Monoclonal antibodies against HPV11 virus-like particles: functional characteristics and application on quality assessment

目的分析和鉴定抗HPV11病毒样颗粒(virus-like particle,VLP)鼠源单克隆抗体的性质,筛选性质和生物学活性较优的抗体,并初步应用于抗原或疫苗的质量分析。方法分别利用间接ELISA法和Western blot对HPV11的22株单克隆抗体的亚类、与HPV11 VLP的结合能力和构象敏感性进行检测;采用血凝抑制实验对单克隆抗体的血凝抑制活性进行分析;运用基于假病毒的抗体中和实验对单克隆抗体的中和活性进行鉴定,选出中和活性高的单抗进行两两配对,采用双抗夹心ELISA法捕获单抗并筛选合适的配对双抗。结果对22株单抗的性质进行了详细和完整的鉴定,并根据构象敏感性进行排序,筛选出6株型别特异、结合活性强且中和活性高的单抗(2A2、4A1-3、16G7、14A6、9C12和19C7);成功建立了基于单抗的双抗夹心(14A6∶Ag∶9C12-HRP)ELISA定量分析方法。结论获得了较全面的HPV11 VLP单抗性质信息,建立了重组HPV11抗原质量分析的双抗夹心ELISA法,为HPV11抗原的生命周期管理或尖锐湿疣疫苗的研发、工艺优化、产品放行和稳定性研究等提供了技术支持。Objective To quantitatively analyze the characteristics of a panel of murine anti-human papillomavirus( HPV) 11 L1-derived virus-like particle( VLP) monoclonal antibodies( m Abs) and establish the m Ab-based methods for antigen quality analysis. Methods A panel of 22 murine anti-HPV11 m Abs were characterized in details with their isotype,and binding affinity,conformational sensitivity were examined quantitatively in the direct binding ELISA and Western blot. The hemagglutination inhibition activity of m Abs were identified using the hemagglutination inhibition assay and the pseudovirus( Ps V) neutralization efficiency were examined quantitatively using the Ps V-based neutralization assay. The type-specific, highly conformational sensitive and neutralizing m Abs were selected to be used in the sandwich ELISA assay. Results Based on the quantitative and semi-quantitative results,six type-specific,highly conformational sensitive and neutralizing m Abs( 2A2,4A1-3,16G7,14A6,9C1 and 19C7) were identified. These m Abs,along with 10D6 were screened as the capture m Ab or as the detection m Ab in the sandwich ELISA. Conclusion The binding affinity,conformational sensitivity and neutralization efficiency of anti-HPV11 m Abs were characterized in details. A m Ab-based sandwich ELISA assay( 14A6∶Ag∶9C12-HRP) were developed,which could be used in the in vitro potency analysis of HPV11 VLP-based vaccine.重大新药创制(2015ZX09101034

关于“经济发展方式转变进程中的财税政策”的探讨(笔谈)

转变经济发展方式是当前中国的热门话题,而上海在城市发展中又肩负着率先转变的重要任务,在这一意义和背景下,2010年4月24日由上海金融学院学院主办、上海金融学院公共经济管理学院承办的“《中国城市财政发展报告2009/2010:促进‘两个中心’建设的上海城市财政》首发式暨‘经济发展方式转变进程中的财税政策’论坛“在我校举行。与会专家围绕经济发展方式转变进程中的财税政策的“趋势与目标“、“机遇和挑战“、“实施方略“和“地方实践“等四个专题进行研讨,各抒己见,智慧交锋。现将其真知灼见整理摘要与读者分享,以期待更多学者共同关注当前经济发展方式转变进程中的财税政策

On the Problem of the Stakeholders Adopting Close Management in Mount Wuyi

武夷山封闭式管理引发了利益相关者的利益重构问题。本文通过对武夷山封闭式管理中各利益相关者的实地调研和走访,以及其后开展的跟踪研究,探讨了各利益相关者的利益分歧点及其相互关系,提出只有顾全利益相关者,保证多方利益的协调,建立利益均衡的景区合作管理模式,才是景区封闭式管理困境的最佳破解途径。The close management in Mount Wuyi has caused stakeholder problems. Through on-the-spot investigation in Mount Wuyi and subsequent tracking study, this paper discusses the interest bifurcation of the stakeholders and the relationship among them, and regards that the best solution to the problem is to build an interest-equilibrium and cooperating management model concerning about all the stakeholders and dealing with interest relationships

用高速阴影技术研究K9玻璃中的失效波

用高速阴影摄影技术研究了爆轰加载下K9玻璃样品中波的传播和压缩区内损伤破坏的物理图象和规律。实验中观测到冲击波阵面后有一个移动速度为2.1mm/μs～2.2mm/μs的黑色阴影区边界，即失效波(Failure wave)；实验发现只有当冲击载荷接近材料的HEL时，在冲击波 和失效波之间的区域才有少量的微裂纹成核和长大，而在冲击载荷较低时却没有观察到；同时实验中观测到失效波萌生于被撞击面，并在两块玻璃的交界面上观测到失效波的再生。这些结果表明失效波的产生基本与冲击相变无关，主要与玻璃样品表面的初始损伤有关

用高速阴影技术研究K9玻璃中的失效波=Investigations of Failure Waves in K9 Glass Using Shadowgraph

用高速阴影摄影技术研究了爆轰加载下K9 玻璃样品中波的传播和压缩区内损伤破坏的物理图象 和规律。实验中观测到冲击波阵面后有一个移动速度为2. 1～2. 2 mm/μs 的黑色阴影区边界,即失效波(Failure wave) ;实验发现只有当冲击载荷接近材料的HEL 时,在冲击波和失效波之间的区域才有少量的微裂纹成 核和长大,而在冲击载荷较低时却没有观察到;同时实验中观测到失效波萌生于被撞击面,并在两块玻璃的交 界面上观测到失效波的再生。这些结果表明失效波的产生基本与冲击相变无关,主要与玻璃样品表面的初始 损伤有关,换言之,失效波是玻璃样品表面微裂纹在冲击波作用下失稳扩展造成的