A Study of Cool White Dwarfs in the Sloan Digital Sky Survey Data Release 12

In this work we study white dwarfs where $30\,000\,\text{K} {>} \mathrm{T}_{\rm{eff}} {>} 5\,000\,\text{K}$ to compare the differences in the cooling of DAs and non-DAs and their formation channels. Our final sample is composed by nearly $13\,000$ DAs and more than $3\,000$ non-DAs that are simultaneously in the SDSS DR12 spectroscopic database and in the \textit{Gaia} survey DR2. We present the mass distribution for DAs, DBs and DCs, where it is found that the DCs are ${\sim}0.15\,\mathrm{M}_\odot$ more massive than DAs and DBs on average. Also we present the photometric effective temperature distribution for each spectral type and the distance distribution for DAs and non-DAs. In addition, we study the ratio of non-DAs to DAs as a function of effective temperature. We find that this ratio is around ${\sim}0.075$ for effective temperature above ${\sim}22\,000\,\text{K}$ and increases by a factor of five for effective temperature cooler than $15\,000\,\text{K}$. If we assume that the increase of non-DA stars between ${\sim}22\,000\,\text{K}$ to ${\sim}15\,000\,\text{K}$ is due to convective dilution, $14{\pm}3$ per cent of the DAs should turn into non-DAs to explain the observed ratio. Our determination of the mass distribution of DCs also agrees with the theory that convective dilution and mixing are more likely to occur in massive white dwarfs, which supports evolutionary models and observations suggesting that higher mass white dwarfs have thinner hydrogen layers.Comment: 9 pages, 10 figures, accepted by MNRA

White dwarf and subdwarf stars in the Sloan Digital Sky Survey Data Release 14

White dwarfs carry information on the structure and evolution of the Galaxy, especially through their luminosity function and initial-to-final mass relation. Very cool white dwarfs provide insight into the early ages of each population. Examining the spectra of all stars with $3\sigma$ proper motion in the Sloan Digital Sky Survey Data Release 14, we report the classification for 20 088 spectroscopically confirmed white dwarfs, plus 415 hot subdwarfs, and 311 cataclysmic variables. We obtain Teff, log g and mass for hydrogen atmosphere white dwarf stars (DAs), warm helium atmosphere white dwarfs (DBs), hot subdwarfs (sdBs and sdOs), and estimate photometric Teff for white dwarf stars with continuum spectra (DCs). We find 15793 sdAs and 447 dCs between the white dwarf cooling sequence and the main sequence, especially below Teff= 10000 K; most are likely low-mass metal-poor main sequence stars, but some could be the result of interacting binary evolution.Comment: 18 pages, 13 figure

A comparative analysis of the observed white dwarf cooling sequence from globular clusters

We report our study of features at the observed red end of the white dwarf cooling sequences for three Galactic globular clusters: NGC\,6397, 47\,Tucanae and M\,4. We use deep colour-magnitude diagrams constructed from archival Hubble Space Telescope (ACS) to systematically investigate the blue turn at faint magnitudes and the age determinations for each cluster. We find that the age difference between NGC\,6397 and 47\,Tuc is 1.98$^{+0.44}_{-0.26}$\,Gyr, consistent with the picture that metal-rich halo clusters were formed later than metal-poor halo clusters. We self-consistently include the effect of metallicity on the progenitor age and the initial-to-final mass relation. In contrast with previous investigations that invoked a single white dwarf mass for each cluster, the data shows a spread of white dwarf masses that better reproduce the shape and location of the blue turn. This effect alone, however, does not completely reproduce the observational data - the blue turn retains some mystery. In this context, we discuss several other potential problems in the models. These include possible partial mixing of H and He in the atmosphere of white dwarf stars, the lack of a good physical description of the collision-induced absorption process and uncertainties in the opacities at low temperatures. The latter are already known to be significant in the description of the cool main sequence. Additionally, we find that the present day local mass function of NGC\,6397 is consistent with a top-heavy type, while 47\,Tuc presents a bottom-heavy profile.Comment: Accepted for publication in MNRAS (16 pages, 19 figures

Freqüência e fatores de risco associados à toxocaríase em crianças de ambulatório pediátrico na região sudeste do Brasil

Para determinar a frequência de anticorpos anti-Toxocara sp. e os fatores de risco associados à infecção, soros de 242 crianças de ambos os sexos, com idade entre um a 15 anos, atendidas no ambulatório do Hospital da Universidade Federal de Uberlândia, Minas Gerais, Brasil, foram analisados pelo ELISA. Questionários foram aplicados, para obter informações sobre os pacientes. As informações sobre as alterações clínicas, de 187 pacientes, foram obtidas no prontuário médico. Do total das 242 amostras, 21 (8,7%) foram positivas para anticorpos anti-Toxocara sp.. A presença de cães e gatos e a variável escola (local de contato) sugerem associação significativa (p ; 20%) mostram correlação positiva com a soropositividade para Toxocara sp.. A idade, sexo, sintomas como dor de cabeça, dor estomacal, crises convulsivas e anemia não apresentaram associação com toxocaríase.To estimate the frequency of anti-Toxocara sp. antibodies, and evaluate factors associated with this infection, sera from 242 male and female children, aged from one to fifteen years old, attended at the Hospital of the Federal University of Uberlândia, Minas Gerais State, Brazil, were analyzed by ELISA. Information on the patients was collected and registered using an investigative questionnaire, and details on possible clinical alterations were obtained from the medical charts of 187 patients. Of a total of 242 samples, 21 (8.7%) were positive for anti-Toxocara sp. antibodies. The presence of dogs and cats and the school variable (place of contact), appeared to be significantly associated (p < 0.05) with a positive serology. Respiratory symptoms and eosinophil counts greater than 20% also showed a positive statistical correlation with a positive serology for Toxocara sp.. Factors such as sex and age, and symptoms like headache, stomach ache, convulsive crises and anemia were not associated with toxocariasis

Development and Physicochemical Characterization of Desonide-Loaded Nanocapsule Suspensions

Desonide is a topical corticosteroid that has been used for more than 30 years; however, its prolonged use can induce several side effects, affecting dermis and epidermis. The present work consists of development desonide-loaded nanocapsule suspensions (D-NC) using different polymers (Eudragit S100® or Eudragit L100®) and desonide-loaded lipid-core nanocapsules (D-LNC). They were formulated by interfacial deposition using the preformed polymer method and all formulations showed negative zeta potential and adequate nanotechnological characteristics (particle size 161–202 nm, polydispersity index < 0.20). Simple and sensitive methods using high-performance liquid chromatography (HPLC) were developed to quantify desonide in LNC and to study its release kinetics. The method was linear, specific, precise, and exact and therefore can be applied in quantification of D-NC and D-LNC. We evaluated in vitro methods for drug release (dissolution, Franz diffusion cells, and dialysis sac) and we use mathematical models (monoexponential, biexponential, and Korsmeyer-Peppas) to show release kinetics from this system

The MNT transcription factor autoregulates its expression and supports proliferation in MYC-associated factor X (MAX)-deficient cells

The MAX network transcriptional repressor (MNT) is an MXD family transcription factor of the basic helix-loop-helix (bHLH) family. MNT dimerizes with another transcriptional regulator, MYC-associated factor X (MAX), and down-regulates genes by binding to E-boxes. MAX also dimerizes with MYC, an oncogenic bHLH transcription factor. Upon E-box binding, the MYC-MAX dimer activates gene expression. MNT also binds to the MAX dimerization protein MLX (MLX), and MNT-MLX and MNT-MAX dimers co-exist. However, all MNT functions have been attributed to MNT-MAX dimers, and no functions of the MNT-MLX dimer have been described. MNT's biological role has been linked to its function as a MYC oncogene modulator, but little is known about its regulation. We show here that MNT localizes to the nucleus of MAX-expressing cells and that MNT-MAX dimers bind and repress the MNT promoter, an effect that depends on one of the two E-boxes on this promoter. In MAX-deficient cells, MNT was overexpressed and redistributed to the cytoplasm. Interestingly, MNT was required for cell proliferation even in the absence of MAX. We show that in MAX-deficient cells, MNT binds to MLX, but also forms homodimers. RNA-sequencing experiments revealed that MNT regulates the expression of several genes even in the absence of MAX, with many of these genes being involved in cell cycle regulation and DNA repair. Of note, MNT-MNT homodimers regulated the transcription of some genes involved in cell proliferation. The tight regulation of MNT and its functionality even without MAX suggest a major role for MNT in cell proliferation.This work was supported by Grant SAF2017-88026-R from Agencia Estatal de Investigación, Spanish Government (to J. L. and M. D. D.), funded in part by FEDER Program from the European Union, National Institutes of Health Grant CA57138/CA from NCI (to R. N. E.), and grants from Shriners Hospitals for Children (to P. J. H.). The authors declare that they have no conflicts of interest with the contents of this article. The content is solely the responsibility of the authors and does not necessarily represent the official views of the National Institutes of Health

Nanostructured Systems Containing Rutin: In Vitro Antioxidant Activity and Photostability Studies

The improvement of the rutin photostability and its prolonged in vitro antioxidant activity were studied by means of its association with nanostructured aqueous dispersions. Rutin-loaded nanocapsules and rutin-loaded nanoemulsion showed mean particle size of 124.30 ± 2.06 and 124.17 ± 1.79, respectively, polydispersity index below 0.20, negative zeta potential, and encapsulation efficiency close to 100%. The in vitro antioxidant activity was evaluated by the formation of free radical ·OH after the exposure of hydrogen peroxide to a UV irradiation system. Rutin-loaded nanostructures showed lower rutin decay rates [(6.1 ± 0.6) 10−3 and (5.1 ± 0.4) 10−3 for nanocapsules and nanoemulsion, respectively] compared to the ethanolic solution [(35.0 ± 3.7) 10−3 min−1] and exposed solution [(40.1 ± 1.7) 10−3 min−1] as well as compared to exposed nanostructured dispersions [(19.5 ± 0.5) 10−3 and (26.6 ± 2.6) 10−3, for nanocapsules and nanoemulsion, respectively]. The presence of the polymeric layer in nanocapsules was fundamental to obtain a prolonged antioxidant activity, even if the mathematical modeling of the in vitro release profiles showed high adsorption of rutin to the particle/droplet surface for both formulations. Rutin-loaded nanostructures represent alternatives to the development of innovative nanomedicines