Impacts des systèmes de culture en semis direct avec couverture végétale (SCV) sur la dynamique de l'eau, de l'azote minéral et du carbone du sol dans les cerrados brésiliens

Dans les conditions tropicales humides des cerrados brésiliens, de nouveaux systèmes de culture étaient nécessaires pour assurer une production agricole durable. Des systèmes en semis direct avec couverture végétale (SCV) ont été mis au point en incorporant des plantes de couverture avant ou après la culture commerciale. Depuis 1999, différentes études ont été réalisées dans le cadre d'un projet de recherche franco-brésilien. Elles ont permis de quantifier les effets de ces SCV sur les dynamiques de l'eau, de l'azote et du carbone en particulier. Il a été ainsi confirmé que les SCV permettent de diminuer le ruissellement et donc l'érosion qui lui est liée. De même, les SCV permettent une fourniture en azote supérieure pour la plante commerciale. L'eau et l'azote non valorisées par cette culture peuvent l'être par la plante de couverture additionnelle, de même que l'eau et l'azote mis à disposition en dehors de ce cycle commercial. Les importantes restitutions de phytomasse de ces nouveaux systèmes à plusieurs plantes dans l'année aboutissent à un bilan de carbone positif et une augmentation des taux de matière organique des sols. (Résumé d'auteur

Impacts des systèmes de culture en semis direct avec couverture végétale (SCV) sur la dynamique de l'eau, de l'azote minéral et du carbone du sol dans les cerrados brésiliens.

Under the humid tropical climate of the Brazilian cerrados, new cropping systems have become necessary to ensure sustainable agricultural production. Direct-sowing mulch-based cropping systems (DMC) introduce a cover crop before or just after the commercial crop. They help farmers enhance natural resources and produce more total biomass during the year. Crop residues protect the soil and increase organic restitution to the system. In 1999, a Franco-Brazilian collaborative project began scientific studies aimed at quantifying the impact of DMC, especially on the dynamics of water, carbon and nitrogen. DMC systems significantly decrease water runoff. In this way they effectively fight against soil erosion, even if additional infiltrated water can sometimes saturate the system and escape by drainage. The cover crop can then recycle some of that drainage loss and protect and use water resources better throughout the year. DMC systems also provide more favourable conditions that ensure the more intensive and more regular mineralisation of soil organic matter during the rainy season. Some of the organic mulch mineralises at the same time, providing the commercial crop with the benefit of a higher pool of inorganic nitrogen. The additional cover crop can recycle part of the nitrogen not used by the commercial crop and some of the nitrogen mineralised before or after this commercial cycle. Under DMC high microbial activity is responsible for higher CO2 emissions to the atmosphere, but the soil carbon balance is nonetheless positive because of the large biomass production and organic input from the introduction of the cover crop. Even under the hot and humid conditions of the cerrados, soil carbon content increases under the more productive DMC systems

The tomato Prf complex is a molecular trap for bacterial effectors based on Pto transphosphorylation

The bacteria Pseudomonas syringae is a pathogen of many crop species and one of the model pathogens for studying plant and bacterial arms race coevolution. In the current model, plants perceive bacteria pathogens via plasma membrane receptors, and recognition leads to the activation of general defenses. In turn, bacteria inject proteins called effectors into the plant cell to prevent the activation of immune responses. AvrPto and AvrPtoB are two such proteins that inhibit multiple plant kinases. The tomato plant has reacted to these effectors by the evolution of a cytoplasmic resistance complex. This complex is compromised of two proteins, Prf and Pto kinase, and is capable of recognizing the effector proteins. How the Pto kinase is able to avoid inhibition by the effector proteins is currently unknown. Our data shows how the tomato plant utilizes dimerization of resistance proteins to gain advantage over the faster evolving bacterial pathogen. Here we illustrate that oligomerisation of Prf brings into proximity two Pto kinases allowing them to avoid inhibition by the effectors by transphosphorylation and to activate immune responses

Impactos do sistema de plantio direto com cobertura vegetal (SPDCV) sobre a dinâmica da água, do nitrogênio mineral e do carbono do solo do cerrado brasileiro.

Nas regiões tropicais, principalmente no cerrado brasileiro, para assegurar uma produção agrícola sustentável, é preciso recorrer a novos sistemas de cultura. Por isso, foi desenvolvido o Sistema de Plantio Direto com Cobertura vegetal, que preconiza o não-revolvimento do solo e o cultivo de plantas de cobertura, antes ou depois da cultura comercial, para produzir mais biomassa e proteger permanentemente o solo

A global spectral library to characterize the world's soil

Soil provides ecosystem services, supports human health and habitation, stores carbon and regulates emissions of greenhouse gases. Unprecedented pressures on soil from degradation and urbanization are threatening agro-ecological balances and food security. It is important that we learn more about soil to sustainably manage and preserve it for future generations. To this end, we developed and analyzed a global soil visible-near infrared (vis-NIR) spectral library. It is currently the largest and most diverse database of its kind. We show that the information encoded in the spectra can describe soil composition and be associated to land cover and its global geographic distribution, which acts as a surrogate for global climate variability. We also show the usefulness of the global spectra for predicting soil attributes such as soil organic and inorganic carbon, clay, silt, sand and iron contents, cation exchange capacity, and pH. Using wavelets to treat the spectra, which were recorded in different laboratories using different spectrometers and methods, helped to improve the spectroscopic modelling. We found that modelling a diverse set of spectra with a machine learning algorithm can find the local relationships in the data to produce accurate predictions of soil properties. The spectroscopic models that we derived are parsimonious and robust, and using them we derived a harmonized global soil attribute dataset, which might serve to facilitate research on soil at the global scale. This spectroscopic approach should help to deal with the shortage of data on soil to better understand it and to meet the growing demand for information to assess and monitor soil at scales ranging from regional to global. New contributions to the library are encouraged so that this work and our collaboration might progress to develop a dynamic and easily updatable database with better global coverage. We hope that this work will reinvigorate our community's discussion towards larger, more coordinated collaborations. We also hope that use of the database will deepen our understanding of soil so that we might sustainably manage it and extend the research outcomes of the soil, earth and environmental sciences towards applications that we have not yet dreamed of

Elevated ratio of acylated to unacylated ghrelin in children and young adults with Prader–Willi syndrome

Prader–Willi syndrome (PWS) is characterized by a switch from failure to thrive to excessive weight gain and hyperphagia in early childhood. Hyperghrelinemia may be involved in the underlying mechanisms of the switch. The purpose of this study is to evaluate acylated ghrelin (AG) and unacylated ghrelin (UAG) levels in PWS and investigate their associations with hyperphagia. This is a cross-sectional clinical study conducted in three PWS expert centers in the Netherlands and France. Levels of AG and UAG and the AG/UAG ratio were determined in 138 patients with PWS (0.2–29.4 years) and compared with 50 age-matched obese subjects (4.3–16.9 years) and 39 healthy controls (0.8–28.6 years). AEBSF was used to inhibit deacylation of AG. As a group, PWS patients had higher AG but similar UAG levels as healthy controls (AG 129.1 vs 82.4 pg/ml, p = 0.016; UAG 135.3 vs 157.3 pg/ml, resp.), resulting in a significantly higher AG/UAG ratio (1.00 vs 0.61, p = 0.001, resp.). Obese subjects had significantly lower AG and UAG levels than PWS and controls (40.3 and 35.3 pg/ml, resp.), but also a high AG/UAG ratio (1.16). The reason for the higher AG/UAG ratio in PWS and obese was, however, completely different, as PWS had a high AG and obese a very low UAG. PWS patients without weight gain or hyperphagia had a similar AG/UAG ratio as age-matched controls, in contrast to those with weight gain and/or hyperphagia who had an elevated AG/UAG ratio. The switch to excessive weight gain in PWS seems to coincide with an increase in the AG/UAG ratio, even prior to the start of hyperphagia

Crystallization and X-ray diffraction analysis of the C-terminal domain of the flax rust effector protein AvrM

The flax rust effector AvrM is a secreted protein of unknown fold that is recognized by the M resistance protein in flax. In order to investigate the structural basis of the AvrMM interaction and possible virulence-associated functions of AvrM, the C-terminal domains of two different AvrM variants (AvrM-A and avrM) were crystallized. Crystals of native AvrM-A were obtained using pentaerythritol ethoxylate (15/4 EO/OH) as a precipitant and diffracted X-rays to 2.9 angstrom resolution. Selenomethionine-derivative crystals of similar quality were obtained using PEG 1500 as a precipitant. Both the native and selenomethionine-labelled AvrM-A crystals had symmetry of space group C2221 with eight molecules in the asymmetric unit. Crystals of avrM had symmetry of space group P212121 and diffracted X-rays to 2.7 angstrom resolution. Initial AvrM-A phases were calculated using the single-wavelength anomalous dispersion (SAD) method and a partial model was built. Phases for avrM were obtained by molecular replacement using the partial AvrM-A model