Viewpoint (Art and Descriptive Text)

This art was created as part of a class zine project. The concept/direction for the zine was generated by a group of students, then selected by the class for use. This cover was a collaborative interpretation of that concept conceived of by Kaylyn Hill, Stacey Roten, Siyu Wang, and Jimmy Wilder. The design was executed by Kaylyn Hill. The problem the class addressed was: Imagine that hundreds of designers used their skills and creativity to respond to the most important and newsworthy issues of today. This collection will be the focus of a blog, a book, an exhibition, a documentary... (it doesn’t really matter what format or media it might be, ultimately). How would this kind of graphic statement—this power used for good, for awareness, for protest, etc.—be expressed? What would you title it? What visual treatment might be applied to or accompany that title? The Viewpoint #nofilter concept reflects the act of looking closely and critically at world events and responding with honesty. Therefore, there are many perspectives of the world shown but within a form that also resembles an eye, a target, a print registration mark, etc. The art was modified for the cover of PURE Insights, but is presented here in the original form

Describing ancient horizontal gene transfers at the nucleotide and gene levels by comparative pathogenicity island genometrics

Motivation: Lateral gene transfer is a major mechanism contributing to bacterial genome dynamics and pathovar emergence via pathogenicity island (PAI) spreading. However, since few of these genomic exchanges are experimentally reproducible, it is difficult to establish evolutionary scenarios for the successive PAI transmissions between bacterial genera. Methods initially developed at the gene and/or nucleotide level for genomics, i.e. comparisons of concatenated sequences, ortholog frequency, gene order or dinucleotide usage, were combined and applied here to homologous PAIs: we call this approach comparative PAI genometrics. Results: YAPI, a Yersinia PAI, and related islands were compared with measure evolutionary relationships between related modules. Through use of our genometric approach designed for tracking codon usage adaptation and gene phylogeny, an ancient inter-genus PAI transfer was oriented for the first time by characterizing the genomic environment in which the ancestral island emerged and its subsequent transfers to other bacterial genera. Contact: [email protected] Supplementary informatio

Minigap in a long disordered SNS junction: analytical results

We review and refine analytical results on the density of states in a long disordered superconductor - normal-metal - superconductor junction with transparent interfaces. Our analysis includes the behavior of the minigap near phase differences zero and PI across the junction, as well as the density of states at energies much larger than the minigap but much smaller than the superconducting gap.Comment: 4 page

Preliminary investigation of Phase Doppler derived flux measurements in a wind tunnel for the sampling of orchard spray drift

Air-assisted spray equipment used for horticultural cropping systems depend on high air velocities to project the spray as well as to open the canopy for greater droplet penetration and deposition. However, these sprayer-types are also at a heightened risk for spray drift as they possess the potential to place drift prone droplets in the atmosphere where they can be carried to off-target locations. Unfortunately, quantifying these droplets can be difficult and expensive using samplers such as high-volume air samplers, rotating rods and strings. However, while these measuring techniques may give some idea of flux, no particle information can be gained which is imperative to predicting the mass which may be the most prone to drift. In wind-tunnels and field studies, polyester and nylon strings have proven to be an efficient collecting surface. Therefore, it was the objective of this study to assess the potential for the use of a novel, field grade Phase Doppler Interferometer (PDI) as a replacement for strings as a sampler for driftable mass for orchard type sprayers

Molar substitution and C2/C6 ratio of hydroxyethyl starch: influence on blood coagulation

Background. Development of hydroxyethyl starches (HES) with a low impact on blood coagulation but a long intravascular persistence is of clinical interest. A previous in vitro study showed that low substituted high molecular weight HES does not compromise blood coagulation more than medium molecular weight HES. In the present study we assessed the individual effects on blood coagulation of molar substitution and C2/C6 ratio of a high molecular weight HES. Methods. Blood was obtained from 30 healthy patients undergoing elective surgery and mixed with six high molecular weight (700 kDa) HES solutions differing in their molar substitution (0.42 and 0.51) and C2/C6 ratio (2.7, 7 and 14) to achieve 20, 40 and 60% dilution. Blood coagulation was assessed by Thrombelastograph® analysis (TEG) and plasma coagulation tests.Data were compared using a three-way analysis of variance model with repeated measures on the three factors. Results. Higher molar substitution compromised blood coagulation most (for all TEG parameters, P0.50). The higher molar substitution was associated with a lesser increase in PT (P=0.007) and a greater decrease in factor VIII (P=0.010). PTT, functional and antigenic von Willebrand factors were not significantly influenced by molar substitution (P for all >0.20). No significant differences between solutions with the same molar substitution but different C2/C6 ratios were found in plasma coagulation parameters (P for all >0.05). Conclusions. TEG analysis indicates that high molecular HES with a molar substitution of 0.42 and a C2/C6 ratio of 2.7 has the lowest effect on in vitro human blood coagulatio

Scientists and the 3Rs: attitudes to animal use in biomedical research and the effect of mandatory training in laboratory animal science

The 3Rs principle of replacement, reduction, and refinement has increasingly been endorsed by legislators and regulatory bodies as the best approach to tackle the ethical dilemma presented by animal experimentation in which the potential benefits for humans stand against the costs borne by the animals. Even when animal use is tightly regulated and supervised, the individual researcher’s responsibility is still decisive in the implementation of the 3Rs. Training in laboratory animal science (LAS) aims to raise researchers’ awareness and increase their knowledge, but its effect on scientists’ attitudes and practice has not so far been systematically assessed. Participants (n=206) in eight LAS courses (following the Federation of European Laboratory Animal Science Associations category C recommendations) in Portugal were surveyed in a self-administered questionnaire during the course. Questions were related mainly to the 3Rs and their application, attitudes to animal use and the ethical review of animal experiments. One year later, all the respondents were asked to answer a similar questionnaire (57% response rate) with added self-evaluation questions on the impact of training. Our results suggest that the course is effective in promoting awareness and increasing knowledge of the 3Rs, particularly with regard to refinement. However, participation in the course did not change perceptions on the current and future needs for animal use in research

Can Life develop in the expanded habitable zones around Red Giant Stars?

We present some new ideas about the possibility of life developing around sub-giant and red giant stars. Our study concerns the temporal evolution of the habitable zone. The distance between the star and the habitable zone, as well as its width, increases with time as a consequence of stellar evolution. The habitable zone moves outward after the star leaves the main sequence, sweeping a wider range of distances from the star until the star reaches the tip of the asymptotic giant branch. If life could form and evolve over time intervals from $5 \times 10^8$ to $10^9$ years, then there could be habitable planets with life around red giant stars. For a 1 M$_{\odot}$ star at the first stages of its post main-sequence evolution, the temporal transit of the habitable zone is estimated to be of several 10$^9$ years at 2 AU and around 10$^8$ years at 9 AU. Under these circumstances life could develop at distances in the range 2-9 AU in the environment of sub-giant or giant stars and in the far distant future in the environment of our own Solar System. After a star completes its first ascent along the Red Giant Branch and the He flash takes place, there is an additional stable period of quiescent He core burning during which there is another opportunity for life to develop. For a 1 M$_{\odot}$ star there is an additional $10^9$ years with a stable habitable zone in the region from 7 to 22 AU. Space astronomy missions, such as proposed for the Terrestrial Planet Finder (TPF) and Darwin should also consider the environments of sub-giants and red giant stars as potentially interesting sites for understanding the development of life

Resolving Coparenting Dissatisfaction In Couples: A Preliminary Task Analysis Study.

This study explored the change that unfolded when parents resolved their coparenting dissatisfaction during an Integrative Brief Systemic Intervention (IBSI) for parent couples. We conducted a task analysis (Greenberg, 2007) to build a model of resolving coparenting dissatisfaction. We compared a postulated model of change (rational model) based on theoretical and clinical assumptions to the observations of the actual change process that couples experienced in an IBSI (empirical analysis). The empirical analysis was conducted on six IBSI therapy cases (three exhibiting positive development and three exhibiting no development). We defined positive development in IBSI as moving from coparenting dissatisfaction to coparenting satisfaction. The final rational-empirical model included six steps that facilitated the resolution of coparenting dissatisfaction. This study contributes to deepening the knowledge of how coparenting may change during marital therapy