suas raízes foram maiores nos tratamentos T2 (BAP = 0,5 mg L-1 e ANA = 0,25 mg L-1 ) e T3 (BAP = 0,25 mg L-1 e ANA = 0,12 mg L-1 ). Após sessenta dias de cultivo em hidroponia, todas as plântulas oriundas do tratamento T1 apresentaram um bom desenvolvimento, expresso pela maioria dos caracteres morfológicos avaliados. O sistema de micropropagação utilizado neste trabalho possibilitou a obtenção de brotos de abacaxizeiro Pérola, em quantidade suficiente e ao mesmo tempo de fácil individualização, seguida da regeneração de plântulas que foram cultivadas em hidroponia.<br>The effect of different ANA and BAP concentrations on in vitro and hydroponic cultures were studied. Pineapple shoots derived from Pérola explants variety were inoculated in MS media containing BAP and ANA in different concentrations. Growth parameters of shoots and plantlets were measured for in vitro and hydroponic cultures. Showed a highest multiplication rates of shoots and consequently highest fresh matter production were obtained with BAP and ANA at the concentrations of 1,0 and 0,5 mg L-1 respectively. However, the shoot length as well as the root number formed were higher in the T2 (0,5 de BAP + 0,25 de ANA) and T3 (0,25 de BAP + 0,12 de ANA) treatments. The results showed that after sixty days of hydroponic culture and in the presence of T1 treatment, all plantlets had good developing that was observed in the majority of morphological growth parameters evaluated. The results showed that it is possible to obtain high quantities of shoots and then plantlets which could be cultivated in hydroponic culture using the pineapple micropropagation procedures methods