Holographic Imaging of Crowded Fields: High Angular Resolution Imaging with Excellent Quality at Very Low Cost

We present a method for speckle holography that is optimised for crowded fields. Its two key features are an iterativ improvement of the instantaneous Point Spread Functions (PSFs) extracted from each speckle frame and the (optional) simultaneous use of multiple reference stars. In this way, high signal-to-noise and accuracy can be achieved on the PSF for each short exposure, which results in sensitive, high-Strehl re- constructed images. We have tested our method with different instruments, on a range of targets, and from the N- to the I-band. In terms of PSF cosmetics, stability and Strehl ratio, holographic imaging can be equal, and even superior, to the capabilities of currently available Adaptive Optics (AO) systems, particularly at short near-infrared to optical wavelengths. It outperforms lucky imaging because it makes use of the entire PSF and reduces the need for frame selection, thus leading to higher Strehl and improved sensitivity. Image reconstruction a posteriori, the possibility to use multiple reference stars and the fact that these reference stars can be rather faint means that holographic imaging offers a simple way to image large, dense stellar fields near the diffraction limit of large telescopes, similar to, but much less technologically demanding than, the capabilities of a multi-conjugate adaptive optics system. The method can be used with a large range of already existing imaging instruments and can also be combined with AO imaging when the corrected PSF is unstable.Comment: Accepted for publication in MNRAS on 15 Nov 201

Validazione di Piani di Disaster Recovery mediante Simulatore

Contribution published online at: http://www.mimos.it/nuovo/contenuto_view.asp?check=10

UV-induced expression of key component of the tanning process, the POMC and MC1R genes, is dependent on the p-38-activated upstream stimulating factor-1 (USF-1).

Protection against UV-mediated DNA damage and the onset of oncogenesis is afforded by the tanning response in which UV irradiation triggers melanocytes to increase production of melanin that is then transferred to keratinocytes. A key component of the tanning process is the UV-mediated induction of the pro-opiomelanocortin (POMC) and MC1R genes encoding the alpha-melanocyte-stimulating hormone and its receptor, respectively, which play a crucial role in pigmentation by regulating the intracellular levels of cAMP. How these genes are regulated in response to UV irradiation is not known. Here we have shown that UV-induced activation of the POMC and MC1R promoters is mediated by p38 stress-activated kinase signaling to the transcription factor, upstream stimulating factor-1 (USF-1). Importantly, melanocytes derived from USF-1 -/- mice exhibit a defective UV response and fail to activate POMC and MC1R expression in response to UV irradiation. The results define USF-1 as a critical UV-responsive activator of genes implicated in protection from solar radiation

O Knight shifts of the various types of CuO

A 17O NMR study has been performed on a 17O enriched powder sample of (Bi, Pb)2Sr2Ca2Cu3Oy, also called n = 3 phase (with Tc = 110K) which belongs to the Bi-based superconductors family (Bi, Pb)2Sr2Can-1CunO2n+4 The n = 3 compound which contains in its unit cell two types of CuO2 planes (labelled type I and II), Is compared to the n = 2 compound where only one type of CuCO2 planes (type I) is present. 17O Knight shift measurements versus temperature, in the normal phase, have allowed us to evidence the distinct behaviours of the two types of planes present in the n = 3 compound. The results are consistent with the existence of stronger electron correlations, or smaller charge carrier density in the type II planes

Modulation transfer function assessment from periodic target made= of lines or points faced with intensity variations

Communication to : AeroSense'97 - Aerospace/Defense sensing and controls, Orlando, FL (USA), April 20-25, 1997SIGLEAvailable from INIST (FR), Document Supply Service, under shelf-number : 22419, issue : a.1997 n.60 / INIST-CNRS - Institut de l'Information Scientifique et TechniqueFRFranc

ERK-regulated differential expression of the Mitf 6a/b splicing isoforms in melanoma.

The master regulator of the melanocyte lineage Mitf is intimately involved in development as well as melanoma, controlling cell survival, differentiation, proliferation and metastasis/migration. Consistent with its central role, Mitf expression and Mitf post-translational modifications are tightly regulated. An additional potential level of regulation is afforded by differential splicing of Mitf exon-6 leading to the generation of two isoforms that differ by the presence of six amino-acids in the Mitf (+) isoform and which have differential effects on cell cycle progression. However, whether the ratio of the two isoforms is regulated and whether their expression correlates with melanoma progression is not known. Here, we show that the differential expression of the Mitf 6a/b isoforms is dependent on the MAPKinase signalling, being linked to the activation of MEK1-ERK2, but not to N-RAS/B-RAF mutation status. In addition, quantification of Mitf 6a/b splicing forms in 86 melanoma samples revealed substantially increased levels of the Mitf (-) form in a subset of metastatic melanomas. The results suggest that differential expression of the Mitf 6a/b isoforms may represent an additional mechanism for regulating Mitf function and melanoma biology