Genome-wide DNA Methylation status in Neonates affected by Maternal Organochlorine Pesticides Exposure during Pregnancy

研究背景 双对氯苯基三氯乙烷（2，2-bis(chlorophenyl)-1，1，1-trichloroethane，DDTs）是在生产生活中最常见的有机氯农药之一（Organochlorinepesticides，OCPs），其半衰期较长，是一类可以通过食物链传递从而蓄积在脂肪组织、而且具有生物放大效应的有机化合物。而产前母亲与胎儿时刻承受着环境污染物暴露的风险。有的感染，特别是早期感染，可能直接导致怀孕失败与流产，或者造成胎儿畸形。关键问题是，对于大部分没有导致流产与畸形的感染，它们对孩子出生后的一生的疾病（比如免疫疾病）风险会产生重要的影响，而这些影响可能会通过表观遗传学（比如甲基化）...Background 2, 2 bis chlorophenyl-1,1,1-trichloroethane(DDTs) is a kind of common Organochlorine pesticides(OCPs) around environment, and it has a long half-life, which can accumulate in adipose tissue and has biological amplification effect. It is a critical period for the development of fetus during pregnancy, and the epigenome is particularly vulnerable to environmental pollutants in early st...学位：医学硕士院系专业：公共卫生学院_流行病与卫生统计学学号：3262014115054

Nutrition KAP survey on different occupational populations and its effect on disease prevention

随着我国经济的快速发展,人们生活方式、膳食模式和疾病谱发生了巨大变化,营养不平衡和慢性非传染性疾病已成为我国主要的公共卫生问题。其中,不同职业与营养相关性疾病一直是营养学界关注的问题之一。通过对不同职业人群营养知识、态度、行为的调查,了解各职业人群营养知识、态度、行为及其影响因素,及时采取有效措施如营养干预、健康教育等,提高各职业人群营养素养,改善其营养状况,对于营养相关疾病的防制有一定作用。作者主要介绍了近年来关于不同职业人群营养状况研究及营养知识态度行为调查分析,为职业人群营养相关疾病的防制提供参考依据。With the rapid development of the economy in our country,the life styles,dietary patterns and disease spectrum have changed vastly, while the nutritional imbalance and chronic non-communicable diseases have become major public health problems in China.Among them,the relationship between occupation and nutrition-related diseases has always been one of the severe problems in nutrition science.Through the nutrition knowledge-attitude-behavior(KAP)survey among different occupational populations,to understand the nutrition KAP of different occupational populations and its influencing factors,carry out the effective measures such as nutrition intervention and health education,enhance the nutritive condition of different occupational groups,and improve their nutritional quality have some effect to prevent the nutrition-related diseases.The authors mainly introduce the researches on nutritional conditions and investigation on nutrition KAP of different occupational populations in the recent years,and provide the reference for prevention and control of nutrition-related diseases among occupational populations.福建省卫生厅青年科研基金(项目编号:2012-2-100

Study on the risk factors for intrauterine infection of hepatitis B virus among pregnant women

目的探讨厦门市孕妇乙型肝炎病毒(hepatitis B virus,HBV)感染情况和HBV宫内感染的影响因素。方法在1; 064名孕妇第一次到医院建立孕产妇保健卡时进行问卷调查并检测其血清乙肝标志物,根据检测结果选择外周血乙型肝炎表面抗原(hepatitis B; surface antigen,HBsAg)阳性的产妇179例随访至其分娩,并采集其分娩时的脐血和胎盘组织。对脐血进行HBsAg和HBV; DNA的检测,对胎盘组织进行HBsAg的检测。采用chi~2检验和非条件Logistic回归分析相关因素与HBV宫内感染的关系。结果被调查的1; 064名孕妇中,HBsAg阳性的孕妇共179名,感染率为16.8%。对179名HBsAg阳性的孕妇的脐血进行检测发现,发生HBV宫内感染的孕妇共; 34名,感染率为19.0%。孕妇HBV宫内感染的危险因素为胎盘HBsAg阳性、孕妇血清乙型肝炎E抗原(hepatitis B e; antigen,HBeAg)阳性、孕前超重,OR分别为5.123(95% CI:1.422 ~ 18.413)、4.619(95%; CI:1.225 ~ 17.534)、 3.343(95% CI:1.233 ~; 9.092)。结论对于HBsAg和HBeAg双阳性的超重/肥胖孕妇,其新生儿可能发生HBV宫内感染,应加强自身防护措施,合理规避HBV宫内感染风; 险。Objective To investigate the prevalence of hepatitis B virus (HBV); infection in pregnant women and the risk factors for Intrauterine; infection of HBV among pregnant women in Xiamen. Methods Total 1 064; pregnant women were included in this study. A questionnaire was; performed to collect the general information of the subjects and HBV; infection status was tested on the first day when they came to the; hospital. Then HBsAg positive pregnant women were selected and followed; until delivery. We collected their cord blood and placenta tissue when; they delivery. HBsAg and HBV DNA in cord blood and HBsAg in placental; tissue were detected. Chi-square test and the non-conditional Logistic; regression analysis were conducted to investigate the correlation; between HBV intrauterine infection and risk factors. Results 179 out of; the 1 064 pregnant women were HBsAg positive. The infection rate was; 16.8%. 34 out of 179 HBsAg positive pregnant women were found; intrauterine transmission with a rate of 19.0%. The risk factors for the; occurrence of HBV intrauterine transmission were placenta HBsAg positive; (OR = 5.123, 95% CI:1.422-18.413),mothers with hepatitis B e; antigen(HBeAg) positive (OR = 4.619, 95% CI:1.225-17.534) and; overweight(OR = 3.343, 95% CI:1.233-9.092), respectively. Conclusions; Placenta HBsAg positive,mothers with HBeAg positive and overweight are; independent risk factors for the occurrence of HBV intrauterine; transmission.国家自然科学基金; 厦门市科技计划项

厦门市某医院体检人群中有机氯农药暴露与胆石症发病的关联性研究

目的探讨厦门市第二医院体检人群中有机氯农药暴露与胆结石发生的关联及其发病危险因素。方法收集2015年2月-8月在医院健康体检人群中经B超确诊的新发胆囊结石患者100例,以同期参加体检的非胆囊结石者为对照,进行1∶2配比的病例对照研究。进行流行病学调查,采用气相色谱联用电子捕获器检测研究对象外周血中有机氯农药水平。应用多因素条件Logistic回归模型分析有机氯农药与胆囊结石发病的关系。结果病例组血清中α-HCH残留水平高于对照组,差异有统计学意义;Logistic回归分析非酒精性脂肪肝、2型糖尿病、血清α-HCH含量增高可能增加胆结石的患病危险,OR值及95%CI分别为5.134(1.952~13.502)、4.754(1.849~12.220)和1.357(1.094~1.854)。结论环境有机氯农药暴露可能是胆囊结石病发生的危险因素。厦门市科技计划项目(3502Z20163006)；厦门市集美区科技计划项目(20142C01)；厦门大学校长基金(20720152012)

Optimization of separation methods and culture system of chicken embryonic stem cells in vitro

背景:胚胎干细胞是从动物早期胚胎的内细胞团或原始生殖细胞分离出来的具有发育全能性的一种未分化的无限增殖细胞系。而鸡胚胎干细胞则是从X期鸡胚的胚盘分离而来。目的:优化鸡胚胎干细胞分离方法和离体培养体系。方法:采用滤纸纸环-发环的方法从X期鸡胚分离胚盘细胞,并采用STO细胞作为饲养层和大鼠肝细胞(brl)条件培养基(CM)+细胞因子作为离体培养体系对分离的胚盘细胞进行培养。结果与结论:滤纸纸环-发环法获得的完整胚盘率为75%~85%,克隆形成率约为50%。brl-CM+饲养层培养体系,鸡胚胎干细胞可传至7代,而brl-CM+饲养层+细胞因子培养体系,鸡胚胎干细胞可传至25代。分离到的鸡胚胎干细胞,经碱性磷酸酶染色、SSEA-1染色鉴定,表明鸡胚胎干细胞处于未分化状态。提示,实验不仅优化了鸡胚胎的分离方法,获得完整且杂质少的胚盘,而且进一步优化了鸡胚胎干细胞体外培养体系。BACKGROUND:Embryonic stem cells are undifferentiated permanent cell line derived from inner cell mass cells and primordial germ cells of animal's early embryos.Chicken embryonic stem cells are derived from the blastodermal of a X-stage embryo.OBJECTIVE:To optim the separation method and in vitro cultural system of chicken embryonic stem cells.METHODS:The X-stage chicken embryos were isolated by using a small square of ?lter paper with a hole punched in the center,and the blastodermal cells were isolated by using the hair loop.STO cells were used to make feeder layer;at the same time,BRL-CM and cytokine were also used for chicken embryonic stem cells in vitro cultural system.RESULTS AND CONCLUSION:The filter paper loop and the hair loop could obtain complete the blastoderm,and the successful percentage was 75%-85%.The colony formation rate was about 50%.After culture in the BRL-CM + feeder layer + cytokine culture system,the passage of CES cells is the seventh generation;BRL-CM + feeder layer + cytokines,cultured chicken embryonic stem cells could passage to the 25th generation.Isolated chicken embryonic stem cells were in an undifferentiated state detected by alkaline phosphatase staining and SSEA-1 staining.The findings indicate that this experiment not only optimized the isolation method of chicken embryonic stem cells to obtain complete and pure embryos,but also further improved the in vitro culture system of chicken embryonic stem cells.国家973项目(2009CB941600)资助;国家自然科学基金项目(31072101)资助---

产前滴滴涕或多氯联苯暴露与出生缺陷风险相关性的Meta分析

目的系统评价孕妇产前暴露于滴滴涕(DDT)或多氯联苯(PCBs)与新生儿出生缺陷风险之间的关系。方法计算机检索PubMed、EMbase、WanFang Data、VIP和CNKI数据库,搜集有关产前DDT或PCBs暴露与出生缺陷关系的病例-对照研究,检索时限均从建库至2017年2月。由2名研究者独立筛选文献、提取资料并评价纳入研究的偏倚风险后,采用Stata 13.0软件进行Meta分析。结果共纳入14个研究,包括2 238例出生缺陷新生儿和2 355例对照新生儿。Meta分析结果显示:产前暴露于高水平的DDT会导致新生儿隐睾发生率增加[OR=1.12,95%CI(1.09,1.15),P<0.001]。而DDT暴露与尿道下裂和神经管畸形发生没有相关性。产前暴露于高水平的PCBs与隐睾、尿道下裂、神经管畸形发生也没有相关性。结论孕妇产前暴露于高水平的DDT可能是隐睾发生的危险因素。受纳入研究数量和质量的限制,上述结论尚待更多高质量研究予以验证。厦门市科技计划项目(编号:3502Z20163006);;厦门市集美区科技计划项目(编号:20142C01

Combustion Synthesis and Electrochemical Properties of the Small Hydrofullerene C50H10

通讯作者地址: Xie, SY (通讯作者),Xiamen Univ, Coll Chem & Chem Engn, State Key Lab Phys Chem Solid Surfaces, Xiamen 361005, Peoples R China 地址: 1. Xiamen Univ, Coll Chem & Chem Engn, State Key Lab Phys Chem Solid Surfaces, Xiamen 361005, Peoples R China 2. Xiamen Univ, Coll Chem & Chem Engn, Dept Chem, Xiamen 361005, Peoples R China 3. Xiamen Univ, Sch Life Sci, Xiamen 361005, Peoples R China 电子邮件地址: [email protected]; [email protected] hydrofullerene C50H10 is synthesized by low-pressure benzeneoxygen diffusion combustion. The structure of C50H10 is identified through NMR, mass spectrometry, and IR and Raman spectroscopy as a D5h symmetric closed-cage molecule with five pairs of fused pentagons stabilized by ten hydrogen atoms. UV/Vis and fluorescence spectrometric analyses disclose its optical properties as comparable with those of its chloride cousin (C50Cl10). Cyclic and square-wave voltammograms reveal that the first reduction potential of C50H10 is more negative than that of C50Cl10 as well as C60, with implications for the utilization of C50H10 as a promising electron acceptor for photovoltaic applications.973 projects 2011CB935901 NSFC 21031004 21021061 2077310

Battery-aware Bi-directional Search Routing Protocol for Wireless Sensor Network

在无线传感器网络(WSn)中,当有数据流传输时,一些节点会因网络能量分布不均而过早失效,使得源节点不得不通过另一条更长且低效的路径才能将数据包路由至目的节点,从而影响网络寿命.为了延长网络的生存时间,需要设计能量有效的协议,适应传感器网络的特点.提出了一种电池感知的双向搜索路由(bATTEry-AWArE bI-dIrECTIOnAl SEArCHrOuTIng PrOTOCOl,bAr-bIdS)协议,该协议通过改进深度优先搜索,能在网络中已有部分节点因能量耗尽而失效的情况下,找到一条跳数相对较少的路径,从而减少网络中的能量消耗.且该协议能较好感知节点的电池状态,减少节点电池的放电损耗.仿真结果表明,当网络中有数据流传输时,bAr-bIdS协议能较好均衡全网节点的能量消耗、延长网络存活时间.When there has streaming data transmission in the wireless sensor networks(WSN),some nodes might die owing to exhausting their energy,which makes the source node has to route data packets to the destination node through a longer but inefficient path and furtherly influences the lifetime of the network.In order to prolong the network life span,energy-efficient protocols should be designed to adapt the characteristic of WSN.We propose a battery-aware bi-directional search routing(BAR-BIDS) protocol.By making better the depth first search algorithm,the network can find a path with less hops to route the data packet in the case of many nodes are failure because of energy depletion.Moreover this protocol can obtain the battery status well and decrease the discharging loss.The simulation results prove the good performance of BAR-BIDS protocol in balancing the energy consumption and prolonging the lifetime of the whole network,when there has streaming data transmission in the network.福建省重大科技专项(2007HZ0003);福建省重点科技项目(2008H0037

基于MMOGA的某超高速包装机推手机构动平衡优化

为了改进某型超高速包装机推手机构的动态性能,对该机构进行动平衡优化。基于多刚体动力学理论,建立该推手机构的动力学模型,分析出主要产生惯性载荷的零部件。建立该部件的数学模型,推导出摆动力和摆动力矩方程。基于微型多目标遗传算法（MMOGA）,综合考虑摆动力和摆动力矩的均方根值,以杆件的质量、质心位置等为变量,对该部件进行动平衡优化。根据变量的较优值,重新设计长臂推手的结构。优化后推手机构的摆动力与摆动力矩分别减小了23. 6%和29. 5%,且长臂推手的固有频率远离了260 Hz（激振频率）。上述结果表明,经过优化,该型包装机推手机构的动力学性能得到了较明显的改善,避免了长臂推手的共振现象

TiO2空心球多聚体光催化剂及其制备方法和应用

本发明公开了一种TiO2空心球多聚体光催化剂及其制备方法和应用。本发明采用直接腐蚀钛片的方法制备TiO2空心球多聚体：将预处理后的钛片置于氟化铵澄清水溶液中进行水热反应，待反应溶液冷却后，收集反应溶液中的沉淀物，洗涤沉淀物，烘干，煅烧，即得。该制备方法简单，制备时间短。制备得到的TiO2空心球多聚体光催化剂具有很好的紫外光响应、量子效率高，能有效地催化降解挥发性有机物