Theory of extraordinary transmission of light through quasiperiodic arrays of subwavelength holes

By using a theoretical formalism able to work in both real and k-spaces, the physical origin of the phenomenon of extraordinary transmission of light through quasi-periodic arrays of holes is revealed. Long-range order present in a quasiperiodic array selects the wavevector(s) of the surface electromagnetic mode(s) that allows an efficient transmission of light through subwavelength holes.Comment: 4 pages, 4 figure

Properties of Deflagration Fronts and Models for Type Ia Supernovae

Detailed models of the explosion of a white dwarf, which include self-consistent calculations of the light curve and spectra, provide a link between observational quantities and the underlying explosion.These calculations assume spherical geometry and are based on parameterized descriptions of the burning front during the deflagration phase. Recently, first multi-dimensional calculations for nuclear burning fronts have been performed. Although a fully consistent treatment of the burning fronts is beyond the current state of the art, these calculations provided a new and better understanding of the physics, and new descriptions for the flame propagation have been proposed. Here, we have studied the influence on the results of previous analyses of Type Ia Supernovae, namely, the nucleosynthesis and structure of the expanding envelope. Our calculations are based on a set of delayed detonation models with parameters that give a good account of the optical and infrared light curves, and of the spectral evolution. In this scenario, the burning front propagates first in a deflagration mode and, subsequently, turns into a detonation. The explosions and light curves are calculated using a one-dimensional Lagrangian radiation-hydro code, including a detailed nuclear network.Comment: 9 pages, 4 figures, macros 'crckapb.sty'. The Astrophysical Journal (accepted

Type Ia supernovae and the ^{12}C+^{12}C reaction rate

The experimental determination of the cross-section of the ^{12}C+^{12}C reaction has never been made at astrophysically relevant energies (E<2 MeV). The profusion of resonances throughout the measured energy range has led to speculation that there is an unknown resonance at E\sim1.5 MeV possibly as strong as the one measured for the resonance at 2.14 MeV. We study the implications that such a resonance would have for the physics of SNIa, paying special attention to the phases that go from the crossing of the ignition curve to the dynamical event. We use one-dimensional hydrostatic and hydrodynamic codes to follow the evolution of accreting white dwarfs until they grow close to the Chandrasekhar mass and explode as SNIa. In our simulations, we account for a low-energy resonance by exploring the parameter space allowed by experimental data. A change in the ^{12}C+^{12}C rate similar to the one explored here would have profound consequences for the physical conditions in the SNIa explosion, namely the central density, neutronization, thermal profile, mass of the convective core, location of the runaway hot spot, or time elapsed since crossing the ignition curve. For instance, with the largest resonance strength we use, the time elapsed since crossing the ignition curve to the supernova event is shorter by a factor ten than for models using the standard rate of ^{12}C+^{12}C, and the runaway temperature is reduced from \sim8.14\times10^{8} K to \sim4.26\times10^{8} K. On the other hand, a resonance at 1.5 MeV, with a strength ten thousand times smaller than the one measured at 2.14 MeV, but with an {\alpha}/p yield ratio substantially different from 1 would have a sizeable impact on the degree of neutronization of matter during carbon simmering. We conclude that a robust understanding of the links between SNIa properties and their progenitors will not be attained until the ^{12}C+^{12}C reaction rate is measured at energies \sim1.5 MeV.Comment: 15 pages, 6 tables, 10 figures, accepted for Astronomy and Astrophysic

Mathematical Models for Estimating the Risk of vCJD Transmission

We present two different simple models for vCJD transmission by blood transfusion. Both models indicate that transfusions alone are unlikely to cause more than a few infections, unless the number of primary cases increases. To improve our models, future work should pursue data collection, empirical estimation of the model parameters, and examination of the underlying assumptions of our frameworks. Further improvements could also include examining susceptibility to vCJD infection by age group and iatrogenic infections introduced through surgical instruments. Regarding the latter, it may be worthwhile to conduct experiments to quantify the transmission of prions from an infected surgical instrument after repeated sterilization procedures

T-cell allorecognition of donor glutathione S-transferase T1 in plasma cell-rich rejection

AIM: To investigate the role of glutathione S-transferase T1 donor-specific T lymphocytes in plasma cell-rich rejection of liver allografts. METHODS: The study group included 22 liver transplant patients. Among them, 18 patients were mismatched for the glutathione S-transferase T1 (GSTT1) alleles (don+/rec-), and 4 were matched (don+/rec+). Seven of the mismatched patients produced anti-GSTT1 antibodies and developed plasma cell-rich rejection (former de novo immune hepatitis). For the detection of specific T lymphocytes, peripheral blood mononuclear cells were collected and stored in liquid nitrogen. The memory T cell response was studied by adding to the cell cultures to a mix of 39 custom-made, 15-mer overlapping peptides, which covered the entire GSTT1 amino acid sequence. The specific cellular response to peptides was analyzed by flow cytometry using the markers CD8, CD4, IL-4 and IFNγ. RESULTS: Activation of CD8+ T cells with different peptides was observed exclusively in the group of patients with plasma-cell rich rejection (3 out of 7), with production of IL-4 and/or IFNγ at a rate of 1%-4.92% depending on the peptides. The CD4+ response was most common and not exclusive for patients with the disease, where 5 out of 7 showed percentages of activated cells from 1.24% to 31.34%. Additionally, two patients without the disease but with the mismatch had cells that became stimulated with some peptides (1.45%-5.18%). Highly unexpected was the finding of a double positive CD4+CD8low T cell population that showed the highest degree of activation with some of the peptides in 7 patients with the mismatch, in 4 patients with plasma cell-rich rejection and in 3 patients without the disease. Unfortunately, CD4+CD8low cells represent 1% of the total number of lymphocytes, and stimulation could not be analyzed in 9 patients due to the low number of gated cells. Cells from the 4 patients included as controls did not show activation with any of the peptides. CONCLUSION: Patients with GSTT1 mismatch can develop a specific T-cell response, but the potential role of this response in the pathogenesis of plasma cell-rich rejection is unknown

Distinct ubiquitin binding modes exhibited by SH3 domains: Molecular determinants and functional implications

SH3 domains constitute a new type of ubiquitin-binding domains. We previously showed that the third SH3 domain (SH3-C) of CD2AP binds ubiquitin in an alternative orientation. We have determined the structure of the complex between first CD2AP SH3 domain and ubiquitin and performed a structural and mutational analysis to decipher the determinants of the SH3-C binding mode to ubiquitin. We found that the Phe-to-Tyr mutation in CD2AP and in the homologous CIN85 SH3-C domain does not abrogate ubiquitin binding, in contrast to previous hypothesis and our findings for the first two CD2AP SH3 domains. The similar alternative binding mode of the SH3-C domains of these related adaptor proteins is characterised by a higher affinity to C-terminal extended ubiquitin molecules. We conclude that CD2AP/CIN85 SH3-C domain interaction with ubiquitin constitutes a new ubiquitin-binding mode involved in a different cellular function and thus changes the previously established mechanism of EGF-dependent CD2AP/CIN85 mono-ubiquitination. © 2013 Ortega Roldan et al

Urinary Biomarkers for Kidney Disease in ATTR Amyloidosis

Aim: The detection and prognosis of nephropathy in transthyretin amyloidosis depends on albuminuria and renal function. Knowing that urinary levels of alpha-1 microglobulin and beta-2 microglobulin reflect tubular dysfunction while urinary alpha-2 macroglobulin implies glomerular damage, we decide investigate the diagnostic value of these markers in the patients with transthyretin amyloidosis. Methods: Serum and urinary samples collected from 30 patients and 11 asymptomatic carriers were tested for alpha-1 microglobulin, beta-2 microglobulin, alpha-2 macroglobulin, albumin, creatinine and cystatin C. Results: Pathological urinary alpha-1 microglobulin was detected in 17 patients, beta-2 microglobulin in 6 and alpha-2 macroglobulin in 5; 5 patients had albuminuria (mg/g creatinine) 30-300 and in 20 patients values >300 were present. Asymptomatic carriers did not present pathological excretion of these biomarkers and albuminuria was >30 in 1 individual. The excretion rates of alpha-1 microglobulin and beta-2 microglobulin were positively correlated with albuminuria (P<0.001), serum creatinine (P<0.05) and cystatin C (P<0.001). Urinary alpha-2 macroglobulin was almost exclusively found in the presence of albuminuria, although their levels do not correlate. Conclusion: Urinary biomarkers emerge as a potential approach to detect renal disease but unexpectedly, urinary alpha-2 macroglobulin was not a marker of the severity of albuminuria

Muerte de un lince ibérico. Establecimiento de la causa y la autoría

Las ciencias forenses se aplican desde hace poco tiempo como herramientas en las investigaciones de delitos contra el medio ambiente. En este trabajo se expone un caso pionero en cuanto a la utilización de estas técnicas en un caso de un delit o contra la fauna silvestre. La investigación se inicia tras la apari ción de un lince ibérico muerto en el interior de una finca, donde se encontraron también varios pollos supuestamente utilizados como cebos envenenados y otro cadáver, el de un zorro. El lince ib érico es uno de los mamíferos más amenazados del planeta. Se llevaron a cabo distintos análisis a partir de la necropsia de los cadáveres en el Centro de Análisis y Diagnóstico de la Fauna Silvestre (CAD), el laboratorio de referencia para la fauna silvestre pertene ciente a la Junta de Andalucía. Los resultados fuer on concluyentes, ambas muertes se produjeron como consecuencia de la ingestión de cebos envenenados con un plaguicida extremadamente tóxico, el aldicarb. También se encontró esta sustancia en los pollos recogidos en el interior de la finca. Con el fin de e stablecer la culpabilidad de los propietarios de la finca, que negaron tener nada que ver con las muertes y la colocación de los cebos, se utilizó una herramienta novedosa en este tipo de investigaciones, la genética forens e. En base a los resultados se co nsiguió establecer una relación de parentesco entre los pollos utilizados como cebos envenenados y los pollos propiedad reconocida de los sospechosos, que permitió demostrar que todos tenían el mismo origen. Gracias al trabajo conjunto y coordinado durante toda la investigación y el informe pericial elaborado por el laboratorio del CAD, la Consejería de Medio Ambiente y Organización del Territorio de la Junta de Andalucía consiguió una sentencia ejemplar contra los envenenador es.The application of forensic science as a research tool to resolve crimes against the environment is relatively rec ent. To our knowledge this is the first instance in which the forensic genetic have been used as a definitive evidence to find out guilty in crimes against the wildlife. Our research starts when an Iberian lynx was found dead into a farm; very close to sev eral chickens used as poisoned baits and a fox carcass. Iberian lynx is one of the most endangered mammals in the planet. A comprehensive investigation from the carcasses was performed in the Analysis and Diagnostic Center for Wildlife in Andalucia (CAD), the reference laboratory for wildlife of the Andalusian Government (Spain). The results determined that the deaths occurred as a result of ingestion o f baits poisoned with an extremely toxic pesticide, aldicarb. This substance was also found in chickens ga thered inside the farm. In order to establish the guilt of the owners of the farm, who refused to be related to the deaths and the placement of the b aits, an innovative tool was used in this kind of research, the forensic genetic. Based on the results we got, we established a relationship between the chickens used as poisoned baits and the chickens from the farmer, all of them had the same origin. Thanks to the coordinated actuation during the complete investigation and the official report by the CAD labor atory used in the trial, the Ministry of Environmental and Territorial Organization got an exemplary sentence against the poisoners

Bubble and Dew Point Calculations in Multicomponent and Multireactive Mixtures

Bubble and dew point calculations are useful in chemical engineering and play an important role in the study of separation equipments for non-reactive and reactive mixtures. To the best of the authors’s knowledge, few methods have been proposed for these calculations in systems with several chemical reactions. The objective of this paper is to introduce new conditions for performing bubble and dew point calculations in reactive mixtures. We have developed these conditions based on the application of transformed variables of Ung and Doherty (1995). Using these transformed variables, the solution space is restricted to compositions that are already at chemical equilibrium and by consequence the problem dimension is also reduced. The reliability and efficiency of three equation-solving methods are tested and compared using our equilibrium conditions: a) a simultaneous equation-solving approach using Newton method (SESN), b) an equation-decoupling approach using successive substitution method (EDSS) and c) an optimization approach using the stochastic optimization method Simulated Annealing(OSA). Our results indicated that even for simple reactive systems, bubble and dew point calculations are challenging for classical equation-solving methods and require robust strategies. We conclude that OSA and EDSS methods are reliable to locate bubble and dew points in reactive systems. EDSS is more efficient than OSA; however, OSA does not need initial guesses and is more suitable for difficult problems