产胞外布雷菲德菌素A青霉发酵条件的研究

通过正交设计试验,优化了青霉(Penicillium)属真菌SHZK-15产胞外布雷菲德菌素(B refeld in-A,简称BFA)的液体发酵条件。最佳的BFA发酵条件为:培养基含马铃薯200g(煮汁),葡萄糖20g,(NH4)2SO44.0g,KH2PO41.0g,MgSO4.7H2O 2.0g,CaCO35.0g,pH自然,瓶装量为100mL/250 mL,培养温度为28℃,转速为120 r/m in,培养时间为7d,BFA的最高产量可达151.6mg/L

中药沐足结合护理干预治疗护理人员下肢静脉曲张的效果观察

目的探讨中药沐足结合护理干预在下肢静脉曲张护士中的应用效果。方法选取某三甲综合性医院患有下肢静脉曲张的临床护士60例为研究对象,应用随机数字表法分为两组,各30例。两组研究对象均行基础护理,对照组采用医用的循序减压弹力袜进行治疗,观察组实施中药沐足结合护理干预措施。2个月后比较两组患者疗效。结果观察组研究对象静脉曲张恢复总有效率高于对照组,观察组研究对象经过3个疗程的治疗,痊愈12例,显效l3例,有效4例;对照组分别为痊愈5例,显效8例,有效9例;观察组、对照组临床治愈率和总有效率分别为40.00%、96.67%和16.67%、73.33%。差异有统计学意义(P<0.05)。结论中药沐足结合护理干预可以改善下肢静脉曲张的临床症状。福建中医药大学2016年临床专项校管课题(XB2016085

用HPLC-MS-MS快速分析和鉴定三尖杉植物内生真菌发酵液中的Brefeldin A

采用HPLC -MS -MS联用技术 ,分析了C56和C65两株具有抗肿瘤活性的三尖杉植物内生真菌发酵液抽提物 ,首次报道了这两株真菌都能产生BrefeldinA(BFA)。采用ESI-MS总离子流跟踪分析HPLC的洗脱液 ,并用低能量的CID -MS -MS(碰撞诱导裂解方式 )进一步确定目标离子峰为BFA分子离子峰 ,这为植物内生真菌发酵液中的有效成分的早期鉴别奠定了基

The study of inhibitoru eFFect of Hg--(2+) on respiratory movement of grouper (Epinephelua akaara)

本文用微机记录、分析,实验数据用勒让特正交多项式拟合处理,研究致死浓度Hg--(2+)溶液(0.5、1Mg/l)对石斑鱼呼吸运动的抑制作用,初步探讨Hg--(2+)中毒致死的可能机理。Grouper (Epinephelua akaara)was used to study the inhibitory eFFect of respiratory movement on lethal concentration of mercury (0.5,1mg/l).The experimental data were recorded and analyzed by computer, then the data were counted by Legender orthogonal polynomial.The paper discusses the probably mechanism of the death

从非医类大学生对肺结核知识认知情况的调查谈高校健康教育

健康教育不仅是学校基础教育的重要组成部分,而且在素质教育中起着不可替代的重要作用。本研究以广西某高校学生为对象,利用问卷调查的方式调查他们对肺结核的病认知和态度,通过统计分析,不同专业、性别学生对肺结核的认知和态度有差异,整体认识水平有待提高。广西民族大学青年基金项目(2011MDQW035)的阶段性成

p300/CBP and p300/CBP-associated Factor with Transcriptional Regulation

P300/CbP及其相关因子PCAf具有乙酰转移酶活性,能通过乙酰化组蛋白和非组蛋白的方式参与基因的转录调控.同时,它们也能介导多种转录因子.与大多数典型的转录辅激活子相同,它们能在转录因子和基本转录复合物之间起到桥梁作用.P300/CbP与细胞周期调控、细胞凋亡以及癌症的发生等过程之间有着直接的联系.本文概括介绍了P300/CbP与PCAf的基本特性,并简要介绍它们与其它蛋白之间的相互作用,特别是E1A的最新研究进展.p300/CBP and p300/CBP-associated factor(PCAF) exert an intrinsic histone acetyltransferase(HAT) activity.Histones and nonhistone proteins were acetylated to regulate transcriptions of specific sets of genes and interact with various transcription factors.Like the most classical transcriptional coactivators,p300/CBP and PCAF can also bridge the transcriptional factors to the basal transcriptional complex to maintain the appropriate levels of gene activities during various physiological processes,such as cell cycle,apoptosis and carcinogenesis.The review focuses on the basic characteristics of p300/CBP and PCAF,and the specific interactions with their protein partners in tumor progression,such as E1A,Rb and p53 are also discussed.国家自然科学基金专项基金项目(No.30840027)---

Inhibition of Cu 2+ on GoldFish Feeding Behavior

研究四种不同浓度Cu2＋对金鱼摄食行为、学习和记忆能力的影响，及摘除嗅囊后摄食行为的改变，探讨其中毒的机理.实验结果：1）不同浓度Cu2＋液对金鱼摄食行为呈抑制效应，抑制的程度随Cu2＋浓度的增加而加深，表现为首次撞击食球的时间（下简称首次撞击时间）随着中毒的加深而推迟，摄食的频率亦随之降低.反映了中毒鱼学习记忆的能力都随着Cu2＋浓度的增加而减弱；2）中毒鱼体重增长率随Cu2＋浓度的增加而减少，甚至呈现负增长现象；3）摘除嗅囊鱼的摄食频率仅为正常的1/2，学习和记忆的能力也较差The eFFects of Cu 2+ on the Feeding behavior, ability of learning and memory of goldFish are studied, and the changes of Feeding behavior and ability of learning and memory are investigated aFter the olFactory oragn of Fish was excised.The results are as Follows: 1) the Feeding behavior of Fish is inhibited by diFFerent concentration of Cu 2+ , the degree of inhibition increases with the increase in Cu 2+ concentration while the ability of learning and memory decrease;2) during the hole experiment period,the weight of normal Fish increased, while the weight of poisoned Fish did not.In some case,the weight of poisoned Fish increased negatively; 3)the Feeding Frequency of operative Fish are lower than that of normal Fish.The probable poisoning mechanisms are also discussed.福建省自然科学基

EFFects of Cu-(2+) and Zn-(2+) on opercular movement in Tilapia sp.

本文研究结果表明：（1）水体中含Cu2+和zn2+的浓度越高，鱼中毒越深，鳃盖运动振幅的增大和频率的减小就越显著。（2）Cu2+/zn2+混合液对鳃盖运动的影响具明显的协同作用，它的致毒效应分别为单一因子的3~5倍。（3）毒性次序：5x10-6Cu2+/50x10-6zn2+＞2.5x10-6Cu2+/25x10-6zn2+＞5x10-6Cu2+＞50x10-6zn2+（V/V）。（4）频繁的咳嗽反应是zn2+中毒的主要症状，驱逐反射是Cu2+中毒的主要症状，两者均可作为单一和混合重金属离子污染的敏感生物学监测指标。The results From the study on the eFFects of Cu2+,Zn2+ or their mixed liquid on opercular movement of Fish are as Follows: (1) The higher the Cu2+ or Zn2+ concentration, the deeper the Fish was poisoned.It is quite obvious that the amplitude of opercular movement increased and decreased in Frequency.(2) Apparently there is synergism From the eFFects of Cu2+/Zn2+mixed solution on opercular movement.The toxic eFFicacy is 3-5 times than that of single ion.(3) The sequence of toxicity is 5 ×10-6Cu2+/50 ×10-6Zn2+>2.5 ×10-6Cu2+/25 × 10-6Zn2+>5 × 10-6Cu2+ >50 × 10-6Zn2+ (V/V).(4)Cough response is the main sympton of Zn2+,expulsion reFlex is the symptom of Cu2+.Both cough response and expulsion reFlex may be used as biological index of heavy metal pollution.福建省自然科学基

Determination of phthalic acid esters in soil samples by gas chromatography-mass spectrometry with internal standard method

建立了土壤中11种酞酸酯(PAEs)的气相色谱-质谱联用(GC-MS)内标分析方法,通过优化色谱柱升温程序和载气流量后,11种酞酸酯在18 min内得到良好的分离.同时以连续7次进样后计算PAEs峰面积响应值和峰面积变异系数为衡量指标,并逐个优化GC-MS的进样口温度、GC与MS的接口温度、进样方式和进样体积等GC-MS仪器操作参数,确定了GC-MS分析PAEs的最优条件为进样口温度250℃,接口温度280℃,载气流速1.2 mL.min-1,进样体积1.0μL,非脉冲进样方式.该分析条件下PAEs各组分的检出限(S/N=3)为0.37—1.97μg.L-1.该分析方法用于土壤样品中酞酸酯含量..