HBV整合的致癌性研究

HBV的4个亚基因产物中,HBx具有反式激活、介导细胞凋亡作用,HBs具有反式激活因子的作用。肝细胞癌(HCC)的发生与HBV的整合及整合后染色体重排有关。为探讨HBV整合及HBx、HBs亚基因在HCC发生中的可能作用,我们制备了HBV亚基因探针,并以此对肝细胞癌中HBV整合及亚基因转录进行了研究,以期为阐明HBV感染后HCC发生机制提供一定的实验资料和依据。本研究中,首先以BamHⅠ、BglⅡ酶切扩增回收的HBVDNA ,回收其HBV亚基因HBx、HBc、HBs、PreSDNA片段,以地高辛甙元随机引物法标记成敏感性与特异性俱佳的HBV亚基因探针,再以HBV探针点杂交检测HCC标本中HBVDNA的阳性率,对阳性的标本,继以Southern转膜杂交检测其整合情况,最后取HBVDNA阳性的HCC标本,按经典方法抽提其mRNA ,分别以HBV亚基因探针Northern转膜杂交检测亚基因的表达率。结果显示:4 4例HCC标本中,75 %的标本显示有HBVDNA的存在;Southern杂交分析,HBV纯整合型HCC为6 3.6 % ,混合型HCC为36 .4 % ,未见单纯游离型HCC标本存在;17例HBVDNA阳性..

~(31)P NMR与HPLC联用筛选高效有机磷降解细菌

从有机磷农药污染地区分离到 14株具有高效甲胺磷降解活力的细菌 ,并用HPLC对其降解效率进行分析 ,发现一周内降解率最高达 74 % .采用3 1PNMR对其降解产物进行了同步监测 ,发现其降解产物不同 ,其中有三株可以把甲胺磷代谢成磷酸 .这三株降解细菌可以从环境中有效的清除有机磷的污

Research on Simulation System of 3D Orchid Flower Blossoming Process

我国的国花——兰花类属繁多,形态各异,目前尚未有人对其进行花开仿真研究。通过研究兰花植物学知识,以几种不同形态特征的兰花为虚拟建模对象,分析其器官形态特点,提出基于器官模型库的带随机值参数变换算法,建立器官模型库,并通过适当的变换,得到形态各异的兰花器官,根据兰花生长规律,采用不同的分段参数控制各器官生长,通过项目实践,证明了此方法能逼真地模拟兰花动态生长。同时加入了良好的交互功能,使用户能体验兰花的不同形态及在不同场景中摆放的效果。系统对7种兰花进行了模拟,在展会中通过该系统,销售额增加了10%。As the national flower of China, orchid has various kinds of types and shapes, and its bloom process has never been simulated before.A model library of orchid organs was established and a transformation algorithm with random-valued parameters was proposed based on the model library of orchid organs by studying botanical knowledge of orchids and taking orchids with different morphological characteristics as research subjects for virtual modeling.Different segmental transformations were respectively performed on each organ of orchids to simulate growth of the organs according to the growth law of orchids.Different examples for simulating different type of orchids were provided to illustrate the realistic of dynamic growth of orchid by using the transformation algorithm.Meanwhile, the positive user interactive function of the simulation system enabled users to experience different orchids in different scenarios.Seven species of orchids were simulated in the system, and sales volume of orchids increased by 10% at the exhibition by using the system.福建省中青年教师教育科研项目(JB14210); 福建省科技重大专项(2013HZ0004-1

用FTIR测定羧酰化壳聚糖和氰乙基壳聚糖的取代度

从壳聚糖 (脱乙酰度分别为 84%和 70 % )合成不同取代度的羧酰化壳聚糖和氰乙基壳聚糖作为标样 ,标样的取代度 (DS)由NMR或元素分析确定 .研究以FTIR作为工具测定这两个系列衍生物的取代度的方法 .吸光度用基线法得到 ,对所有不同探针谱带 ,参比谱带和基线作图法的组合进行比较 ,并通过 (A探针/A参比) /DS的平均相对偏差评价每种组合的优劣 .结果表明 ,对羧酰化壳聚糖 ,最合适的A探针/A参比 是A174 0 /A152 7;对氰乙基壳聚糖 ,最合适的A探针/A参比 是A2 2 4 9/A1590 .前者不仅适用于不同取代度的同一种O 羧酰化壳聚糖 (如丙酰化壳聚糖 )而且适用于不同取代度且不同碳数的 4个O 脂肪酸羧酰基取代的壳聚糖样品 .这两种组合的工作曲线的斜率分别为 2 .1(对A174 0BL1/A152 7BL1) ,0 .5 9(对A2 2 4 9/A1590BL1)和 0 .6 6 (对A2 2 4 9/A1590BL2 ) .实验还表明 ,不同的基线作法对结果影响甚

厦门市碘缺乏病防治现状与对策

[目的]了解碘缺乏病防治现状,探讨今后防治措施与策略。[方法]采用容量比例概率法抽查学生甲状腺大小、尿碘、盐碘,开展加工厂盐、居民户盐及盐民自用盐的盐碘含量监测。[结果]查2525名8～10岁学生,甲状腺肿大率为3.72%(触诊法),自1995年以来呈逐年下降趋势,合格碘盐食用率89.66%,碘盐覆盖率90.72%,尿碘中位数为246.40μg/L;加工厂盐合格率为99.15%,居民户碘盐覆盖率为95.25%、合格碘盐食用率为94.10%,盐民自供盐10月份后合格碘盐食用率已达到90%以上。[结论]我市继续保持在实现消除碘缺乏病阶段目标,但今后应坚持开展病情监测和坚持防制措施不松懈

Direct Fabrication of Ultrafine Electrospinning Nanofiber

采用装配有疏水铜网的新型喷头研究了超细纳米纤维的制备.静电纺丝实现之前,首先对铜网进行了疏水处理,并将其安装于喷头前端.静电纺丝过程中,聚合物溶液由精密注射泵输送至喷头处.安装于喷头的铜网可将管道内的聚合物溶液分成多股细流从铜网网孔中流出.从铜网网孔流出的溶液细流受电场力作用被拉伸成多股独立射流,并从喷头携带走聚集的正电荷.受铜网表面疏水性和射流间电荷排斥力的影响,从铜网喷射出的多股射流都将保持其独立的轨迹而不会产生聚集.疏水铜网有利于减小纺丝射流的初始直径,并获得均匀的超细纳米纤维.利用新型的电纺丝喷头成功制备了直径20~80 nM的聚氧化乙烯(PEO)和聚乙烯醇(PVA)超细纳米纤维.实验结果表明,超细纳米纤维的直径随着电纺丝溶液浓度的增加而变大.A novel spinneret assembled with Cu grid was presented in this paper to fabricate ultrafine nanofiber directly.Before electrospinning,hydrophobic treatment was performed on the Cu grid,which was then fixed at the front end of spinneret.During electrospinning,the polymer solution was transferred to the spinneret by the precise syringe pump.Through the holes in the Cu grid,polymer solution flow was divided into several smaller ones.The fine liquid flow from each hole of Cu grid was stretched into individual jets by the electric field force,and the liquid jets carried away the positive charges accumulated on the spinneret.Due to the hydrophobic treatment and the charge repulsive force between charged jets,liquid jets emanated from Cu grid kept their own tracks without aggregation.The initial diameter of liquid jet was greatly decreased by the Cu grid after hydrophobic treatment,and the smaller jet led to finer uniform nanofiber.Polyethylene oxide(PEO) and polyvinyl alcohol(PVA) ultrafine nanofiber with the diameter of 20—80 nm were fabricated by this novel spinneret,and the diameter of ultrafine nanofiber increases with the increase of polymer solution concentration.国家自然科学基金重点资助项目(51035002);国家自然科学基金资助项目(50875222

我国内蒙古大兴安岭北麓泡状肝包虫种类的研究I.多房棘球绦虫

人兽共患泡状(多房)棘球蚴病的病原种类,一向人们都认为只有多房棘球绦虫一种,其中包含分布在不同地区的2-3个地理株(或亚种)(Vogel,1957;KumaratilakeandThompson,1982;Miyazaki,1991;等)。但在我国内蒙古东北部大兴安岭北麓呼伦贝尔草原,却存在着多房棘球绦虫(EchinococcusmultilocularisLeuckart,1863)的所谓三个“地理株(或亚种)”:欧洲的多房棘球绦虫、冻土地带的西伯利亚棘球绦虫和苏俄的多房棘球绦虫。它们在内蒙古不仅存在于同一流行区,而且成虫常混合感染于同一终宿主沙狐的体内。2001年,作者再次检查呼伦贝尔的沙狐151只,从中检出19只感染有泡状棘球绦虫成虫,其中有9只混合感染上述的不同形态的三虫种。观察了它们成虫的埋蜡连续切片的制片,说明它们的子宫是各有稳定不变的结构。这三虫种经人工感染数百只实验鼠,比较观察了各虫体幼虫期大量的埋蜡连续切片,再次证实此三虫种在中间宿主鼠类体内发育的幼虫期,是各有一定的结构和发育格式。本文着重介绍欧洲型多房棘球绦虫的成虫子宫结构和它幼虫期发育

Effect of quasicrystalline phase on the deformation behavior of Zr62Al9.5Ni9.5Cu14Nb5 bulk metallic glass

Quasicrystalline phase with different volume fraction were formed by isothermally annealing the as-castZr(62)Al(9.5)Ni(9.5)Cu(14)Nb(5) bulk metallic glass at 723 K for different times. The effects of quasicrystals on the deformation behavior of the materials were studied by nanoindentation and compression test. It revealed that the alloys with homogeneous amorphous structure exhibit pronounced flow serrations during the nanoindentation loading, while no obvious flow serration is observed for the sample with quasicrystals more than 10 vol.%. However, further compression tests confirm that the no-serrated flows are formed due to different reasons. For annealed samples containing quasicrystals less than 35 vol.%, continuous plastic deformation occurs due to propagation of multiple shear bands. While the disappearance of serrated flow cannot be explained by the generation of multiple shear bands for samples containing quasicrystals more than 35 vol.%, which will fracture with a totally different fracture mode, namely, dimple fracture mode under loading instead of shear fracture mode. (c) 2005 Published by Elsevier B.V

我国内蒙古大兴安岭北麓泡状肝包虫种类的研究 III.苏俄棘球绦虫(Echinococcus russicensis sp.nov.)

在我国内蒙古大兴安岭北麓草场流行有与在前苏联广泛分布的泡状棘球蚴其成虫具球形子宫相似的病原,过去称之为苏俄的多房棘球绦虫。我们在比较研究了它与欧洲的多房棘球绦虫的全程生活史的发育规律,及成虫和幼虫期的结构之后,发现两者之间有极大差异,认为不能再用多房棘球绦虫称呼之。最近,我们用内蒙古的苏俄型泡状棘球蚴人工感染4只羊羔,全部阴性不能发育,说明内蒙古的本虫种不同于所谓幼虫期可以在牛羊发育的"哈萨克亚种"。为了纪念前苏联学者最早发现和叙述本种成虫,兹暂定名为苏俄棘球绦虫新种(Echinococcus russicensis sp.nov.)

我国内蒙古大兴安岭北麓泡状肝包虫种类的研究 II.西伯利亚棘球绦虫(Echinococcus sibiricensis Rauschet Schiller,1954)

目的Rausch and Schiller(1954)在美国阿拉斯加白令海峡St.Lawrence岛从北极狐(Alopex lagopus)和雪撬狗(sledge dogs)发现西伯利亚棘球绦虫新种(Echinococcus sibiricensis sp.nov.),但诸多学者认为E.sibiricensis Rausch et Schiller,1954是欧洲的多房棘球绦虫(Echinococcus multilocularis Leuckart,1863)的地理株(或亚种)(Vogel,1957;Skrjabin and Abuladze,1964;Kumaratilake and Thompson,1982;Meyajaki,1991等)。于1985-2002年间,作者在我国内蒙古大兴安岭北麓草场多年进行泡状棘球蚴(alveolar Echinococcus)病原的野外调查和实验室鼠类的人工感染试验,结果发现该地区同地点存在有北美西伯利亚棘球绦虫、欧洲多房棘球绦虫及苏俄多房棘球绦虫三种不同"地理株",而且三种成虫常混合感染于同一终宿主沙狐(Vulpes corsac)的体内,它们的幼虫期也存在于当地的布氏田鼠(Microtus brandti)。2001年,作者再次检查当地沙狐151只,从中检出19只感染有泡状棘球绦虫成虫。含成熟虫体的13只阳性沙狐中,感染西伯利亚棘球绦虫有11只,其中2只单独感染此虫种,另9只还混合感染了欧洲多房棘球绦虫及苏俄多房棘球绦虫三虫种。在呼伦贝尔草原的泡状棘球蚴病原中,西伯利亚棘球绦虫是优势种,它在终宿主沙狐和中间宿主的感染率都高过其他虫种。本文着重介绍西伯利亚棘球绦虫的成虫子宫结构和它幼虫期在野外布氏田鼠及人工感染实验鼠体内的发育情况