Study on Remote Monitoring System for Fish Activity and Water Quality Environment in Offshore Fish Cage

在全球海洋渔业资源日益衰减的今天，深水网箱以其抗风浪、耐海流能力强、养殖容量大、高产高效、环保等优势成为现代耕海牧渔的发展方向。深水网箱养殖过程中，由于网箱离岸远，范围广，水体浑浊，不容易观察鱼群活动状态，同时也往往难以把握合适的投饲量，投饵过少将影响鱼类生长健康，过多则增大养殖饲料成本，同时剩余饵料又会污染环境。为了进行鱼群状态监测和避免饲养投喂的随意性和盲目性，急需加强深水网箱鱼群状态与水质环境监测，为正确判断鱼群食欲、科学进行投饲提供重要参考依据。本文根据深水网箱养殖环境的特殊要求，设计并实现了一套结构简单、功耗低、体积小、远程、实时的深水网箱鱼群状态与水质环境监测系统。 主要内容： ...The global marine fishery resources are going downhill nowadays. Fish culturing in offshore fish cage is being the developing trend of marine aquaculture for its advantages of great resistance to wind, waves and strong-flowing currents, large breeding capacity, high yield and high efficiency. However, it is difficult to grasp the appropriate amount of feeding, feeding little would affect the growt...学位：理学硕士院系专业：海洋与地球学院_海洋物理学号：2232013115144

Establishment of MDCK cell lines which stably express visualable human neonatal Fc receptor

[目的]建立稳定表达融合EGFP的人新生儿Fc受体（h FcRn）的MDCK细胞株。[方法]构建重组慢病毒质粒p EGFP-h FcRn,采用四质粒包装系统共转染HEK 293T细胞生产重组慢病毒,感染MDCK细胞后对EGFP阳性细胞进行流式单细胞分选;通过Western Blot及EGFP-β2m荧光共定位验证h FcRn的完整性,并用流式细胞仪检测h FcRn与人Ig G的结合活性。[结果]测序结果表明成功构建p EGFP-FcRn慢病毒表达载体;感染后EGFP阳性MDCK细胞比例约为26.5%,流式单细胞分选后得到纯阳性细胞;荧光共定位及Western Blot均检测到h FcRn的完整表达;流式分析表明细胞株上的h FcRn与Ig G存在p H依赖性结合。[结论]成功获得稳定表达具有生物活性的可视化h FcRn的MDCK细胞株。[ Objective] To establish MDCK cell line stably expressing EGFP- human neonatal Fc receptor（hFcRn） fusion protein. [ Methods ] The lentiviral expression vector for EGFP - hFcRn fusion protein was constructed. Generating by co - transfection of four -plasmids into HEK 293T cells ,the lentivirus particles were used to infect MDCK cell line. EGFP positive single cell was obtained by FACS, and then FcRn expression was identified by fluorescence co -location with EGFP - β2m and confirmed by Western Blot. Flow cytometry was used to detect binding activity of hFcRn and human IgG. [ Results ] DNA se- quencing demonstrated that the lentivirus vector pEGFP - FcRn was constructed successfully. The percentage of EGFP - posi- tive ceils was about 26.5% after infection. Expression of the complete protein was detected through fluorescence co - location and Western Blot, respectively. Flow cytometry analysis showed that the cell lines could pH - dependently capture human IgG. [ Conclusion] MDCK cell line stably expressing functional visualable hFcRn was established.基金项目：国家自然科学基金项目（“结构生物学指导的HBV治疗性抗体人源化及其关键技术研究”,No.31600748;“抗呼吸道合胞病毒高中和活性抗体的保护机制研究”,No.81401668;“基于广谱中和单抗的通用型流感疫苗设计及其结构基础研究”,No.31670934

Environment Security Monitoring System for Offshore Caged Fish and Water Quality

为了更好地开展渔业养殖中深水网箱中鱼群状态与水质环境的安全监测,提出了一种深水网箱鱼群与水质环境安全远程监测系统.该系统使用声学方式对鱼群分布状况进行监测;同时采用传感器对水质环境的温度、溶解氧、p H值等进行监测,监测结果通过无线数据传输方式实时传送至微处理器,完成信号去噪、数据提取、显示及报警等.实验结果表明,该监测系统性能稳定可靠,结合嵌入式控制技术与无线数据传输技术,监测操作简便,移动性好;同时兼顾鱼群与水质环境安全,监测更全面到位,在推广深水网箱养殖安全配套设施上具有良好的应用前景.Environment monitoring of fish activity state and water quality is the key to offshore cage safety management.A remote monitoring system for offshore caged fish and water quality is developed in the research.The fish distribution and abundance are monitored strategically using acoustic transducers in the system.Meanwhile,some parameters of water quality are obtained using various sensors,such as temperature sensor,dissolved oxygen sensor and p H sensor. Then the collected data are sent to the user monitor terminal in real time through wireless transmission module.Finally,the results are displayed on a 7-inch liquid crystal display screen( LCD) after data processing. The experiment has been conducted to verify the excellent performance of this system.Combined with embedded control technology and wireless data transmission technology,the flexibility and mobility are improved significantly. Besides,both fish activity and water quality are taken into account,making the monitoring more comprehensive.The results suggest that the system has good application prospects.国家海洋局海洋公益性行业科研专项(201505027-2

On Teaching Reform and Practice at Biochemical Engineering Experiments

本文从工科专业实验课教学的重要性入手,阐述了进行生物工程专业实验课教学改革的必要性和重要意义,介绍了生物工程实验教学改革的相关体会。This article,based on the importance of the engineering experiments teaching,expounds the necessity and importance of the reform in Biochemical Engineering Experiments Teaching,and also introduces the experience about Biochemical Engineering Experiments Teaching reform

Polyphenol Contents in Eight Fruits and Their Antioxidant Activities

[中文摘要]测定了苹果、石榴、橄榄、芒果、香蕉、菠萝、葡萄和龙眼的壳、肉及核中的多酚含量及其抗氧化性。以70%丙酮(v/v)为提取溶剂,室温下超声波辅助浸提样品后得到提取液。采用普鲁士兰(Prussian blue)法测定了提取液中多酚和单宁的含量,利用FRAP法测定其抗氧化性。结果表明:提取液中多酚和单宁的含量均与其抗氧化性成正相关关系;石榴、橄榄、芒果、葡萄和龙眼等水果的壳与核有望成为天然抗氧化剂的新来源。[英文摘要]The contents o f polyphenols of peel pulp and seed fractions of apple, pome granate, olive, mango, banana, pineapple, grape and long an and their to talantioxidant ac tiv ities were studied. A fter ultrasound-assisted extraction w ith 70% aqueous acetone at room temperature, polypheno ls and tann ins in the p lant ce lls w ere separated and their contents were determ ined by Prussian blue assay. Antioxidant activities of the aqueous acetone extracts were evaluated by Ferric Reducing /Antioxidant Power (FRAP) assay. A positive linear correlation between antioxidant activities and phenolic contents was observed. The resu lts show ed that the fru it pee l and seeds o f pom egranate, olive, mango, grape and longan could be conside red as potentia l sources o f antioxidants rather than just discarded as waste

基于多聚化重组抗原的检测戊型肝炎病毒IgM与IgG抗体的ELISA的建立及初步评估

建立并评估分别检测戊型肝炎 (戊肝 )病毒IgM与IgG抗体的捕获法及间接法ELISA。以原核表达的多聚化重组HEV蛋白为抗原 ,建立戊肝捕获法IgMELISA(E2 -IgM )和间接法IgGELISA(E2 -IgG)。利用 2 9只实验感染猴系列血清及多份临床急性肝炎血清、正常人血清以及单克隆抗体评估所建立的方法的敏感性与特异性 ,并与商品化试剂 (Genelabs公司抗 -HEVIgG和IgM试剂 ,GL -IgG/GL -IgM )进行比较。 2 9只恒河猴E2 -IgM和E2-IgG的阳转率均为 10 0 % ,其中 75 %在感染后 4周内阳转 ,均早于ALT异常时间。E2 -IgM持续 2～ 14周 ,平均6周 ;E2 -IgG在 70周时仍无一阴转。GL -IgG阳转率为 79 3% ( 2 3/ 2 9) ,多数晚于ALT异常时间 ,平均持续约18周 ,但最长为 1只在感染后 70周时仍为阳性。用E2 -IgM试剂盒检测 92 8份正常人血清 ,仅 2份OD值略高于0 2。检测 5 10份临床急性肝炎血清 ,可明显将其区分为 2个部分 ,一个部分OD值小于 0 2 ,其OD值分布与正常人相似 ;另一个部分OD值大于 0 4,共 131份 ,其中 10 9份大于 1 0。可能分别对应于急性肝炎中的非戊肝患者和戊肝患者。 119份非甲～丙急性肝炎中 ,E2 -IgM阳性 5 7份 ,GL -IgM阳性 2 9份 (E2 -IgM均阳性 )。 5 0 6 0份普通人群血清的E2 -IgGOD值在 0 2以下 ,形成一个

戊型肝炎诊断中血清学及核酸检测意义的研究

目的 利用恒河猴感染模型评估血清学检测和核酸检测在戊型肝炎诊断中的临床意义。 方法对86只戊型肝炎病毒(HEV)实验感染猴的系列血清和粪便标本,用反转录聚合酶链反应方法进行病毒学检测,用4种酶联免疫吸附抗体检测试剂(E2-IgM,E2-IgG,GL-IgG和YES-IgG)进行血清学检测。 结果 感染猴均产生E2-IgG抗体,除1只感染猴未检出粪便排毒和E2—IgM外,其余感染猴均出现粪便排毒和E2-IgM阳转。GL-IgG和YES-IgG的阳转率较低,而且与感染剂量相关。急性肝炎主要开始于感染后3～7周内。病毒学指标在潜伏早期即已出现,较疾病的发生约早2周,并在急性期迅速下降。E2-IgM在发病时已有约2/3阳转,并在发病后10周内完全转阴。E2—IgG几乎与E2—IgM同时阳转,在所有感染猴中持续存在,直至86周后仍无阴转。GL-IgG和YES-IgG抗体较E2抗体晚约1周,在感染后20周内已有过半数转阴。结论 E2-IgM是一个良好的戊型肝炎急性感染诊断指标,E2-IgG是一个良好的戊型肝炎既往感染诊断指标,GL-IgG和YES-IgG抗体的阳转或双份血清滴度升高可以作为辅助诊断指标,病毒核酸检测仅在发病的极早期具有诊断价值