Pastagens em sistemas silvipastoris.

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T Cell Priming by Activated Nlrc5-Deficient Dendritic Cells Is Unaffected despite Partially Reduced MHC Class I Levels.

NLRC5, a member of the NOD-like receptor (NLR) protein family, has recently been characterized as the master transcriptional regulator of MHCI molecules in lymphocytes, in which it is highly expressed. However, its role in activated dendritic cells (DCs), which are instrumental to initiate T cell responses, remained elusive. We show in this study that, following stimulation of DCs with inflammatory stimuli, not only did NLRC5 level increase, but also its importance in directing MHCI transcription. Despite markedly reduced mRNA and intracellular H2-K levels, we unexpectedly observed nearly normal H2-K surface display in Nlrc5(-/-) DCs. Importantly, this discrepancy between a strong intracellular and a mild surface defect in H2-K levels was observed also in DCs with H2-K transcription defects independent of Nlrc5. Hence, alongside with demonstrating the importance of NLRC5 in MHCI transcription in activated DCs, we uncover a general mechanism counteracting low MHCI surface expression. In agreement with the decreased amount of neosynthesized MHCI, Nlrc5(-/-) DCs exhibited a defective capacity to display endogenous Ags. However, neither T cell priming by endogenous Ags nor cross-priming ability was substantially affected in activated Nlrc5(-/-) DCs. Altogether, these data show that Nlrc5 deficiency, despite significantly affecting MHCI transcription and Ag display, is not sufficient to hinder T cell activation, underlining the robustness of the T cell priming process by activated DCs

"Clone Wars": Episode II - The Next Generation: The Copyright Implications relating to 3D Printing and Computer-Aided Design (CAD) Files

The future market potential of 3D printing will rest on the dissemination of Computer Aided Design (CAD) files. Without clear instructions from a CAD file, a 3D printer will not function. In fact, “a 3D printer without an attached computer and a good design file is as useless as an iPod without music”. The importance of CAD-based design files, therefore, cannot be underestimated. Drawing on UK and EU copyright laws and their application to 3D printing and CAD files, this paper will, first, question whether CAD files can be protected by copyright law before considering the copyright implications thrown up by the modification of CAD files as a result of scanning and the use of online tools. Highlighting some of the challenges for rights holders and users existent in the present law the paper advocates new business models over a premature call for stringent intellectual property laws before concluding with some recommendations for the future

Productivity in the Financial Services Sector

On 11-12 November 2008, SUERF and Banque Centrale du Luxembourg organized a conference on Productivity in the Financial Services Sector on the occasion of the tenth anniversary of the Banque Centrale du Luxembourg. The conference addressed three main themes: first, stylized facts on banks' productivity developments and the measurement of productivity; second, sources of productivity in banking; and third, the possible repercussions and consequences of the financial crisis on financial institutions' future productivity development. These three topics are taken up from various angles in the papers contained in the present volume, which represent a selection of the papers presented at the conference. Coming back to the three themes mentioned at the outset, the conference yielded some interesting results and raised many issues for further research. As regards theme 1, the papers presented overall suggest that financial integration in Europe has brought some, albeit according to some studies limited, convergence of bank efficiency among countries but on average productivity improvement has been weak. Various interesting attempts to capture banks' output were presented, but the various performance and efficiency measures yield different results. Linked to the difficulty of measuring the value of financial institutions' services, it remains far from clear what the "fair value" of a bank should be. This problem may also in part explain the very sharp ups and downs of bank stocks recently. Concerning theme 2, sources of productivity in financial services, several potentially important factors were mentioned: investments in ICT, investments in human resources, the quality of managers and remuneration policy, process effectiveness, mergers and acquisitions and economies of scale, privatizations, risk diversification versus regional and/or product specialisation, and risk-taking. Yet, no unambiguous picture emerged on which of these factors are most important. Regarding theme 3, the financial crisis may have far-reaching implications on our view of financial innovation and efficiency, on the way how to measure productivity appropriately as well as on the future development of financial institutions' productivity. First, the question arises whether the quest for productivity and profitability may under certain circumstances compromise the quality of banks' services (such as, e.g. the care invested into credit assessments and risk monitoring) and as a consequence put the stability of banks and the financial system at risk. As the recent crisis and its underlying causes suggest, there may at times be trade-offs between innovation and financial stability. If financial supervision and risk management do not keep up with financial innovation, the social value of such innovation may not be positive at all times. Financial innovation may also have blurred signals on banks? financial and risk positions, and thus have misled bank shareholders, clients and supervisors in their assessment of banks? business models and conduct of business. Second, the crisis might also affect banks? future performance: Increased government interference and stricter surveillance and capital adequacy rules might curb banks? profitability and efficiency, as measured by traditional performance indicators. However, the conventional measures of performance as presented and discussed at the conference - however varied and multifaceted they may be in trying to measure efficieny (i.e. the avoidance of unnecessary costs in the production process) and competition (the avoidance of inappropriately high profits) - basically seem to have a short-term focus. A longer-term perspective would also consider e.g. the financial institutions? solvency and the safety of deposits, as well as their stability and continued performance in periods of severe stress. Such extensions to the concept of ?performance? should certainly be explored more deeply in the light of the current crisis. In particular, bank efficiency should be considered by supervisors with a view to its influence on risk behaviour. The current debate on regulatory reform in response to the crisis also addresses the need for closer international coordination among supervisory regimes. Tighter regulatory coordination may, on the one hand, close regulatory loopholes, thus curbing banks? profit opportunities, at least in the short run. On the other hand, international harmonization of regulatory rules may generate considerable cost savings for internationally active financial institutions. By contributing to financial market integration it could also stiffen competition and in this way improve efficiency. A related issue is how the crisis will affect the size of banks in the future. Will consolidation in the sector ultimately result in fewer and bigger banks? Or will governments? and regulators? bad experience with institutions which are ?too big to fail? create pressure towards more and smaller institutions? The outcome of this may in turn have possible implications for competition and thus, ultimately, on future innovation, efficiency and productivity developments. Finally, in the coming months and years the issue of exit strategies from state intervention will have to be solved. In particular, how long should partial or full nationalisations of troubled banks last? Historical experiences vary, ranging from rather rapid re-privatisations in some cases to continued strong government influence decades. How can banks? increased reliance on government assistance be abolished and market-based incentives for productivity-enhancing strategies be restored, given the massive moral hazard created by the ? unavoidable ? government bail outs of banks? In conclusion, the conference demonstrated that various disciplines ? business administration, management, organisation and economics ? as well as different professional perspectives ? those of academia, practitioners and of policy makers ? need to be combined to do full justice to the complexity of the subject at hand. SUERF?s triple constituency and multiple-discipline approach again proved particularly suitable to approaching such a far-reaching topic

The Vortex Surface in a Three-Body Quantum System

Vortices are structures known in our daily lives and observed in a wide variety of systems, from cosmic to microscopic scales. Relatively recent studies showed that vortices could also appear in simple quantum systems. For instance, they were observed experimentally and theoretically as isolated zeros in the differential cross section in atomic ionization processes by the impact of charged particles. In this work, we show that the appearance of these quantum vortices as point structures was not due to any intrinsic property of them, but to the use of restrictive geometries in their visualization. In particular, we show that by studying the fully differential cross section for hydrogen ionization by positron impact, these vortex points are actually a manifestation of a more complex and hitherto unexplored structure, a 3D “vortex surface”.Fil: Guarda, Tamara A.. Comisión Nacional de Energía Atómica. Gerencia del Área de Energía Nuclear. Instituto Balseiro; Argentina. Comisión Nacional de Energía Atómica. Centro Atómico Bariloche; ArgentinaFil: Navarrete, Francisco. Universität Rostock; AlemaniaFil: Barrachina Tejada, Raul Oscar. Comisión Nacional de Energía Atómica. Gerencia del Área de Energía Nuclear. Instituto Balseiro; Argentina. Comisión Nacional de Energía Atómica. Centro Atómico Bariloche; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Patagonia Norte; Argentin

Intellectual Property, Open Science and Research Biobanks

In biomedical research and translational medicine, the ancient war between exclusivity (private control over information) and access to information is proposing again on a new battlefield: research biobanks. The latter are becoming increasingly important (one of the ten ideas changing the world, according to Time magazine) since they allow to collect, store and distribute in a secure and professional way a critical mass of human biological samples for research purposes. Tissues and related data are fundamental for the development of the biomedical research and the emerging field of translational medicine: they represent the “raw material” for every kind of biomedical study. For this reason, it is crucial to understand the boundaries of Intellectual Property (IP) in this prickly context. In fact, both data sharing and collaborative research have become an imperative in contemporary open science, whose development depends inextricably on: the opportunities to access and use data, the possibility of sharing practices between communities, the cross-checking of information and results and, chiefly, interactions with experts in different fields of knowledge. Data sharing allows both to spread the costs of analytical results that researchers cannot achieve working individually and, if properly managed, to avoid the duplication of research. These advantages are crucial: access to a common pool of pre-competitive data and the possibility to endorse follow-on research projects are fundamental for the progress of biomedicine. This is why the "open movement" is also spreading in the biobank's field. After an overview of the complex interactions among the different stakeholders involved in the process of information and data production, as well as of the main obstacles to the promotion of data sharing (i.e., the appropriability of biological samples and information, the privacy of participants, the lack of interoperability), we will firstly clarify some blurring in language, in particular concerning concepts often mixed up, such as “open source” and “open access”. The aim is to understand whether and to what extent we can apply these concepts to the biomedical field. Afterwards, adopting a comparative perspective, we will analyze the main features of the open models – in particular, the Open Research Data model – which have been proposed in literature for the promotion of data sharing in the field of research biobanks. After such an analysis, we will suggest some recommendations in order to rebalance the clash between exclusivity - the paradigm characterizing the evolution of intellectual property over the last three centuries - and the actual needs for access to knowledge. We argue that the key factor in this balance may come from the right interaction between IP, social norms and contracts. In particular, we need to combine the incentives and the reward mechanisms characterizing scientific communities with data sharing imperative

Beta-Cyclodextrin: Eugenol Inclusion Complexes: Characterization and Antifungal Capacity

Eugenol (EUG) is the principal component of clove essential oil. It has demonstrated excellent antifungal properties against B. cinerea, one of the most important fungus in the fresh fruit decay. However, this substance is highly volatile, thermolabile and the direct contact with a food induce undesirable changes in the organoleptic properties. For this reason, the application of EUG represents a big challenger and its encapsulation through inclusion complexes formation with β-cyclodextrin (β-CD) is presented as a solution. In this way, the aim of this work was to develop, characterize and assess the antifungal capacity of inclusion complexes β-CD:EUG. For this, co-precipitation was used as method of inclusion complexes synthesis. The quantity of entrapped EUG was determined by gaseous chromatography. The inclusion complexes were characterized by thermogravimetric analysis (TGA) and Fourier transform infrared (FTIR). Moreover, the antifungal activity was probed in a headspace system. Results showed that the EUG entrapped quantity was of 68,5 mg/g inclusion complexes. Furthermore, the characteristic peaks of EUG did not appear in inclusion complexes TGA thermogram and FTIR spectrum which confirm the effective compound encapsulation. Finally, inclusion complexes inhibited the growing of A. cinerea at 53% and avoided the fungal sporulation. These findings suggest that the β- CD:EUG inclusion complexes are suitable to use in the storage and transportation of fresh fruits to prevent their deterioration. Keywords: Botrytis cinerea, fresh fruits, headspace. Resumen El eugenol (EUG) es el principal componente del aceite esencial de clavo de olor. Éste ha destacado por su efectivo control de Botrytis cinerea, uno de los hongos más importantes que provoca la pudrición de frutas frescas. Sin embargo, esta sustancia es altamente volátil, termolábil y provoca cambios desagradables en las propiedades organolépticas del alimento si está en contacto directo con el mismo, por lo que su aplicación presenta un gran desafío. Ante esto, se propone su encapsulación a través de la formación de complejos de inclusión con β-ciclodextrina (β-CD). En este sentido, el objetivo de la presente investigación fue desarrollar, caracterizar y evaluar la capacidad antifúngica de complejos de inclusión β-CD:EUG. Éstos se sintetizaron mediante el método de co-precipitación, y la cantidad de EUG atrapada en la β-CD fue cuantificada por cromatografía de gases. Los complejos de inclusión se caracterizaron a través de análisis termogravimétrico (TGA) y espectroscospía infrarroja con transformada de Fourier (FTIR). Además, su actividad antifúngica se evaluó en un sistema de espacio de cabeza. Los resultados mostraron que la cantidad de EUG atrapada fue de 68,5 mg/g de complejo de inclusión. Además, en su termograma TGA y espectro FTIR no se observaron los picos característicos del EUG, confirmando la efectiva encapsulación del compuesto. Finalmente, los complejos de inclusión inhibieron el 53% del crecimiento de B. cinerea y evitaron su esporulación. Estos resultados permiten sugerir el uso de complejos de inclusión β-CD:EUG en el almacenamiento y transporte de frutas frescas para prevenir su deterioro. Palabras clave: Botrytis cinerea, frutas frescas, espacio de cabeza

Experimental assessment of low temperature phase change materials (PCM) for refrigerating and air conditioning applications

Refrigeration, air conditioning and heat pump sector represents between 25 and 30% of the global consumption of electricity and this figure is expected to rapidly grow due to the current trend of electrification. To increase the efficiency of these systems or to maximize the use of renewable energy, latent thermal storage systems are being studied and recommended. However, there is a lack of reliable design rules based on trustable data that could help the thermal experts to design efficient and cost-effective latent thermal energy storage. This paper follows a previous work recently published (Longo et al., 2022) where the thermo-physical and transport properties of a few low temperature PCMs (i.e. 2–9 °C of melting temperature) were measured and discussed. In the present work, the attention is focused on two other fundamental aspects: the PCMs’ compatibility with the most common construction materials and their thermal behaviour during the solid/liquid phase change. The collected results will contribute to add currently unavailable data in the literature and to highlight interesting insights on the performance of the selected PCMs

Nitric oxide and L-type calcium channel influences the changes in arterial blood pressure and heart rate induced by central angiotesin II

We study the voltage dependent calcium channels and nitric oxide involvement in angiotensin II-induced pressor effect. The antipressor action of L-Type calcium channel antagonist, nifedipine, has been studied when it was injected into the third ventricle prior to angiotensin II. The influence of nitric oxide on nifedipine antipressor action has also been studied by utilizing NW-nitro-L-arginine methyl ester (LNAME) (40 μg/0.2 μl) a nitric oxide synthase inhibitor and L-arginine (20 μg/0.2 μl), a nitric oxide donor agent. Adult male Holtzman rats weighting 200–250 g, with cannulae implanted into the third ventricle were injected with angiotensin II. Angiotensin II produced an elevation in mean arterial pressure and a decreased in heart rate. Such effects were potentiated by the prior injection of LNAME. L-arginine and nifedipine blocked the effects of angiotensin II. These data showed the involvement of L-Type calcium channel and a free radical gas nitric oxide in the central control of angiotensin II-induced pressor effect. This suggested that L-Type calcium channel of the circunventricular structures of central nervous system participated in both short and long term neuronal actions of ANG II with the influence of nitrergic system

Кинетика восстановления железа при восстановительной плавке рудоугольных окатышей

Исследовано влияние интенсивности теплообмена на кинетику восстановления железа в процессе плавки рудоугольных окатышей. Показано, что с ростом интенсивности теплообмена повышается скорость восстановительных процессов. Вследствие роста коэффициента теплообмена увеличивается глубина восстановленного слоя окатыша, существенно изменяются его структура и химический состав образующейся металлической фазы.Досліджено вплив інтенсивності теплообміну на кінетику відновлення заліза в процесі плавки рудовугільних окатишів. Показано, що при зростанні інтенсивності теплообміну підвищується швидкість відновлювальних процесів. Внаслідок зростання коефіцієнту теплообміну збільшується глибина відновленого шару окатиша, суттєво змінюються його структура та хімічний склад металевої фази, що утворюється.Influence of intensity of heat exchange is investigational on kinetics reduction of iron in the process of melting ore-coal pellets. It is rotined that speed of reduction processes rises with growth of intensity of heat exchange. Because of growth of coefficient of heat exchange the depth of the recovered layer of pellet is increased, his structure and chemical composition of appearing metallic phase changes substantially