靶向人类免疫缺陷病毒1型(HIV-1)整合酶多肽药物表达载体的构建

目的构建人类免疫缺陷病毒1型(HIV-1)整合酶(IN)表达载体用于双分子荧光互补技术筛选多肽药物。方法PCR扩增HIV-1 IN的全长基因序列,将目的片段插入至pBiFc-VN173载体中,并对重组质粒pBiFc-VN173-IN进行双酶切及测序鉴定。将重组质粒pBiFc-VN173-IN和对照组质粒pBiFc-VN173分别转染HEK293T细胞,采用Western blot法和免疫荧光细胞化学染色法检测IN的表达。结果通过高保真PCR、载体构建和鉴定,成功获得IN表达载体pBiFc-VN173-IN。与对照组相比,通过Western blot法和免疫荧光细胞化学染色法证实转染质粒pBiFc-VN173-IN载体的HEK293T细胞表达IN。结论成功构建了IN表达载体,该载体可用于和IN相互作用的多肽药物的双分子荧光互补技术筛选。福建省教育厅中青年教师教育科研项目(JAT171033

A study on the function and mechanism of neural guidance molecular receptor UNC5C in developmental vascular system

血管新生（Angiogenesis）是指在已有的血管的基础上生长和形成新血管的过程。充分了解血管新生的调控机制不但具有重要的生物学意义，也有助于血管增生和缺陷等疾病的治疗。在血管发育机制中，血管的布线（wiring）及导向控制机制（guidingmechanism）的发现是我们从整体的水平上认识血管系统发育的重要进展。脊椎动物的神经系统和血管系统不仅解剖学形态相似，而且共用相似的导向因子。已有报道表明，4个主要神经导向因子家族中都有成员参与了血管新生的调控过程，但是这些报道存在很大争议，而且还可能存在未知的神经导向因子调节血管新生。 本实验室的前期研究工作表明，在鸡的血管内皮前体细胞和3种人...Angiogenesis is an essential process for growth of the blood vasculature, especially formation of new vessels from existing ones. Understanding the regulation of angiogenesis is crutial in clinic practice to solve the most perplexing problems arised from the abnormal growth of blood vessels. The discovery of wiring and guiding mechanisms in the vascular development provide not only new angle of vi...学位：理学硕士院系专业：生命科学学院_细胞生物学学号：2162013115255

家庭承包制下土地流转的农户解释:对重庆不同经济类型区七个村的调查分析

利用大量农访数据,检查了家庭承包制度下农业土地利用的重要方面,测算了样点村现有土地流转状况及其农户解释。结果表明:流转规模,总体呈渝西经济走廊快于都市发达圈,更快于三峡库区生态经济圈;流转模式,转包已逐渐被农民所接受和采用,而以现金兑换的出租和转让方式,也渐渐被部分农民所喜欢;流转用途,都市发达圈土地流转后土地利用主要向非农用转变,而渝西经济走廊和三峡库区生态经济圈除小部分向非农用转变外,大部分土地流转后提高了土地利用的集约度。土地权属在总量上制约着土地流转的规模,但这一作用的区域差异不明显;农村土地市场发育程度不仅影响区域土地流转的总体规模,而且是导致不同区域流转规模各异和流转方式演化差异的根本原因;社会保障的完善程度对农民是否愿意离土和采取何种方式离土具有决定性作用;土地流转管理包括权益保障管理和流转后土地用途管理,是规范土地流转主体行为,减少流转纠纷,保障土地流转依法展开的基础性力量。土地流转中,把握宏观经济环境变化背景下的农户心理及其对现有土地流转驱动因素的响应,对安排遵循市场规律和贯彻以人为本价值取向的土地流转制度意义重大

JUNO Sensitivity on Proton Decay p→νˉK+p\to \bar\nu K^+ Searches

The Jiangmen Underground Neutrino Observatory (JUNO) is a large liquid scintillator detector designed to explore many topics in fundamental physics. In this paper, the potential on searching for proton decay in $p\to \bar\nu K^+$ mode with JUNO is investigated.The kaon and its decay particles feature a clear three-fold coincidence signature that results in a high efficiency for identification. Moreover, the excellent energy resolution of JUNO permits to suppress the sizable background caused by other delayed signals. Based on these advantages, the detection efficiency for the proton decay via $p\to \bar\nu K^+$ is 36.9% with a background level of 0.2 events after 10 years of data taking. The estimated sensitivity based on 200 kton-years exposure is $9.6 \times 10^{33}$ years, competitive with the current best limits on the proton lifetime in this channel

Determination of the number of ψ(3686) events taken at BESIII

The number of ψ(3686) events collected by the BESIII detector during the 2021 run period is determined to be (2259.3±11.1)×106 by counting inclusive ψ(3686) hadronic events. The uncertainty is systematic and the statistical uncertainty is negligible. Meanwhile, the numbers of ψ(3686) events collected during the 2009 and 2012 run periods are updated to be (107.7±0.6)×106 and (345.4±2.6)×106, respectively. Both numbers are consistent with the previous measurements within one standard deviation. The total number of ψ(3686) events in the three data samples is (2712.4±14.3)×10^

JUNO sensitivity on proton decay p → ν K + searches*

The Jiangmen Underground Neutrino Observatory (JUNO) is a large liquid scintillator detector designed to explore many topics in fundamental physics. In this study, the potential of searching for proton decay in the $p\to \bar{\nu} K^+$ mode with JUNO is investigated. The kaon and its decay particles feature a clear three-fold coincidence signature that results in a high efficiency for identification. Moreover, the excellent energy resolution of JUNO permits suppression of the sizable background caused by other delayed signals. Based on these advantages, the detection efficiency for the proton decay via $p\to \bar{\nu} K^+$ is 36.9% ± 4.9% with a background level of $0.2\pm 0.05({\rm syst})\pm$$0.2({\rm stat})$ events after 10 years of data collection. The estimated sensitivity based on 200 kton-years of exposure is $9.6 \times 10^{33}$ years, which is competitive with the current best limits on the proton lifetime in this channel and complements the use of different detection technologies