The Copper-microRNA Pathway Is Integrated with Developmental and Environmental Stress Responses in Arabidopsis thaliana

As an essential nutrient, copper (Cu) scarcity causes a decrease in agricultural production. Cu deficiency responses include the induction of several microRNAs, known as Cu-miRNAs, which are responsible for degrading mRNAs from abundant and dispensable cuproproteins to economize copper when scarce. Cu-miRNAs, such as miR398 and miR408 are conserved, as well as the signal transduction pathway to induce them under Cu deficiency. The Arabidopsis thaliana SQUAMOSA-PROMOTER BINDING PROTEIN-LIKE (SPL) family member SPL7 binds to the cis-regulatory motifs present in the promoter regions of genes expressed under Cu deficiency, including Cu-miRNAs. The expression of several other SPL transcription factor family members is regulated by miR156. This regulatory miR156-SPL module plays a crucial role in developmental phase transitions while integrating internal and external cues. Here, we show that Cu deficiency also affects miR156 expression and that SPL3 overexpressing plants, resistant to miR156 regulation, show a severe decrease in SPL7-mediated Cu deficiency responses. These include the expression of Cu-miRNAs and their targets and is probably due to competition between SPL7 and miR156-regulated SPL3 in binding to cis-regulatory elements in Cu-miRNA promoters. Thus, the conserved SPL7-mediated Cu-miRNA pathway could generally be affected by the miR156-SPL module, thereby underscoring the integration of the Cu-miRNA pathway with developmental and environmental stress responses in Arabidopsis thaliana.</i

Dynamic visual servo control of a 4-axis joint tool to track image trajectories during machining complex shapes

A large part of the new generation of computer numerical control systems has adopted an architecture based on robotic systems. This architecture improves the implementation of many manufacturing processes in terms of flexibility, efficiency, accuracy and velocity. This paper presents a 4-axis robot tool based on a joint structure whose primary use is to perform complex machining shapes in some non-contact processes. A new dynamic visual controller is proposed in order to control the 4-axis joint structure, where image information is used in the control loop to guide the robot tool in the machining task. In addition, this controller eliminates the chaotic joint behavior which appears during tracking of the quasi-repetitive trajectories required in machining processes. Moreover, this robot tool can be coupled to a manipulator robot in order to form a multi-robot platform for complex manufacturing tasks. Therefore, the robot tool could perform a machining task using a piece grasped from the workspace by a manipulator robot. This manipulator robot could be guided by using visual information given by the robot tool, thereby obtaining an intelligent multi-robot platform controlled by only one camera.This work was funded by the Ministry of Science and Innovation of Spain Government through the research project DPI2011-22766 and DPI2012-32390

Spitzer Infrared Spectrograph Observations of Magellanic Cloud Planetary Nebulae: the nature of dust in low metallicity circumstellar ejecta

We present 5 - 40 micron spectroscopy of 41 planetary nebulae (PNe) in the Magellanic Clouds, observed with the Infrared Spectrograph on board the Spitzer Space Telescope. The spectra show the presence of a combination of nebular emission lines and solid-state features from dust, superimposed on the thermal IR continuum. By analyzing the 25 LMC and 16 SMC PNe in our sample we found that the IR spectra of 14 LMC and 4 SMC PNe are dominated by nebular emission lines, while the other spectra show solid-state features. We observed that the solid-state features are compatible with carbon-rich dust grains (SiC, polycyclic aromatic hydrocarbons (PAHs), etc.) in most cases, except in three PNe showing oxygen-rich dust features. The frequency of carbonaceous dust features is generally higher in LMC than in SMC PNe. The spectral analysis allowed the correlations of the dust characteristics with the gas composition and morphology, and the properties of the central stars. We found that: 1) all PNe with carbonaceous dust features have C/O>1, none of these being bipolar or otherwise highly asymmetric; 2) all PNe with oxygen-rich dust features have C/O<1, with probable high mass progenitors if derived from single-star evolution (these PNe are either bipolar or highly asymmetric); 3) the dust temperature tracks the nebular and stellar evolution; and 4) the dust production efficiency depends on metallicity, with low metallicity environments not favoring dust production.Comment: The Astrophysical Journal, in pres

Orangulas: effect of scheduled visual enrichment on behavioral and endocrine aspects of a captive orangutan (Pongo pygmaeus)

Captivity may have adverse effects on captive great apes, as they spend much more of their time engaged in foraging and other activities in the wild. Enrichment interventions have the potential to alleviate the adverse effects of captivity by introducing novel stimuli. In orangutans (Pongo pygmaeus), interactive digital enrichment has proven effective at engaging users out of their own free will, in exchange for nothing but the experience. This article reports the results of scheduled visual enrichment in the form of “orangulas”—one-hour long videos of footage consisting mainly of open spaces in different environments, with which the pongid participant could engage at free will. The efficacy of the orangulas were measured with both behavioural and endocrine measurements, concluding that scheduled visual enrichment has the potential to improve the welfare of captive orangutans by providing novel stimuli in the context of largely stable environments

Ecological factors are likely drivers of eye shape and colour pattern variations across anthropoid primates

Action Contro

Reliability of a standing isokinetic shoulder rotators strength test using a functional electromechanical dynamometer: Effects of velocity

Indexación: Scopus.Background. The evaluation of the force in internal rotation (IR) and external rotation (ER) of the shoulder is commonly used to diagnose possible pathologies or disorders in the glenohumeral joint and to assess patient's status and progression over time. Currently, there is new technology of multiple joint isokinetic dynamometry that allows to evaluate the strength in the human being. The main purpose of this study was to determine the absolute and relative reliability of concentric and eccentric internal and external shoulder rotators with a functional electromechanical dynamometer (FEMD). Methods. Thirty-two male individuals (21.46±2.1 years) were examined of concentric and eccentric strength of shoulder internal and external rotation with a FEMD at velocities of 0.3 m s-1and 0.6 m s-1. Relative reliability was determined by intraclass correlation coefficients (ICC). Absolute reliability was quantified by standard error of measurement (SEM) and coefficient of variation (CV). Systematic differences across velocities testing circumstances, were analyzed with dependent t tests or repeatedmeasures analysis of variance in case of two or more than two conditions, respectively. Results. Reliability was high to excellent for IR and ER on concentric and eccentric strength measurements, regardless of velocity used (ICC: 0.81-0.98, CV: 5.12-8.27% SEM: 4.06-15.04N). Concentric outcomes were more reliable than eccentric due to the possible familiarization of the population with the different stimuli. Conclusion. All procedures examined showed high to excellent reliability for clinical use. However, a velocity of 0.60 m s-1should be recommended for asymptomatic male patients because it demands less time for evaluation and patients find it more comfortable. © 2020 PeerJ Inc.. All rights reserved.https://peerj.com/articles/9951

Estimation of the real population and its impact on the utilisation of healthcare services in Mediterranean resort regions: an ecological study

BACKGROUND: The demographic structure has a significant influence on the use of healthcare services, as does the size of the population denominators. Very few studies have been published on methods for estimating the real population such as tourist resorts. The lack of information about these problems means there is a corresponding lack of information about the behaviour of populational denominators (the floating population or tourist load) and the effect of this on the use of healthcare services. The objectives of the study were: a) To determine the Municipal Solid Waste (MSW) ratio, per person per day, among populations of known size; b) to estimate, by means of this ratio, the real population in an area where tourist numbers are very significant; and c) to determine the impact on the utilisation of hospital emergency healthcare services of the registered population, in comparison to the non-resident population, in two areas where tourist numbers are very significant. METHODS: An ecological study design was employed. We analysed the Healthcare Districts of the Costa del Sol and the island of Menorca. Both are Spanish territories in the Mediterranean region. RESULTS: In the two areas analysed, the correlation coefficient between the MSW ratio and admissions to hospital emergency departments exceeded 0.9, with p < 0.001. On the basis of MSW generation ratios, obtained for a control zone and also measured in neighbouring countries, we estimated the real population. For the summer months, when tourist activity is greatest and demand for emergency healthcare at hospitals is highest, this value was found to be double that of the registered population. CONCLUSION: The MSW indicator, which is both ecological and indirect, can be used to estimate the real population in areas where population levels vary significantly during the year. This parameter is of interest in planning and dimensioning the provision of healthcare services

Assessment of the load-velocity profile in the free-weight prone bench pull exercise through different velocity variables and regression models

This aims of this study were (I) to determine the velocity variable and regression model which best fit the load-velocity relationship during the free-weight prone bench pull exercise, (II) to compare the reliability of the velocity attained at each percentage of the one-repetition maximum (1RM) between different velocity variables and regression models, and (III) to compare the within- and between-subject variability of the velocity attained at each %1RM. Eighteen men (14 rowers and four weightlifters) performed an incremental test during the free-weight prone bench pull exercise in two different sessions. General and individual load-velocity relationships were modelled through three velocity variables (mean velocity [MV], mean propulsive velocity [MPV] and peak velocity [PV]) and two regression models (linear and second-order polynomial). The main findings revealed that (I) the general (Pearson's correlation coefficient [r] range = 0.964-0.973) and individual (median r = 0.986 for MV, 0.989 for MPV, and 0.984 for PV) load-velocity relationships were highly linear, (II) the reliability of the velocity attained at each %1RM did not meaningfully differ between the velocity variables (coefficient of variation [CV] range = 2.55-7.61% for MV, 2.84-7.72% for MPV and 3.50-6.03% for PV) neither between the regression models (CV range = 2.55-7.72% and 2.73-5.25% for the linear and polynomial regressions, respectively), and (III) the within-subject variability of the velocity attained at each %1RM was lower than the between-subject variability for the light-moderate loads. No meaningful differences between the within- and between-subject CVs were observed for the MV of the 1RM trial (6.02% vs. 6.60%; CVratio = 1.10), while the within-subject CV was lower for PV (6.36% vs. 7.56%; CVratio = 1.19). These results suggest that the individual load-MV relationship should be determined with a linear regression model to obtain the most accurate prescription of the relative load during the free-weight prone bench pull exercise