Association between Methylenetetrahydrofolate Reductase Gene Single Nucleotide Polymorphisms and Lower Extremity Atherosclerotic Disease

目的:研究汉族人群中亚甲基四氢叶酸还原酶(methylene-tetrahydrofolate reductase,MTHFR)基因多态性与下肢动脉粥样硬化(lower extremity atherosclerotic disease,LEAD)的相关性。方法:收集福建省闽南地区384例(LEAD者224例,健康者160例)的临床资料及外周血;LEAD检查采用踝肱指数(ABI)、趾肱指数(TBI)、多普勒彩超和其他影像学检查等手段;选取MTHFR基因rs1801133、rs1801131、rs2274976、rs4846048、rs3737966、rs1537515、rs4846049、rs3834044、rs13306561和rs3737964等10个单核苷酸多态性(single nucleotide polymorphisms,SNP)位点进行基质辅助激光解吸电离飞行时间质谱分析技术(matrix-assisted laser desorption ionization-time of flight,MALDI-TOF)的基因分型。结果:10个SNP位点均符合HardyWeinberg平衡;rs4846048与rs3737966等37个位点之间存在明显连锁不平衡现像(D'均大于0.9);MTHFR基因GCCTCGGAAT单倍型在LEAD和正常组的分布差异有统计学意义(P=0.02);等位基因频率的χ~2检验显示rs1801131(OR=1.287);rs4846048(OR=1.844,P=0.02);rs3737966(OR=1.339);rs4846049(OR=1.314)和rs3737964(OR=1.522);且rs4846048位点的趋势χ~2检验(cochran-armitage trend test,TREND)、显性基因检验(Dominant gene action test,DOM)均显示LEAD与正常组之间分布频率的差异有统计学意义(P 0. 9) and so on. There were significant differences( P = 0. 02) in GCCTCGGAAT haplotypes of MTHFR gene groups between LEAD cases and the normal groups. The results from chi-square test of allele frequencies suggested rs1801131( OR = 1. 287),rs4846048( OR = 1. 844,P = 0. 02),rs3737966( OR =1. 339),rs4846049( OR = 1. 314) and rs3737964( OR = 1. 522). Significant differences( P < 0. 05) were observed between LEAD and the normal groups in Cochran- Armitage trend test and Dominant gene action test of rs4846048. Conclusion: The SNP of rs1801131,rs4846048,rs3737966,rs4846049 and rs3737964 might be associated with the susceptibility of LEAD,and rs4846048 gene mutation might serve as a risk factor for LEAD in the community-based population.2013年度南京军区医学科技创新课题(编号:MS098);; 解放军第175医院青年苗圃基金项目(编号:13Y015

LD端面泵浦固体激光谐振腔的研究

为了对激光谐振腔体进行优化设计,采用ABCD光学传输矩阵理论,计算得到具有普适性的三镜折叠腔内高斯模式束腰的解析解.对一典型平平热透镜腔进行计算分析,结果表明:在针对其进行优化设计的泵浦范围内,腔体稳定性很好,激光光斑半径基本不随泵浦功率变化而波动.实验中以808nm尾纤输出的激光二极管端面泵浦Nd:YAG晶体产生连续1064nm激光,激光输出结果与理论模型值能够较好地相符

Road Dust Loading and Chemical Composition at Major Cities in Fujian Province

采集并分析了福建省重点城市厦门、漳州、泉州和莆田城区具有代表性的57个地面扬尘和16个土壤样品的26种无机元素、8种水溶性离子和碳成分等组成.不同城市粒径≤100μM的路面尘负荷平均值在6.99 g.M-2与10.11 g.M-2之间,而粒径≤2.5μM的路面尘(PM2.5)负荷平均值在4.0 Mg.M-2和12.5 Mg.M-2之间.不同城市土壤尘和路面尘PM2.5中浓度最高的元素都是SI、CA、Al、fE和k,路面尘中CA元素明显富集,而主要人为来源的重金属元素如Cu、Pb、zn、Cr在路面尘PM2.5中的浓度显著高于土壤尘.漳州城区路面尘PM2.5中二次离子nH4+、nO3-和SO24-的含量明显高于其它地区,厦门、漳州和泉州城区路面尘PM2.5中Mg2+和CA2+之间都存在显著的正相关关系.四城市路面尘中有机碳(OC)的含量均高于济南、石家庄以及北京地面扬尘OC的含量,元素碳(EC)的含量均低于北京地区路面扬尘EC的含量.泉州和莆田两地OC和EC的相关性较好,说明路面尘中OC和EC有相同或相似的来源.质量平衡结果显示泉州和莆田路面尘PM2.5中含量最高的是土壤尘,其次为有机物.按化学组成特征对路面尘PM2.5进行聚类解析,得到受大气沉降影响、受土壤尘影响、受大气沉降和土壤尘共同作用以及受土壤尘和建筑尘共同作用4类样品.A total of 57 road dust and 16 urban soil samples were collected from four cities,Xiamen,Zhangzhou,Quanzhou and Putian in Fujian Province,China.Twenty-six elements,eight water soluble ions,organic carbon and elemental carbon in the fraction of particulate diameter less than 2.5 μm(PM2.5) derived through a suspension chamber were analyzed.The average loading of road dust with diameter less than 100 μm in the four cities ranged from 6.99 g·m-2 to 10.11 g·m-2,while the loading of PM2.5 ranged from 4.0 mg·m-2 to 12.5 mg·m-2.Both the soil and road dust samples were characterized with much higher concentrations of Si,Ca,Al,Fe and K.But for the anthropogenic elements such as Cu,Pb,Zn,Cr and Ti,much lower levels were found in the soil PM2.5 than those in the road dust PM2.5.Significantly higher levels of NH+4,NO-3 and SO2-4 were found in the road dust PM2.5 from Zhangzhou in comparison with those from other cities in this study.The calcium ion(Ca2+) content was significantly positively correlated with the Mg2+ content in the road dust PM2.5 from Xiamen,Zhangzhou and Quanzhou.The levels of organic carbon(OC) in the road dust PM2.5 in these four cities were higher than those reported in Ji'nan,Shijiazhuang and Beijing while the levels of elemental carbon(EC) were all lower than those in the urban road dust from Beijing,Significant positive correlation between EC and OC was found in samples from Quanzhou and Putian,suggesting the same and/or similar sources.The result of mass balance indicated that higher percentage compositions were soil and OM in both Quanzhou and Putian.Based on the cluster analysis,the 57 road dust samples were divided into four types: influenced by atmospheric deposition,influenced by soil dust,influenced by atmospheric deposition and soil dust,and influenced by soil and construction dust.国家自然科学基金项目(40971257;41171365); 环境保护公益性行业科研专项(201009004); 福建省自然科学基金计划项目(2009J05106

聚合条件对乙烯-1-辛烯共聚反应及性能影响

以甲苯为溶剂,采用自制茂金属催化剂、助催化剂三异丁基铝[Al（iBu）3]和三苯碳四（五氟苯基硼）[Ph3C]+[B（C6F5）4]-催化乙烯与1-辛烯共聚,探究了聚合温度和1-辛烯浓度对乙烯-1-辛烯共聚反应以及共聚物结构与性能的影响。结果表明:当聚合温度从100℃升高到150℃时,催化活性下降,共聚物相对分子质量持续降低,其分布则变宽,熔融温度和结晶度均上升;当1-辛烯浓度从0升高到1.2 mol/L时,催化活性显著增大,共聚物相对分子质量分布变宽,熔融温度和结晶度均下降

厦门市某医院体检人群中有机氯农药暴露与胆石症发病的关联性研究

目的探讨厦门市第二医院体检人群中有机氯农药暴露与胆结石发生的关联及其发病危险因素。方法收集2015年2月-8月在医院健康体检人群中经B超确诊的新发胆囊结石患者100例,以同期参加体检的非胆囊结石者为对照,进行1∶2配比的病例对照研究。进行流行病学调查,采用气相色谱联用电子捕获器检测研究对象外周血中有机氯农药水平。应用多因素条件Logistic回归模型分析有机氯农药与胆囊结石发病的关系。结果病例组血清中α-HCH残留水平高于对照组,差异有统计学意义;Logistic回归分析非酒精性脂肪肝、2型糖尿病、血清α-HCH含量增高可能增加胆结石的患病危险,OR值及95%CI分别为5.134(1.952~13.502)、4.754(1.849~12.220)和1.357(1.094~1.854)。结论环境有机氯农药暴露可能是胆囊结石病发生的危险因素。厦门市科技计划项目(3502Z20163006)；厦门市集美区科技计划项目(20142C01)；厦门大学校长基金(20720152012)

基于生物质谱技术的磷酰化修饰策略在多肽测序中的应用

该文建立了一种利用磷酰化修饰结合电喷雾质谱(ESI-Q-TOF)测定多肽氨基酸序列的有效方法。利用Atherton-Todd反应,以二丙基亚磷酰酯(DPP)为磷酰化试剂,应用生物质谱技术,对磷酰化修饰后的5种模型肽的磷酰化反应情况进行了系统研究,考察了磷酰化肽的二级质谱特征,并与未经磷酰化反应的肽的二级质谱特征对比。结果表明,经过磷酰化修饰后,肽的二级质谱中的a1离子信号强度明显增加,可以准确鉴定其N端氨基酸;b系列离子信息完整,信号强度增强,使得多肽C ID测序的谱图简单、清晰,有利于肽的氨基酸序列的测定;赖氨酸(K,128.10 u)和谷氨酰胺(Q,128.13 u)两种氨基酸质荷比相近,由于二者磷酰化修饰后的差异性,使其得到准确区分。经过5种已知氨基酸序列的模型肽的磷酰化后结合质谱技术进行氨基酸序列测定验证,结果表明该方法简单、快速、准确,提高了利用质谱技术进行多肽测序的准确度和灵敏度,可为蛋白质组学研究提供有效的技术手段

采用茂金属催化剂制备乙烯-1-辛烯共聚物及其性能研究

采用自制单茂金属催化剂MXC,经Al（i-Bu）3和[Ph3C]+[B（C6F5）4]-活化后对乙烯和1-辛烯进行了催化共聚,利用核磁共振碳谱(13C-NMR)、凝胶渗透色谱（GPC）、差示扫描量热法（DSC）考察了聚合条件对催化剂催化性能的影响,对比了共聚物样品以及进口韩国SK公司的聚烯烃弹性体（POE）产品。结果表明:共聚物样品与SK公司的POE产品在13C-NMR中有相似的吸收峰,组成及聚集态结构相近;随催化剂Al/Ti比（摩尔比,下同）减小,催化活性增加,共聚物的重均相对分子质量（■w）先减小后增大;聚合温度从90℃升高至130℃,催化活性降低,共聚物的相对分子质量减小,相对分子质量分布指数（PDI）变宽;当Al/Ti比为300时,共聚物■w由24.36×104降至9.87×104;当Al/Ti比为150,聚合温度为110℃时,催化活性达到11.77×106g/（mol·h）,共聚物■w,PDI,熔融温度、结晶度依次为10.05×104, 3.14, 76.95℃, 2.27%,1-辛烯插入率达到13.2%

ST段抬高型急性心肌梗死院前溶栓治疗中国专家共识

急性心肌梗死仍然严重威胁我国人民健康,在我国广大城乡地区,形势更为严峻[1,2]。及时救治急性心肌梗死患者,降低死亡率和保护心脏功能刻不容缓。鉴于我国的实际情况,院前溶栓治疗在大城市以外的城乡地区具有重要意义。为此,中国医师协会胸痛专业委员会及中国医学救援协会心血管急救分会专门组织有关专家制订了本共识,旨在帮助院前医疗急救人员对急性心肌梗死患者选择最佳

学校流感暴发疫情防控措施动力学模型效果分析

目的探讨学校流感暴发疫情的最佳控制方式,为制定停课标准提供参考。方法通过SEIAR模型对流感暴发疫情隔离和停课效果进行分析。结果本起流感暴发疫情基本再生数R0为7.6180,无干预措施下疫情会持续20天,1 169名学生和教职工（99.74%）会感染流感。第5～10天开始隔离措施,疫情持续时间介于35～44天,第10天开始隔离感染人数是第5天开始隔离的6.9倍。与单独采取隔离措施相比,停课1天、3天、5天、7天可以减少4.51%～10.92%、13.41%～29.31%、19.46%～45.40%和24.82%～57.47%的感染人数,持续时间并没有太大变化;停课14天疫情持续时间都会明显下降,感染人数下降33.43%～65.52%。结论隔离措施越早实施对疫情控制效果越好,显性感染人数达到20%以上时可以停课,出现重症或死亡病例时要将停课时间延长

Proteomic Analysis of Rice Cultivar Jiafuzhan in the Responses to Xanthomonas campestris pv.oryzicola Infection

作者简介: 陈芳育(1978-) , 男, 讲师。E-mail : cfy307@ sohu. com * 通讯作者(Corresponding author) : 陈亮( 1963-) , 男, 教授, 博士生导师, 研究方向: 细胞与分子生物学。E-mail: chenlg@ xmu. edu. cn[中文文摘]运用双向电泳分析高抗水稻品种“佳辐占”受强毒力细菌性条斑病病原菌侵染2d后的叶片蛋白质组变化,共发现38个蛋白质发生差异表达,其中32个上调,5个下调,1个新增。用MALDI-TOF-MS分析和数据库检索鉴定出其中的33个差异表达蛋白质,并将它们分为4个功能类群,即信号转导相关蛋白、防卫相关蛋白、代谢相关蛋白和蛋白质稳定相关蛋白。这些蛋白分别参与了信号识别、信号传递、抗氧化、糖代谢、细胞壁加固、植保素合成等抗病生理反应。研究表明,水稻对细菌性条斑病病原菌的侵染存在着一个复杂的抗病信号应答和代谢调控网络,其作用机理可以通过差异表达的蛋白质(酶)反映出来,其中差异表达的8个R蛋白和3个PR蛋白可能与水稻对细菌性条斑病的抗病性密切相关。本研究为进一步揭示水稻对细菌性条斑病的抗性机理及相关抗病基因的功能克隆提供了依据。[英文文摘]Rice bacterial leaf streak( BLS) caused by the pathogen Xanthomonas oryzae pv. oryzicola ( Xooc) is one of the major rice diseases in South China. Here we focus on proteomics as a tool for the discovery of differentially expressed proteins closely related to the disease resistance. The leaves of rice cultivar Jiafuzhan (Oryzae sativa L. ) highly resistant to the disease, were infected by"89773-1- 1" strain of the Xooc with strong pathogenicity. Total proteins were extracted from the leaves sampled at two days after inoculation, and separated by two- dimensional electrophoresis. It was found that there were thirty- eight proteins expressed differentially, of which thirty-two were up-regulated, five down-regulated and one was "new". Of the thirty- eight responsive proteins, thirty-three were identified by MALD-I TOF-MS and database searching.Based on the predicted function, we grouped them into four clusters: signal transduction, defensive responses, substance metabolism and protein stabilization, which were involved in many resistant physiological react ions, including signal recognition and transduction, antioxidant react ion, carbonhydrate metabolism, cel-l wall reinforcement and phytoalexin biosythesis. In turn a complex signal transduct ion and metabolic regulative network in the resistant responses to the infection of Xooc was outlined in this work, and the molecular mechanism was revealed by differentially expressed protein/enzyme patterns during Xooc infection. In this study, eight R proteins and three pathogenesis- related(PR) proteins which might relate closely to the disease-resistance were found. This result provides us the basic information to further reveal the resistant mechanism and conduct functional cloning of the resistan-t related genes in rice to BLS.生物农药与化学生物学教育部重点实验室( 福建农林大学) 开放课题基金项目( KF0411