272 research outputs found
Anthocyanins and their physiologically relevant metabolites alter the expression of IL-6 and VCAM-1 in CD40L and oxidized LDL challenged vascular endothelial cells
Scope
In vitro and in vivo studies suggest that dietary anthocyanins modulate cardiovascular disease risk; however, given anthocyanins extensive metabolism, it is likely that their degradation products and conjugated metabolites are responsible for this reported bioactivity.
Methods and results
Human vascular endothelial cells were stimulated with either oxidized LDL (oxLDL) or cluster of differentiation 40 ligand (CD40L) and cotreated with cyanidin-3-glucoside and 11 of its recently identified metabolites, at 0.1, 1, and 10 μM concentrations. Protein and gene expression of IL-6 and VCAM-1 was quantified by ELISA and RT-qPCR. In oxLDL-stimulated cells the parent anthocyanin had no effect on IL-6 production, whereas numerous anthocyanin metabolites significantly reduced IL-6 protein levels; phase II conjugates of protocatechuic acid produced the greatest effects (>75% reduction, p ≤ 0.05). In CD40L-stimulated cells the anthocyanin and its phase II metabolites reduced IL-6 protein production, where protocatechuic acid-4-sulfate induced the greatest reduction (>96% reduction, p ≤ 0.03). Similarly, the anthocyanin and its metabolites reduced VCAM-1 protein production, with ferulic acid producing the greatest effect (>65% reduction, p ≤ 0.04).
Conclusion
These novel data provide evidence to suggest that anthocyanin metabolites are bioactive at physiologically relevant concentrations and have the potential to modulate cardiovascular disease progression by altering the expression of inflammatory mediators
Human metabolism and elimination of the anthocyanin, cyanidin-3-glucoside: a 13C-tracer study
BACKGROUND: Evidence suggests that the consumption of anthocyanin-rich foods beneficially affects cardiovascular health; however, the absorption, distribution, metabolism, and elimination (ADME) of anthocyanin-rich foods are relatively unknown. OBJECTIVE: We investigated the ADME of a (13)C5-labeled anthocyanin in humans. DESIGN: Eight male participants consumed 500 mg isotopically labeled cyanidin-3-glucoside (6,8,10,3',5'-(13)C5-C3G). Biological samples were collected over 48 h, and (13)C and (13)C-labeled metabolite concentrations were measured by using isotope-ratio mass spectrometry and liquid chromatography-tandem mass spectrometry. RESULTS: The mean +/- SE percentage of (13)C recovered in urine, breath, and feces was 43.9 +/- 25.9% (range: 15.1-99.3% across participants). The relative bioavailability was 12.38 +/- 1.38% (5.37 +/- 0.67% excreted in urine and 6.91 +/- 1.59% in breath). Maximum rates of (13)C elimination were achieved 30 min after ingestion (32.53 +/- 14.24 mug(13)C/h), whereas (13)C-labeled metabolites peaked (maximum serum concentration: 5.97 +/- 2.14 mumol/L) at 10.25 +/- 4.14 h. The half-life for (13)C-labeled metabolites ranged between 12.44 +/- 4.22 and 51.62 +/- 22.55 h. (13)C elimination was greatest between 0 and 1 h for urine (90.30 +/- 15.28 mug/h), at 6 h for breath (132.87 +/- 32.23 mug/h), and between 6 and 24 h for feces (557.28 +/- 247.88 mug/h), whereas the highest concentrations of (13)C-labeled metabolites were identified in urine (10.77 +/- 4.52 mumol/L) and fecal samples (43.16 +/- 18.00 mumol/L) collected between 6 and 24 h. Metabolites were identified as degradation products, phenolic, hippuric, phenylacetic, and phenylpropenoic acids. CONCLUSION: Anthocyanins are more bioavailable than previously perceived, and their metabolites are present in the circulation fo
Search for Z′ →μ+μ- in the Lμ-Lτ gauge-symmetric model at Belle
We search for a new gauge boson Z′ that couples only to heavy leptons and their corresponding neutrinos in the process e+e-→Z′(→μ+μ-)μ+μ-, using a 643 fb-1 data sample collected by the Belle experiment at or near the ϒ(1S,2S,3S,4S,5S) resonances at the KEKB collider. While previous searches for Z′ performed a data-based estimation of the initial state radiation effect, our search for the Z′ is the first to include effects due to initial state radiation in the signal simulated samples that were used in estimating the detection efficiency. No signal is observed in the Z′ mass range of 0.212-10 GeV/c2, and we set an upper limit on the coupling strength, g′, constraining the possible Z′ contribution to the anomalous magnetic dipole moment of the muon
Measurement of eta_c(1S), eta_c(2S) and non-resonant eta' pi+ pi- production via two-photon collisions
We report the measurement of gamma gamma to eta_c(1S), eta_c(2S) to eta' pi+
pi- with eta' decays to gamma rho and eta pi+ pi- using 941 fb^{-1} of data
collected with the Belle detector at the KEKB asymmetric-energy e+e- collider.
The eta_c(1S) mass and width are measured to be M = [2984.6\pm0.7 (stat.)\pm2.2
(syst.)] MeV/c^{2} and \Gamma = [30.8^{+2.3}_{-2.2}~(stat.) \pm 2.5~(syst.)]
MeV, respectively. First observation of eta_c(2S) to eta' pi+ pi- with a
significance of 5.5sigma including systematic error is obtained, and the
eta_c(2S) mass is measured to be M = [3635.1\pm3.7~(stat.)\pm2.9~(syst.)]
MeV/c^{2}. The products of the two-photon decay width and branching fraction
(B) of decays to eta'pi+ pi- are determined to be \Gamma_{gamma gamma}B =
[65.4\pm2.6~(stat.)\pm6.9~(syst.)] eV for eta_c(1S) and
[5.6^{+1.2}_{-1.1}~(stat.)\pm1.1~(syst.)] eV for eta_c(2S). A new decay mode
for the eta_c(1S) to eta'f_0(2080) with f_0(2080) to pi+ pi- is observed with a
statistical significance of 20sigma. The f_0(2080) mass and width are
determined to be M = [2083^{+63}_{-66}~(stat.)\pm 32~(syst.)] MeV/c^{2} and
\Gamma = [178^{+60}_{-178}~(stat.) \pm 55~(syst.)] MeV. The cross sections for
gamma gamma to eta' pi+ pi- and eta'f_{2}(1270) are measured for the first
time.Comment: 19 pages, 14 figure
Measurement of the lepton polarization and in the decay with one-prong hadronic decays at Belle
With the full data sample of pairs recorded by
the Belle detector at the KEKB electron-positron collider, the decay is studied with the hadronic
decays and . The polarization in two-body hadronic
decays is measured, as well as the ratio of the branching fractions , where
denotes an electron or a muon. Our results, and , are consistent with the theoretical
predictions of the Standard Model. The polarization values of are excluded at the 90\% confidence level.Comment: 17 pages, 11 figures, submitted to Physical Review
Measurement of Branching Fractions of Hadronic Decays of the Baryon
Using a data sample of 980 of annihilation data
taken with the Belle detector operating at the KEKB asymmetric-energy
collider, we report the results of a study of the decays of the
charmed baryon into hadronic final states. We report the most precise
measurements to date of the relative branching fractions of the
into , , , and
, as well as the first measurements of the branching fractions
of the into , , and , all with respect to the decay. In
addition, we investigate the resonant substructure of these modes. Finally, we
present a limit on the branching fraction for the decay
Inclusive study of bottomonium production in association with an meson in annihilations near
We study bottomonium production in association with an meson in
annihilations near the , at a center of mass energy of
GeV. The results are based on the fb data
sample collected by the Belle experiment at the asymmetric energy KEKB
collider. Only the meson is reconstructed and the missing-mass spectrum
of candidates is investigated. We observe the
process and find evidence for the
process, while no significant signals of
, , nor are found. Cross sections for the
studied processes are reported.Comment: Submitted to EPJ-
Evidence for Isospin Violation and Measurement of Asymmetries in
We report the first evidence for isospin violation in and
the first measurement of difference of asymmetries between and . This analysis is based on the data sample
containing pairs that was collected with the Belle
detector at the KEKB energy-asymmetric collider. We find evidence for
the isospin violation with a significance of 3.1, \%, where
the third uncertainty is due to the uncertainty on the fraction of to
production in decays. The measured value is
consistent with predictions of the SM. The result for the difference of
asymmetries is \%, consistent with zero. The measured branching fractions and
asymmetries for charged and neutral meson decays are the most precise to
date. We also calculate the ratio of branching fractions of to .Comment: 11 pages, 7 figures. shown at FPCP2017. accepted by PR
Measurements of the absolute branching fractions of and at Belle
We present the measurement of the absolute branching fractions of and decays, using a
data sample of pairs collected at the
resonance with the Belle detector at the KEKB asymmetric-energy
collider. Here, denotes , ,
, , , , , ,
and . We do not observe significant signals for nor
, and set the 90 confidence level upper limits: and . These represent the most stringent upper limit for
to date and the first measurement for
. The measured branching fractions for
and are the most precise to date: and , where the first and
second uncertainties are statistical and systematic, respectively.Comment: 10 pages, 3 figure
- …