Validation Through Simulations of a Cn2 Profiler for the ESO/VLT Adaptive Optics Facility

The Adaptive Optics Facility (AOF) project envisages transforming one of the VLT units into an adaptive telescope and providing its ESO (European Southern Observatory) second generation instruments with turbulence corrected wavefronts. For MUSE and HAWK-I this correction will be achieved through the GALACSI and GRAAL AO modules working in conjunction with a 1170 actuators Deformable Secondary Mirror (DSM) and the new Laser Guide Star Facility (4LGSF). Multiple wavefront sensors will enable GLAO and LTAO capabilities, whose performance can greatly benefit from a knowledge about the stratification of the turbulence in the atmosphere. This work, totally based on end-to-end simulations, describes the validation tests conducted on a Cn2 profiler adapted for the AOF specifications. Because an absolute profile calibration is strongly dependent on a reliable knowledge of turbulence parameters r0 and L0, the tests presented here refer only to normalized output profiles. Uncertainties in the input parameters inherent to the code are tested as well as the profiler response to different turbulence distributions. It adopts a correction for the unseen turbulence, critical for the GRAAL mode, and highlights the effects of masking out parts of the corrected wavefront on the results. Simulations of data with typical turbulence profiles from Paranal were input to the profiler, showing that it is possible to identify reliably the input features for all the AOF modes.Comment: 15 pages, 12 figures, accepted for publication in the MNRAS Accepted 2015 January 22. Received 2015 January 21; in original form 2014 December

Physiological traits of Penicillium glabrum strain LCP 08.5568, a filamentous fungus isolated from bottled aromatised mineral water

International audiencePenicillium glabrum is an ubiquitous fungus distributed world wide. This fungus is a frequent contaminant in the food manufacturing industry. Environmental factors such as temperature, water activity and pH have a great influence on fungal development. In this study, a strain of P. glabrum referenced to as LCP 08.5568, has been isolated from a bottle of aromatised mineral water. The effects of temperature, aw and pH on radial growth rate were assessed on Czapeck Yeast Agar (CYA) medium. Models derived from the cardinal model with inflection (Rosso et al., 1993 An unexpected correlation between cardinal temperatures of microbial growth highlighted by a new model. J Theor. Bio. 162, 447-463) were used to fit the experimental data and determine for each factor, the cardinal parameters (minimum, optimum and maximum). Precise characterisation of the growth conditions for such a fungal contaminant, has an evident interest to understand and to prevent spoilage of food products

Calibration of quasi-static aberrations in exoplanet direct-imaging instruments with a Zernike phase-mask sensor. II. Concept validation with ZELDA on VLT/SPHERE

Warm or massive gas giant planets, brown dwarfs, and debris disks around nearby stars are now routinely observed by dedicated high-contrast imaging instruments on large, ground-based observatories. These facilities include extreme adaptive optics (ExAO) and state-of-the-art coronagraphy to achieve unprecedented sensitivities for exoplanet detection and spectral characterization. However, differential aberrations between the ExAO sensing path and the science path represent a critical limitation for the detection of giant planets with a contrast lower than a few $10^{-6}$ at very small separations (<0.3\as) from their host star. In our previous work, we proposed a wavefront sensor based on Zernike phase contrast methods to circumvent this issue and measure these quasi-static aberrations at a nanometric level. We present the design, manufacturing and testing of ZELDA, a prototype that was installed on VLT/SPHERE during its reintegration in Chile. Using the internal light source of the instrument, we performed measurements in the presence of Zernike or Fourier modes introduced with the deformable mirror. Our experimental and simulation results are consistent, confirming the ability of our sensor to measure small aberrations (<50 nm rms) with nanometric accuracy. We then corrected the long-lived non-common path aberrations in SPHERE based on ZELDA measurements. We estimated a contrast gain of 10 in the coronagraphic image at 0.2\as, reaching the raw contrast limit set by the coronagraph in the instrument. The simplicity of the design and its phase reconstruction algorithm makes ZELDA an excellent candidate for the on-line measurements of quasi-static aberrations during the observations. The implementation of a ZELDA-based sensing path on the current and future facilities (ELTs, future space missions) could ease the observation of the cold gaseous or massive rocky planets around nearby stars.Comment: 13 pages, 12 figures, A&A accepted on June 3rd, 2016. v2 after language editin

Smart Focal Plane Technologies for VLT Instruments

As we move towards the era of ELTs, it is timely to think about the future role of the 8-m class telescopes. Under the OPTICON programme, novel technologies have been developed that are intended for use in multi-object and integral-field spectrographs. To date, these have been targeted at instrument concepts for the European ELT, but there are also significant possibilities for their inclusion in new VLT instruments, ensuring the continued success and productivity of these unique telescopes.Comment: 5 pages, to appear in the proceedings of the ESO Workshop "Science with the VLT in the ELT era

Variational assimilation of Lagrangian data in oceanography

We consider the assimilation of Lagrangian data into a primitive equations circulation model of the ocean at basin scale. The Lagrangian data are positions of floats drifting at fixed depth. We aim at reconstructing the four-dimensional space-time circulation of the ocean. This problem is solved using the four-dimensional variational technique and the adjoint method. In this problem the control vector is chosen as being the initial state of the dynamical system. The observed variables, namely the positions of the floats, are expressed as a function of the control vector via a nonlinear observation operator. This method has been implemented and has the ability to reconstruct the main patterns of the oceanic circulation. Moreover it is very robust with respect to increase of time-sampling period of observations. We have run many twin experiments in order to analyze the sensitivity of our method to the number of floats, the time-sampling period and the vertical drift level. We compare also the performances of the Lagrangian method to that of the classical Eulerian one. Finally we study the impact of errors on observations.Comment: 31 page

Deciphering the infectious process of Colletotrichum lupini in lupin through transcriptomic and proteomic analysis

The fungal phytopathogen Colletotrichum lupini is responsible for lupin anthracnose, resulting in significant yield losses worldwide. The molecular mechanisms underlying this infectious process are yet to be elucidated. This study proposes to evaluate C. lupini gene expression and protein synthesis during lupin infection, using, respectively, an RNAseq-based transcriptomic approach and a mass spectrometry-based proteomic approach. Patterns of differentially-expressed genes in planta were evaluated from 24 to 84 hours post-inoculation, and compared to in vitro cultures. A total of 897 differentially-expressed genes were identified from C. lupini during interaction with white lupin, of which 520 genes were predicted to have a putative function, including carbohydrate active enzyme, effector, protease or transporter-encoding genes, commonly described as pathogenicity factors for other Colletotrichum species during plant infection, and 377 hypothetical proteins. Simultaneously, a total of 304 proteins produced during the interaction were identified and quantified by mass spectrometry. Taken together, the results highlight that the dynamics of symptoms, gene expression and protein synthesis shared similarities to those of hemibiotrophic pathogens. In addition, a few genes with unknown or poorly-described functions were found to be specifically associated with the early or late stages of infection, suggesting that they may be of importance for pathogenicity. This study, conducted for the first time on a species belonging to the Colletotrichum acutatum species complex, presents an opportunity to deepen functional analyses of the genes involved in the pathogenicity of Colletotrichum spp. during the onset of plant infection

A narrow, edge-on disk resolved around HD 106906 with SPHERE

HD~106906AB is so far the only young binary system around which a planet has been imaged and a debris disk evidenced thanks to a strong IR excess. As such, it represents a unique opportunity to study the dynamics of young planetary systems. We aim at further investigating the close (tens of au scales) environment of the HD~106906AB system. We used the extreme AO fed, high contrast imager SPHERE recently installed on the VLT to observe HD~106906. Both the IRDIS imager and the Integral Field Spectrometer were used. We discovered a very inclined, ring-like disk at a distance of 65~au from the star. The disk shows a strong brightness asymmetry with respect to its semi-major axis. It shows a smooth outer edge, compatible with ejection of small grains by the stellar radiation pressure. We show furthermore that the planet's projected position is significantly above the disk's PA. Given the determined disk inclination, it is not excluded though that the planet could still orbit within the disk plane if at a large separation (2000--3000 au). We identified several additional point sources in the SPHERE/IRDIS field-of-view, that appear to be background objects. We compare this system with other debris disks sharing similarities, and we briefly discuss the present results in the framework of dynamical evolution.Comment: 7 pages, 6 figures, accepted by Astronomy & Astrophysic

Precision measurement of the half-life and the decay branches of 62Ga

In an experiment performed at the Accelerator Laboratory of the University of Jyvaskyla, the beta-decay half-life of 62Ga has been studied with high precision using the IGISOL technique. A half-life of T1/2 = 116.09(17)ms was measured. Using beta-gamma coincidences, the gamma intensity of the 954keV transition and an upper limit of the beta-decay feeding of the 0+_2 state have been extracted. The present experimental results are compared to previous measurements and their impact on our understanding of the weak interaction is discussed.Comment: 7 pages, 7 figures, submitted to EPJ

Detection of Yersinia enterocolitica on slaughtered pig tonsils in France

This study was conducted to obtain data about the presence of Yersinia enterocolitica (YE) in French slaughtered pigs and to evaluate detection methods. Nine hundred tonsil swabs were taken from pigs at slaughter (one slaughterhouse in Brittany, France, 45 batches of pigs with 20 pigs sampled per batch from January to March 2009). The swabs were vortexed in 10 ml PSB broth and I ml was transferred in 9 ml lTC broth. After 48h at 25°C, PSB enrichment was streaked on CIN plates and lTC enrichment on SSDC and CIN plates