Synthetic dye decolorization by three sources of fungal laccase

Decolorization of six synthetic dyes using three sources of fungal laccase with the origin of Aspergillus oryzae, Trametes versicolor, and Paraconiothyrium variabile was investigated. Among them, the enzyme from P. variabile was the most efficient which decolorized bromophenol blue (100%), commassie brilliant blue (91%), panseu-S (56%), Rimazol brilliant blue R (RBBR; 47%), Congo red (18.5%), and methylene blue (21.3%) after 3 h incubation in presence of hydroxybenzotriazole (HBT; 5 mM) as the laccase mediator. It was also observed that decolorization efficiency of all dyes was enhanced by increasing of HBT concentration from 0.1 mM to 5 mM. Laccase from A. oryzae was able to remove 53% of methylene blue and 26% of RBBR after 30 min incubation in absence of HBT, but the enzyme could not efficiently decolorize other dyes even in presence of 5 mM of HBT. In the case of laccase from T. versicolor, only RBBR was decolorized (93%) in absence of HBT after 3 h incubation. © 2012 Forootanfar et al.; licensee BioMed Central Ltd

Prevalence of smoking among the students resided at dormitories in Golestan university of medical sciences, Iran

Introduction: Cigarette smoking leads to harmful physical and emotional problems and also is a predisposed factor for the addiction. The aim of this study was an attempt to determine the rate of prevalence and causes of smoking among the students of Golestan University of Medical Sciences who resided in the dormitories. Material & Methods: A cross-sectional analytical study conducted among the resident students of dormitories in 2010. The sample size consisted of all the university dormitory students. The data gathered using a validated and reliable questionnaire. The data analyzed using SPSS software and statistical tests including Chi-square, Logistic regression and Independent t-test. Results: Of 669 students, 538 (80%) of them filled out the questionnaires completely. 67.3% (362 subjects) were female. 6.1% (33 students) were smoker and 83.5% of them had experiences of cigarette smoking. The most important reasons for the smoking tendency were “having a smoker friend in 33%, a personal interest 27% and as hobby in 24% of the cases”. There were significant relation between “age, sex, region and year of education” with smoking (P0.05). Conclusion: The results indicated low prevalence of cigarette smoking among the students. However, preventive measures should be taken to conduit youth toward healthier behaviors. It seems parental control and monitoring children’s friend finding are crucial issue. © 2014, Bangladesh Journal of Medical Science. All rights reserved

Diagnostic Accuracy of Portable Ultrasonography in Confirmation of Adequate Reduction of Distal Radius Fracture

Background: Distal radius fracture (DRF) is one of the most common injury presented to the emergency ward and compromise about one sixth of fractures managed by emergency physiciansMethods: This study was performed in ED of two regional hospitals in eastern part of Iran, with combined census of 150,000 patients per year, serving a largely low-income population.Results: Thirty patients were enrolled during the study period. Five patients required repeated M&R, one refuses to continue and 4 patients were reduced for the second time. Conclusion: In the present study we show that US-guided DRF reduction has high sensitivity and specificity to diagnosed reduction adequacy in comparison with standard radiography

Risk measurement in the global supply chain using monte-carlo simulation

Abstract Nowadays, logistics and supply chain management (SCM) is critical to compete in the current turbulent markets. In addition, in the global context, there are many uncertainties which affect on the market. One of the most important risks is supplier disruption. The first step to cope with these uncertainties is quantifying them. In this regard many researches have focused on the problem but measurement of the risk in the global SCM is yet a challenge. In the uncertain conditions, simulation is a good tool to study the system. This paper aims to study a global supply chain with related risks and measurement of the risks using simulation. Global aspects considered in the paper are: 1-currency exchange rate, 2-extended leadtime for abroad supplies, 3-regional and local uncertainties. In this regard, two popular risk measurement approaches (VaR and CVaR) are used in the simulation of uncertainties in the global supply chain. Results showed that adopting risk averse behavior to cope with the uncertainties leads to the lower stockouts and also higher costs

Association between 318C/T polymorphism of the CTLA-4 gene and systemic lupus erythematosus in Iranian patients

Background: Cytotoxic T lymphocyte-associated antigen-4 (CTLA-4) is an important negative regulator of T-cell response. It is a functional candidate gene connected with susceptibility to systemic lupus erythematosus (SLE). We analyzed the role of -318C/T polymorphism in the promoter region of the CTLA-4 gene in Iranian patients suffering from SLE. Methods: A total of 180 SLE patients and 304 healthy ethnically matched controls were enrolled in the study. DNA was extracted from blood samples according to the standard procedure. Polymerase chain reaction restriction fragments length polymorphism (PCR-RFLP) was used to analyze the genotype and allele frequencies of these polymorphisms. Results: The CC genotype was observed in 170 (94.5%) of the SLE patients, which was significantly different compared to the controls (251 [82.4%]; P = 0.0001, OR = 3.51 95%CI = 1.77-7.53). T allele was significantly more common in the controls (9.2%) compared to SLE patients 2.8% (P = 0.0001, OR = 0.26, 95%CI = 0.13-0.53). There was no significant correlation between different genotypes and age, gender or family history of SLE in the studied population. Conclusion: It can be concluded that -318C/T polymorphism of CTLA-4 gene might play a significant role in the development of SLE in the Iranian patients. © 2014 Asia Pacific League of Associations for Rheumatology and Wiley Publishing Asia Pty Ltd

FPGA-based Assessment of Midori and GIFT Lightweight Block Ciphers

Lightweight block ciphers are today of paramount importance to provide security services in constrained environments. Recent studies have questioned the security properties of PRESENT, which makes it evident the need to study alternative ciphers. In this work we provide hardware architectures for Midori and GIFT, and compare them against implementations for PRESENT and GIMLI under fair conditions. The hardware description for our designs is made publicly available

The CanOE Strategy: Integrating Genomic and Metabolic Contexts across Multiple Prokaryote Genomes to Find Candidate Genes for Orphan Enzymes

Of all biochemically characterized metabolic reactions formalized by the IUBMB, over one out of four have yet to be associated with a nucleic or protein sequence, i.e. are sequence-orphan enzymatic activities. Few bioinformatics annotation tools are able to propose candidate genes for such activities by exploiting context-dependent rather than sequence-dependent data, and none are readily accessible and propose result integration across multiple genomes. Here, we present CanOE (Candidate genes for Orphan Enzymes), a four-step bioinformatics strategy that proposes ranked candidate genes for sequence-orphan enzymatic activities (or orphan enzymes for short). The first step locates “genomic metabolons”, i.e. groups of co-localized genes coding proteins catalyzing reactions linked by shared metabolites, in one genome at a time. These metabolons can be particularly helpful for aiding bioanalysts to visualize relevant metabolic data. In the second step, they are used to generate candidate associations between un-annotated genes and gene-less reactions. The third step integrates these gene-reaction associations over several genomes using gene families, and summarizes the strength of family-reaction associations by several scores. In the final step, these scores are used to rank members of gene families which are proposed for metabolic reactions. These associations are of particular interest when the metabolic reaction is a sequence-orphan enzymatic activity. Our strategy found over 60,000 genomic metabolons in more than 1,000 prokaryote organisms from the MicroScope platform, generating candidate genes for many metabolic reactions, of which more than 70 distinct orphan reactions. A computational validation of the approach is discussed. Finally, we present a case study on the anaerobic allantoin degradation pathway in Escherichia coli K-12

Effect of primary care physicians' use of estimated glomerular filtration rate on the timing of their subspecialty referral decisions

<p>Abstract</p> <p>Background</p> <p>Primary care providers' suboptimal recognition of the severity of chronic kidney disease (CKD) may contribute to untimely referrals of patients with CKD to subspecialty care. It is unknown whether U.S. primary care physicians' use of estimated glomerular filtration rate (eGFR) rather than serum creatinine to estimate CKD severity could improve the timeliness of their subspecialty referral decisions.</p> <p>Methods</p> <p>We conducted a cross-sectional study of 154 United States primary care physicians to assess the effect of use of eGFR (versus creatinine) on the timing of their subspecialty referrals. Primary care physicians completed a questionnaire featuring questions regarding a hypothetical White or African American patient with progressing CKD. We asked primary care physicians to identify the serum creatinine and eGFR levels at which they would recommend patients like the hypothetical patient be referred for subspecialty evaluation. We assessed significant improvement in the timing [from eGFR < 30 to ≥ 30 mL/min/1.73m²) of their recommended referrals based on their use of creatinine versus eGFR.</p> <p>Results</p> <p>Primary care physicians recommended subspecialty referrals later (CKD more advanced) when using creatinine versus eGFR to assess kidney function [median eGFR 32 versus 55 mL/min/1.73m², p < 0.001]. Forty percent of primary care physicians significantly improved the timing of their referrals when basing their recommendations on eGFR. Improved timing occurred more frequently among primary care physicians practicing in academic (versus non-academic) practices or presented with White (versus African American) hypothetical patients [adjusted percentage(95% CI): 70% (45-87) versus 37% (reference) and 57% (39-73) versus 25% (reference), respectively, both p ≤ 0.01).</p> <p>Conclusions</p> <p>Primary care physicians recommended subspecialty referrals earlier when using eGFR (versus creatinine) to assess kidney function. Enhanced use of eGFR by primary care physicians' could lead to more timely subspecialty care and improved clinical outcomes for patients with CKD.</p