Compound Feed Technology in Sturgeon Fish Aquiculture

The modern fish processing industry of the Russian Federation sets the task of increasing the efficiency of the use and development of the resource potential of the fishery complex by means of aquaculture and mariculture development, including sturgeon fish breeding. In this case, complex processing through the rational use of by-products of fish and hydrobiont. In Russia, China, the EU and other countries, most fish processing enterprises do not rationally use by-products of fish cutting, which leads to violation of environmental safety (waste deposits are littered with waste from the fishing industry). The article discusses the prospects of using alternative plant materials in the diet of sturgeon fish, presents the recipes for full extruded compound feed for this group of organisms, the quality indicators of compound feed for sturgeon. A comprehensive assessment of the quality of compound feed products was obtained according to the physicochemical and structural indicators of compound feed. The optimal terms and conditions of storage of the obtained feed were found. Based on the performed tests, it was concluded that it is advisable to use raw materials of plant origin in the composition of compound feed, which contributes to the growth of the live weight of fish, leads to the replacement of the costly components of the compound feed with cheaper raw materials of plant origin, provided that the nutritional value is not reduced, and the period and conditions of storage meet the existing requirements

Features of Muscle Tissue Microstructure of Cattle in Industrial Agglomerations under the Environmental Pressure Conditions

The intensive development of the industrial sector, intensification of the agro-industrial complex, associated with the use of various fertilizers, active use of modern household chemicals lead to the constant increase in xenobiotics in the environment in both rural and urban agglomerations. There are settlements and farms within the industrial areas, therefore, the issue of accumulation of ecotoxicants in the organs and tissues of an animal, as well as the impact on the state of its health, is of particular importance. In the regions with tough environmental situation associated with anthropogenic contamination, xenobiotics of anthropogenic origin can directly or indirectly modify the activity of various body systems. The integral characteristic that reflects adaptive modifications of biota is the morphological and functional status of organs and tissues of an animal, including the state of muscle tissue. An analysis of the regenerative plastic potential of muscle tissue allows finding innovative approaches to assessing the effects of environmental impacts on animals. Up to the present day, the morphological and functional characteristics of muscle tissue in young animals and adult cattle have not been sufficiently studied in the conditions of the tough environmental situation of the Central Federal District of the Russian Federation. The issue of ecological pathologies of organs in productive animals is quite urgent for the territory of the Central Federal District with its developed agro-industrial complex and industry. The study of animals from the agglomeration of the large chemical plant showed that cattle react differently to pollutants. The animals demonstrated changes not only in hematological and biochemical parameters, but also in the morphological and functional status of muscle tissue

Mathematical model of plate heat exchanger for utilisation of waste heat from condensable gaseous streams

The mathematical model of vapour condensation from the mixture with noncondensing gas in Plate Heat Exchanger (PHE) channels is presented. The model accounts for the change of process parameters along the heat transfer surface and local features of heat and mass transfer processes in PHEs channels with plates of different corrugations geometry. It consists of the system of ordinary differential equations with considerably nonlinear right parts. The software for its solution by finite difference method is developed. The validity of the model is confirmed by comparison with the experiment for steam-air mixture condensation in a PHE channel sample

NUDT2 Disruption Elevates Diadenosine Tetraphosphate (Ap4A) and Down-Regulates Immune Response and Cancer Promotion Genes.

Regulation of gene expression is one of several roles proposed for the stress-induced nucleotide diadenosine tetraphosphate (Ap4A). We have examined this directly by a comparative RNA-Seq analysis of KBM-7 chronic myelogenous leukemia cells and KBM-7 cells in which the NUDT2 Ap4A hydrolase gene had been disrupted (NuKO cells), causing a 175-fold increase in intracellular Ap4A. 6,288 differentially expressed genes were identified with P < 0.05. Of these, 980 were up-regulated and 705 down-regulated in NuKO cells with a fold-change ≥ 2. Ingenuity® Pathway Analysis (IPA®) was used to assign these genes to known canonical pathways and functional networks. Pathways associated with interferon responses, pattern recognition receptors and inflammation scored highly in the down-regulated set of genes while functions associated with MHC class II antigens were prominent among the up-regulated genes, which otherwise showed little organization into major functional gene sets. Tryptophan catabolism was also strongly down-regulated as were numerous genes known to be involved in tumor promotion in other systems, with roles in the epithelial-mesenchymal transition, proliferation, invasion and metastasis. Conversely, some pro-apoptotic genes were up-regulated. Major upstream factors predicted by IPA® for gene down-regulation included NFκB, STAT1/2, IRF3/4 and SP1 but no major factors controlling gene up-regulation were identified. Potential mechanisms for gene regulation mediated by Ap4A and/or NUDT2 disruption include binding of Ap4A to the HINT1 co-repressor, autocrine activation of purinoceptors by Ap4A, chromatin remodeling, effects of NUDT2 loss on transcript stability, and inhibition of ATP-dependent regulatory factors such as protein kinases by Ap4A. Existing evidence favors the last of these as the most probable mechanism. Regardless, our results suggest that the NUDT2 protein could be a novel cancer chemotherapeutic target, with its inhibition potentially exerting strong anti-tumor effects via multiple pathways involving metastasis, invasion, immunosuppression and apoptosis

Data compression with ΣΠ\Sigma\Pi-approximations based on splines

summary:The paper contains short description of $\Sigma\Pi$-algorithm for the approximation of the function with two independent variables by the sum of products of one-dimensional functions. Some realizations of this algorithm based on the continuous and discrete splines are presented here. Few examples concerning with compression in the solving of approximation problems and colour image processing are described and discussed

Features of Muscle Tissue Microstructure of Cattle in Industrial Agglomerations Under the Environmental Pressure Conditions

The intensive development of the industrial sector, intensification of the agro-industrial complex, associated with the use of various fertilizers, active use of modern household chemicals lead to the constant increase in xenobiotics in the environment in both rural and urban agglomerations. There are settlements and farms within the industrial areas, therefore, the issue of accumulation of ecotoxicants in the organs and tissues of an animal, as well as the impact on the state of its health, is of particular importance. In the regions with tough environmental situation associated with anthropogenic contamination, xenobiotics of anthropogenic origin can directly or indirectly modify the activity of various body systems. The integral characteristic that reflects adaptive modifications of biota is the morphological and functional status of organs and tissues of an animal, including the state of muscle tissue. An analysis of the regenerative plastic potential of muscle tissue allows finding innovative approaches to assessing the effects of environmental impacts on animals. Up to the present day, the morphological and functional characteristics of muscle tissue in young animals and adult cattle have not been sufficiently studied in the conditions of the tough environmental situation of the Central Federal District of the Russian Federation. The issue of ecological pathologies of organs in productive animals is quite urgent for the territory of the Central Federal District with its developed agro-industrial complex and industry. The study of animals from the agglomeration of the large chemical plant showed that cattle react differently to pollutants. The animals demonstrated changes not only in hematological and biochemical parameters, but also in the morphological and functional status of muscle tissue

Experience of biotechnology method applications in meat processing industry

Nowadays one of the main regulatory documents governing the future of food production in Russia is the “Food Security Doctrine of the Russian Federation” formulated in order to implement the state economic policy in the field of ensuring food security of the Russian Federation, aimed at reliable provision of the country’s population with food products, the development of domestic agricultural and fishery complexes. In the past 40 years, the use of nano- and biotechnologies in the processing of animal and fish raw materials has been widely developed. A large number of scientific studies are aimed at biotechnology method applications in the production of meat products, in particular the use of enzyme proteolytic preparations. The aim of the research presented in this article was to study the dynamics of the hydrolysis of water-, saltand alkali-soluble fractions of low-grade beef and lamb proteins with Megaterin, an enzyme preparation of animal origin. The fulfilled studies allow more precise approach to the use of enzyme preparations for the processing of basic raw materials by the meat industry

Hemolytic Activity and Cytotoxicity of Synthetic Nanoclays with Montmorillonite Structure for Medical Applications

The factors influencing the appearance of toxicity in samples of synthetic montmorillonite with a systematically changing chemical composition Nax(Al, Mg)2-3Si4O10(OH)2 nH2O, which are potentially important for their use in medicine as drug carriers, targeted drug delivery systems, entero- and hemosorbents have been studied. Samples synthesized under hydrothermal conditions had the morphology of nanolayers self-organized into the nanosponge structures. The effect of the aluminum content, particle sizes, porosity, and ζ-potential of the samples on their toxicity was studied. The cytotoxic effect of the samples on eukaryotic cells Ea. hy 926 was determined using the MTT assay. The hemolytic activity of the samples in the wide concentration range in relation to human erythrocytes was also estimated. It has been established that the toxicity of aluminosilicate nanoparticles can be significantly reduced by correctly selecting their synthesis conditions and chemical composition, which opens up the opportunities for their use in medicine

Adsorption Properties and Hemolytic Activity of Porous Aluminosilicates in a Simulated Body Fluid

A study of the adsorption features of bovine serum albumin (BSA), sodium and potassium cations, and vitamin B1 by porous aluminosilicates with different structures in a medium simulating blood plasma was conducted. The objects of this study were synthetic silicates with a montmorillonite structure Na2x(Al2(1-x),Mg2x)Si4O10(OH)2·nH2O (x = 0.5, 0.9, 1), aluminosilicates of the kaolinite subgroup Al2Si2O5(OH)4 with different particle morphologies (spherical, nanosponge, nanotubular, and platy), as well as framed silicates (Beta zeolite). An assessment of the possibility of using aluminosilicates as hemosorbents for extracorporeal blood purification was carried out. For this purpose, the sorption capacity of the samples both with respect to model medium molecular weight toxicants (BSA) and natural blood components—vitamins and alkaline cations—was investigated. The samples were also studied by X-ray diffraction, electron microscopy, and low-temperature nitrogen adsorption. The zeta potential of the sample’s surfaces and the distribution of active centers on their surfaces by the method of adsorption of acid-base indicators were determined. A hemolytic test was used to determine the ability of the studied samples to damage the membranes of eukaryotic cells. Langmuir, Freundlich, and Temkin models were used to describe the experimental BSA adsorption isotherms. To process the kinetic data, pseudo-first-order and pseudo-second-order adsorption models were used. It was found that porous aluminosilicates have a high sorption capacity for medium molecular weight pathogens (up to 12 times that of activated charcoal for some samples) and low toxicity to blood cells. Based on the obtained results, conclusions were made about the prospects for the development of new selective non-toxic hemosorbents based on synthetic aluminosilicates with a given set of properties