A case report of delayed cortical infarction adjacent to sulcal clots after traumatic subarachnoid hemorrhage in the absence of proximal vasospasm

Background Cortical ischemic lesions represent the predominant pathomorphological pattern of focal lesions after aneurysmal subarachnoid hemorrhage (aSAH). Autopsy studies suggest that they occur adjacent to subarachnoid blood and are related to spasm of small cortical rather than proximal arteries. Recent clinical monitoring studies showed that cortical spreading depolarizations, which induce cortical arterial spasms, are involved in lesion development. If subarachnoid blood induces adjacent cortical lesions, it would be expected that (i) they also develop after traumatic subarachnoid hemorrhage (tSAH), and (ii) lesions after tSAH can occur in absence of angiographic vasospasm, as was found for aSAH. Case presentation An 86-year-old woman was admitted to our hospital with fluctuating consciousness after hitting her head during a fall. The initial computed tomography (CT) was significant for tSAH in cortical sulci. On day 8, the patient experienced a secondary neurological deterioration with reduced consciousness and global aphasia. Whereas the CT scan on day 9 was still unremarkable, magnetic resonance imaging (MRI) on day 10 revealed new cortical laminar infarcts adjacent to sulcal blood clots. Proximal vasospasm was ruled out using MR and CT angiography and Doppler sonography. CT on day 14 confirmed the delayed infarcts. Conclusions We describe a case of delayed cortical infarcts around sulcal blood clots after tSAH in the absence of proximal vasospasm, similar to results found previously for aSAH. As for aSAH, this case suggests that assessment of angiographic vasospasm is not sufficient to screen for risk of delayed infarcts after tSAH. Electrocorticography is suggested as a complementary method to monitor the hypothesized mechanism of spreading depolarizations

A novel methodology for the determination of biomarker baseline levels in the marine polychaete Hediste diversicolor

International audienceIdentifying environmental damage due to anthropogenic activities is a focal point for scientists and policy makers like those involved in the European Water Framework Directive (WFD). Many of these approaches focus on ecological endpoints for assessing environmental perturbations, which lead to policies emphasizing mitigation rather than prevention. Biomarkers provide early-warning indicators of stress but it is necessary to distinguish their natural variations from those induced by chemical stress. The global aim of this study was to establish a baseline assessment criterion (BAC) using historical data in a reference site to define toxicity thresholds. We have developed a multiple polynomial regression model (MPR) accounting the influence of salinity, temperature and size of individual on energetic reserves (glycogen and lipids) in the marine polychaete Hediste diversicolor. The model identified a complex, orthogonal relationship between confounding factors and glycogen and a linear relationship between lipids and size of individuals.Copyright © 2016 Elsevier Ltd. All rights reserved

Évaluation de la contamination et des effets écotoxiques des microplastiques en estuaire de Seine ; les premiers enseignements du projet Plastic-Seine

International audienceLe bassin versant de la Seine peut être considéré comme représentatif des bassins hydrographiques fortement impactés par l'activité humaine et à ce titre présente une forte pression de pollution. La pollution chimique constitue une menace supplémentaire pour des écosystèmes aquatiques déjà trèsfragiles tels que les estuaires et les zones marines côtières. Peu d'études se sont concentrées sur la contamination et l'impact des microplastiques (MP, taille < 5 mm) dans les écosystèmes estuariens et aucune à ce jour en estuaire de Seine. Le projet Plastic-Seine (GIP Seine-Aval et CPIER Vallée de Seine) vise d’une part à quantifier la présence et le flux de MP dans l'estuaire de la Seine et d’autre part à évaluer leur devenir et leur impact sur différentes espèces du réseau trophique. Plusieurs campagnes de terrain ont été menées depuis mars 2017 sur le continuum eau douce-eau salée à différentes saisons pour échantillonner les eaux de surface, les sédiments et sept espèces du réseau trophique (poissons, crustacés, mollusques, annélides) représentatives de l'écosystème estuarienne de la Seine. Les premiers résultats indiquent une contamination modérée (1,8-5,1 fragments/m3) mais généralisée des différents compartiments abiotiques et biotiques par des MP comprenant principalement des fibres et des fragments. Des MP ont été identifiés dans toutes les espèces analysées, quel que soit le site ou la saison de prélèvement. Des expositions à des MP environnementaux en conditions contrôlées de laboratoire ont démontré l’ingestion mais aussi l’égestion très rapide de ces MP par les annélides et la sole commune (les analyses sont en cours sur les copépodes). Des premiers effets significatifs des MP sur la survie et la reproduction du copépode E. affinis et sur le comportement des soles ont été observés. Ce projet devrait fournir d’ici 2020 une première évaluation de la distributionet du flux de microplastiques dans l'estuaire de la Seine et de la faune aquatique associée

Fate and effects of metal-based nanoparticles in two marine invertebrates, the bivalve mollusc Scrobicularia plana and the annelid polychaete Hediste diversicolor

The objective of this paper is to synthesize results from seven published research papers employing different experimental approaches to evaluate the fate of metal-based nanoparticles (Ag NPs, Au NPs, CuO NPs, CdS NPs, ZnO NPs) in the marine environment and their effects on two marine endobenthic species, the bivalve Scrobicularia plana and the ragworm Hediste diversicolor. The experiments were carried out under laboratory (microcosms) conditions or under environmentally realistic conditions in outdoor mesocosms. Based on results from these seven papers, we addressed the following research questions: (1) How did the environment into which nanoparticles were released affect their physicochemical properties?, (2) How did the route of exposure (seawater, food, sediment) influence bioaccumulation and effects?, (3) Which biomarkers were the most responsive? and (4) Which tools were the most efficient to evaluate the fate and effects of NPs in the marine environment? The obtained results showed that metal-based NPs in general were highly agglomerated/aggregated in seawater. DGT tools could be used to estimate the bioavailability of metals released from NPs under soluble form in the aquatic environment. Both metal forms (nanoparticulate, soluble) were generally bioaccumulated in both species. Among biochemical tools, GST and CAT were the most sensitive revealing the enhancement of anti-oxidant defenses in both species exposed to sub-lethal concentrations of metal-based NPs. Apoptosis and genotoxicity were frequently observed.</p